Repository: harvardinformatics/bioinformatics-coffee-hour Branch: master Commit: 17531934f410 Files: 107 Total size: 3.8 MB Directory structure: gitextract_0onxtofe/ ├── LICENSE ├── README.md ├── bedtools/ │ ├── binder/ │ │ └── environment.yml │ ├── data/ │ │ ├── H1-hESC-H3K27Ac_chr22.bed │ │ ├── hg38.genome │ │ ├── human_enhancers_chr22.bed │ │ └── ucscGenes_chr22.bed │ └── index.ipynb ├── differential-expression-analysis/ │ ├── binder/ │ │ └── Dockerfile │ ├── data/ │ │ ├── dme_elev_samples.tab │ │ └── dme_elevgrad_rsem_bt2_gene_counts.matrix.bz2 │ └── index.Rmd ├── enrichment-analysis/ │ ├── binder/ │ │ └── Dockerfile │ ├── data/ │ │ ├── dme_mapk_ensembl_geneids.txt │ │ └── heatshockprotein_ensembl_gene_ids.txt │ └── index.Rmd ├── ggplot-basics/ │ ├── binder/ │ │ └── Dockerfile │ └── index.Rmd ├── intro-to-awk/ │ ├── binder/ │ │ └── environment.yml │ ├── data/ │ │ ├── Homo_sapiens.GRCh38.subset.gff3 │ │ ├── Homo_sapiens_ucscGenes.subset.bed │ │ ├── enhancers.csv │ │ ├── example.fastq │ │ └── hg38.genome │ └── index.ipynb ├── intro-to-r/ │ ├── binder/ │ │ └── Dockerfile │ ├── data/ │ │ └── mbta_bus.tsv │ ├── index.Rmd │ └── instructor_copy.Rmd ├── intro_awk_spring2021/ │ ├── .ipynb_checkpoints/ │ │ └── index-checkpoint.ipynb │ ├── binder/ │ │ ├── .ipynb_checkpoints/ │ │ │ └── environment-checkpoint.yml │ │ └── environment.yml │ ├── data/ │ │ ├── .ipynb_checkpoints/ │ │ │ ├── Homo_sapiens.GRCh38.subset-checkpoint.gff3 │ │ │ ├── Homo_sapiens_ucscGenes.subset-checkpoint.bed │ │ │ ├── example-checkpoint.fastq │ │ │ └── hg38-checkpoint.genome │ │ ├── Homo_sapiens.GRCh38.subset.gff3 │ │ ├── Homo_sapiens_ucscGenes.subset.bed │ │ ├── dmel-subset-chromosomes.fasta │ │ ├── enhancers.csv │ │ ├── example.fastq │ │ ├── hg38.genome │ │ └── sample.txt │ └── index.ipynb ├── intro_data_science/ │ ├── part_1/ │ │ ├── binder/ │ │ │ └── requirements.txt │ │ ├── ds_part_1.ipynb │ │ └── ds_part_1_complete.ipynb │ ├── part_2/ │ │ ├── binder/ │ │ │ └── requirements.txt │ │ ├── ds_part_2.ipynb │ │ └── ds_part_2_complete.ipynb │ ├── part_3/ │ │ ├── binder/ │ │ │ └── requirements.txt │ │ ├── data/ │ │ │ ├── complete/ │ │ │ │ └── eur_data_final.csv │ │ │ ├── gdp_2016.csv │ │ │ ├── misc_data.xlsx │ │ │ ├── out/ │ │ │ │ ├── eur_data_sorted.csv │ │ │ │ ├── eur_data_sorted.html │ │ │ │ ├── eur_data_sorted.json │ │ │ │ └── eur_data_sorted.xlsx │ │ │ ├── unemployment_2016.csv │ │ │ └── ~$misc_data.xlsx │ │ ├── ds_part_3.ipynb │ │ └── ds_part_3_complete.ipynb │ └── part_4/ │ ├── binder/ │ │ └── requirements.txt │ ├── data/ │ │ └── complete/ │ │ ├── eur_data_final.csv │ │ └── un_world_data.csv │ ├── ds_part_4.ipynb │ └── ds_part_4_complete.ipynb ├── samtools/ │ ├── binder/ │ │ └── environment.yml │ ├── data/ │ │ ├── file.sorted.bam │ │ └── subset.bed │ ├── index.ipynb │ └── introduction.md ├── singularity/ │ ├── images/ │ │ ├── singularity_container.drawio │ │ ├── singularity_image.drawio │ │ └── trinity.drawio │ ├── part1.html │ └── part1.md ├── snakemake/ │ ├── LICENSE │ ├── README.md │ ├── Snakefile │ ├── binder/ │ │ ├── README.md │ │ └── environment.yml │ ├── data/ │ │ └── .gitignore │ └── index.ipynb ├── taste-of-conda/ │ ├── binder/ │ │ └── environment.yml │ └── index.ipynb ├── tidyverse/ │ ├── part1/ │ │ ├── binder/ │ │ │ └── Dockerfile │ │ └── index.Rmd │ ├── part1_winter2021/ │ │ ├── binder/ │ │ │ └── Dockerfile │ │ └── index.Rmd │ ├── part2/ │ │ ├── binder/ │ │ │ └── Dockerfile │ │ └── index.Rmd │ └── part2_winter2021/ │ ├── binder/ │ │ └── Dockerfile │ ├── co-est2019-annres.csv.xz │ ├── mask-use-by-county.csv │ ├── tidyverse_part2_winter2021.Rmd │ └── tidyverse_part2_winter2021.nb.html ├── tidyverse_covid_data/ │ ├── part1/ │ │ ├── binder/ │ │ │ └── Dockerfile │ │ ├── tidyverse_1.R │ │ └── tidyverse_1.Rmd │ └── part2/ │ ├── binder/ │ │ └── Dockerfile │ ├── co-est2019-annres.csv.xz │ ├── mask-use-by-county.csv │ ├── tidyverse_2.R │ └── tidyverse_2.Rmd └── unix-pipes/ ├── binder/ │ └── environment.yml └── index.ipynb ================================================ FILE CONTENTS ================================================ ================================================ FILE: LICENSE ================================================ MIT License Copyright (c) 2020 President and Fellows of Harvard College Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. ================================================ FILE: README.md ================================================ # Bioinformatics Coffee Hour | Date | Topic | Launch | | --- | --- | --- | | Tues, April 14, 2020 | [Intro to AWK](/intro-to-awk/index.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-04-14_intro-to-awk?urlpath=lab/tree/intro-to-awk/index.ipynb) | | Tues, April 21, 2020 | [Differential Expression Analysis with limma-voom](/differential-expression-analysis/index.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-04-21_differential-expression-analysis?urlpath=rstudio) | | Tues, April 28, 2020 | [A Taste of Conda](/taste-of-conda/index.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-04-28_taste-of-conda?urlpath=lab/tree/taste-of-conda/index.ipynb) | | Tues, May 5, 2020 | [Tidyverse (part 1)](/tidyverse/part1/index.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-05-05_tidyverse_part1?urlpath=rstudio) | | Tues, May 12, 2020 | [Tidyverse (part 2)](/tidyverse/part2/index.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-05-12_tidyverse_part2?urlpath=rstudio) | | Tues, May 19, 2020 | [Intro to bedtools](/bedtools/index.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-05-19_bedtools?urlpath=lab/tree/bedtools/index.ipynb) | | Tues, May 26, 2020 | [Snakemake](/snakemake/index.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-05-26_snakemake?urlpath=lab/tree/snakemake/index.ipynb) | | Tues, June 16, 2020 | [Unix Pipes](/unix-pipes/index.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-06-16_unix-pipes?urlpath=lab/tree/unix-pipes/index.ipynb) | | Tues, June 23, 2020 | [Samtools](/samtools/index.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-06-23_samtools?urlpath=lab/tree/samtools/index.ipynb) | | Tues, June 30, 2020 | [Enrichment Analysis for Bulk RNA-Seq with Camera and ROAST](/enrichment-analysis/index.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-06-30_enrichment-analysis?urlpath=rstudio) | | Tues, Aug 4, 2020 | [Intro to R](/intro-to-r/index.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-08-04_intro-to-r?urlpath=rstudio) | | Tues, Aug 11, 2020 | [Tidyverse with covid data 1](/tidyverse_covid_data/part1/tidyverse_1.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-08-11_tidyverse-with-covid-data-1?urlpath=rstudio) | | Tues, Aug 18, 2020 | [Tidyverse with covid data 2](/tidyverse_covid_data/part2/tidyverse_2.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-08-18_tidyverse-with-covid-data-2?urlpath=rstudio) | | Tues, Aug 25, 2020 | [ggplot basics](/ggplot-basics/index.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2020-08-25_ggplot-basics?urlpath=rstudio) | | Tues, January 19, 2021 | [Intro to Data Science - Part 1](/intro_data_science/part_1/ds_part_1.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2021-01-19_intro_data_science_1?urlpath=lab/tree/intro_data_science/part_1/ds_part_1.ipynb) | | Tues, January 26, 2021 | [Intro to Data Science - Part 2](/intro_data_science/part_2/ds_part_2.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2021-01-26_intro_data_science_2?urlpath=lab/tree/intro_data_science/part_2/ds_part_2.ipynb) | | Tues, February 2, 2021 | [Intro to Data Science - Part 3](/intro_data_science/part_3/ds_part_3.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2021-02-02_intro_data_science_3?urlpath=lab/tree/intro_data_science/part_3/ds_part_3.ipynb) | | Tues, February 9, 2021 | [Intro to Data Science - Part 4](/intro_data_science/part_4/ds_part_4.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2021-02-09_intro_data_science_4?urlpath=lab/tree/intro_data_science/part_4/ds_part_4.ipynb) | | Tues, March 2, 2021 | [Tidyverse (part 1) Winter 2021](/tidyverse/part1/index.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2021-03-02_tidyverse_part1?urlpath=rstudio) | | Tues, March 9, 2021 | [Tidyverse (part 2) Winter 2021](/tidyverse/part2_winter2021/tidyverse_part2_winter2021.Rmd) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2021-03-09_tidyverse_part2?urlpath=rstudio) | | Tues, April 20, 2021 | [Intro to AWK and Grep Spring 2021](/intro_awk_spring2021/index.ipynb) | [![Binder](https://mybinder.org/badge_logo.svg)](https://mybinder.org/v2/gh/harvardinformatics/bioinformatics-coffee-hour/2021-04-20_intro_awk?urlpath=lab/tree/intro_awk_spring2021/index.ipynb) | | Tues, April 27, 2021 | [Singularity (part 1): Finding and Running Containers](/singularity/part1.md) ================================================ FILE: bedtools/binder/environment.yml ================================================ channels: - conda-forge - bioconda - defaults dependencies: - bash_kernel - bedtools=2.29.2 ================================================ FILE: bedtools/data/H1-hESC-H3K27Ac_chr22.bed ================================================ chr22 16846128 16866063 . 295 chr22 16849975 16850195 . 703 chr22 16850703 16850930 . 1000 chr22 16861726 16861890 . 837 chr22 17047932 17048173 . 722 chr22 17306180 17306362 . 857 chr22 17535824 17546861 . 281 chr22 17573289 17612560 . 288 chr22 17617573 17634154 . 297 chr22 17639228 17640854 . 594 chr22 17651864 17653784 . 598 chr22 17667916 17686364 . 521 chr22 17674950 17675707 . 707 chr22 17678957 17680889 . 1000 chr22 17681072 17681213 . 1000 chr22 17695293 17695896 . 495 chr22 17698340 17699734 . 407 chr22 17708995 17726307 . 341 chr22 17736801 17743349 . 625 chr22 17743875 17751576 . 625 chr22 17753338 17754400 . 766 chr22 17766210 17774384 . 292 chr22 17778642 17802961 . 281 chr22 17788505 17788634 . 957 chr22 17790395 17790912 . 519 chr22 17790463 17790589 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ENST00000462238.5 0 + chr22 50783774 50788954 ENST00000463325.5 0 + chr22 50783796 50789353 ENST00000494075.5 0 + chr22 50783811 50789172 ENST00000423888.5 0 + chr22 50783849 50799441 ENST00000651346.1 0 + chr22 50783861 50801309 ENST00000480246.1 0 + chr22 50798654 50799123 ENST00000427528.1 0 + ================================================ FILE: bedtools/index.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "## Manipulating files with bedtoools\n", "### Bioinformatics Coffee Hour - May 18, 2020\n", "\n", "### Genomic Intervals\n", "Many types of bioinformatics data are stored as genomic intervals, that is ranges (potentially discontinuous) along a sequence. Of course this includes things like gene models, but also the output of peak callers, enhancer predictions, and many other things. This genomic interval information is typically stored in either GFF or bed files. Today we are going to focus on bed files. \n", "\n", "Bed files use a 0-based start (first base of a chromosome is 0), and a 1-based end. This means the interval from the first base to the hundredth base of a chromosome would be represented as start = 0, end = 100 in a bed file. Another way to think about this is that bed files are 0-based, half-open (the start is included in the interval but not the end). Note that GFF files use a different convention, just to make things more confusing. \n", "\n", "Bed files are just tab-delimited files with a fixed set of fields: \n", "\n", "|col|field|meaning|\n", "|:---|:---|:--|\n", "|1|seqname|chr or scaffold id interval is on|\n", "|2|start|start position (0-based) of interval|\n", "|3|end|end position (1-based) of interval|\n", "|4|name|name of interval|\n", "|5|score|score, e.g. for peak calls or other intervals with scores; can be .|\n", "|6|strand|strand interval is on for strand-specific intervals|\n", "|7|thickStart|graphical parameter for genome browser display|\n", "|8|thickEnd|graphical parameter for genome browser display|\n", "|9|itermRgb|graphical parameter for genome browser display|\n", "|10|blockCount|for multi-exon genes and similar|\n", "|11|blockSizes|for multi-exon genes and similar|\n", "|12|blockStarts| for multi-exon genes and similar|\n", "\n", "Only the first three fields are required, but you cannot skip fields (so if you want to include strand, you need to also include name and score in that order). This means that you can manipulate bed files with standard Unix tools (such as awk that we introduced a few weeks ago). However, many operations on intervals can get quite complicated, and so there are specialized tools for dealing with this specific kind of data. One of the most popular and useful is bedtools, which will be the focus of our demo today.\n", "\n", "First, we'll just look at an example bed file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/human_enhancers_chr22.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Finding the closest gene to something\n", "\n", "One very common task in interval manipulation is trying to find the closest interval from one file to intervals in another file, e.g. you have a list of putative ehnancers (as a bed file) and you want to know the closest gene to each one. In bedtools, this can be done very simply with the `closest` option:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools closest -a data/human_enhancers_chr22.bed -b data/ucscGenes_chr22.bed | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note we pipe the output to head with the `|` operator so that we only see the first few lines of the output.\n", "\n", "Many bedtools commands for comparing things use `-a` and `-b` to indicate the two files to compare. Although the exact meaning varies, usually this can be read as \"for each element in a, compare to all elements in b.\" So in this case, we get the closest gene to each enhancer. We could also do this in reverse, to get the closest enhancer for each gene." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools closest -a data/ucscGenes_chr22.bed -b data/human_enhancers_chr22.bed | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Notice that each element in the `-a` file only appears once, but elements in `-b` can appear many times. \n", "\n", "We might want to list not just the closest element, but how far away it is. We can do this using the `-d` option to bedtools closest, like so:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools closest -a data/human_enhancers_chr22.bed -b data/ucscGenes_chr22.bed -d | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now, the last column is the distance between the element in the A file and the element in the B file. Notice that some of these distances are 0, which means that the closest element in B overlaps the element in A. We might want to find the closest non-overlapping element, instead of the closest element. We can do this with the `-io` option:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools closest -a data/human_enhancers_chr22.bed -b data/ucscGenes_chr22.bed -d -io | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Like most bedtools tools, `closest` has a number of additional options to control filtering, reporting, and what is considered closest (e.g., requiring the same or different strand, or looking only at upstream or downstream features). You can see all the options with `-h` to display the help:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools closest -h" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "As an exercise, look through the help display and think about how you would find the closest **upstream** enhancer to each gene." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Finding overlaps\n", "\n", "Another common task is finding overlaps between two sets of intervals. For example, we might want to know which of our enhancers identified in neural crest cells are shared with enhancers identified in some other cell type. In your directory you should see another bed file, H1-hESC-H3K27Ac_chr22.bed, which has H3K27Ac peaks from human embryonic stem cells. We'll use bedtools to get the overlaps between this file and our human enhancers file.\n", "\n", "Intersect can be a little tricky to sort out the options, so we'll use this figure as reference:\n", "\n", "
", "\n", "\n", "Like `closest`, `bedtools intersect` uses a `-a` and a `-b` file. Each feature in A is compared to all features in B to identify overlaps. The simplest option is:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools intersect -a data/human_enhancers_chr22.bed -b data/H1-hESC-H3K27Ac_chr22.bed | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note that this only lists the features in `A` that have an overlap in `B`, and it doesn't tell us what they overlap. If we wanted to list all features in `A` and indicate what they overlap, we could use the `-wao` option:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools intersect -a data/human_enhancers_chr22.bed -b data/H1-hESC-H3K27Ac_chr22.bed -wao | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This output is a little complicated, so let's walk through it. The first 5 columns are the elements in A, and would look the same as `head data/human_enhancers_chr22.bed`. Next we have the element in B that overlaps, or `.` if no elements overlap. This is also a five-column bed file, ending with a score that is between 0 and 1000. Finally, we have an additional column that is the number of base pairs that overlap. Note that in this case, most of the elements in B are much bigger than the elements in A, so the overlap is typically the length of the element in A." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Let's say now we want to split our human_enhancers file into two separate files, one for those that overlap a H1-hESC-H3K27Ac and one for those that don't. We could do this using `awk` or `grep` on the output we just produced, but we can also do this with bedtools itself. There is an option, `-wa` that writes the original entry in A if it overlaps any entry in B, and other option `-v` that only reports elements in A with no overlap in B:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools intersect -a data/human_enhancers_chr22.bed -b data/H1-hESC-H3K27Ac_chr22.bed -wa -v | head" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools intersect -a data/human_enhancers_chr22.bed -b data/H1-hESC-H3K27Ac_chr22.bed -wa | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Bedtools intersect has **lots** of options, and usually there is a way to do anything that involves comparing to sets of intervals and finding overlaps with it." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Computing promoter intervals \n", "\n", "So far we've looked at examples of comparing two different files, but some bedtools options work with a single bed file. An example is the `flank` command, which creates a new interval adjacent to existing intervals. We can use this to, for example, compute promoter intervals for each gene, where we define promoters as the 2kb upstream of the start site:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools flank -i data/ucscGenes_chr22.bed -g data/hg38.genome -l 2000 -r 0 -s | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "One of the powerful features of bedtools is that you can combine different tools with the Unix `|`, to allow the output of one tool to be the input to another. We can use this to identify all the enhancers that fall within promoter regions of genes:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools flank -i data/ucscGenes_chr22.bed -g data/hg38.genome -l 2000 -r 0 -s | bedtools intersect -a data/human_enhancers_chr22.bed -b - -u | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Quick hits\n", "\n", "There are many other possibilities for what bedtools can do. We'll end with just a couple of more examples of how to solve common problems with bedtools." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "#### Make a bed file of intergenic regions from a bed file of genes" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools complement -i data/ucscGenes_chr22.bed -g data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "#### Randomly shuffle the location of enhancers, avoiding placing them in genes" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools shuffle -i data/human_enhancers_chr22.bed -g data/hg38.genome -excl data/ucscGenes_chr22.bed | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "#### Compute a single metric to measure similarity between two sets of intervals" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools flank -i data/ucscGenes_chr22.bed -g data/hg38.genome -l 2000 -r 0 -s | bedtools sort -i - | bedtools jaccard -a data/H1-hESC-H3K27Ac_chr22.bed -b -" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bedtools random -n 743 -l 2000 -g data/hg38.genome | bedtools sort -i - | bedtools jaccard -a data/H1-hESC-H3K27Ac_chr22.bed -b -" ] } ], "metadata": { "kernelspec": { "display_name": "Bash", "language": "bash", "name": "bash" }, "language_info": { "codemirror_mode": "shell", "file_extension": ".sh", "mimetype": "text/x-sh", "name": "bash" } }, "nbformat": 4, "nbformat_minor": 4 } ================================================ FILE: differential-expression-analysis/binder/Dockerfile ================================================ FROM rocker/binder:3.6.2 ARG NB_USER ARG NB_UID USER root # install dependencies RUN install2.r statmod \ && Rscript -e 'BiocManager::install("edgeR", update=FALSE)' \ && rm -rf /tmp/downloaded_files # copy files COPY ./differential-expression-analysis/ ${HOME} RUN chown -R ${NB_USER} ${HOME} USER ${NB_USER} ================================================ FILE: differential-expression-analysis/data/dme_elev_samples.tab ================================================ sample population temp SRR1576457 maine low SRR1576458 maine low SRR1576459 maine low SRR1576460 maine high SRR1576461 maine high SRR1576462 maine high SRR1576463 panama low SRR1576464 panama low SRR1576465 panama low SRR1576514 panama high SRR1576515 panama high SRR1576516 panama high ================================================ FILE: differential-expression-analysis/index.Rmd ================================================ --- title: 'Differential Expression Analysis with limma-voom' output: html_document: default --- # I. Preliminaries This tutorial consists of a workflow demostration for differential expression analyses using RNA-seq data using limma voom. While there are now many published methods for tackling specific steps, as well as full-blown pipelines, limma voom has been show to be one of the top performers with respect to controlling the false discovery rate. In our daylong differential expression workshop, we also cover DE testing with sleuth, another top performer with respect to FDR control, and which is designed explicitly to handle psueduo-alignment based estimates of abundance derived from kallisto. Our limma voom workflow will use a gene-level count matrix derived from estimates of RNA abundance in each samples. These estimates have been generated with RSEM, using bowtie2 read alignments. Our limma voom workflow will use a gene-level count matrix derived from estimates of RNA abundance in each samples. These estimates have been generated with RSEM, using bowtie2 read alignments. These alignments were to transcripts derived from an annotated reference genome. Specific topics covered today include: * Importing and pre-processing a count matrix * Using a sample table to define factors used in DE analysis * Analysis of single-factor designs * Analysis of 2-factor designs ## Sample data Our sample data comprises 12 paired-end RNA-seq libraries for whole body samples of *Drosophila melanogaster* from two geographic regions (Panama and Maine), with two temperature treatments ("low" ane "high") for each region, featuring three biological replicates for each region x treatment combination. Previously, these data were used to look for parallel gene expression patterns between high and low latitude populations (Zhao et al, 2015, *PLoS Genetics*) ## Loading required R libraries First, load all the R libraries that will be used for today's analyses: ```{r, echo=TRUE} library(edgeR) library(limma) ``` ## Data management 1. Load and view the table that associates sample IDs and treatments (dme_elev_samples.tab): ```{r, echo=TRUE} s2c<-read.table("data/dme_elev_samples.tab",header = TRUE, stringsAsFactors=FALSE) s2c ``` 2. Open RSEM matrix ```{r,echo=TRUE} rsem_gene_data<-read.table("data/dme_elevgrad_rsem_bt2_gene_counts.matrix.bz2",header=TRUE,row.names=1) ``` ## Pre-processing and filtering ### Handling non-integer RSEM estimates 3. Round the expression matrix ```{r,echo=TRUE} rnaseqMatrix=round(rsem_gene_data) ``` ### Filtering out lowly expressed genes 4. Create a boolean variable that classifies samples according to whether CPM>=1: ```{r,echo=TRUE} filter=rowSums(cpm(rnaseqMatrix)>=1)>=6 ``` 5. Apply the filter to the expression matrix: ```{r,echo=TRUE} cpm_filtered_matrix=rnaseqMatrix[filter,] ``` This operation filters out ~5000 genes, which reduces our multiple testing burden as well (although limma might not atually try and conduct tests on a subset of these). ## Creating a Digital Gene Expression list object To run limma, we need to transform the expression matrix into a DGElist ("digital gene expression list") which is an object class that comes from edgeR 6. Create the DGE object and normalized expression matrix: ```{r,echo=TRUE} DGE<-DGEList(cpm_filtered_matrix) ``` ## Normalization using TMM method 7. Calculate normalization factors and do MDS plot: ```{r,echo=TRUE} DGE<-calcNormFactors(DGE,method =c("TMM")) ``` ## Quick and dirty outlier check 8. Generate an MDS plot ```{r,echo=TRUE} tempvals<-s2c$temp plotMDS(DGE,top=500,col=ifelse(tempvals=="low","blue","red"),gene.selection="common") ``` ## The vanilla one factor, two treatment DE analysis A fundamental step of DE analysis is to construct a design matrix that will be used to fit the linear model. In our simple vanilla 2-condition, example we can simply use the temp variable in the s2c table to create a matrix. which represents binary 0/1 encodings of the temp conditions. 9. Create design matrix: ```{r,echo=TRUE} design_temp=model.matrix(~temp, data=s2c) design_temp ``` After creating the design matrix object, the standard approach is to next run limma voom on the DGE object, e.g.: ```{r,echo=TRUE,eval=FALSE} v <- voom(DGE, design=design_temp, plot=TRUE) ``` **However** ... while this works fine under an ideal scenario, it becomes a problem if there is variation in sample quality, or more generally, there is some indication that a subset of samples appear as outliers via MDS, PCA, etc. Particularly for RNA-seq experiments where researchers may only have a few repicates per sample, discarding outlier samples is not feasible because it may lead to few if any biological replciates for some subset of treatments. A better solution to this problem is to apply weights to samples such that outlier samples are downweighted during differential expression calcuations. Limma voom does this by calculating "empirical quality weights" for each sample. 10. Run limma voom with sample quality weights: ```{r,echo=TRUE} vwts <- voomWithQualityWeights(DGE, design=design_temp,normalize.method="none", plot=TRUE) ``` **Note:** we have already applied TMM normalization, thus can set the normalization argument to none. This above command will also generate a plot with two panels showing the mean-variance relationship fit on the left, and a barplot of weights assigned to individual samples. 11. Then, run the linear model fitting procedure 1st step: ```{r,echo=TRUE} fit=lmFit(vwts,design_temp) ``` 12. Then apply the empirical bayes procedure: ```{r,echo=TRUE} fit=eBayes(fit,robust=TRUE) ``` We use the robust=TRUE setting to leverage the quality weights such that the analysis is robust to outliers. One can then get a quick and dirty summary of how many genes are differentially expressed, setting the FDR threshold,where the "fdr" and "BH" methods are synonymous for Benjamini Hochberg adjusted p-values. 13. Get summary table: ```{r,echo=TRUE} summary(decideTests(fit,adjust.method="fdr",p.value = 0.05)) ``` One piece of important info is the factor relative to which logfold change is being calculated, i.e. low will be the numerator for logfold change calculations. 14. Explore the data by extracting the top 10 DE genes (sorted by p-value): ```{r,echo=TRUE} topTable(fit, adjust="BH",resort.by="P") ``` The full table will be useful for many purposes, such as creating custom MA or volcano plots with color-coding and symbols to meet your needs. 15. Create a table of all genes (significant and not) ```{r,echo=TRUE} all_genes<-topTable(fit, adjust="BH",coef="templow", p.value=1, number=Inf ,resort.by="P") ``` coeff = the coefficient or contrast you want to extract number = the max number of genes to list adjust = the P value adjustment method resort.by determines what criteria with which to sort the table ## Analysis of a 2-factor design Extending limma to analyze more complex designs is relatively straightforward. A key part is to specify the design matrix properly. For the 2-factor design, one would do this as follows: 16. Construct the design matrix to incorporate temperate and population effects ```{r,echo=TRUE} population <- factor(s2c$population) temperature <- factor(s2c$temp, levels=c("low","high")) design_2factor<- model.matrix(~population+temperature) design_2factor ``` Then, you would proceed with DE analysis in a similar fashion as with the single factor experiment described above. ================================================ FILE: enrichment-analysis/binder/Dockerfile ================================================ FROM rocker/binder:3.6.3 USER root # install dependencies RUN install2.r statmod \ && Rscript -e 'BiocManager::install("edgeR", update=FALSE)' \ && rm -rf /tmp/downloaded_files # copy files COPY --chown=rstudio:rstudio ./differential-expression-analysis/data ${HOME}/data/ COPY --chown=rstudio:rstudio ./enrichment-analysis/data ${HOME}/data/ COPY --chown=rstudio:rstudio ./enrichment-analysis/index.Rmd ${HOME} USER rstudio ================================================ FILE: enrichment-analysis/data/dme_mapk_ensembl_geneids.txt ================================================ geneid FBgn0261434 FBgn0015402 FBgn0243512 FBgn0024326 FBgn0014380 FBgn0003205 FBgn0004595 FBgn0011217 FBgn0024329 FBgn0014006 FBgn0262582 FBgn0046689 FBgn0003867 FBgn0039015 FBgn0003118 FBgn0267339 FBgn0015765 FBgn0000206 FBgn0001139 FBgn0039421 FBgn0039532 FBgn0086361 FBgn0001297 FBgn0003720 FBgn0003870 FBgn0010333 FBgn0010909 FBgn0016794 FBgn0014388 FBgn0262733 FBgn0003984 FBgn0014011 FBgn0026252 FBgn0015296 FBgn0004390 FBgn0004569 FBgn0003410 FBgn0259794 FBgn0052179 FBgn0003997 FBgn0003256 FBgn0264959 FBgn0003733 FBgn0011656 FBgn0033483 FBgn0001291 FBgn0004907 FBgn0004638 FBgn0013725 FBgn0015295 FBgn0010213 FBgn0021818 FBgn0023214 FBgn0086358 FBgn0003731 FBgn0016641 FBgn0265193 FBgn0004650 FBgn0000382 FBgn0003079 FBgn0000179 FBgn0003053 FBgn0005390 FBgn0029944 FBgn0265464 FBgn0030018 FBgn0010269 FBgn0002576 FBgn0003366 FBgn0010303 FBgn0261524 FBgn0003969 FBgn0030941 FBgn0026323 FBgn0263933 FBgn0000097 FBgn0000490 FBgn0283531 FBgn0003716 FBgn0000308 FBgn0004177 FBgn0044323 FBgn0003502 FBgn0020497 FBgn0001137 FBgn0003209 FBgn0003751 FBgn0032210 FBgn0015399 FBgn0024846 FBgn0001965 FBgn0000153 FBgn0005672 ================================================ FILE: enrichment-analysis/data/heatshockprotein_ensembl_gene_ids.txt ================================================ geneid FBgn0010247 FBgn0010173 FBgn0024330 FBgn0037878 FBgn0013275 FBgn0013276 FBgn0013277 FBgn0051354 FBgn0013278 FBgn0013279 FBgn0026207 FBgn0001217 FBgn0038145 FBgn0004237 FBgn0038274 FBgn0266599 FBgn0038578 FBgn0020238 FBgn0263738 FBgn0263757 FBgn0038609 FBgn0038722 FBgn0028396 FBgn0044812 FBgn0038838 FBgn0044809 FBgn0044810 FBgn0039004 FBgn0024509 FBgn0039120 FBgn0039125 FBgn0001230 FBgn0039301 FBgn0039562 FBgn0035110 FBgn0035243 FBgn0011573 FBgn0001233 FBgn0263106 FBgn0035852 FBgn0001229 FBgn0001223 FBgn0001225 FBgn0001227 FBgn0001224 FBgn0001226 FBgn0036263 FBgn0086708 FBgn0001216 FBgn0026418 FBgn0263606 FBgn0004401 FBgn0266580 FBgn0033247 FBgn0033264 FBgn0013756 FBgn0033737 FBgn0028683 FBgn0001220 FBgn0034091 FBgn0034118 FBgn0001222 FBgn0034310 FBgn0040273 FBgn0034646 FBgn0034707 FBgn0034722 FBgn0034795 FBgn0010660 FBgn0011296 FBgn0013548 FBgn0002174 FBgn0034939 FBgn0023529 FBgn0003371 FBgn0029676 FBgn0260484 FBgn0013987 FBgn0028982 FBgn0011661 FBgn0266421 FBgn0001218 FBgn0061200 FBgn0010602 FBgn0011244 FBgn0031701 FBgn0031728 FBgn0027868 FBgn0021761 FBgn0262647 FBgn0032474 FBgn0032525 FBgn0086691 FBgn0032586 FBgn0044811 FBgn0053117 FBgn0032906 ================================================ FILE: enrichment-analysis/index.Rmd ================================================ --- title: 'Enrichment analysis for bulk RNA-seq with Camera and Roast' output: html_document: default --- # I. Preliminaries This tutorial builds on a workflow presented in our last office hours tutorial on conducting bulk RNA-seq analyses with limma voom. In that tutorial, we analyzed gene-level expression estimates for an experiment looking at parallel climate adaptation in *Drosophila melanogaster*. Today, we introduce methods available in the limma voom R package for conducting enrichment tests, specifically evaluating the extent of DE in a set of features (isoforms or genes), that typically correspond to a pathway of interest. These methods have been developed specifically for expression analyses, including RNA-seq, in order to explicitly account for the correlation among expression levels across assayed features, i.e. isoforms or genes. CAMERA performs competitive tests, that compare the extent of DE in a feature set compared to a background set. ROAST performs focused tests on feature sets, to test the hypothesis that there is a significant level of DE observed in features within the set. We will specifically use mROAST that performs multiple single-set tests while adjusting for multiple comparisons. These methods are more appropriate for RNA-seq experiments than GSEA, because GSEA calculates P-values by permuting sample ids. When there are few samples, as is the case in most bulk RNA-seq experiments, sample permutations lead to elevated false discovery rates. Specific topics covered today include: * Quick review of steps for obtaining DE results with limma voom * Running CAMERA on limma voom results for MAPK pathway genes involved in stress response and heat shock proteins * Running mROAST on limma voom DE results on two gene sets: MAPK pathway genes and heat shock proteins ## Sample data Our sample data comprises 12 paired-end RNA-seq libraries for whole body samples of *Drosophila melanogaster* from two geographic regions (Panama and Maine), with two temperature treatments ("low" and "high") for each region, featuring three biological replicates for each region x treatment combination. Previously, these data were used to look for parallel gene expression patterns between high and low latitude populations (Zhao et al, 2015, *PLoS Genetics*) ## Loading required R libraries First, load all the R libraries that will be used for today's analyses: ```{r, echo=TRUE} library(edgeR) library(limma) library(statmod) ``` ## Data management 1. Load and view the table that associates sample IDs and treatments (dme_elev_samples.tab): ```{r, echo=TRUE} s2c<-read.table("data/dme_elev_samples.tab",header = TRUE, stringsAsFactors=FALSE) s2c ``` 2. Open RSEM matrix ```{r,echo=TRUE} rsem_gene_data<-read.table("data/dme_elevgrad_rsem_bt2_gene_counts.matrix.bz2",header=TRUE,row.names=1) ``` ## Pre-processing and filtering ### Handling non-integer RSEM estimates 3. Round the expression matrix ```{r,echo=TRUE} rnaseqMatrix=round(rsem_gene_data) ``` ### Filtering out lowly expressed genes 4. Create a boolean variable that classifies samples according to whether CPM>=1: ```{r,echo=TRUE} filter=rowSums(cpm(rnaseqMatrix)>=1)>=6 ``` 5. Apply the filter to the expression matrix: ```{r,echo=TRUE} cpm_filtered_matrix=rnaseqMatrix[filter,] ``` ## Creating a Digital Gene Expression list object To run limma, we need to transform the expression matrix into a DGElist ("digital gene expression list") which is an object class that comes from edgeR 6. Create the DGE object and normalized expression matrix: ```{r,echo=TRUE} DGE<-DGEList(cpm_filtered_matrix) ``` ## Normalization using TMM method 7. Calculate normalization factors and do MDS plot: ```{r,echo=TRUE} DGE<-calcNormFactors(DGE,method =c("TMM")) ``` ## Analysis of a 2-factor design Extending limma to analyze more complex designs is relatively straightforward. A key part is to specify the design matrix properly. For the 2-factor design, one would do this as follows: 8. Construct the design matrix to incorporate temperate and population effects ```{r,echo=TRUE} population <- factor(s2c$population) temperature <- factor(s2c$temp, levels=c("low","high")) design_2factor<- model.matrix(~population+temperature) design_2factor ``` 9. Run limma voom with sample quality weights: ```{r,echo=TRUE} vwts <- voomWithQualityWeights(DGE, design=design_2factor,normalize.method="none", plot=TRUE) ``` 10. Then, run the linear model fitting procedure 1st step: ```{r,echo=TRUE} fit=lmFit(vwts,design_2factor) ``` 11. Then apply the empirical bayes procedure: ```{r,echo=TRUE} fit=eBayes(fit,robust=TRUE) ``` 12. Get summary table of all tests, including NS results: ```{r,echo=TRUE} all_genes<-topTable(fit, adjust="BH",coef="temperaturehigh", p.value=1, number=Inf ,resort.by="P") ``` II. Running Camera 13. load table of KEGG-classfied MAPK pathway genes ```{r} mapk<-read.table("data/dme_mapk_ensembl_geneids.txt",header=TRUE) head(mapk) ``` 14. create vector of row (gene) indices for the heat shock protein genes: ```{r,echo=TRUE} mapk_indices<-ids2indices(mapk,row.names(cpm_filtered_matrix)) ``` CameraPR runs camera on precomputed test statistics such at the t-statistics in the limma fit object, so is directly applicable to our limma pipeline. 15. run Camera on the precomputed limma fit object It is important to use the correct column of the design matrix. ```{r,echo=TRUE} colnames(design_2factor) ``` As we can see, column 3 is for the temperature factor that we are interested in ```{r,echo=TRUE} temp_camera_pr<-cameraPR(fit$t[,3],mapk_indices) temp_camera_pr ``` **Note:** The current default for inter.gene.cor is 0.01, because in the words of the authors, with this value "camera will rank biologically interpretable sets more highly. This gives a useful compromise between strict error rate control and interpretable gene set rankings." This is particularly applicable to relative rankings when one supplies several sets for testing. As it turns out, one can't change this setting in cameraPR. If one wants the rigorous error rate control that results from estimating the inter-gene correlation, and if one isn't worried about relative rankings of different sets, one could call camera, and set inter.gene.cor to NULL, which makes it estimate that correlation. With camera, you need to be careful to specify the relevant expression matrix. The raw input is not necessarily appropriate in this case, as DE testing is done on TMM normalized, quality-weighted data, which we can access via the vmwts object: 16. Run Camera with inter-gene correlation estimation ```{r,echo=TRUE} temp_camera_wcorest<-camera(vwts$E,mapk_indices,design_2factor,contrast="temperaturehigh",inter.gene.cor=NA) temp_camera_wcorest ``` When camera is asked to do estimation, it returns the estimated correlation in the output. The estimated correlation coefficient is substantially larger than the default used with cameraPR, leading to a more conservative P-value, and a non-significant result. With either cameraPR, or camera, if you provide multiple gene sets, an FDR value will also be provided. 17. Load the heat shock proteins table ```{r,echo=TRUE} hsps<-read.table("data/heatshockprotein_ensembl_gene_ids.txt",header=TRUE) ``` 18. Create heat shock protein indices ```{r,ech=TRUE} hsps_indices<-ids2indices(hsps,row.names(cpm_filtered_matrix)) ``` 19. Run Camera with inter-gene correlation estimation for both gene sets ```{r,echo=TRUE} temp_camera_wcorest_2sets<-camera(vwts$E,index=list(mapk=mapk_indices$geneid,heatshock=hsps_indices$geneid),design_2factor,contrast="temperaturehigh",inter.gene.cor=NA) temp_camera_wcorest_2sets ``` Our results demonstrate a potential pitfall of using default behavior of an enrichment tool, by assuming a weak correlation among pathway genes! **NOTE:** it is entirely possible to run camera on expression data or differential expression test results derived from another pipeline. For example, one could supply an expression matrix derived from kallisto estimates, and a design matrix, and use camera. Perhaps more ideal, to take advantage of the advantages sleuth offers for kallisto-derived estimates, one could supply test statistics obtained from sleuth and run cameraPR. III. Running targeted enrichment analyses with mRoast 20. Run mRoast on heat shock protein and MAPK pathway gene sets ```{r,echo=TRUE} roast_results<-mroast(vwts$E,index=list(mapk = mapk_indices$geneid, heatshock = hsps_indices$geneid),design=design_2factor,contrast="temperaturehigh",adjust.method="BH",set.statistic="mean50") roast_results ``` Roast provides P-values and adjusted P-values (FDR) for the direction with the strongest signal, as well as the significance of the combination of both up and down regulation relative to the factor-level that the coefficient in the design describes, in our case "temperaturehigh". There are a number of arguments one can change, but perhaps the most important is "set.statistic" which defines the summary statistic on which significant testing is performed. The right option depends in part on prior knowledge of the extent of DE in the gene set. "mean.50" performs well under a variety of conditions, representing a balance between power and false discovery and will detect as few as 25% differentially expressed genes. When it is expected that only a small fraction of genes in the set will be differentially expressed, "msq" is thought to perform better. "Mean" should only be used when a majority of genes are expected to be differentially expressed.For out data set, choosing between these two statistics only has minimal effect on P-values, and resulting test-specific FDR estimates. In the big picture, Camera competitive tests show there is not significant enrichment of DE signals in the two pathways relative ot overall patterns of DE. However, there are so many differentially expressed genes in this experiment, that this may obscure interesting biology! In contrast, Roast detects DE in both pathways. Perhaps more informative would be an analysis of a larger set of pathway gene sets, and examining how the fractions of DE genes differ amongst pathways to highlight pathways with particularly strong signal. ================================================ FILE: ggplot-basics/binder/Dockerfile ================================================ FROM rocker/binder:3.6.3 ARG NB_USER USER root RUN install2.r dslabs \ && rm -rf /tmp/downloaded_packages USER ${NB_USER} COPY --chown=${NB_USER} ./ggplot-basics/ ${HOME} ================================================ FILE: ggplot-basics/index.Rmd ================================================ --- title: "ggplot basics" subtitle: "Bioinformatics Coffee Hour" date: "August 25, 2020" author: "Tim Sackton" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` The goal of this workshop is to teach the grammar of graphics in R, with a focus on **ggplot2**. The consistent grammar implemented in ggplot2 is advantageous both because it is easily extendible - that is you can both produce simple plots, but then develop them into complex publication-ready figures. In addition to the basic ggplot2 R package, many extensions for different types of data have been written using the same standardized grammar. ggplot2 is part of the tidyverse package, and to make it easier to load our dataset and manipulate it prior to plotting, we will load the entire tidyverse package. ```{r, echo=FALSE} library(tidyverse) library(dslabs) ``` # Basic grammar of ggplot Today we will be introducing the basics of ggplot, using a variety of datasets from the 'dslabs' package, which includes data that has already been cleaned and tidied, and is appropriate for various plotting tasks. There are three key components that make up every ggplot: 1. **data** 2. **aesthetic mappings** (which variables in your data map to which visual properties) 3. **geometric object (geom) function** (a layer describing how to render each observation) There are other optional components that control the visualization of the plots, but for now, we will focus on getting these three key elements down. The basic formula for these options is: `ggplot(data=, aes()) + ()` Let's make a basic plot using this grammar. You can see that this is really not much more complicated than the base R *plot* function. We'll use a dataset from the website [Spurious Correlations](https://www.tylervigen.com/spurious-correlations), which is amusing to browse and demonstrates the limitations of inference from correlations along. ```{r, echo=FALSE} data(divorce_margarine) ggplot(data=divorce_margarine, aes(x=divorce_rate_maine, y=margarine_consumption_per_capita)) + geom_point() ``` Let's break down what this is actually doing a little bit, to try to get an inuititive understanding for how the 'grammer of graphics' works. The initial ggplot plot command sets up a coordinate axis. We can just run this, without the geom_point() command, to see what happens: ```{r fig.show='asis'} ggplot(divorce_margarine, aes(x=divorce_rate_maine, y=margarine_consumption_per_capita)) ``` Note that this just sets up the coordinate system: no points are plotted. The plotting happens only when we *add* geom_point() to the plot. So each ggplot is set up as layers: first, a coordinate system, and then one or more overlays plotting data on that coordinate system. This has a few implications for how to think about plots. First, it means that you can layer multiple plots on single coordinate system -- we'll see how to do this in a minute. Second, it means that you can save a coordinate system as an object, and then add different plots to it. We'll do both these things now. ```{r echo=TRUE} spurCor <- ggplot(divorce_margarine, aes(x=divorce_rate_maine, y=margarine_consumption_per_capita)) spurCor + geom_point() spurCor + geom_point() + geom_smooth() ``` Note, this adds a y ~ x line to our plot, on top of the scatterplot. If we take our spurCor coordinate system, we can also just add a line, without the scatterplot, by adding geom_smooth(). We'll also add some options here, instead of using the defaults. In this case, we are using a linear model (method="lm"), with a simple y = x formula. ```{r echo=FALSE} spurCor + geom_smooth(method = "lm", formula = y ~ x) ``` Again, remember the basic framework is: 1. **data** 2. **aesthetic mappings** (which variables in your data map to which visual properties) 3. **geometric object (geom) function** (a layer describing how to render each observation) In this case, we've stored the data and aesthetic mappings/coordinate system in the spurCor object. As an aside: ggplot includes a huge number of options for controlling how plots look - colors, background, axis, labels, titles, legends, and more. In addition, these options can be wrapped in themes that apply a consistent look to all your graphs. While we will see a few of these in operation in the next few examples, in the interest of time we won't be able to cover all the possible options for controlling the look of graphics today. However, you can look at some [other](http://r-statistics.co/Complete-Ggplot2-Tutorial-Part2-Customizing-Theme-With-R-Code.html) [tutorials](http://zevross.com/blog/2014/08/04/beautiful-plotting-in-r-a-ggplot2-cheatsheet-3/). Next, let's look at a slightly more complicated dataset and see some basic customization options. We'll use another dataset available from the dslabs package, the gapminder package. ```{r} data(gapminder) ggplot(gapminder, aes(x=life_expectancy, y=gdp/population)) + geom_point() ``` A few things to note. First of all, we can define new variables in the aes statement, using syntax like the tidyverse mutate() command. This can be very useful. Second, though, this plot doesn't look so good. There are lots of points, they are overplotted on top of each other, just plain black is boring, and there is a lot more data in our dataset we might want to know about. Let's do this again, but introduce some customization options. ```{r} gapminder %>% filter(year == 2000, !is.na(gdp)) %>% # let's just look at one year mutate(gdpPerCap = gdp/population) %>% ggplot(aes(x = life_expectancy, y=gdpPerCap, color=continent)) + geom_point() ``` We've added a few things here. First, we did some filtering and rearranging with tidyverse before sending the data to ggplot. Second, we added a color = to the aes() code, which tells ggplot to color each point based on the value of that variable. Let's try these two changes. First, we'll keep the same plot as above, but make all the points red. Second, we'll look at Europe by year, and color the points by GDP ```{r} gapminder %>% filter(year==2000, !is.na(gdp)) %>% mutate(gdpPerCap = gdp/population) %>% ggplot(aes(x = life_expectancy, y=gdpPerCap)) + geom_point(color="red") gapminder %>% filter(continent == "Europe", !is.na(gdp)) %>% mutate(gdpPerCap = gdp/population) %>% ggplot(aes(y = life_expectancy, x=year, color=gdpPerCap)) + geom_point() ``` Note that we put the color option in geom_point() if we wanted everything to be the same color. This sets the color for that layer only. This means we can do things like this: ```{r} gapminder %>% filter(year==2000, !is.na(gdp)) %>% mutate(gdpPerCap = gdp/population) %>% ggplot(aes(x = life_expectancy, y=log10(gdpPerCap), color=continent)) + geom_point(color="black", alpha = 0.2) + geom_smooth(method="lm", formula = y ~ x, se = FALSE) ``` In this case, this may not be a particularly useful graph, but note two things: * The update to the aesthetics (changing color to "black") in a geom statement override the default aes we call in the ggplot command * This doesn't persist to the next layer, which goes back to using the default aesthetics This means we can change certain parts of the aesthetic on a layer by layer basis. Another way to change the aesthetic is by using themes, which replace a bunch of aesthetics. Here are few examples; there are tons more available in other packages, e.g. the ggthemes package, to help you customize your plots. You can also make your own, but that is beyond the scope of this tutorial. I will also add a command to scale the y axis to be log10 scale. ```{r} gdp_v_le <- gapminder %>% filter(year==2000, !is.na(gdp)) %>% mutate(gdpPerCap = gdp/population) %>% ggplot(aes(x = life_expectancy, y=gdpPerCap, color=continent)) + geom_point(color="black", alpha = 0.2) + geom_smooth(method="lm", formula = y ~ x, se = FALSE) + scale_y_log10() gdp_v_le + theme_dark() gdp_v_le + theme_classic() gdp_v_le + theme_minimal() ``` So far, we've only looked at plots involving two dimensional continous data, such as scatter and line plots. Let's now look at some additional types of plots. ## 1-d plots The simplest kind of one-dimensional plots are those that summarize the distribution of a single continuous variable, such as histograms and density plots. These are very useful, so let's look at how to make them with ggplot. ```{r} gapminder %>% ggplot(aes(life_expectancy)) + geom_histogram(binwidth=2) ``` ```{r} le_dist <- gapminder %>% mutate(decade = as.factor(floor(year/10))) %>% ggplot(aes(life_expectancy, color=decade)) le_dist + geom_histogram(binwidth = 3) le_dist + geom_freqpoly(binwidth = 3) le_dist + geom_density() ``` ## Categorical data Sometimes we want to plot categorical data, either bar plots with counts of each discrete value, or boxplots or similar that summarize the distribution of a continuous variable by category. For example, we might want to look at a summary of the plot we just made. ```{r} gapminder %>% mutate(decade = as.factor(floor(year/10))) %>% ggplot(aes(x=decade, y=life_expectancy)) + geom_boxplot() gapminder %>% mutate(decade = as.factor(floor(year/10))) %>% ggplot(aes(x=decade, y=life_expectancy, fill=continent)) + geom_boxplot() gapminder %>% mutate(decade = as.factor(floor(year/10))) %>% ggplot(aes(x=continent, y=life_expectancy)) + geom_boxplot(aes(color=decade)) ``` Notice that the 'color' argument here in aes() functions like a group_by argument, and groups points by default; fill does the same thing but colors the inside of the boxplot instead of the outside. Note also that we can put the aes() argument either in the ggplot call or the geom. For this plot, it doesn't make a difference, but if we wanted to include multiple geoms in one plot, it could. Finally, let's end with something a bit more complicated: ```{r} gapminder %>% mutate(decade = as.factor(floor(year/10)), log_gdpPerCap = log10(gdp/population)) %>% filter(!is.na(log_gdpPerCap)) %>% ggplot(aes(x=log_gdpPerCap, y=life_expectancy)) + geom_point(alpha=0.2, color="gray50") + geom_smooth(color="red", method="loess", formula = y~x) + facet_grid(cols=vars(decade), rows=vars(continent)) ``` ## Review We've covered a lot of material here, so let's just touch on the basics. A plot in ggplot is built up from a dataset, a set of aesthetics establishing a coordinate system and axis, and geoms mapping data to the aesthetics. For many simple plots, the default options for the geom call work fine, as we've seen. There are geoms for almost any way of plotting data you could imagine. Grouping is available as well, which lets you naturally summarize subsets of your data. While we didn't talk about it today, facet_grid and facet_wrap can help you create grids of plots each summarizing a different subset of the data. ================================================ FILE: intro-to-awk/binder/environment.yml ================================================ dependencies: - bash_kernel ================================================ FILE: intro-to-awk/data/Homo_sapiens.GRCh38.subset.gff3 ================================================ ##gff-version 3 ##sequence-region 1 1 248956422 1 Ensembl chromosome 1 248956422 . . . ID=chromosome:1 1 . biological_region 10469 11240 1.3e+03 . . external_name=oe %3D 0.79 1 . biological_region 10650 10657 0.999 + . logic_name=eponine 1 . biological_region 10655 10657 0.999 - . logic_name=eponine 1 . biological_region 10678 10687 0.999 + . logic_name=eponine 1 . biological_region 10681 10688 0.999 - . logic_name=eponine 1 . biological_region 10707 10716 0.999 + . logic_name=eponine 1 . biological_region 10708 10718 0.999 - . logic_name=eponine 1 . biological_region 10735 10747 0.999 - . logic_name=eponine 1 . biological_region 10737 10744 0.999 + . logic_name=eponine 1 . biological_region 10766 10773 0.999 + . logic_name=eponine 1 . biological_region 10770 10779 0.999 - . logic_name=eponine 1 . biological_region 10796 10801 0.999 + . logic_name=eponine 1 . biological_region 10810 10819 0.999 - . logic_name=eponine 1 . biological_region 10870 10872 0.999 + . logic_name=eponine 1 . biological_region 10889 10893 0.999 - . logic_name=eponine 1 havana pseudogene 11869 14409 . + . ID=gene:ENSG00000223972 1 havana lnc_RNA 11869 14409 . + . ID=transcript:ENST00000456328 1 havana exon 11869 12227 . + . Parent=transcript:ENST00000456328 1 havana exon 12613 12721 . + . Parent=transcript:ENST00000456328 1 havana exon 13221 14409 . + . Parent=transcript:ENST00000456328 1 havana pseudogenic_transcript 12010 13670 . + . ID=transcript:ENST00000450305 1 havana exon 12010 12057 . + . Parent=transcript:ENST00000450305 1 havana exon 12179 12227 . + . Parent=transcript:ENST00000450305 1 havana exon 12613 12697 . + . Parent=transcript:ENST00000450305 1 havana exon 12975 13052 . + . Parent=transcript:ENST00000450305 1 havana exon 13221 13374 . + . Parent=transcript:ENST00000450305 1 havana exon 13453 13670 . + . Parent=transcript:ENST00000450305 1 havana pseudogene 14404 29570 . - . ID=gene:ENSG00000227232 1 havana pseudogenic_transcript 14404 29570 . - . ID=transcript:ENST00000488147 1 havana exon 14404 14501 . - . Parent=transcript:ENST00000488147 1 havana exon 15005 15038 . - . Parent=transcript:ENST00000488147 1 havana exon 15796 15947 . - . Parent=transcript:ENST00000488147 1 havana exon 16607 16765 . - . Parent=transcript:ENST00000488147 1 havana exon 16858 17055 . - . Parent=transcript:ENST00000488147 1 havana exon 17233 17368 . - . Parent=transcript:ENST00000488147 1 havana exon 17606 17742 . - . Parent=transcript:ENST00000488147 1 havana exon 17915 18061 . - . Parent=transcript:ENST00000488147 1 havana exon 18268 18366 . - . Parent=transcript:ENST00000488147 1 havana exon 24738 24891 . - . Parent=transcript:ENST00000488147 1 havana exon 29534 29570 . - . Parent=transcript:ENST00000488147 1 . biological_region 15796 16060 0.999 - . external_name=rank %3D 1 1 mirbase ncRNA_gene 17369 17436 . - . ID=gene:ENSG00000278267 1 mirbase miRNA 17369 17436 . - . ID=transcript:ENST00000619216 1 mirbase exon 17369 17436 . - . Parent=transcript:ENST00000619216 1 . biological_region 28736 29810 1.01e+03 . . external_name=oe %3D 0.88 1 . biological_region 29116 29118 0.999 + . logic_name=eponine 1 . biological_region 29127 29206 1 + . external_name=rank %3D 1 1 . biological_region 29321 29395 1 - . external_name=rank %3D 1 1 . biological_region 29394 29396 0.999 - . logic_name=eponine 1 . biological_region 29448 29451 0.999 + . logic_name=eponine 1 havana ncRNA_gene 29554 31109 . + . ID=gene:ENSG00000243485 1 havana lnc_RNA 29554 31097 . + . ID=transcript:ENST00000473358 1 havana exon 29554 30039 . + . Parent=transcript:ENST00000473358 1 havana exon 30564 30667 . + . Parent=transcript:ENST00000473358 1 havana exon 30976 31097 . + . Parent=transcript:ENST00000473358 1 havana lnc_RNA 30267 31109 . + . ID=transcript:ENST00000469289 1 havana exon 30267 30667 . + . Parent=transcript:ENST00000469289 1 havana exon 30976 31109 . + . Parent=transcript:ENST00000469289 1 . biological_region 29583 29584 0.999 - . logic_name=eponine 1 mirbase ncRNA_gene 30366 30503 . + . ID=gene:ENSG00000284332 1 mirbase miRNA 30366 30503 . + . ID=transcript:ENST00000607096 1 mirbase exon 30366 30503 . + . Parent=transcript:ENST00000607096 1 havana ncRNA_gene 34554 36081 . - . ID=gene:ENSG00000237613 1 havana lnc_RNA 34554 36081 . - . ID=transcript:ENST00000417324 1 havana exon 34554 35174 . - . Parent=transcript:ENST00000417324 1 havana exon 35277 35481 . - . Parent=transcript:ENST00000417324 1 havana exon 35721 36081 . - . Parent=transcript:ENST00000417324 1 havana lnc_RNA 35245 36073 . - . ID=transcript:ENST00000461467 1 havana exon 35245 35481 . - . Parent=transcript:ENST00000461467 1 havana exon 35721 36073 . - . Parent=transcript:ENST00000461467 1 . biological_region 35904 36086 0.879 - . external_name=rank %3D 1 1 havana pseudogene 52473 53312 . + . ID=gene:ENSG00000268020 1 havana pseudogenic_transcript 52473 53312 . + . ID=transcript:ENST00000606857 1 havana exon 52473 53312 . + . Parent=transcript:ENST00000606857 1 havana pseudogene 57598 64116 . + . ID=gene:ENSG00000240361 1 havana lnc_RNA 57598 64116 . + . ID=transcript:ENST00000642116 1 havana exon 57598 57653 . + . Parent=transcript:ENST00000642116 1 havana exon 58700 58856 . + . Parent=transcript:ENST00000642116 1 havana exon 62916 64116 . + . Parent=transcript:ENST00000642116 1 havana pseudogenic_transcript 62949 63887 . + . ID=transcript:ENST00000492842 1 havana exon 62949 63887 . + . Parent=transcript:ENST00000492842 1 ensembl_havana gene 65419 71585 . + . ID=gene:ENSG00000186092 1 havana mRNA 65419 71585 . + . ID=transcript:ENST00000641515 1 havana exon 65419 65433 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 65419 65433 . + . Parent=transcript:ENST00000641515 1 havana exon 65520 65573 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 65520 65573 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 69037 69090 . + . Parent=transcript:ENST00000641515 1 havana exon 69037 71585 . + . Parent=transcript:ENST00000641515 1 havana CDS 69091 70008 . + 0 ID=CDS:ENSP00000493376 1 havana three_prime_UTR 70009 71585 . + . Parent=transcript:ENST00000641515 1 ensembl mRNA 69055 70108 . + . ID=transcript:ENST00000335137 1 ensembl five_prime_UTR 69055 69090 . + . Parent=transcript:ENST00000335137 1 ensembl exon 69055 70108 . + . Parent=transcript:ENST00000335137 1 ensembl CDS 69091 70008 . + 0 ID=CDS:ENSP00000334393 1 ensembl three_prime_UTR 70009 70108 . + . Parent=transcript:ENST00000335137 1 ensembl_havana ncRNA_gene 89295 133723 . - . ID=gene:ENSG00000238009 1 havana lnc_RNA 89295 120932 . - . ID=transcript:ENST00000466430 1 havana exon 89295 91629 . - . 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chr1 40713572 40717365 uc001cfe.2 chr1 40723721 40759856 uc001cfg.4 chr1 40766162 40782981 uc001cfh.1 chr1 40839377 40888998 uc001cfj.3 chr1 40916336 40929390 uc001cfn.2 chr1 40943301 40962015 uc001cfo.3 chr1 40974432 40982214 uc001cfp.3 chr1 40997232 41013841 uc001cft.2 chr1 41086351 41131324 uc001cfu.1 chr1 41154751 41157933 uc010ojl.1 chr1 41157241 41237275 uc009vwd.3 chr1 41220026 41220118 uc001cgf.2 chr1 41222955 41223044 uc001cgg.3 chr1 41249683 41306124 uc001cgh.2 chr1 41326727 41328018 uc001cgj.3 chr1 41347313 41347427 uc021omc.1 chr1 41445006 41478235 uc001cgk.4 chr1 41480261 41509562 uc021omd.1 chr1 41481268 41487427 uc001cgm.2 chr1 41492870 41707815 uc001cgs.3 chr1 41932607 41932699 uc021ome.1 chr1 41944445 41949874 uc009vwh.3 chr1 41944445 41950344 uc001cgx.3 chr1 41972035 42384496 uc001cha.4 chr1 42619091 42621495 uc001chc.1 chr1 42628361 42630395 uc001chd.1 chr1 42642209 42800903 uc001chf.3 chr1 42846467 42889900 uc001chi.2 chr1 42896000 42921938 uc001chj.3 chr1 42922172 42926086 uc001chl.3 chr1 43000559 43120335 uc009vwk.1 chr1 43124047 43142429 uc001chq.3 chr1 43144356 43147330 uc001chr.3 chr1 43148065 43168020 uc001chs.3 chr1 43198763 43205925 uc001cht.1 chr1 43212005 43232755 uc001chx.4 chr1 43232915 43241413 uc001cia.4 chr1 43253660 43263901 uc021omg.1 chr1 43272722 43283059 uc001cib.2 chr1 43291248 43310660 uc001cie.1 chr1 43312243 43318146 uc021omh.1 chr1 43323292 43354460 uc001cij.1 chr1 43391045 43424847 uc001cik.2 chr1 43424719 43449029 uc001cil.3 chr1 43489219 43489308 uc021omj.1 chr1 43585818 43611958 uc009vwn.1 chr1 43613593 43622067 uc009vwo.3 chr1 43629844 43638241 uc010ojx.2 chr1 43638000 43720029 uc021omk.1 chr1 43735664 43739673 uc021oml.1 chr1 43747556 43751250 uc001cit.4 chr1 43766565 43788781 uc001ciu.3 chr1 43803474 43820135 uc001ciw.3 chr1 43824625 43828873 uc001cix.3 chr1 43829067 43833409 uc031pmf.1 chr1 43849578 43855483 uc001cje.2 chr1 43855555 43919918 uc001cjk.3 chr1 43916673 43919660 uc001cjo.3 chr1 43996546 44089343 uc001cjr.3 chr1 44115796 44171189 uc001cjx.3 chr1 44165355 44173012 uc001cjy.4 chr1 44173203 44396837 uc001cjz.4 chr1 44182137 44182227 uc021oms.1 chr1 44398991 44402912 uc001ckt.3 chr1 44412477 44433694 uc001ckx.3 chr1 44435652 44439043 uc001ckz.3 chr1 44440601 44443972 uc001cld.3 chr1 44445607 44456843 uc010okl.2 chr1 44457279 44462198 uc001clj.3 chr1 44462154 44483012 uc001cll.4 chr1 44584521 44600809 uc001clp.3 chr1 44679124 44686351 uc001clq.1 chr1 44686741 44820939 uc001clt.3 chr1 44870959 45117396 uc001clv.1 chr1 45011164 45011224 uc031pmh.1 chr1 45119500 45140099 uc001cmc.3 chr1 45140393 45191263 uc001cmf.2 chr1 45205489 45233438 uc001cmg.4 chr1 45241245 45244412 uc001cmi.3 chr1 45241536 45241610 uc001cmj.1 chr1 45242163 45242261 uc001cmk.1 chr1 45243513 45243584 uc009vxi.3 chr1 45244061 45244130 uc001cml.3 chr1 45249256 45253426 uc001cmm.3 chr1 45266035 45271667 uc001cmn.3 chr1 45271585 45272957 uc001cmp.3 chr1 45274153 45279801 uc010ole.1 chr1 45287937 45308616 uc010olf.2 chr1 45316193 45452394 uc001cmt.3 chr1 45468219 45477027 uc009vxk.3 chr1 45477804 45481341 uc001cna.2 chr1 45482075 45672250 uc001cnd.2 chr1 45769581 45771291 uc010olk.2 chr1 45792544 45794346 uc001cne.3 chr1 45794913 45806142 uc009vxp.3 chr1 45805341 45809650 uc009vxq.3 chr1 45809554 45956840 uc001cns.1 chr1 45959597 45965751 uc001cnw.3 chr1 45965855 45976739 uc009vxv.3 chr1 45976706 45988562 uc021omw.1 chr1 46016454 46035723 uc001coe.3 chr1 46049659 46084578 uc001coi.2 chr1 46085715 46089731 uc010olt.2 chr1 46092975 46152302 uc001coq.3 chr1 46111451 46112357 uc010olu.1 chr1 46153846 46160108 uc001cor.1 chr1 46164406 46216485 uc001cou.3 chr1 46269284 46501796 uc001cov.3 chr1 46505811 46598380 uc001cpb.4 chr1 46640748 46651634 uc001cpd.3 chr1 46654352 46685977 uc001cpg.3 chr1 46669005 46686928 uc010oma.2 chr1 46713366 46744145 uc009vye.2 chr1 46744071 46769038 uc001cpn.3 chr1 46769379 46782447 uc001cpp.3 chr1 46805848 46830824 uc001cpr.2 chr1 46859938 46879520 uc001cpu.2 chr1 46899498 46911374 uc021ona.1 chr1 46972667 46979886 uc001cpx.3 chr1 47004367 47035927 uc021onb.1 chr1 47011315 47015678 uc001cpy.2 chr1 47011315 47016887 uc009vyh.1 chr1 47023078 47069966 uc001cqb.4 chr1 47073386 47080805 uc001cqe.4 chr1 47100710 47134099 uc001cqh.4 chr1 47137496 47139256 uc001cqj.3 chr1 47139707 47157769 uc021ond.1 chr1 47140830 47184736 uc001cqk.4 chr1 47264669 47285021 uc001cqn.4 chr1 47308766 47366147 uc031pmm.1 chr1 47394845 47407156 uc001cqp.4 chr1 47489239 47516423 uc001cqt.3 chr1 47533159 47583992 uc001cqu.1 chr1 47603106 47614526 uc001cqv.1 chr1 47644921 47646011 uc031pmn.1 chr1 47649260 47655771 uc001cqw.3 chr1 47681962 47689770 uc009vyq.2 chr1 47681962 47695443 uc001cqx.2 chr1 47691628 47691655 uc021onf.1 chr1 47715810 47779819 uc001crd.1 chr1 47799468 47844511 uc001cri.3 chr1 47859449 47861215 uc001crj.1 chr1 47881743 47883724 uc001crk.3 chr1 47897806 47900313 uc001crl.3 chr1 47901688 47906363 uc001crm.3 chr1 48226199 48462562 uc021ong.1 chr1 48567386 48648100 uc010omr.1 chr1 48688356 48714316 uc001crn.2 chr1 48761043 48937876 uc001crr.2 chr1 48998526 50489626 uc001cru.2 chr1 49193539 49242547 uc001crx.4 chr1 50574593 50667540 uc001csb.2 chr1 50883222 50889119 uc010onb.2 chr1 50906934 51425936 uc001cse.1 chr1 51048075 51048183 uc021onh.1 chr1 51435641 51440309 uc001csg.3 chr1 51567905 51613754 uc001csh.3 chr1 51701944 51739119 uc001csi.4 chr1 51752929 51810785 uc010onf.2 chr1 51819934 51984995 uc001csq.1 chr1 52004296 52004401 uc021oni.1 chr1 52082545 52254891 uc001csu.3 chr1 52254865 52344609 uc001ctc.4 chr1 52373627 52456436 uc001cth.3 chr1 52461412 52461712 uc021onk.1 chr1 52485803 52521843 uc001cti.4 chr1 52497776 52499472 uc001ctj.1 chr1 52521856 52556388 uc001ctk.3 chr1 52607765 52812358 uc001cto.4 chr1 52816264 52831877 uc001ctq.2 chr1 52838500 52870143 uc001ctu.3 chr1 52870218 52883992 uc001ctv.4 chr1 52870218 52883992 uc001ctw.4 chr1 52888947 53018762 uc001cty.2 chr1 53068042 53074723 uc001cue.3 chr1 53099065 53122737 uc001cuf.3 chr1 53152013 53164038 uc001cui.2 chr1 53192130 53293013 uc001cuj.3 chr1 53308182 53360247 uc001cuk.2 chr1 53361581 53387591 uc001cup.4 chr1 53392900 53517289 uc001cur.2 chr1 53527723 53551174 uc010onr.2 chr1 53552854 53608289 uc001cuy.3 chr1 53580247 53584281 uc001cva.1 chr1 53662100 53679869 uc001cvb.4 chr1 53679771 53686289 uc001cvd.3 chr1 53692563 53704282 uc001cvf.2 chr1 53704281 53708455 uc001cvg.3 chr1 53708040 53793821 uc001cvi.2 chr1 53793904 53802889 uc001cvn.1 chr1 53904042 53905693 uc009vzj.3 chr1 53925071 53933158 uc001cvq.1 chr1 53971905 54199877 uc001cvr.1 chr1 54231133 54304225 uc001cvs.3 chr1 54317391 54355487 uc001cvu.3 chr1 54359860 54376759 uc001cwb.3 chr1 54387233 54411288 uc001cwh.3 chr1 54411998 54433841 uc001cwj.2 chr1 54472970 54483859 uc001cwm.2 chr1 54497348 54519111 uc001cwp.3 chr1 54519273 54565416 uc001cwt.1 chr1 54519751 54519827 uc021ons.1 chr1 54604667 54618679 uc001cwv.2 chr1 54638026 54665746 uc009vzo.3 chr1 54665839 54684056 uc001cxa.4 chr1 54691103 54872068 uc001cxe.4 chr1 55013806 55076005 uc001cxl.2 chr1 55074849 55089200 uc001cxn.3 chr1 55107426 55175939 uc010ooe.1 chr1 55181494 55208328 uc001cxx.4 chr1 55222570 55230226 uc001cxy.3 chr1 55246751 55266941 uc009vzt.1 chr1 55271735 55307937 uc001cyb.4 chr1 55315299 55352921 uc001cyc.1 chr1 55352654 55353883 uc021onu.1 chr1 55423541 55423614 uc021onv.1 chr1 55446464 55457966 uc001cyd.3 chr1 55464616 55474465 uc001cye.3 chr1 55505148 55530526 uc001cyf.2 chr1 55532031 55681039 uc021onw.1 chr1 55681080 55683128 uc021onx.1 chr1 55691313 55691396 uc021ony.1 chr1 55842198 55842525 uc021onz.1 chr1 55950543 55950645 uc021ooa.1 chr1 56046709 56200675 uc001cyi.1 chr1 56960418 57045257 uc001cyj.2 chr1 57110989 57181008 uc001cyk.4 chr1 57184476 57285369 uc001cym.4 chr1 57289353 57292593 uc001cyn.3 chr1 57320442 57383894 uc001cyo.2 chr1 57394882 57431688 uc001cyp.3 chr1 57463578 58716211 uc001cys.1 chr1 58326214 58328786 uc001cyu.1 chr1 58933598 58934677 uc001cyw.1 chr1 58946390 59012446 uc001cyy.3 chr1 59041094 59043166 uc001cyz.4 chr1 59120410 59165747 uc009wab.2 chr1 59246462 59249785 uc001cze.3 chr1 59250822 59365384 uc010oop.1 chr1 59597607 59612479 uc010ooq.2 chr1 59762624 60228402 uc009wac.3 chr1 60198898 60198968 uc021oob.1 chr1 60238466 60254501 uc001czn.3 chr1 60280532 60342050 uc001czo.3 chr1 60358979 60392423 uc001czq.3 chr1 60454823 60539442 uc001czs.2 chr1 61125302 61291256 uc001czt.1 chr1 61405915 61436448 uc001czu.3 chr1 61547533 61928460 uc010oos.2 chr1 62119913 62121800 uc031pmt.1 chr1 62146718 62191095 uc001czz.1 chr1 62208148 62629591 uc001dab.3 chr1 62318170 62318274 uc031pmu.1 chr1 62660473 62678001 uc001dae.4 chr1 62701836 62785083 uc001dah.4 chr1 62901974 62917475 uc001dak.2 chr1 62920396 63154039 uc001daq.4 chr1 63063157 63071976 uc001das.2 chr1 63249776 63330941 uc001dau.3 chr1 63624753 63782901 uc001daw.2 chr1 63704613 63704845 uc021ood.1 chr1 63788729 63790797 uc001dax.2 chr1 63799429 63799491 uc021ooe.1 chr1 63833260 63904233 uc021oof.1 chr1 63906440 63988944 uc001dbb.2 chr1 63989012 64038364 uc001dbf.3 chr1 64014650 64016307 uc001dbg.1 chr1 64088886 64125916 uc010ooz.2 chr1 64239689 64647179 uc001dbj.3 chr1 64262024 64262128 uc021oog.1 chr1 64571005 64636980 uc001dbl.3 chr1 64669489 64710027 uc001dbn.1 chr1 64936475 65158741 uc001dbo.1 chr1 65045529 65045604 uc021ooh.1 chr1 65210777 65298914 uc001dbs.2 chr1 65298905 65432187 uc001dbu.1 chr1 65445259 65468159 uc001dbw.3 chr1 65488650 65488757 uc021ooj.1 chr1 65523437 65523525 uc021ook.1 chr1 65524116 65524191 uc001dbx.3 chr1 65613231 65697828 uc001dby.3 chr1 65775217 65881552 uc001dce.2 chr1 65886130 66103176 uc001dci.3 chr1 65886130 65901690 uc001dcf.3 chr1 66258855 66840262 uc001dco.3 chr1 66560143 66560229 uc021oom.1 chr1 66999824 67210768 uc001dcr.3 chr1 67094122 67094200 uc021oon.1 chr1 67132271 67142710 uc010ope.1 chr1 67218139 67244730 uc001dcv.3 chr1 67263423 67266942 uc001dcw.3 chr1 67278571 67390570 uc001dcx.3 chr1 67390577 67454302 uc001dde.2 chr1 67465014 67520080 uc001ddk.2 chr1 67557858 67600654 uc001ddm.2 chr1 67632168 67725650 uc001ddo.3 chr1 67661822 67661926 uc021ooo.1 chr1 67773046 67862583 uc001ddu.3 chr1 67873492 67896123 uc001ddv.3 chr1 68150859 68154021 uc001ddz.2 chr1 68167148 68299155 uc001dea.2 chr1 68238275 68238336 uc021oop.1 chr1 68297970 68668670 uc001deb.2 chr1 68511644 68516481 uc001ded.3 chr1 68564141 68698284 uc001dee.3 chr1 68649200 68649293 uc021oos.1 chr1 68649301 68649321 uc021oot.1 chr1 68894506 68915642 uc001dei.1 chr1 68939834 68962799 uc001dem.4 chr1 68962358 69004310 uc001den.3 chr1 70225857 70589171 uc001dep.3 chr1 70385004 70386000 uc009wbh.2 chr1 70610484 70671361 uc001der.2 chr1 70671364 70717701 uc001des.3 chr1 70724684 70820417 uc001dex.4 chr1 70820492 70833705 uc001dfa.3 chr1 70876900 70905534 uc001dfd.3 chr1 71172135 71252151 uc001dff.3 chr1 71418114 71513491 uc001dfo.3 chr1 71512188 71532865 uc001dfr.3 chr1 71528973 71546972 uc001dft.3 chr1 71533313 71533399 uc010oqr.1 chr1 71547006 71703406 uc001dfu.2 chr1 71868624 72748405 uc001dfw.3 chr1 72259914 72302695 uc031pmw.1 chr1 73771852 73804560 uc001dfx.3 chr1 74491701 74663871 uc001dfy.4 chr1 74663895 75010116 uc001dge.2 chr1 75033794 75139422 uc001dgg.3 chr1 75043113 75091782 uc001dgh.3 chr1 75171171 75199092 uc001dgj.3 chr1 75198835 75232360 uc001dgn.3 chr1 75595658 75598261 uc001dgp.1 chr1 75600566 75627218 uc031pmx.1 chr1 75672074 76076799 uc001dgu.3 chr1 76103850 76188721 uc001dgv.3 chr1 76190042 76229355 uc009wbp.3 chr1 76251878 76260775 uc001dgy.2 chr1 76252756 76252834 uc001dgz.2 chr1 76253573 76253657 uc009wbu.1 chr1 76255161 76255232 uc009wbv.1 chr1 76262555 76378923 uc001dhd.2 chr1 76384557 76398116 uc001dhe.2 chr1 76540388 77096669 uc001dhh.2 chr1 77333185 77529737 uc001dhi.3 chr1 77554666 77685132 uc001dhk.3 chr1 77747661 78025654 uc001dhn.3 chr1 78030189 78148343 uc001dhq.3 chr1 78161673 78225564 uc001dht.4 chr1 78245308 78345225 uc010ork.3 chr1 78354199 78409578 uc001dic.4 chr1 78413590 78444777 uc001dii.3 chr1 78470635 78482995 uc001dij.3 chr1 78511588 78603112 uc001dik.3 chr1 78560490 78560599 uc021oov.1 chr1 78695282 78759574 uc001dil.1 chr1 78956727 79006386 uc001din.3 chr1 79086087 79111830 uc010oro.2 chr1 79115476 79129763 uc001dip.4 chr1 79152744 79152828 uc021oow.1 chr1 79355448 79472495 uc001diq.4 chr1 82266081 82458107 uc001diu.3 chr1 82312940 82313045 uc021oox.1 chr1 83439565 83451891 uc001dix.4 chr1 83911736 83920454 uc001diy.3 chr1 84041470 84326679 uc001diz.4 chr1 84259583 84259634 uc021ooy.1 chr1 84259597 84379059 uc031pmy.1 chr1 84267442 84326229 uc001dja.1 chr1 84335056 84464833 uc001djc.3 chr1 84379036 84379062 uc021ooz.1 chr1 84609951 84704181 uc001djl.3 chr1 84743003 84743140 uc021opa.1 chr1 84764048 84816481 uc001djr.3 chr1 84810360 84816481 uc001djs.3 chr1 84830640 84863576 uc009wcg.3 chr1 84864214 84880691 uc001djt.1 chr1 84944919 84964033 uc001djv.4 chr1 84964005 84972262 uc001djw.4 chr1 84972012 85014647 uc021opb.1 chr1 85018803 85040163 uc001dka.2 chr1 85093912 85097429 uc010ory.1 chr1 85109389 85156240 uc001dkj.3 chr1 85279085 85358896 uc009wcj.1 chr1 85391265 85462796 uc001dkm.3 chr1 85483764 85514223 uc001dkp.3 chr1 85527992 85598821 uc001dkt.3 chr1 85599476 85599556 uc021opc.1 chr1 85623355 85666728 uc009wcm.3 chr1 85715636 85725355 uc001dkv.3 chr1 85731459 85742587 uc021opd.1 chr1 85742040 85865646 uc001dla.2 chr1 85784167 85930889 uc001dlb.3 chr1 86046443 86049648 uc001dle.3 chr1 86115105 86174116 uc001dlh.3 chr1 86194915 86622154 uc001dlj.3 chr1 86815776 86862025 uc001dll.2 chr1 86889768 86922240 uc001dlr.4 chr1 86934394 86965974 uc001dlt.3 chr1 87012758 87046432 uc009wcs.3 chr1 87099958 87121059 uc010osh.2 chr1 87170252 87213867 uc001dly.3 chr1 87328127 87380107 uc021opi.1 chr1 87380334 87575681 uc010osk.2 chr1 87597679 87598718 uc021opj.1 chr1 87777576 87777676 uc021opk.1 chr1 87794150 87814607 uc001dmi.3 chr1 87819209 87837338 uc001dmk.3 chr1 87918922 87919056 uc021opl.1 chr1 88943510 88943810 uc021opm.1 chr1 89004735 89150887 uc021opn.1 chr1 89149921 89301938 uc001dmn.3 chr1 89318320 89357301 uc001dmo.4 chr1 89401455 89458643 uc001dmp.2 chr1 89445138 89458643 uc001dms.3 chr1 89472359 89488549 uc001dmt.3 chr1 89517986 89531043 uc001dmx.2 chr1 89573309 89591799 uc001dmz.1 chr1 89597433 89641723 uc001dna.2 chr1 89646830 89664633 uc001dnb.3 chr1 89724633 89738544 uc001dnd.3 chr1 89754940 89756045 uc031pna.1 chr1 89829435 89853719 uc001dnf.2 chr1 89873237 89890493 uc009wcy.1 chr1 89990396 90063420 uc001dni.3 chr1 90090407 90098453 uc001dnk.1 chr1 90098643 90185094 uc001dnl.4 chr1 90287479 90401989 uc001dnn.3 chr1 90453271 90453503 uc021opr.1 chr1 90458823 90460525 uc001dno.3 chr1 90460677 90494094 uc001dnq.2 chr1 91177578 91182794 uc001dns.3 chr1 91295103 91317175 uc001dnu.2 chr1 91380856 91487812 uc001dnw.3 chr1 91726322 91870426 uc001doa.4 chr1 91966403 91991321 uc001dof.3 chr1 92145899 92351836 uc001doh.3 chr1 92295329 92295626 uc021ops.1 chr1 92414927 92479985 uc010osz.2 chr1 92495532 92529093 uc001don.2 chr1 92545861 92613401 uc001doo.3 chr1 92632608 92650280 uc010otd.2 chr1 92683572 92711367 uc001doq.3 chr1 92711954 92764566 uc001dor.3 chr1 92764521 92853732 uc001dot.2 chr1 92940317 92951628 uc001dov.4 chr1 92974252 93257961 uc001dox.3 chr1 93297593 93307481 uc001doz.3 chr1 93302845 93302940 uc001dpe.2 chr1 93303574 93303627 uc021opt.1 chr1 93307716 93427079 uc001dpg.3 chr1 93544791 93604638 uc009wdj.3 chr1 93615298 93646246 uc001dpn.3 chr1 93646280 93744287 uc021opx.1 chr1 93775665 93811368 uc001dpt.2 chr1 93811477 93828148 uc001dpu.3 chr1 93913687 94020218 uc010otk.2 chr1 93981833 93981906 uc021opz.1 chr1 94027342 94146926 uc001dpz.4 chr1 94057524 94065587 uc009wdn.3 chr1 94219111 94240930 uc001dqd.1 chr1 94312387 94312467 uc021oqa.1 chr1 94313128 94313213 uc021oqb.1 chr1 94317792 94319887 uc001dqe.1 chr1 94335013 94344762 uc001dqf.3 chr1 94352589 94375012 uc001dqg.1 chr1 94458393 94586705 uc001dqh.3 chr1 94634462 94703307 uc001dqj.4 chr1 94883932 94984219 uc001dqn.4 chr1 94994731 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225615815 uc001hoy.3 chr1 225674533 225840845 uc001hpc.1 chr1 225888305 225939945 uc001hpe.1 chr1 225965514 225978168 uc001hpg.3 chr1 225997796 226033262 uc001hpk.3 chr1 226033232 226070420 uc001hpm.2 chr1 226073981 226076846 uc001hpo.3 chr1 226107576 226112040 uc001hpq.4 chr1 226124297 226129083 uc001hpt.2 chr1 226170402 226187066 uc001hpu.4 chr1 226250407 226259703 uc001hpw.3 chr1 226271655 226277994 uc001hpx.3 chr1 226332379 226374423 uc001hpy.3 chr1 226374532 226385086 uc031psl.1 chr1 226411382 226413513 uc010pvm.2 chr1 226418849 226497204 uc001hqa.3 chr1 226548391 226595801 uc001hqd.4 chr1 226723318 226730469 uc001hqe.2 chr1 226736500 226796915 uc021pjw.1 chr1 226819390 226926876 uc010pvo.2 chr1 227058272 227083804 uc009xeo.1 chr1 227127937 227175246 uc001hqn.1 chr1 227177565 227505826 uc001hqr.3 chr1 227581291 227618723 uc001hqv.3 chr1 227751219 227850164 uc021pjy.1 chr1 227884732 227885408 uc021pjz.1 chr1 227918889 227923112 uc001hrb.3 chr1 227922696 227968932 uc001hrf.2 chr1 228003417 228034171 uc001hrh.3 chr1 228109164 228135676 uc001hri.2 chr1 228129290 228129384 uc031psm.1 chr1 228155293 228155325 uc001hrj.3 chr1 228155355 228155417 uc001hrk.3 chr1 228156485 228156575 uc021pka.1 chr1 228156672 228156704 uc021pkb.1 chr1 228157022 228157062 uc010pvt.1 chr1 228157172 228157203 uc010pvu.2 chr1 228160064 228160098 uc001hrn.2 chr1 228160168 228160213 uc021pkc.1 chr1 228194722 228248972 uc001hrq.2 chr1 228270360 228286913 uc001hrr.3 chr1 228284963 228285042 uc021pkd.1 chr1 228288427 228291022 uc001hrx.3 chr1 228294379 228297013 uc001hrz.4 chr1 228327928 228336655 uc021pkf.1 chr1 228337414 228347527 uc001hsk.3 chr1 228353428 228369958 uc001hsl.4 chr1 228391206 228401365 uc031psn.1 chr1 228395830 228566575 uc001hsq.2 chr1 228581376 228594517 uc001hss.3 chr1 228595635 228604583 uc001hsv.3 chr1 228612545 228613026 uc001hsx.1 chr1 228645064 228645560 uc001hsy.3 chr1 228645807 228646259 uc001hsz.3 chr1 228649774 228649853 uc021pkh.1 chr1 228651845 228651891 uc021pki.1 chr1 228673888 228674821 uc001hta.3 chr1 228675067 228683889 uc001htb.3 chr1 228780393 228788159 uc001htd.2 chr1 228870823 228882416 uc001htf.3 chr1 229050379 229052954 uc001htg.2 chr1 229406808 229441640 uc001hth.4 chr1 229440128 229441250 uc001htk.4 chr1 229456751 229478688 uc001htl.4 chr1 229566992 229569843 uc001htm.3 chr1 229577043 229644088 uc001htn.3 chr1 229589676 229591617 uc001hto.1 chr1 229652328 229694442 uc001htp.4 chr1 229728865 229761794 uc001htq.3 chr1 229761980 229795946 uc001hts.1 chr1 230202955 230417875 uc010pwa.1 chr1 230457391 230561674 uc031psq.1 chr1 230778201 230829731 uc001htw.3 chr1 230838271 230850336 uc001hty.4 chr1 230883129 230937749 uc001htz.1 chr1 230972864 231004302 uc001hub.3 chr1 231010591 231014761 uc001hue.1 chr1 231041986 231114618 uc001huf.4 chr1 231114822 231136479 uc001huh.3 chr1 231154703 231175995 uc001hui.2 chr1 231298673 231357314 uc009xfn.1 chr1 231319844 231323373 uc031pss.1 chr1 231359508 231376924 uc001hul.3 chr1 231376918 231413719 uc001hup.4 chr1 231468481 231473578 uc001huq.3 chr1 231473681 231490769 uc001hur.4 chr1 231499496 231560790 uc001huv.2 chr1 231611509 231612271 uc021pkl.1 chr1 231664398 231702269 uc001huw.3 chr1 231727037 231747836 uc021pkm.2 chr1 231762560 232177019 uc010pxh.2 chr1 231950371 231954263 uc001hvd.3 chr1 232533711 232651243 uc001hvg.3 chr1 232940637 232946092 uc001hvh.2 chr1 233086369 233114219 uc001hvj.1 chr1 233119881 233431459 uc001hvl.2 chr1 233463513 233520894 uc001hvt.4 chr1 233584371 233584527 uc021pko.1 chr1 233749749 233808258 uc010pxo.1 chr1 233759897 233759965 uc021pkp.1 chr1 234040678 234460262 uc001hvy.1 chr1 234348351 234350834 uc001hvz.1 chr1 234442212 234442285 uc021pkq.1 chr1 234509428 234519795 uc001hwc.3 chr1 234527058 234614849 uc001hwd.3 chr1 234663636 234667525 uc001hwe.3 chr1 234740014 234745271 uc001hwg.3 chr1 234765056 234770526 uc021pkr.1 chr1 234782034 234796670 uc001hwh.3 chr1 234859788 234867390 uc001hwj.2 chr1 235093089 235099746 uc001hwk.4 chr1 235272657 235292256 uc001hwl.3 chr1 235291117 235291252 uc001hwm.1 chr1 235294497 235324772 uc001hwn.3 chr1 235330209 235491532 uc001hwq.3 chr1 235353348 235353431 uc021pkt.1 chr1 235491752 235507844 uc001hwv.3 chr1 235530727 235612280 uc001hxa.1 chr1 235610504 235667781 uc001hxc.3 chr1 235710984 235814054 uc001hxh.4 chr1 235712454 235714587 uc021pku.1 chr1 235824330 236030227 uc001hxj.3 chr1 236016299 236016360 uc021pkv.1 chr1 236139131 236228481 uc001hxo.3 chr1 236227659 236229779 uc001hxp.1 chr1 236305831 236372209 uc001hxq.3 chr1 236378421 236445339 uc001hxt.3 chr1 236557679 236648008 uc001hxu.1 chr1 236686368 236687808 uc001hxx.3 chr1 236686738 236716279 uc001hxy.2 chr1 236712304 236767841 uc001hyd.2 chr1 236849769 236927558 uc001hyf.2 chr1 236958580 237067281 uc001hyi.4 chr1 236973802 236992568 uc009xgj.1 chr1 237167402 237167718 uc001hyk.2 chr1 237205701 237997288 uc001hyl.1 chr1 237284106 237284409 uc021pkw.1 chr1 238025474 238091619 uc010pyc.2 chr1 238041163 238054222 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244210619 uc001iac.3 chr1 244212240 244220780 uc031psv.1 chr1 244515936 244552388 uc001iah.4 chr1 244571793 244615436 uc001iaj.3 chr1 244624672 244803662 uc001iam.3 chr1 244816351 244872334 uc001iao.3 chr1 244998638 245008359 uc001iar.3 chr1 245003939 245010243 uc001iav.3 chr1 245013601 245027827 uc001iaz.1 chr1 245133170 245251148 uc001ibc.2 chr1 245318286 245866428 uc001ibf.1 chr1 245533635 245533719 uc021plw.1 chr1 245912641 246670644 uc001ibl.3 chr1 246679340 246687589 uc021plx.1 chr1 246703862 246729565 uc001ibn.3 chr1 246729638 246831884 uc001ibp.3 chr1 246887377 246931440 uc001ibr.3 chr1 246939314 246955685 uc001ibs.1 chr1 246970416 246970487 uc021ply.1 chr1 247002401 247094726 uc001ibv.2 chr1 247148624 247171395 uc009xgu.3 chr1 247197939 247242115 uc001icd.2 chr1 247263263 247267674 uc001ice.2 chr1 247273461 247275719 uc001icg.1 chr1 247319202 247335318 uc001icj.1 chr1 247337903 247338870 uc001icl.1 chr1 247365268 247365362 uc021pma.1 chr1 247419373 247420447 uc010pyu.2 chr1 247463621 247495045 uc001ico.3 chr1 247579457 247612406 uc001icr.3 chr1 247614330 247615284 uc010pyx.2 chr1 247654369 247655711 uc001icz.2 chr1 247681594 247683942 uc001idc.3 chr1 247693433 247697141 uc009xgy.3 chr1 247712346 247739848 uc001idf.3 chr1 247751661 247752615 uc010pyy.2 chr1 247768887 247769817 uc010pyz.2 chr1 247835419 247836343 uc001idi.1 chr1 247875130 247876057 uc001idj.1 chr1 247920763 247921708 uc010pza.2 chr1 247978101 247979031 uc001idm.1 chr1 248004229 248005198 uc001idn.1 chr1 248020500 248043438 uc001ido.3 chr1 248058888 248059833 uc010pzb.2 chr1 248084319 248085258 uc010pzc.2 chr1 248100492 248264224 uc001ids.3 chr1 248112159 248113098 uc001idt.1 chr1 248128633 248129641 uc010pzd.2 chr1 248153568 248154493 uc001idv.1 chr1 248185249 248186188 uc031psy.1 chr1 248201473 248202607 uc001idw.3 chr1 248223983 248224922 uc001idx.1 chr1 248285437 248286082 uc001idy.1 chr1 248308449 248309388 uc010pze.2 chr1 248343287 248344331 uc010pzf.2 chr1 248366369 248367308 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249213345 uc001ifh.3 ================================================ FILE: intro-to-awk/data/enhancers.csv ================================================ chr1,1015066,1015266,HSE897,9.5706324138 chr1,1590473,1590673,HSE853,17.3206898329 chr1,2120861,2121064,HSE86,66.0424471614 chr1,6336418,6336624,HSE394,14.8892086906 chr1,7404594,7404794,HSE315,24.228051023 chr1,11941325,11941525,HSE322,17.5192630328 chr1,15055555,15055755,HSE962,11.7065788965 chr1,15478024,15478224,HSE354,17.5771586196 chr1,16065307,16065508,HSE264,23.9092446094 chr1,23244249,23244449,HSE434,17.1331422162 chr1,24621426,24621626,HSE638,11.9517576968 chr1,24710189,24710389,HSE537,14.6283754801 chr1,26216838,26217042,HSE237,39.9988740347 chr1,28931703,28931903,HSE150,37.3158468645 chr1,29139636,29139836,HSE696,9.3933913885 chr1,29301011,29301211,HSE837,15.2711109524 chr1,30644209,30644410,HSE452,13.1331424716 chr1,37323436,37323636,HSE144,24.6337166989 chr1,39114092,39114292,HSE642,13.5250552809 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chr12,67180805,67181005,HSE302,18.0232362712 chr12,67820036,67820236,HSE693,28.0050105025 chr12,67824724,67824924,HSE562,19.9790499282 chr12,70590594,70590798,HSE938,10.4065399522 chr12,72665258,72665458,HSE870,9.4908568562 chr12,72843735,72843935,HSE352,14.6912721831 chr12,72903763,72903963,HSE622,12.4776159762 chr12,76258547,76258747,HSE366,15.4992304899 chr12,81139497,81139697,HSE263,19.1724365769 chr12,83358298,83358498,HSE956,10.9748220911 chr12,88826891,88827091,HSE470,14.4491790975 chr12,93693111,93693311,HSE337,17.5773205057 chr12,93793026,93793226,HSE231,36.7866454534 chr12,93964730,93964930,HSE701,14.9318726929 chr12,94680437,94680638,HSE800,10.1294106105 chr12,95448207,95448407,HSE85,41.328100082 chr12,98833530,98833731,HSE572,21.9773288843 chr12,102047388,102047588,HSE177,36.6536035992 chr12,105121890,105122090,HSE733,12.9059778339 chr12,106581316,106581516,HSE974,11.9119885347 chr12,108780634,108780834,HSE195,21.1967998006 chr12,110145174,110145374,HSE326,19.9633772792 chr12,110147281,110147481,HSE269,19.4570397688 chr12,116307853,116308063,HSE340,22.419418574 chr12,117846190,117846392,HSE393,15.5403001234 chr12,117850284,117850484,HSE557,16.5566891443 chr12,123543660,123543860,HSE861,10.4781563732 chr12,128314695,128314895,HSE528,13.3821736799 chr12,128455126,128455326,HSE906,9.3701763362 chr12,131041357,131041557,HSE827,8.9137558728 chr12,131261218,131261419,HSE39,40.7349907933 chr12,131596799,131596999,HSE61,38.7314139499 chr12,131622505,131622705,HSE735,9.6402688286 chr12,133175461,133175661,HSE560,17.0748830696 chr13,21347729,21347929,HSE450,18.700409108 chr13,24148647,24148847,HSE923,12.1565857536 chr13,29459319,29459519,HSE4,83.6939521234 chr13,29464970,29465170,HSE235,21.5042630726 chr13,33536518,33536718,HSE484,11.9616526488 chr13,36045055,36045255,HSE466,15.1339680818 chr13,37089667,37089867,HSE53,34.1508321281 chr13,38195831,38196031,HSE63,29.4908349793 chr13,47090817,47091017,HSE240,20.9194834678 chr13,47169753,47169953,HSE795,10.6129192636 chr13,48685195,48685395,HSE383,17.4161328951 chr13,52195812,52196012,HSE804,33.9458114683 chr13,52928037,52928237,HSE971,10.2210243551 chr13,58387875,58388075,HSE898,9.7709738074 chr13,63964002,63964202,HSE155,21.8968073472 chr13,63982654,63982855,HSE755,10.6894623919 chr13,66918963,66919163,HSE942,8.137382847 chr13,78382044,78382244,HSE709,13.2425052878 chr13,79968085,79968285,HSE207,19.0520301367 chr13,81969573,81969773,HSE464,17.1316537183 chr13,88325009,88325225,HSE169,22.404724465 chr13,94551722,94551922,HSE47,39.9300964523 chr13,96720972,96721172,HSE242,17.6289298545 chr13,99193496,99193696,HSE775,11.8286452332 chr13,102857806,102858006,HSE175,26.1349566103 chr13,103659347,103659547,HSE879,9.4672756235 chr13,106860061,106860261,HSE523,15.2219187287 chr13,107025740,107025940,HSE545,22.9167462321 chr13,107144094,107144294,HSE826,10.0932827937 chr13,111223922,111224122,HSE55,37.6150776113 chr14,21439340,21439545,HSE697,14.9749639521 chr14,24505425,24505632,HSE598,21.3172605404 chr14,25814644,25814844,HSE884,8.4198607932 chr14,26543395,26543598,HSE239,19.7243319678 chr14,31281660,31281860,HSE64,42.0539122341 chr14,33531812,33532012,HSE374,13.6641312978 chr14,59129293,59129493,HSE803,12.357054467 chr14,59386085,59386285,HSE932,9.6556661977 chr14,61083031,61083231,HSE780,12.1130184536 chr14,65539073,65539273,HSE324,18.8187496116 chr14,76804206,76804406,HSE129,28.6611225834 chr14,81636859,81637059,HSE65,73.8674420954 chr14,81790696,81790896,HSE209,42.4669581462 chr14,91091974,91092174,HSE480,17.813206233 chr14,91716580,91716780,HSE469,17.2797598596 chr14,93949158,93949358,HSE746,9.1621125053 chr14,95071723,95071923,HSE924,8.9302006066 chr14,102783279,102783479,HSE816,24.4849615987 chr14,105147964,105148164,HSE939,11.1238681848 chr15,27524028,27524228,HSE431,15.0234209239 chr15,28817788,28817988,HSE191,21.3327480149 chr15,29247425,29247625,HSE279,18.2157833949 chr15,30182592,30182792,HSE840,10.1016943223 chr15,31792516,31792716,HSE429,15.2456002355 chr15,33752266,33752466,HSE806,7.7973232785 chr15,33958947,33959147,HSE650,11.2642323912 chr15,39474262,39474462,HSE703,12.786143498 chr15,52554008,52554208,HSE832,10.0325076389 chr15,54967526,54967726,HSE230,24.1378839612 chr15,55510355,55510555,HSE339,18.1931930527 chr15,61269359,61269560,HSE1,89.2659435972 chr15,61979890,61980090,HSE922,8.1022018494 chr15,62752114,62752314,HSE611,14.5613676786 chr15,62917755,62917955,HSE305,28.6662298913 chr15,68723591,68723791,HSE462,23.2304619822 chr15,69830362,69830562,HSE757,10.8328824475 chr15,72203345,72203545,HSE147,31.2986371967 chr15,74076783,74076983,HSE771,12.0826546194 chr15,74596170,74596370,HSE993,12.6758156277 chr15,74641836,74642036,HSE221,25.8164928998 chr15,75523071,75523271,HSE874,9.5089830845 chr15,80478573,80478773,HSE211,20.487080964 chr15,80828906,80829107,HSE277,17.6534731911 chr15,81616460,81616661,HSE101,37.8792330626 chr15,81861640,81861840,HSE987,12.411938064 chr15,81868202,81868403,HSE587,14.0269438152 chr15,82201705,82201905,HSE376,27.8796005059 chr15,86374626,86374826,HSE588,14.9950787059 chr15,88418444,88418645,HSE580,12.4183759864 chr15,88670015,88670215,HSE521,11.1843930949 chr15,92692436,92692637,HSE26,72.0593711439 chr15,98775365,98775565,HSE381,14.9440294085 chr15,102501629,102501829,HSE592,17.8647492194 chr16,626697,626897,HSE208,23.3415017382 chr16,970811,971011,HSE408,18.879805744 chr16,3137379,3137579,HSE656,13.1191220261 chr16,11114848,11115048,HSE998,11.8718870193 chr16,11173789,11173989,HSE128,39.6118207734 chr16,19756698,19756898,HSE349,18.1890890859 chr16,22776292,22776492,HSE631,10.2281468082 chr16,23037379,23037579,HSE661,11.5338091882 chr16,24508521,24508721,HSE731,10.9292976321 chr16,27323049,27323272,HSE62,34.6319360162 chr16,30204374,30204574,HSE426,34.9445834473 chr16,31885117,31885317,HSE805,17.0726534038 chr16,48565689,48565889,HSE914,14.4461848582 chr16,52484957,52485157,HSE999,9.9367824367 chr16,56581581,56581781,HSE386,14.3620121795 chr16,59736519,59736719,HSE342,19.0406902327 chr16,66545956,66546156,HSE905,9.2931748668 chr16,68484705,68484905,HSE218,20.7512770405 chr16,68507748,68507957,HSE548,18.0413570871 chr16,73322142,73322343,HSE702,12.5680470461 chr16,78355884,78356084,HSE303,20.3741349926 chr16,89307265,89307465,HSE18,70.9382862202 chr16,89679724,89679939,HSE578,17.1619789211 chr16,89988292,89988493,HSE347,20.0128724894 chr17,999331,999532,HSE482,15.5034626446 chr17,5579439,5579642,HSE48,43.9178228518 chr17,6111243,6111443,HSE198,30.4698391934 chr17,8279924,8280124,HSE45,65.9896646322 chr17,8759325,8759525,HSE422,15.0545562099 chr17,9340019,9340219,HSE948,9.8219471135 chr17,11166409,11166610,HSE122,22.963059202 chr17,12830908,12831108,HSE145,27.0976894901 chr17,13298874,13299074,HSE156,34.5395597863 chr17,17002215,17002415,HSE654,16.2936251743 chr17,17302370,17302570,HSE372,29.9282249338 chr17,21030152,21030352,HSE903,14.116644535 chr17,21367528,21367728,HSE833,9.7516907593 chr17,30088186,30088386,HSE678,13.3589311568 chr17,33774498,33774698,HSE818,9.4607428819 chr17,34541456,34541656,HSE553,11.9696962671 chr17,42385237,42385437,HSE149,30.3729032338 chr17,43419034,43419235,HSE437,27.2450803684 chr17,45681996,45682196,HSE438,15.031012053 chr17,49008988,49009188,HSE538,15.8789561003 chr17,49433023,49433224,HSE11,58.5889197596 chr17,49687897,49688097,HSE413,16.1529684506 chr17,49807832,49808032,HSE2,84.3204244342 chr17,50624462,50624662,HSE20,42.9975194309 chr17,50767466,50767666,HSE258,24.1676282047 chr17,53412056,53412256,HSE243,26.149501739 chr17,53828393,53828601,HSE617,14.5154098498 chr17,55785272,55785472,HSE892,10.3430074886 chr17,57998741,57998941,HSE978,10.1861279752 chr17,59327373,59327573,HSE774,9.3428992552 chr17,59589126,59589326,HSE529,18.5934605421 chr17,62254441,62254641,HSE133,33.7747197413 chr17,64370576,64370776,HSE964,7.825535696 chr17,72113540,72113740,HSE607,21.5911019025 chr17,72967433,72967633,HSE377,16.2493907656 chr17,73966346,73966547,HSE881,9.5338664732 chr17,75125788,75125988,HSE407,21.4349393235 chr17,75279445,75279646,HSE527,11.5655867334 chr17,79050003,79050203,HSE445,15.299617834 chr17,80189664,80189864,HSE957,8.575676987 chr18,2847905,2848105,HSE680,16.1823388257 chr18,3297314,3297514,HSE51,55.4969408846 chr18,4107882,4108082,HSE756,10.6509843928 chr18,4455211,4455413,HSE173,26.658560462 chr18,5960317,5960517,HSE404,17.5337872123 chr18,6610590,6610790,HSE5,67.4765594962 chr18,6878970,6879170,HSE21,114.4591803017 chr18,6941554,6941754,HSE925,8.8831899962 chr18,8067140,8067340,HSE515,15.8060479397 chr18,8982773,8982973,HSE674,10.8336659886 chr18,8991089,8991289,HSE871,9.2212572719 chr18,11092726,11092926,HSE603,14.5685707705 chr18,20949254,20949454,HSE940,10.1089249342 chr18,22006557,22006757,HSE575,21.5810391792 chr18,24341571,24341772,HSE865,12.220860758 chr18,30186665,30186865,HSE96,29.1942230497 chr18,33597390,33597591,HSE935,14.2979081707 chr18,38116709,38116909,HSE94,27.5166517092 chr18,38614509,38614709,HSE516,22.9528532124 chr18,41456364,41456564,HSE483,22.2977384345 chr18,43802028,43802228,HSE508,12.8490635247 chr18,46386532,46386733,HSE676,14.0371357841 chr18,47441756,47441956,HSE282,17.402175324 chr18,48344045,48344245,HSE640,26.5533837237 chr18,57865414,57865614,HSE637,12.1799986999 chr18,60810573,60810773,HSE712,10.7941794086 chr18,66106367,66106567,HSE78,34.0539230971 chr18,67067626,67067826,HSE748,20.3756153543 chr18,67206189,67206389,HSE707,14.8194102675 chr18,77862401,77862601,HSE778,8.9773592552 chr19,1855466,1855666,HSE615,21.0205633353 chr19,7864445,7864654,HSE420,22.2334775436 chr19,7936518,7936718,HSE1000,9.890269251 chr19,14134468,14134668,HSE552,19.247739132 chr19,16568459,16568659,HSE690,12.8248560895 chr19,22053766,22053966,HSE325,15.1569835215 chr19,28284760,28284962,HSE35,44.2571129827 chr19,28609278,28609478,HSE262,25.334253518 chr19,28660492,28660692,HSE644,23.3221045263 chr19,30811887,30812087,HSE120,27.7702458019 chr19,35800455,35800659,HSE498,13.5519561083 chr19,36792015,36792215,HSE953,7.5220582555 chr19,39122462,39122662,HSE582,17.8131880246 chr19,39167150,39167350,HSE395,15.4118441749 chr19,42905988,42906189,HSE934,12.7825653114 chr19,43059635,43059838,HSE535,11.755421727 chr19,44711537,44711737,HSE798,18.193542685 chr19,50175593,50175793,HSE781,13.7950661987 chr19,51289242,51289442,HSE917,9.3858171773 chr19,51900838,51901038,HSE453,18.6017463436 chr19,53039209,53039409,HSE463,19.5962420571 chr2,4564065,4564265,HSE689,11.0832062757 chr2,9136176,9136376,HSE166,25.108740724 chr2,11761490,11761690,HSE955,9.1404770745 chr2,14359777,14359977,HSE729,9.1222131108 chr2,15208167,15208367,HSE751,11.003441743 chr2,17699479,17699679,HSE759,12.4462128012 chr2,17721672,17721872,HSE742,14.1970571736 chr2,20024086,20024286,HSE83,28.7067393754 chr2,23725997,23726197,HSE449,24.6822465458 chr2,25752130,25752330,HSE346,16.6877757619 chr2,29434845,29435045,HSE841,9.2654187933 chr2,29840944,29841144,HSE785,11.1516309255 chr2,33308933,33309133,HSE810,10.6180361689 chr2,40274443,40274643,HSE543,16.5775325601 chr2,40277040,40277240,HSE901,17.7522146515 chr2,43020786,43020986,HSE536,13.7021711702 chr2,43525771,43525971,HSE140,23.6931498342 chr2,45907824,45908024,HSE790,12.332179446 chr2,49482822,49483023,HSE764,11.6109483119 chr2,49776850,49777050,HSE382,20.4425087695 chr2,49829759,49829959,HSE559,13.8791198577 chr2,50199950,50200150,HSE306,21.1830037687 chr2,50522255,50522455,HSE294,20.4771502209 chr2,50573272,50573472,HSE520,11.3849059039 chr2,53710769,53710969,HSE673,11.4462899835 chr2,54846090,54846291,HSE652,12.3643734532 chr2,55381344,55381548,HSE333,16.5384641969 chr2,55649064,55649265,HSE161,26.4627203219 chr2,65290575,65290775,HSE860,14.2637154595 chr2,65615621,65615821,HSE811,10.640516431 chr2,68546802,68547002,HSE585,12.806795592 chr2,72975882,72976082,HSE506,11.8250162965 chr2,74234353,74234554,HSE447,19.3111885875 chr2,76692583,76692783,HSE308,24.5495576527 chr2,84914260,84914460,HSE273,18.7931730309 chr2,85289844,85290044,HSE490,17.6255300394 chr2,85378807,85379007,HSE442,18.4954416724 chr2,87051461,87051665,HSE139,28.4199186304 chr2,87064810,87065010,HSE708,10.458886264 chr2,87067122,87067449,HSE379,30.0280259634 chr2,87069310,87069510,HSE885,16.1725172352 chr2,88316314,88316515,HSE214,34.3179189629 chr2,88414573,88414773,HSE220,18.6297613359 chr2,89034794,89034994,HSE641,10.882267071 chr2,91750589,91750877,HSE327,20.2352669039 chr2,91762380,91762580,HSE468,12.6786942321 chr2,97019268,97019468,HSE786,8.3601047373 chr2,98758986,98759186,HSE679,16.1171102076 chr2,100697871,100698071,HSE706,13.479967802 chr2,100699949,100700149,HSE163,38.236433871 chr2,104094111,104094311,HSE59,33.1831421211 chr2,106095656,106095858,HSE390,15.6220351252 chr2,106554244,106554444,HSE497,18.2333367245 chr2,109968022,109968222,HSE763,10.4860988331 chr2,110515113,110515315,HSE19,48.1747064465 chr2,110806206,110806406,HSE977,8.2999349047 chr2,111400651,111400851,HSE808,9.0427754883 chr2,112020360,112020560,HSE444,14.0773788267 chr2,112221956,112222156,HSE720,15.6782160603 chr2,114395544,114395744,HSE67,33.2899481068 chr2,119068189,119068389,HSE104,27.8409422337 chr2,119071047,119071247,HSE25,58.9652879287 chr2,121088717,121088917,HSE726,14.2115751447 chr2,122336997,122337197,HSE249,20.8047512788 chr2,124279519,124279719,HSE495,11.9685617057 chr2,124730178,124730378,HSE30,48.1797064921 chr2,131094775,131094975,HSE988,12.5951506631 chr2,131484996,131485196,HSE76,33.2819734412 chr2,133300942,133301142,HSE997,7.8669044953 chr2,134090771,134090971,HSE180,24.5244184665 chr2,137008268,137008468,HSE307,16.6788740279 chr2,137015528,137015728,HSE947,7.5774875118 chr2,142934541,142934741,HSE345,21.8622427277 chr2,150036853,150037053,HSE807,12.9999546517 chr2,165778793,165778993,HSE358,17.0239953486 chr2,177391858,177392058,HSE260,19.1403997668 chr2,182327537,182327737,HSE681,17.5160573028 chr2,182393251,182393451,HSE40,38.4626716079 chr2,194117851,194118051,HSE321,18.0633419546 chr2,202563650,202563850,HSE626,12.3289416007 chr2,202741843,202742043,HSE359,20.9626551138 chr2,207294294,207294494,HSE670,11.1162813392 chr2,210363875,210364075,HSE734,12.3968615154 chr2,216306316,216306516,HSE868,14.1928417965 chr2,217522315,217522515,HSE293,35.0627763697 chr2,218641534,218641734,HSE82,37.7257060365 chr2,225243534,225243734,HSE416,20.099420911 chr2,229706577,229706777,HSE943,10.1343101996 chr2,231797519,231797723,HSE719,12.2696761634 chr2,232478862,232479063,HSE856,8.5861916221 chr2,236011384,236011584,HSE496,11.7277379839 chr2,238512362,238512563,HSE608,13.5849351928 chr2,240078429,240078629,HSE174,24.7169603423 chr2,241308943,241309143,HSE590,12.5097816417 chr2,241406758,241406958,HSE727,15.8620577497 chr20,1791874,1792074,HSE487,14.0309392186 chr20,1864025,1864225,HSE950,9.0977150918 chr20,1871245,1871445,HSE16,56.7490572952 chr20,2012791,2012991,HSE87,31.037092624 chr20,3629754,3629958,HSE215,20.9130441286 chr20,3660554,3660754,HSE193,24.974343769 chr20,4063903,4064104,HSE111,56.1553976804 chr20,14705232,14705432,HSE33,47.5410662718 chr20,16719908,16720108,HSE270,16.3543605271 chr20,32774062,32774262,HSE309,18.1246253801 chr20,34463418,34463619,HSE367,15.2757490918 chr20,34681486,34681692,HSE753,9.3728031841 chr20,34961484,34961684,HSE355,27.5542266393 chr20,36875508,36875708,HSE12,88.6429013544 chr20,39492043,39492243,HSE616,13.836833653 chr20,39504403,39504603,HSE791,11.0391746143 chr20,39522366,39522566,HSE522,15.4476576681 chr20,41278899,41279099,HSE130,24.1052112576 chr20,41281047,41281248,HSE138,25.1054094538 chr20,43678567,43678767,HSE118,27.5802502403 chr20,47354941,47355141,HSE829,10.0084428 chr20,48196544,48196744,HSE13,62.2017999936 chr20,48391843,48392070,HSE343,17.2886280147 chr20,48451672,48451872,HSE658,15.2435487162 chr20,57999712,57999912,HSE593,11.851459255 chr20,58010717,58010917,HSE888,8.4178547638 chr20,58028998,58029198,HSE589,11.8594187455 chr20,62892549,62892749,HSE22,49.3467939523 chr21,18183390,18183590,HSE846,12.0921264014 chr21,25470304,25470504,HSE848,9.043990116 chr21,29314093,29314293,HSE540,11.1333587451 chr21,29522195,29522395,HSE90,42.5669364456 chr21,30279211,30279411,HSE802,8.9817364292 chr21,31599287,31599487,HSE255,25.8534564105 chr21,34305184,34305384,HSE75,34.8701267573 chr21,40357115,40357316,HSE931,14.2521728213 chr21,40369917,40370117,HSE596,13.6512982833 chr21,42458559,42458759,HSE941,7.986093663 chr21,42461230,42461430,HSE46,32.6233218231 chr21,45610526,45610726,HSE373,19.1339980285 chr22,18647319,18647519,HSE600,15.4170005372 chr22,21387111,21387311,HSE465,16.7936897957 chr22,25800344,25800546,HSE855,15.133649847 chr22,27530972,27531172,HSE91,32.8296818218 chr22,27613121,27613321,HSE878,9.9026827262 chr22,30938555,30938755,HSE991,12.3953908195 chr22,37608742,37608942,HSE199,25.5079108823 chr22,38213774,38213975,HSE336,29.8698643259 chr22,38875187,38875387,HSE31,75.0968010157 chr22,45034999,45035200,HSE944,11.0058314418 chr22,47560535,47560735,HSE820,9.6159416626 chr22,49263785,49263985,HSE123,27.4756435262 chr3,1033708,1033908,HSE547,11.8167271684 chr3,4648618,4648819,HSE594,13.1354478948 chr3,6170925,6171125,HSE274,19.6422205175 chr3,9861617,9861817,HSE561,16.1351899583 chr3,20581162,20581362,HSE88,84.6550501758 chr3,22766006,22766206,HSE926,7.4746803691 chr3,25039886,25040088,HSE745,12.5677664568 chr3,32452491,32452691,HSE958,8.8781271039 chr3,34337067,34337267,HSE238,23.4517631822 chr3,44106796,44106996,HSE645,14.6078435944 chr3,45454944,45455144,HSE213,19.0172183728 chr3,50468482,50468682,HSE70,32.3982070336 chr3,50553584,50553784,HSE409,31.7674822371 chr3,50759518,50759718,HSE556,12.7286106999 chr3,51312927,51313129,HSE179,22.7291813917 chr3,54024155,54024359,HSE606,12.4118581011 chr3,55583333,55583544,HSE895,20.4730234134 chr3,57432272,57432472,HSE170,23.9015532829 chr3,57731978,57732178,HSE979,13.0317416952 chr3,58143711,58143912,HSE488,21.4279391783 chr3,59117232,59117432,HSE310,21.6692936097 chr3,59537533,59537733,HSE831,10.4335147498 chr3,60313490,60313690,HSE797,11.4508642018 chr3,60739661,60739861,HSE222,37.1649368806 chr3,61899965,61900165,HSE972,9.9582764633 chr3,62859461,62859661,HSE671,12.0850192866 chr3,62874394,62874594,HSE454,12.2791253714 chr3,63748080,63748280,HSE387,14.5767368616 chr3,74103231,74103431,HSE633,15.5075880796 chr3,85652655,85652855,HSE203,18.8184809601 chr3,97829189,97829389,HSE776,9.1102578423 chr3,103166013,103166213,HSE896,9.9523912683 chr3,105602785,105602985,HSE433,40.5785957197 chr3,107148262,107148462,HSE261,18.4043131499 chr3,109288204,109288404,HSE356,14.9310679943 chr3,111197315,111197518,HSE370,15.859503117 chr3,112219616,112219816,HSE368,15.0704271372 chr3,123405019,123405220,HSE115,27.2314354098 chr3,124880427,124880628,HSE579,14.8608441809 chr3,127740739,127740949,HSE314,26.0837216172 chr3,128256027,128256227,HSE630,13.9854481485 chr3,132829089,132829289,HSE474,15.9443427668 chr3,133123857,133124057,HSE471,13.3464634396 chr3,136537878,136538084,HSE505,14.6728277902 chr3,136915437,136915640,HSE584,13.4830219375 chr3,136922639,136922839,HSE304,16.1133399857 chr3,143048781,143048981,HSE663,12.6197529914 chr3,145155998,145156198,HSE963,7.937403129 chr3,150102651,150102851,HSE441,27.9319328699 chr3,152314041,152314241,HSE863,9.389594265 chr3,152446287,152446487,HSE202,21.6487393195 chr3,152497432,152497632,HSE782,15.2232552726 chr3,153501047,153501247,HSE973,8.9058148252 chr3,156106412,156106612,HSE298,17.4725292847 chr3,156467111,156467311,HSE984,12.8714197176 chr3,159583162,159583362,HSE245,24.3670741479 chr3,167647788,167647988,HSE162,23.142026511 chr3,167754605,167754805,HSE157,25.5019260862 chr3,168309609,168309809,HSE985,8.3878224111 chr3,170459966,170460166,HSE299,23.2572944826 chr3,171083365,171083565,HSE329,19.6170760846 chr3,171622769,171622969,HSE141,35.5269780554 chr3,173303292,173303492,HSE635,12.8762248581 chr3,173340053,173340253,HSE910,13.8353115715 chr3,175722586,175722786,HSE154,26.1510590855 chr3,177471354,177471554,HSE332,24.5244184665 chr3,184084866,184085066,HSE428,17.9640764471 chr3,192869895,192870095,HSE246,22.5875292012 chr3,194592300,194592501,HSE362,36.8838467218 chr3,196346498,196346699,HSE280,19.4679975855 chr3,197199171,197199371,HSE876,13.6753444396 chr4,1209278,1209519,HSE564,13.9004104602 chr4,1682431,1682632,HSE233,22.8144061287 chr4,2673293,2673493,HSE435,15.7039657822 chr4,5205616,5205843,HSE875,9.1366703212 chr4,5774976,5775176,HSE248,21.1824928836 chr4,7153027,7153227,HSE983,9.7283332501 chr4,7158073,7158273,HSE164,31.8768175016 chr4,7987860,7988060,HSE685,14.8308227861 chr4,8317712,8317912,HSE912,11.2750910314 chr4,10298688,10298888,HSE7,56.3001544011 chr4,10307533,10307733,HSE478,12.8133414228 chr4,10410626,10410826,HSE197,21.3709305662 chr4,16325714,16325914,HSE351,19.7714522278 chr4,16843354,16843554,HSE872,11.1479071197 chr4,17189915,17190115,HSE794,12.4374877889 chr4,17399671,17399871,HSE698,12.9259482624 chr4,18421836,18422036,HSE418,18.6650288814 chr4,20654001,20654201,HSE842,9.8868805638 chr4,35935735,35935935,HSE864,11.6965490821 chr4,37141687,37141887,HSE384,14.5996824189 chr4,37189108,37189308,HSE188,35.9761823699 chr4,38192503,38192704,HSE513,20.6670228424 chr4,41059770,41059970,HSE550,14.2940364222 chr4,41146900,41147100,HSE920,10.9173925032 chr4,42598985,42599185,HSE834,12.2213134666 chr4,43551753,43551953,HSE613,10.9664468122 chr4,52918093,52918293,HSE813,10.8199181309 chr4,67296659,67296859,HSE330,17.1184863176 chr4,68783414,68783614,HSE194,27.8865779207 chr4,73531199,73531399,HSE485,16.0641880211 chr4,79442149,79442349,HSE254,31.3053467034 chr4,96027806,96028006,HSE60,38.0420908281 chr4,98417455,98417655,HSE773,12.7094671878 chr4,102826820,102827020,HSE954,9.8953082642 chr4,108956348,108956548,HSE196,28.1854857987 chr4,109035842,109036042,HSE574,15.4815826792 chr4,109331833,109332033,HSE880,8.5800439974 chr4,112855557,112855758,HSE873,8.9177752517 chr4,113045048,113045248,HSE718,12.9338359083 chr4,115673675,115673875,HSE908,9.0213984225 chr4,126033815,126034015,HSE266,27.8144026005 chr4,126926133,126926333,HSE624,13.8939469933 chr4,134018135,134018335,HSE591,13.1426859966 chr4,135498284,135498484,HSE289,16.9047451361 chr4,139144837,139145037,HSE858,11.0683425726 chr4,142822079,142822279,HSE291,16.8719939446 chr4,143883214,143883420,HSE125,23.7564028153 chr4,155022297,155022497,HSE486,24.4008504204 chr4,155876015,155876219,HSE567,14.3792053309 chr4,156523530,156523730,HSE335,17.9101352191 chr4,163926681,163926881,HSE583,13.7123935 chr4,165404029,165404229,HSE965,10.3169505717 chr4,171168842,171169042,HSE500,12.3708601884 chr4,176356648,176356848,HSE586,12.5175850781 chr4,181756449,181756649,HSE116,25.0123586931 chr4,185517517,185517717,HSE768,10.7243756024 chr4,186037200,186037400,HSE849,9.15728802 chr4,187112549,187112749,HSE73,29.6147089784 chr4,188809131,188809331,HSE127,26.3736706202 chr5,9015815,9016015,HSE754,10.9860464939 chr5,9140785,9140986,HSE34,39.8342153912 chr5,9284766,9284966,HSE159,44.8345695381 chr5,9423793,9423993,HSE397,23.9014518508 chr5,9544694,9544894,HSE976,18.4787378901 chr5,13572514,13572715,HSE970,7.981996643 chr5,14844977,14845178,HSE623,11.8533435499 chr5,26843843,26844043,HSE609,10.9929164023 chr5,35374800,35375000,HSE992,11.2155834064 chr5,36059728,36059928,HSE743,9.7015183684 chr5,38011944,38012144,HSE182,23.9400859933 chr5,39421419,39421619,HSE825,14.5866459668 chr5,39533959,39534159,HSE760,9.9360929783 chr5,43066506,43066706,HSE288,64.294192312 chr5,50917138,50917340,HSE313,22.7435698695 chr5,51008092,51008292,HSE766,10.2876577874 chr5,58130163,58130363,HSE686,14.7084178919 chr5,68296916,68297116,HSE57,43.9761647554 chr5,70529394,70529594,HSE292,16.3167607327 chr5,73557824,73558024,HSE283,26.1537486394 chr5,81951100,81951300,HSE838,11.1124928906 chr5,82305319,82305520,HSE256,21.9987593589 chr5,93459233,93459433,HSE79,88.077239222 chr5,96180256,96180457,HSE112,28.97133338 chr5,98859253,98859453,HSE457,16.1598314579 chr5,102729064,102729264,HSE89,35.4121997783 chr5,107225179,107225379,HSE752,12.8723311418 chr5,108006706,108006906,HSE167,20.5736395644 chr5,112562162,112562362,HSE146,28.4736858728 chr5,115731439,115731639,HSE662,14.441740722 chr5,115870785,115870985,HSE889,11.1682472441 chr5,121403180,121403380,HSE738,10.4404525204 chr5,123709637,123709837,HSE385,34.5371219842 chr5,124534387,124534587,HSE189,41.4113583783 chr5,126015956,126016156,HSE350,48.9260098909 chr5,130651056,130651256,HSE353,19.9406119279 chr5,131054387,131054588,HSE275,16.8672736168 chr5,138872592,138872792,HSE97,33.7294648775 chr5,139113346,139113546,HSE499,24.5461507056 chr5,139503491,139503691,HSE135,22.5832471238 chr5,140365894,140366094,HSE477,22.7804027056 chr5,152496425,152496625,HSE297,23.9517433229 chr5,153157431,153157632,HSE994,7.2712465887 chr5,154985156,154985356,HSE27,47.3698229496 chr5,156798061,156798261,HSE421,18.0953061141 chr5,159614141,159614341,HSE504,16.644367563 chr5,169678151,169678351,HSE205,23.5872306039 chr5,169969996,169970196,HSE252,26.7061548422 chr5,170556363,170556585,HSE81,53.3489093817 chr5,170880119,170880319,HSE533,21.0213665516 chr5,176794222,176794423,HSE599,23.2425155735 chr5,177712082,177712283,HSE921,9.6878527385 chr6,1720956,1721156,HSE961,13.9546110024 chr6,2908962,2909162,HSE900,9.4385437272 chr6,3624882,3625082,HSE49,38.6547390654 chr6,6477697,6477897,HSE425,14.0308308131 chr6,6927996,6928198,HSE573,13.2644125542 chr6,7261536,7261736,HSE899,20.57594766 chr6,8857485,8857685,HSE226,19.7424552164 chr6,11764188,11764388,HSE423,13.6453117051 chr6,12657164,12657364,HSE541,24.5587040398 chr6,13069178,13069378,HSE148,25.4358868167 chr6,13071666,13071866,HSE328,21.7306804565 chr6,13440803,13441003,HSE854,11.7878221637 chr6,13605303,13605503,HSE439,16.0336294076 chr6,15357986,15358186,HSE772,12.1002596677 chr6,15953729,15953929,HSE223,22.2692616975 chr6,20879847,20880047,HSE789,11.3511396601 chr6,22074016,22074216,HSE830,17.8828299952 chr6,22223835,22224035,HSE847,11.3189534245 chr6,22754318,22754518,HSE765,10.933963726 chr6,24191674,24191874,HSE17,64.4494432065 chr6,24355724,24355924,HSE822,12.2330307591 chr6,24684045,24684245,HSE68,40.6121039818 chr6,24908692,24908892,HSE52,36.3357402285 chr6,24981608,24981808,HSE460,14.7455299562 chr6,26866614,26866814,HSE316,17.4817827982 chr6,26987817,26988017,HSE301,17.0996119934 chr6,28953271,28953471,HSE532,18.2243327433 chr6,30163047,30163247,HSE668,9.9208283821 chr6,32862428,32862628,HSE883,10.9304672886 chr6,33048550,33048750,HSE100,28.3569655356 chr6,37190063,37190263,HSE225,25.1598854782 chr6,37752532,37752732,HSE190,21.4887428904 chr6,37839872,37840072,HSE692,15.9827010242 chr6,42104428,42104628,HSE107,67.5217188687 chr6,42125896,42126096,HSE836,11.8802206566 chr6,48152098,48152298,HSE317,16.53498743 chr6,48440896,48441096,HSE410,20.2304692131 chr6,48531624,48531824,HSE14,56.1553976804 chr6,48595126,48595327,HSE8,86.3398854446 chr6,57207153,57207353,HSE779,15.3463418546 chr6,58265076,58265285,HSE632,13.3681109557 chr6,68599806,68600006,HSE526,12.8237395122 chr6,71584109,71584310,HSE114,27.0742020553 chr6,77915769,77915969,HSE236,16.9378739354 chr6,87169783,87169984,HSE546,11.573392587 chr6,89894075,89894277,HSE647,13.1942619616 chr6,90904900,90905100,HSE491,25.8304194594 chr6,92350596,92350796,HSE653,17.364572389 chr6,101840815,101841015,HSE131,28.2436702832 chr6,105240470,105240670,HSE604,18.8779663264 chr6,107221861,107222061,HSE844,13.1640641167 chr6,107611759,107611959,HSE904,13.5023269104 chr6,112619369,112619569,HSE369,17.0366032561 chr6,114999140,114999341,HSE821,10.3959314865 chr6,116138273,116138476,HSE730,13.0350910622 chr6,116621676,116621877,HSE929,9.2537035432 chr6,131428060,131428260,HSE284,18.8889226922 chr6,132596060,132596260,HSE990,8.2742348935 chr6,138104942,138105142,HSE253,23.0468563465 chr6,140757614,140757815,HSE967,10.1788738588 chr6,143968529,143968729,HSE852,8.5873299746 chr6,147322278,147322478,HSE268,19.7936739006 chr6,151695336,151695536,HSE519,19.0160524829 chr6,157265666,157265866,HSE119,24.7735526841 chr6,161392326,161392526,HSE334,18.7149756358 chr6,161740337,161740537,HSE713,9.6675137739 chr6,162838242,162838442,HSE132,26.1781329147 chr6,166188920,166189120,HSE417,14.5239466546 chr6,167892584,167892784,HSE814,8.7865263368 chr6,169077927,169078127,HSE110,27.1403888015 chr6,170794092,170794292,HSE524,13.6109134035 chr7,4198833,4199033,HSE801,11.1292518601 chr7,4949917,4950117,HSE172,21.4927855963 chr7,13461438,13461638,HSE228,17.5121934963 chr7,22122623,22122823,HSE930,12.3048991783 chr7,23062178,23062378,HSE509,25.1202244572 chr7,23246200,23246400,HSE257,22.9981606665 chr7,25559731,25559931,HSE966,8.5262161829 chr7,36664999,36665199,HSE605,16.0994530522 chr7,37372863,37373063,HSE204,19.2684485089 chr7,38492364,38492564,HSE597,12.8932215725 chr7,40616066,40616266,HSE380,17.182635963 chr7,47064486,47064686,HSE577,13.0570408987 chr7,47084856,47085056,HSE576,16.6838216652 chr7,48031471,48031671,HSE740,11.7452011534 chr7,48748205,48748405,HSE98,30.5044348782 chr7,66050120,66050320,HSE276,21.6755649273 chr7,66213153,66213353,HSE534,13.7182502443 chr7,66223024,66223224,HSE364,16.185690078 chr7,74867660,74867860,HSE618,16.9538639948 chr7,77015807,77016008,HSE415,18.6613812869 chr7,78390011,78390211,HSE602,16.2431492916 chr7,100434184,100434384,HSE869,13.0394840372 chr7,101616503,101616703,HSE951,9.2324134965 chr7,102153402,102153602,HSE975,9.0245761817 chr7,110397521,110397721,HSE916,12.3054838817 chr7,110400563,110400763,HSE338,16.3230063175 chr7,116607477,116607678,HSE621,18.3522646727 chr7,118072236,118072438,HSE694,11.1158534833 chr7,125587404,125587604,HSE455,17.556648341 chr7,145100201,145100414,HSE401,15.6280338065 chr7,145839272,145839472,HSE171,20.1680033829 chr7,145844066,145844266,HSE9,54.1909479525 chr7,147449071,147449271,HSE704,10.2463146144 chr7,148178409,148178609,HSE835,10.6172870294 chr7,151235436,151235636,HSE365,18.0341069228 chr8,2377506,2377706,HSE181,20.7659351902 chr8,2511126,2511326,HSE320,15.27069181 chr8,7266669,7266869,HSE819,8.435349452 chr8,13356648,13356848,HSE281,18.4464980795 chr8,14336890,14337104,HSE66,46.656249178 chr8,23032626,23032826,HSE412,26.2529950816 chr8,26601555,26601755,HSE890,11.220125934 chr8,26628311,26628511,HSE788,10.9551178433 chr8,28009163,28009363,HSE510,15.0235522925 chr8,30385906,30386106,HSE629,17.1134130395 chr8,34312488,34312688,HSE178,30.2724339491 chr8,49930877,49931077,HSE507,18.1489727778 chr8,50149648,50149848,HSE518,13.7760589052 chr8,52875070,52875270,HSE501,15.5123958666 chr8,53660034,53660234,HSE93,37.3464806395 chr8,54605619,54605820,HSE684,14.2519866729 chr8,62892804,62893004,HSE121,31.87775364 chr8,73254670,73254870,HSE185,23.4900882124 chr8,73412628,73412828,HSE38,37.3035911391 chr8,75862050,75862250,HSE620,13.858825618 chr8,88877867,88878069,HSE563,12.7713866408 chr8,92115315,92115515,HSE530,14.8449505644 chr8,92248603,92248804,HSE691,15.9124555229 chr8,93852146,93852346,HSE290,32.7317983798 chr8,95213282,95213482,HSE494,13.4997136969 chr8,98387996,98388197,HSE628,12.1439641326 chr8,105236101,105236301,HSE969,9.7479394792 chr8,115779521,115779721,HSE688,12.8255728825 chr8,122167781,122167981,HSE371,17.391031161 chr8,125610826,125611026,HSE165,35.3095256409 chr8,131652848,131653048,HSE363,19.456186422 chr8,134045654,134045854,HSE168,31.8082809938 chr8,134829150,134829350,HSE646,10.9003096576 chr8,144094801,144095001,HSE717,15.3147108573 chr8,144343458,144343659,HSE72,32.0323018157 chr8,145987786,145988059,HSE451,17.6753394576 chr9,7510288,7510488,HSE312,33.7426439062 chr9,10593086,10593286,HSE414,16.6788740279 chr9,11458448,11458648,HSE843,9.0784819552 chr9,12117105,12117305,HSE142,27.950209127 chr9,14993242,14993442,HSE634,14.4731722458 chr9,16301282,16301482,HSE102,50.9103305769 chr9,17765448,17765655,HSE739,18.2392565514 chr9,20825252,20825452,HSE946,12.4789871155 chr9,33402443,33402643,HSE555,16.1608716083 chr9,33523607,33523818,HSE224,19.2005783714 chr9,34170779,34170979,HSE341,24.6371580044 chr9,41824106,41824306,HSE769,10.5181109381 chr9,42019392,42019592,HSE28,41.4263636489 chr9,44245346,44245546,HSE32,37.5468627247 chr9,46661510,46661710,HSE378,18.5997971629 chr9,67872401,67872601,HSE272,19.7212214429 chr9,69651218,69651418,HSE15,56.5573849515 chr9,69654841,69655041,HSE50,50.1850231356 chr9,69723884,69724084,HSE126,32.6894006406 chr9,70077265,70077469,HSE357,19.5503409497 chr9,70090005,70090205,HSE56,47.863549167 chr9,72158059,72158265,HSE736,10.8002448051 chr9,72749929,72750129,HSE667,9.6151470352 chr9,81641307,81641507,HSE565,12.5343408216 chr9,85896509,85896709,HSE360,15.976780491 chr9,87992926,87993126,HSE682,10.0728691654 chr9,91288879,91289079,HSE570,20.1264215633 chr9,93232164,93232364,HSE267,16.2689597218 chr9,97610366,97610566,HSE571,14.362464282 chr9,97933380,97933580,HSE660,11.3259473431 chr9,100000625,100000826,HSE473,16.0316421967 chr9,100937326,100937526,HSE502,14.7363484454 chr9,112871691,112871891,HSE427,20.2200008236 chr9,116605468,116605668,HSE24,44.0402200911 chr9,118163144,118163344,HSE10,116.5676393698 chr9,136738864,136739065,HSE828,21.7259174217 chr9,136826692,136826892,HSE823,8.8396928196 chr9,138884709,138884909,HSE749,21.506795832 chr9,140320110,140320310,HSE558,17.6159627193 chrX,191631,191831,HSE375,22.299626784 chrX,197891,198101,HSE318,51.9353250981 chrX,199983,200184,HSE711,18.1602756116 chrX,9247138,9247338,HSE915,8.9889411466 chrX,9294553,9294753,HSE636,15.8583274764 chrX,13467472,13467672,HSE625,13.3123255447 chrX,22410208,22410409,HSE982,9.6853480348 chrX,29231637,29231837,HSE610,12.7761366615 chrX,30489623,30489824,HSE137,24.5548014378 chrX,31825117,31825317,HSE99,25.9398284883 chrX,36439734,36439934,HSE323,16.5354248431 chrX,36467884,36468084,HSE402,16.6156581564 chrX,40432956,40433156,HSE933,11.0828028555 chrX,40810144,40810344,HSE106,48.7539914758 chrX,49953599,49953799,HSE295,25.7542688302 chrX,57163623,57163823,HSE503,22.7827962324 chrX,114733851,114734051,HSE986,7.9927176453 chrX,124903682,124903882,HSE436,12.6648421132 chrX,128211486,128211686,HSE815,14.3611432709 chrX,128270781,128270981,HSE952,10.1358588237 chrX,130964620,130964822,HSE980,11.9103854587 ================================================ FILE: intro-to-awk/data/example.fastq ================================================ @ERR117184_2.14044282 CTGGTGCTCAGTCATTTTGCTAGATTGTAGCTCACCATTGCCTCTCTGCCTGCATTGTGCACTCCAATCCCTCAGCAGCTACAAAAACACTTTGTCAACTCCAGATCGG + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117185_2.23793537 TTGTTCAATTTTTTTAGTAGTTTTAGAGTAATTAATGAGCTTTGAGGTCACTTAACAATAAAAGCATATTTTAAAGTAAAGGTTGCTGATCAACAGTCTAATTTTCATA + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117166_2.4649205 ACTTAACAATAAAAGCATATTTTAAAGTAAAGGTT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117185_2.949669 ATGCTTTTATTGTTAAGTGACCTCAAAGCTCATTAATTACTCTAAAACTACTAAAAAAATTGAACAAAAAAGTATTTCCAGTAATCAAGATTTTCTTCATGCCCTAGAT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117163_2.2292219 AAATCGTAAGTGAAGCAACAGAATTCAGAACTAAA + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117165_2.36526615 AACGCTAAGGTTATATTAAAATGTTTCTTATGTTG + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117174_2.23007391 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117175_2.11345437 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117176_2.30285510 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117177_2.33680511 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII ================================================ FILE: intro-to-awk/data/hg38.genome ================================================ chrom size 1 248956422 2 242193529 3 198295559 4 190214555 5 181538259 6 170805979 7 159345973 X 156040895 8 145138636 9 138394717 11 135086622 10 133797422 12 133275309 13 114364328 14 107043718 15 101991189 16 90338345 17 83257441 18 80373285 20 64444167 19 58617616 Y 57227415 22 50818468 21 46709983 15_KI270905v1_alt 5161414 6_GL000256v2_alt 4929269 6_GL000254v2_alt 4827813 6_GL000251v2_alt 4795265 6_GL000253v2_alt 4677643 6_GL000250v2_alt 4672374 6_GL000255v2_alt 4606388 6_GL000252v2_alt 4604811 17_KI270857v1_alt 2877074 16_KI270853v1_alt 2659700 16_KI270728v1_random 1872759 17_GL000258v2_alt 1821992 5_GL339449v2_alt 1612928 14_KI270847v1_alt 1511111 17_KI270908v1_alt 1423190 14_KI270846v1_alt 1351393 5_KI270897v1_alt 1144418 7_KI270803v1_alt 1111570 19_GL949749v2_alt 1091841 19_KI270938v1_alt 1066800 19_GL949750v2_alt 1066390 19_GL949748v2_alt 1064304 19_GL949751v2_alt 1002683 19_GL949746v1_alt 987716 19_GL949752v1_alt 987100 8_KI270821v1_alt 985506 1_KI270763v1_alt 911658 6_KI270801v1_alt 870480 19_GL949753v2_alt 796479 19_GL949747v2_alt 729520 8_KI270822v1_alt 624492 4_GL000257v2_alt 586476 12_KI270904v1_alt 572349 4_KI270925v1_alt 555799 15_KI270852v1_alt 478999 15_KI270727v1_random 448248 9_KI270823v1_alt 439082 15_KI270850v1_alt 430880 1_KI270759v1_alt 425601 12_GL877876v1_alt 408271 Un_KI270442v1 392061 17_KI270862v1_alt 391357 15_GL383555v2_alt 388773 19_GL383573v1_alt 385657 4_KI270896v1_alt 378547 4_GL383528v1_alt 376187 17_GL383563v3_alt 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36723 8_KI270820v1_alt 36640 1_KI270707v1_random 32032 Un_KI270579v1 31033 Un_KI270752v1 27745 Un_KI270512v1 22689 Un_KI270322v1 21476 M 16569 Un_GL000226v1 15008 Un_KI270311v1 12399 Un_KI270366v1 8320 Un_KI270511v1 8127 Un_KI270448v1 7992 Un_KI270521v1 7642 Un_KI270581v1 7046 Un_KI270582v1 6504 Un_KI270515v1 6361 Un_KI270588v1 6158 Un_KI270591v1 5796 Un_KI270522v1 5674 Un_KI270507v1 5353 Un_KI270590v1 4685 Un_KI270584v1 4513 Un_KI270320v1 4416 Un_KI270382v1 4215 Un_KI270468v1 4055 Un_KI270467v1 3920 Un_KI270362v1 3530 Un_KI270517v1 3253 Un_KI270593v1 3041 Un_KI270528v1 2983 Un_KI270587v1 2969 Un_KI270364v1 2855 Un_KI270371v1 2805 Un_KI270333v1 2699 Un_KI270374v1 2656 Un_KI270411v1 2646 Un_KI270414v1 2489 Un_KI270510v1 2415 Un_KI270390v1 2387 Un_KI270375v1 2378 Un_KI270420v1 2321 Un_KI270509v1 2318 Un_KI270315v1 2276 Un_KI270302v1 2274 Un_KI270518v1 2186 Un_KI270530v1 2168 Un_KI270304v1 2165 Un_KI270418v1 2145 Un_KI270424v1 2140 Un_KI270417v1 2043 Un_KI270508v1 1951 Un_KI270303v1 1942 Un_KI270381v1 1930 Un_KI270529v1 1899 Un_KI270425v1 1884 Un_KI270396v1 1880 Un_KI270363v1 1803 Un_KI270386v1 1788 Un_KI270465v1 1774 Un_KI270383v1 1750 Un_KI270384v1 1658 Un_KI270330v1 1652 Un_KI270372v1 1650 Un_KI270548v1 1599 Un_KI270580v1 1553 Un_KI270387v1 1537 Un_KI270391v1 1484 Un_KI270305v1 1472 Un_KI270373v1 1451 Un_KI270422v1 1445 Un_KI270316v1 1444 Un_KI270338v1 1428 Un_KI270340v1 1428 Un_KI270583v1 1400 Un_KI270334v1 1368 Un_KI270429v1 1361 Un_KI270393v1 1308 Un_KI270516v1 1300 Un_KI270389v1 1298 Un_KI270466v1 1233 Un_KI270388v1 1216 Un_KI270544v1 1202 Un_KI270310v1 1201 Un_KI270412v1 1179 Un_KI270395v1 1143 Un_KI270376v1 1136 Un_KI270337v1 1121 Un_KI270335v1 1048 Un_KI270378v1 1048 Un_KI270379v1 1045 Un_KI270329v1 1040 Un_KI270419v1 1029 Un_KI270336v1 1026 Un_KI270312v1 998 Un_KI270539v1 993 Un_KI270385v1 990 Un_KI270423v1 981 Un_KI270392v1 971 Un_KI270394v1 970 ================================================ FILE: intro-to-awk/index.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "## Manipulating files with AWK\n", "### Bioinformatics Coffee Hour - April 14, 2020\n", "\n", "### What is awk?\n", "Invented in the 1970's, [awk](https://en.wikipedia.org/wiki/AWK) is a scripting language included in most Unix-like operating systems. It specializes in one-liner programs and manipulating text files.\n", "\n", "In many cases, if you're parsing information from a text file (such as a [BED](https://en.wikipedia.org/wiki/BED_(file_format)) file, [FASTA](https://en.wikipedia.org/wiki/FASTA) file, etc.), you could write a Python script...or you could do it with awk in a single line!\n", "\n", "### Syntax\n", "awk scripts are organized as:\n", "\n", "`awk 'pattern { action; other action }' file`\n", "\n", "Meaning that every time that the pattern is true, awk will execute the action in the brackets.\n", "If no pattern is specified, the action will be taken for every line in the input file, e.g. the following command prints every line:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "The two most important patterns are `BEGIN` and `END`, which tell the action to take place before any lines are read and after the last line." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{sum=0} {sum+=1} END {print sum}' data/hg38.genome" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ " The above line sets a variable at the start of the script, adds 1 to it every line, then prints its value at the end." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "If a variable hasn't been initialized, it is treated as 0 in numeric expressions, and an empty string in string expressions—awk will not print an error!\n", "So the following awk script also prints the number of lines in the file data/hg38.genome:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{sum+=1} END {print sum}' data/hg38.genome" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Input and output\n", "Input to awk is split into **records** and **fields**.\n", "- By default, **records** are separated by newline character, i.e # of records = # of lines in input file\n", "- Each record is subdivided into **fields**, i.e. columns, as determined by the field separator (see below)\n", "\n", "There are several important built-in variable in awk.\n", "The fields (columns) of each record are referred to by `$number`, so the first column would be `$1`, second would be `$2`, etc. `$0` refers to the entire record.\n", "\n", "So to print the second column of each line in the file, we'd use:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print $2}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "And if we wanted to print the second then the first:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print $2,$1}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note that when the different fields are separated with commas in the `print` statement, they are joined by the output field separator (the **OFS** variable, described below), which is by default a space.\n", "If the comma is omitted between fields (e.g., `awk '{print $2 $1}'`, they are concatenated without a separator.\n", "\n", "We can also print strings using using quotation marks:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print \"First column:\" $1}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Which for every line of the file will print the text \"First column:\" followed by the value in the first field." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "---\n", "awk has several other built-in variables that are very useful for parsing text, including:\n", "\n", "| | |\n", "---|---|\n", "| **FS** | field separator (default: white space) |\n", "| **OFS** | output field separator, i.e. what character separates fields when printing|\n", "| **RS** | record separator, i.e. what character records are split on (default: new line) |\n", "| **ORS** | output record separator |\n", "| **NR** | number of records in input (# lines by default) |\n", "\n", "Assigning to a field causes the entire record ($0) to be recomputed using **OFS**. We can use this to convert between file formats, e.g. make a comma-separated text file into a tab-separated file. First, let's look at the first few lines on our comma-separated file using `head`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/enhancers.csv" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now let's convert the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\",\" ; OFS=\"\\t\"} {$1 = $1; print $0}' data/enhancers.csv | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Conditionals and pattern matching\n", "Like other programming languages, awk allows conditional matching with if/else statements.\n", "\n", "`awk '{if(condition) action; else other_action}'`\n", "\n", "awk uses the following conditional operators:\n", "\n", "| | |\n", "|-|-|\n", "|==|equal to|\n", "|!=|not equal to|\n", "|>|greater than|\n", "|>=|greater than or equal to|\n", "|<|less than|\n", "|<=|less than or equal to|\n", "|&&|AND|\n", "| \\|\\| |OR|\n", "| ! | NOT |\n", "\n", "In addition, awk also supports string matching using regular expressions, using the following expressions:\n", "\n", "| | |\n", "|-|-|\n", "|\\~|matches|\n", "|!~|does not match|\n", "\n", "For string matching, the pattern being matched must be enclosed by slashes, like so:\n", "\n", "`awk '{if($1 ~ /pattern/) print}'`\n", "\n", "Note that if an action isn't specified, the default action is `{print}`, so the previous awk command is equivalent to the following, which specifies only a pattern expression:\n", "\n", "`awk '$1 ~ /pattern/'`\n", "\n", "---\n", "### Example uses:\n", "\n", "- Count number of sequences in a FASTQ file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'END{print NR/4}' data/example.fastq" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: this is technically safer than using grep, as you don't have to worry about accidentally counting the quality line.\n", "\n", "- Only print annotations on a specific scaffold (chr1) that fall between 1Mb and 2Mb from a BED annotation file. First let's look at the first few lines of the file using `head`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now subset the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\";OFS=\"\\t\"} {if($1 == \"chr1\" && $2 >=1000000 && $2 <= 2000000) print}' data/Homo_sapiens_ucscGenes.subset.bed | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: when we specify that we only want annotations from chr1, we're using exact match (`== \"chr1\"`) and not pattern match (`~ /chr1/`)...why is this??" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Only print lines of GFF annotation file that match the string \"exon\" in their third column. Again, let's look at the first few lines of the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/Homo_sapiens.GRCh38.subset.gff3" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Then subset the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"} {if($3 ~ /exon/) print $0}' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Convert from GFF (genome feature file) to BED file " ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep -v '^#' data/Homo_sapiens.GRCh38.subset.gff3 | awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} {print $1,$4-1,$5}' | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: remember that BED and GFF files have different coordinate systems, i.e. BED start coordinate is 0 based, half-open, GFF is 1-based inclusive! Also, we are first using grep to skip the header lines in the GFF file.\n", "\n", "Alternatively, you could do the whole thing with only awk, no grep required:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} !/^#/ {print $1,$4-1,$5}' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "With this command, `!/^#/` is a pattern (like `BEGIN` or `END`) that tell awk to execute the print statement when the start of the line does not match a `#`. Use whichever makes the most sense to you!\n", "\n", "### Practice\n", "Using awk:\n", "\n", "* Pull out only the CDS annotations (i.e. has \"CDS\" in the 3rd column) from the GFF file data/Homo_sapiens.GRCh38.subset.gff3 and output them in BED format" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Try it*" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '...' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Solution*" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "jupyter": { "source_hidden": true } }, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} {if($3 ~ /CDS/) print $1,$4-1,$5}' data/Homo_sapiens.GRCh38.subset.gff3" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Calculate the average length of gene annotations from the file data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Try it*" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '...' data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Solution*" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "jupyter": { "source_hidden": true } }, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; sum=0} {len=$3-$2; sum=sum+len} END{print sum/NR}' data/Homo_sapiens_ucscGenes.subset.bed" ] } ], "metadata": { "kernelspec": { "display_name": "Bash", "language": "bash", "name": "bash" }, "language_info": { "codemirror_mode": "shell", "file_extension": ".sh", "mimetype": "text/x-sh", "name": "bash" } }, "nbformat": 4, "nbformat_minor": 4 } ================================================ FILE: intro-to-r/binder/Dockerfile ================================================ FROM rocker/binder:3.6.3 COPY --chown=rstudio:rstudio ./intro-to-r ${HOME} ================================================ FILE: intro-to-r/data/mbta_bus.tsv ================================================ route type cost.per.pax ridership pax.per.trip 1 Key 0.63 13306 416 4 Commuter 4.38 459 21 5 Community 2.99 156 5 7 Local 1.52 3893 177 8 Local 2.02 3844 85 9 Local 1.21 5980 187 10 Local 1.75 3184 398 11 Local 1.84 3312 237 14 Local 2.66 1285 22 15 Key 1.11 6227 111 16 Local 0.99 5100 2550 17 Local 0.71 3461 115 18 Local 2.71 630 16 19 Local 1.07 3591 211 21 Local 0.57 4696 90 22 Key 1.05 8151 170 23 Key 1 11687 899 24 Local 1.3 1725 21 26 Local 0.8 1960 41 27 Local 1.13 929 22 28 Key 0.8 13550 387 29 Local 1.71 2242 90 30 Local 1.17 2230 60 31 Local 1.07 4506 90 32 Key 1.02 10130 103 33 Local 1.67 1206 33 34 Local 2.43 5959 60 35 Local 1.75 2368 103 36 Local 1.47 3245 54 37 Local 2.1 1481 53 38 Local 2.29 1074 21 39 Key 0.72 15018 406 40 Local 1.54 1360 22 41 Local 1.84 2336 10 42 Local 0.8 2995 73 43 Local 1.93 1885 209 44 Local 1.28 3440 63 45 Local 1.53 3326 47 47 Local 1.41 4539 60 50 Local 1.74 1226 19 51 Local 1.95 1956 29 52 Local 4.97 747 22 55 Local 2.34 956 7 57 Key 0.86 12081 263 59 Local 2.71 1363 17 60 Local 2.99 1418 709 62 Local 2.53 1609 34 64 Local 2.33 1826 37 65 Local 1.43 2250 30 66 Key 0.79 13630 147 67 Local 2.98 666 4 68 Local 2.23 491 8 69 Local 0.82 3131 10 70 Local 1.96 7021 195 71 Key 1.35 5488 157 72 Local 3.05 808 31 73 Key 1.75 5834 46 74 Local 2.34 1144 29 75 Local 1.88 556 4 76 Local 3.67 1156 17 77 Key 1.95 7253 165 78 Local 3.6 1550 20 79 Local 3.05 1054 12 80 Local 2.35 1932 23 83 Local 1.49 2257 9 84 Commuter 2 356 9 85 Local 1.82 599 33 86 Local 1.07 5844 76 87 Local 1.17 3612 82 88 Local 0.89 3883 75 89 Local 1.01 3801 29 90 Local 1.75 1225 7 91 Local 1.01 1840 22 92 Local 2.68 1251 13 93 Local 1.03 4632 39 94 Local 2.02 1429 33 95 Local 2.2 1737 25 96 Local 2.09 1850 19 97 Local 2.12 841 4 99 Local 2.45 1452 12 100 Local 2.31 953 5 101 Local 1.04 4878 49 104 Local 0.9 4008 43 105 Local 1.91 983 11 106 Local 1.52 2682 47 108 Local 1.17 3166 15 109 Local 1.04 3697 127 110 Local 1.51 2885 40 111 Key 1.06 11695 38 112 Local 2.98 1367 13 114 Local 0.83 483 1 116 Key 0.88 5077 26 117 Key 0.73 4584 38 119 Local 2.16 1101 10 120 Local 1.18 3071 20 121 Commuter 1.1 443 1 131 Local 3.12 633 5 132 Local 2.49 796 3 134 Local 2.21 2186 18 136 Local 3.18 1076 16 137 Local 2.91 1028 11 170 Commuter 5.56 87 22 171 Commuter 3.23 38 0 210 Local 3.06 807 6 211 Local 2.72 890 3 212 Local 2.62 268 3 214 Local 1.35 1371 6 215 Local 2.3 1668 8 216 Local 2.65 1204 13 217 Local 7.19 223 8 220 Local 3.92 1512 6 221 Local 3.55 105 1 222 Local 2.99 1605 7 225 Local 1.86 2859 15 230 Local 3.3 1532 12 236 Local 3.56 597 2 238 Local 2.15 1894 7 240 Local 2.68 2840 13 245 Local 4.2 551 28 325 Express 7.17 289 8 326 Express 3.87 471 8 350 Local 3.36 1849 21 351 Commuter 6.33 246 11 352 Express 3.14 382 11 354 Express 5.16 764 40 411 Local 1.91 1181 39 424 Commuter 2.35 225 3 426 Local 2.14 2006 334 428 Commuter 3.5 171 7 429 Local 3.11 1559 16 430 Local 2.37 1228 26 431 Local 1.74 141 2 434 Express 2.17 75 1 435 Local 4.22 767 11 436 Local 4.44 790 790 439 Commuter 9.76 88 5 441 Local 1.86 1442 8 442 Local 1.8 2112 45 448 Commuter 4.47 162 9 449 Commuter 4.71 181 7 450 Local 2.6 1549 20 451 Local 6.34 323 25 455 Local 1.47 1797 20 456 Local 3.38 259 3 459 Local 2.75 1085 16 465 Local 4.9 348 87 468 Local 5.89 30 3 501 Express 2.68 1639 18 502 Express 1.34 1180 16 503 Express 3.45 347 9 504 Express 3.23 1452 19 505 Express 3.95 1042 15 553 Local 3.23 789 34 554 Local 4.95 618 14 556 Commuter 3.66 490 4 558 Commuter 4.11 407 7 714 Local 2.55 228 4 716 Local 4.19 106 1 201.202 Local 2.26 1582 22 712.713 Local 2.72 1597 27 CT1 Local 0.94 2261 33 CT2 Local 1.8 2580 31 CT3 Local 5.11 708 17 ================================================ FILE: intro-to-r/index.Rmd ================================================ --- title: "Intro to R" subtitle: "Bioinformatics Coffee Hour" date: "Augus 4, 2020" author: "Danielle Khost" output: html_document --- ##Setting up our working directory and saving objects vs files R is a functional programming language, which means that most of what one does is apply functions to objects. We will begin with a very brief introduction to R objects and how functions work, and then focus on getting data into R, manipulating that data in R, plotting, and analysis. First, we need to make sure we are working out of the correct directory. You can see the working directory above the console in RStudio, or use the `getwd()` function to see the current working directory. If you are not currently working in a good location to read and save files, you can use `setwd()` to change the working directory to the location of your choice. ```{r} getwd() ``` ## Vectors Let's start by creating one of the simplest R objects, a vector of numbers. ```{r} ``` v1 is an *object* which we created by using the ```<-``` operator (less followed by a dash). v1 now contains the output of the *function* ```c(1,2,3,4,5)``` (c, for **c**ombined, combines elements into a vector Let's display the contents of v1: ```{r} ``` We can also just type the name of an object (in this case ```v1```) to display it. Let's make a few more objects: ```{r} ``` With this last variable, we had to use ```""``` to specify that we want to create a character vector. Otherwise it thinks we are looking for variables to combine called a,b,c and d. ```{r, eval=FALSE} ``` We might want to get a list of all the objects we've created at this point. In R, the function ls() returns a character vector with the names of all the objects in a specified environment (by default, the set of user-defined objects and functions). ```{r} ls() ``` ## Getting Help R has very good built-in documentation that describes what functions do. To get help about a particular function, use ? followed by the function name, like so: ?ls If you are not exactly sure of the function name, you can perform a search: ??mean We can manipulate objects like so: ```{r} ``` Note that the value of ```x``` is not modified here. We did not save the output to a new object, so it is printed to the screen. If we want to update the value of ```x```, we need to use our assignment operator: ```{r} ``` R handles vector math for us automatically: ```{r} ``` > **Vector exercises:** > >1. Create a new vector, (called nums), that contains any 5 numbers you like > >2. There are a number of functions that operate on vectors, including length(), max(), min(), and mean(), all of which do exactly what they sound like they do. So for example length(nums) should return 5, because nums is a 5-element vector. Use these functions to get the minimum, maximum, and mean value for the nums vector you created. ```{r} ``` ## Object Types All objects have a type. Object types are a complex topic and we are only going to scratch the surface today. To slightly simplify, all data objects in R are either atomic vectors (contain only a single type of data), or data structures that combine atomic vectors in various ways. We'll walk through a few examples. First, let's consider a couple of the vectors that we've already made. ```{r} ``` *Numeric* and *character* data types are two of the most common we'll encounter, and are just what they sound like. Another useful type is *logical* data, as in TRUE/FALSE. We can create a logical vector directly like so: ```{r} ``` Or we can use logical tests. ```{r} ``` Note that the logical test here (>2) is applied independently to each element in the vector. We'll come back to this when we talk about data subsets. > **Object type exercises:** > >1. Construct a logical vector with the same number of elements as your nums vector, that is TRUE if the corresponding element in the nums vector is less than the mean of the nums vector, and FALSE otherwise. Call this vector big_nums ```{r} ``` ## Subsetting and Logical Vectors We can also extract portions of vectors that we've created using ```[]``` following the vector, with the positions we wish to extract. For example, we can extract portions of the nums vector: ```{r} ``` Now it can be useful to specify which positions you would like to include, but often it is more useful to create new objects based on some sort of rule. That is where logical vectors come in, and really how you will mostly be using logical vectors going forward. For example, in the last exercise, we constructed a logical vector that was the same length as our nums vector that was ```TRUE``` with if the corresponding element in the nums vector is less than the mean of the nums vector, and ```FALSE``` otherwise. Let's take a look at both of these vectors again. ```{r} ``` Let's say we actually want to extract all of the numbers that are bigger than the mean (so all of the ```TRUE```) values. All we have to do is put the logical vector in brackets. ```{r} ``` We don't necessarily have to define this vector ahead of time, but can put the rule in the brackets as well. ```{r} ``` > **Vector Subsetting Data exercises:** > > 1. Subset nums for all numbers that are larger than the minimum. > > Hints -- logical tests in R - greater than, less than, equal to, not equal to > > > '> , < , == , !=' ```{r} ``` ## Data Frames So far we've been talking about atomic vectors, which only contain a single data type (every element is logical, or character, or numeric). However, data sets will usually have multiple different data types: numeric for continunous data, character for categorical data and sample labels. Depending on how underlying types are combined, we can have four different "higher-level" data types in R: **Dimensions** | **Homogeneous** | **Heterogeneous** ---------------|-----------------|------------------- 1-D | atomic vector | list 2-D | matrix | data frame / tibble We'll focus on data frames for today, but lists and matrices can also be very powerful. A data frame is a collection of vectors, which can be (but don't have to be) different types, but all have to have the same length. Let's make a couple of toy data frames. One way to do this is with the data.frame() function. ```{r} ``` str() gives lots of information about the data type of the constituent parts of a data frame ```{r} ``` We can use the function head() to look at part of a a dataframe (or any R object). This can be very useful if you have a very large or long dataframe. You also can control how many lines of the dataframe you view with ```n=#```, although the default is 6. ```{r} ``` The summary() function can also be very useful to get a snapshot of the data in your dataframe. ```{r} ``` ###Reading files into R So far we have been working with small objects we created by hand. A more common way to create dataframes is by reading from a file. There are a few functions to do this in R: read.table() and read.csv() make this easy. ```{r} ``` > **File reading exercise** > >1. Try using head() to see the first 20 rows and summary() to see some information about the data. ```{r} ``` Again, summary() is a good way to see some basic information about a dataframe ```{r} ``` The view function can be useful for viewing dataframes in a spread-sheet like format. ```{r, eval=FALSE} ``` ### Subsetting and Manipulating Dataframes We learned about how to subset vectors earlier, now let's look at ways to get subsets of data from two-dimensional data. R uses ```[]``` to index rows and columns, like so: ```{r, eval=FALSE} ``` The first number refers to rows, and the second to columns in the case for 2-dimensional objects. Notice that when you are extracting data from a single column, the data are returned as a vector of the same type as the column. However, if you extract more than one column, the data are returend as a data frame. ```{r, eval=FALSE} ``` Often we want to select a set of rows from a data frame matching some criteria. We can use the subset() function for this. Let's start by just getting the data for key bus routes. ```{r} ``` Sometimes we might want something more complicated. Let's say we want to get data for all "local" bus routes with at least 1000 daily riders. ```{r} ``` > **Subsetting Data exercises:** > > 1. Subset bus when ridership is greater than 2500. > > 2. Subset bus when bus routes are NOT classified as Key. > > 3. Subset bus using the conditions of both #1 and #2, save as bus_2500_notKey > > Hints -- logical tests in R > > > '> , < , == , !=' > > > '& is and -->> z <- x & y TRUE only when both x and y are true' > > > '| is or -->> z <- x | y TRUE if either x or y is true' > ```{r} ``` We can add and manipulate the data frame as well, but we will save that for the next session, and use some packages specifically built for that purpose. ## Writing Data We've added several variables now, and we might want to write our updated dataset to a file. We do this with the write.table() function, which writes out a data.frame. ```{r} ``` But where did R put the file on our computer? R does all file operations without a full path in a working directory. RStudio has a preference to set the default working directory, which is typically something like /Users/Danielle/R. To see the current working directory, use: ```{r} getwd() ``` We can change the working directory with ```setwd()```. >**File operators exercises:** > >1. Write a copy of bus_2500_notKey, but **be sure to give it a different name than the original!** Otherwise you will overwrite your original data. > ```{r} ``` ================================================ FILE: intro-to-r/instructor_copy.Rmd ================================================ --- title: "Intro to R" subtitle: "Bioinformatics Coffee Hour" date: "Augus 4, 2020" author: "Danielle Khost" output: html_document --- ##Setting up our working directory and saving objects vs files R is a functional programming language, which means that most of what one does is apply functions to objects. We will begin with a very brief introduction to R objects and how functions work, and then focus on getting data into R, manipulating that data in R, plotting, and analysis. First, we need to make sure we are working out of the correct directory. You can see the working directory above the console in RStudio, or use the `getwd()` function to see the current working directory. If you are not currently working in a good location to read and save files, you can use `setwd()` to change the working directory to the location of your choice. ```{r} getwd() ``` ## Vectors Let's start by creating one of the simplest R objects, a vector of numbers. ```{r} v1<-c(1,2,3,4,5) ``` v1 is an *object* which we created by using the ```<-``` operator (less followed by a dash). v1 now contains the output of the *function* ```c(1,2,3,4,5)``` (c, for **c**ombined, combines elements into a vector Let's display the contents of v1: ```{r} print(v1) ``` We can also just type the name of an object (in this case ```v1```) to display it. Let's make a few more objects: ```{r} x<-10 x y<-11 y some_letters<-c("a", "b", "c", "d") ``` With this last variable, we had to use ```""``` to specify that we want to create a character vector. Otherwise it thinks we are looking for variables to combine called a,b,c and d. ```{r, eval=FALSE} some_letters <- c(a,b,c,d) ``` We might want to get a list of all the objects we've created at this point. In R, the function ls() returns a character vector with the names of all the objects in a specified environment (by default, the set of user-defined objects and functions). ```{r} ls() ``` ## Getting Help R has very good built-in documentation that describes what functions do. To get help about a particular function, use ? followed by the function name, like so: ?ls If you are not exactly sure of the function name, you can perform a search: ??mean We can manipulate objects like so: ```{r} x+5 x*2 x ``` Note that the value of ```x``` is not modified here. We did not save the output to a new object, so it is printed to the screen. If we want to update the value of ```x```, we need to use our assignment operator: ```{r} x<-x+5 x ``` R handles vector math for us automatically: ```{r} v1*x v2<-v1*x v2 ``` > **Vector exercises:** > >1. Create a new vector, (called nums), that contains any 5 numbers you like > >2. There are a number of functions that operate on vectors, including length(), max(), min(), and mean(), all of which do exactly what they sound like they do. So for example length(nums) should return 5, because nums is a 5-element vector. Use these functions to get the minimum, maximum, and mean value for the nums vector you created. ```{r} #1 nums <- c(33, 22, 41, 54, 91) #2 length(nums) min(nums) max(nums) mean(nums) ``` ## Object Types All objects have a type. Object types are a complex topic and we are only going to scratch the surface today. To slightly simplify, all data objects in R are either atomic vectors (contain only a single type of data), or data structures that combine atomic vectors in various ways. We'll walk through a few examples. First, let's consider a couple of the vectors that we've already made. ```{r} nums <- c(33, 22, 41, 54, 91) class(nums) class(some_letters) ``` *Numeric* and *character* data types are two of the most common we'll encounter, and are just what they sound like. Another useful type is *logical* data, as in TRUE/FALSE. We can create a logical vector directly like so: ```{r} logic1<-c(TRUE, TRUE, FALSE, FALSE) logic1 class(logic1) ``` Or we can use logical tests. ```{r} logic2<-v1>2 logic2 ``` Note that the logical test here (>2) is applied independently to each element in the vector. We'll come back to this when we talk about data subsets. > **Object type exercises:** > >1. Construct a logical vector with the same number of elements as your nums vector, that is TRUE if the corresponding element in the nums vector is less than the mean of the nums vector, and FALSE otherwise. Call this vector big_nums ```{r} #1 big_nums <- nums>mean(nums) ``` ## Subsetting and Logical Vectors We can also extract portions of vectors that we've created using ```[]``` following the vector, with the positions we wish to extract. For example, we can extract portions of the nums vector: ```{r} nums[1] nums[1:5] nums[c(1,4,5)] ``` Now it can be useful to specify which positions you would like to include, but often it is more useful to create new objects based on some sort of rule. That is where logical vectors come in, and really how you will mostly be using logical vectors going forward. For example, in the last exercise, we constructed a logical vector that was the same length as our nums vector that was ```TRUE``` with if the corresponding element in the nums vector is less than the mean of the nums vector, and ```FALSE``` otherwise. Let's take a look at both of these vectors again. ```{r} nums big_nums ``` Let's say we actually want to extract all of the numbers that are bigger than the mean (so all of the ```TRUE```) values. All we have to do is put the logical vector in brackets. ```{r} nums[big_nums] ``` We don't necessarily have to define this vector ahead of time, but can put the rule in the brackets as well. ```{r} nums[nums>mean(nums)] ``` > **Vector Subsetting Data exercises:** > > 1. Subset nums for all numbers that are larger than the minimum. > > Hints -- logical tests in R - greater than, less than, equal to, not equal to > > > '> , < , == , !=' ```{r} #1 nums[nums>min(nums)] ``` ## Data Frames So far we've been talking about atomic vectors, which only contain a single data type (every element is logical, or character, or numeric). However, data sets will usually have multiple different data types: numeric for continunous data, character for categorical data and sample labels. Depending on how underlying types are combined, we can have four different "higher-level" data types in R: **Dimensions** | **Homogeneous** | **Heterogeneous** ---------------|-----------------|------------------- 1-D | atomic vector | list 2-D | matrix | data frame / tibble We'll focus on data frames for today, but lists and matrices can also be very powerful. A data frame is a collection of vectors, which can be (but don't have to be) different types, but all have to have the same length. Let's make a couple of toy data frames. One way to do this is with the data.frame() function. ```{r} df1<-data.frame(label=c("rep1", "rep2", "rep3", "rep4"), data=c(23, 34, 15, 19)) df1 class(df1) ``` str() gives lots of information about the data type of the consituent parts of a data frame ```{r} str(df1) ``` We can use the function head() to look at part of a a dataframe (or any R object). This can be very useful if you have a very large or long dataframe. You also can control how many lines of the dataframe you view with ```n=#```, although the default is 6. ```{r} head(df1) head(df1, n=2) ``` The summary() function can also be very useful to get a snapshot of the data in your dataframe. ```{r} summary(df1) ``` ###Reading files into R So far we have been working with small objects we created by hand. A more common way to create dataframes is by reading from a file. There are a few functions to do this in R: read.table() and read.csv() make this easy. ```{r} bus<-read.table(file="/home/rstudio/data/mbta_bus.tsv", sep="\t", header=TRUE) ``` > **File reading exercise** > >1. Try using head() to see the first 20 rows and summary() to see some information about the data. ```{r} #2 head(bus, n=20) ``` Again, summary() is a good way to see some basic information about a dataframe ```{r} summary(bus) ``` The view function can be useful for viewing dataframes in a spread-sheet like format. ```{r, eval=FALSE} View(bus) ``` ### Subsetting and Manipulating Dataframes We learned about how to subset vectors earlier, now let's look at ways to get subsets of data from two-dimensional data. R uses ```[]``` to index rows and columns, like so: ```{r, eval=FALSE} bus[1,1] bus[1,] bus[1:5,] bus[1:5,4] bus[c(1,3,5),c(2,3)] ``` The first number refers to rows, and the second to columns in the case for 2-dimensional objects. Notice that when you are extracting data from a single column, the data are returned as a vector of the same type as the column. However, if you extract more than one column, the data are returend as a data frame. ```{r, eval=FALSE} str(bus[1,1]) str(bus[1:5,3]) str(bus[1:3,1:3]) ``` Often we want to select a set of rows from a data frame matching some criteria. We can use the subset() function for this. Let's start by just getting the data for key bus routes. ```{r} key.bus<-subset(bus,type=="Key") View(key.bus) ``` Sometimes we might want something more complicated. Let's say we want to get data for all "local" bus routes with at least 1000 daily riders. ```{r} local.bus<-subset(bus, type=="Local" & ridership > 1000) View(local.bus) ``` > **Subsetting Data exercises:** > > 1. Subset bus when ridership is greater than 2500. > > 2. Subset bus when bus routes are NOT classified as Key. > > 3. Subset bus using the conditions of both #1 and #2, save as bus_2500_notKey > > Hints -- logical tests in R > > > '> , < , == , !=' > > > '& is and -->> z <- x & y TRUE only when both x and y are true' > > > '| is or -->> z <- x | y TRUE if either x or y is true' > ```{r} #1 subset(bus,ridership>2500) #2 subset(bus,type!="Key") #3 bus_2500_notKey <- subset(bus, ridership>2500 & type != "Key") ``` We can add and manipulate the data frame as well, but we will save that for the next session, and use some packages specifically built for that purpose. ## Writing Data We've added several variables now, and we might want to write our updated dataset to a file. We do this with the write.table() function, which writes out a data.frame. ```{r} write.table(bus, file="mbta_bus_data.tsv", quote=F, sep="\t", row.names=F, col.names=T) ``` But where did R put the file on our computer? R does all file operations without a full path in a working directory. RStudio has a preference to set the default working directory, which is typically something like /Users/Danielle/R. To see the current working directory, use: ```{r} getwd() ``` We can change the working directory with ```setwd()```. >**File operators exercises:** > >1. Write a copy of bus_2500_notKey, but **be sure to give it a different name than the original!** Otherwise you will overwrite your original data. > ```{r} write.table(bus_2500_notKey,file="bus_2500_notKey.tsv",quote=F,sep="\t",row.names=F,col.names=F) ``` ================================================ FILE: intro_awk_spring2021/.ipynb_checkpoints/index-checkpoint.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "## Manipulating files with AWK\n", "### Bioinformatics Coffee Hour - April 14, 2020\n", "\n", "### What is awk?\n", "Invented in the 1970's, [awk](https://en.wikipedia.org/wiki/AWK) is a scripting language included in most Unix-like operating systems. It specializes in one-liner programs and manipulating text files.\n", "\n", "In many cases, if you're parsing information from a text file (such as a [BED](https://en.wikipedia.org/wiki/BED_(file_format)) file, [FASTA](https://en.wikipedia.org/wiki/FASTA) file, etc.), you could write a Python script...or you could do it with awk in a single line!\n", "\n", "### Syntax\n", "awk scripts are organized as:\n", "\n", "`awk 'pattern { action; other action }' file`\n", "\n", "Meaning that every time that the pattern is true, awk will execute the action in the brackets.\n", "If no pattern is specified, the action will be taken for every line in the input file, e.g. the following command prints every line:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "The two most important patterns are `BEGIN` and `END`, which tell the action to take place before any lines are read and after the last line." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{sum=0} {sum+=1} END {print sum}' data/hg38.genome" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ " The above line sets a variable at the start of the script, adds 1 to it every line, then prints its value at the end." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "If a variable hasn't been initialized, it is treated as 0 in numeric expressions, and an empty string in string expressions—awk will not print an error!\n", "So the following awk script also prints the number of lines in the file data/hg38.genome:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{sum+=1} END {print sum}' data/hg38.genome" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Input and output\n", "Input to awk is split into **records** and **fields**.\n", "- By default, **records** are separated by newline character, i.e # of records = # of lines in input file\n", "- Each record is subdivided into **fields**, i.e. columns, as determined by the field separator (see below)\n", "\n", "There are several important built-in variable in awk.\n", "The fields (columns) of each record are referred to by `$number`, so the first column would be `$1`, second would be `$2`, etc. `$0` refers to the entire record.\n", "\n", "So to print the second column of each line in the file, we'd use:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print $2}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "And if we wanted to print the second then the first:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print $2,$1}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note that when the different fields are separated with commas in the `print` statement, they are joined by the output field separator (the **OFS** variable, described below), which is by default a space.\n", "If the comma is omitted between fields (e.g., `awk '{print $2 $1}'`, they are concatenated without a separator.\n", "\n", "We can also print strings using using quotation marks:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print \"First column:\" $1}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Which for every line of the file will print the text \"First column:\" followed by the value in the first field." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "---\n", "awk has several other built-in variables that are very useful for parsing text, including:\n", "\n", "| | |\n", "---|---|\n", "| **FS** | field separator (default: white space) |\n", "| **OFS** | output field separator, i.e. what character separates fields when printing|\n", "| **RS** | record separator, i.e. what character records are split on (default: new line) |\n", "| **ORS** | output record separator |\n", "| **NR** | number of records in input (# lines by default) |\n", "\n", "Assigning to a field causes the entire record ($0) to be recomputed using **OFS**. We can use this to convert between file formats, e.g. make a comma-separated text file into a tab-separated file. First, let's look at the first few lines on our comma-separated file using `head`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/enhancers.csv" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now let's convert the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\",\" ; OFS=\"\\t\"} {$1 = $1; print $0}' data/enhancers.csv | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Conditionals and pattern matching\n", "Like other programming languages, awk allows conditional matching with if/else statements.\n", "\n", "`awk '{if(condition) action; else other_action}'`\n", "\n", "awk uses the following conditional operators:\n", "\n", "| | |\n", "|-|-|\n", "|==|equal to|\n", "|!=|not equal to|\n", "|>|greater than|\n", "|>=|greater than or equal to|\n", "|<|less than|\n", "|<=|less than or equal to|\n", "|&&|AND|\n", "| \\|\\| |OR|\n", "| ! | NOT |\n", "\n", "In addition, awk also supports string matching using regular expressions, using the following expressions:\n", "\n", "| | |\n", "|-|-|\n", "|\\~|matches|\n", "|!~|does not match|\n", "\n", "For string matching, the pattern being matched must be enclosed by slashes, like so:\n", "\n", "`awk '{if($1 ~ /pattern/) print}'`\n", "\n", "Note that if an action isn't specified, the default action is `{print}`, so the previous awk command is equivalent to the following, which specifies only a pattern expression:\n", "\n", "`awk '$1 ~ /pattern/'`\n", "\n", "---\n", "### Example uses:\n", "\n", "- Count number of sequences in a FASTQ file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'END{print NR/4}' data/example.fastq" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: this is technically safer than using grep, as you don't have to worry about accidentally counting the quality line.\n", "\n", "- Only print annotations on a specific scaffold (chr1) that fall between 1Mb and 2Mb from a BED annotation file. First let's look at the first few lines of the file using `head`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now subset the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\";OFS=\"\\t\"} {if($1 == \"chr1\" && $2 >=1000000 && $2 <= 2000000) print}' data/Homo_sapiens_ucscGenes.subset.bed | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: when we specify that we only want annotations from chr1, we're using exact match (`== \"chr1\"`) and not pattern match (`~ /chr1/`)...why is this??" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Only print lines of GFF annotation file that match the string \"exon\" in their third column. Again, let's look at the first few lines of the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/Homo_sapiens.GRCh38.subset.gff3" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Then subset the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"} {if($3 ~ /exon/) print $0}' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Convert from GFF (genome feature file) to BED file " ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep -v '^#' data/Homo_sapiens.GRCh38.subset.gff3 | awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} {print $1,$4-1,$5}' | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: remember that BED and GFF files have different coordinate systems, i.e. BED start coordinate is 0 based, half-open, GFF is 1-based inclusive! Also, we are first using grep to skip the header lines in the GFF file.\n", "\n", "Alternatively, you could do the whole thing with only awk, no grep required:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} !/^#/ {print $1,$4-1,$5}' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "With this command, `!/^#/` is a pattern (like `BEGIN` or `END`) that tell awk to execute the print statement when the start of the line does not match a `#`. Use whichever makes the most sense to you!\n", "\n", "### Practice\n", "Using awk:\n", "\n", "* Pull out only the CDS annotations (i.e. has \"CDS\" in the 3rd column) from the GFF file data/Homo_sapiens.GRCh38.subset.gff3 and output them in BED format" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Try it*" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '...' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Solution*" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "jupyter": { "source_hidden": true } }, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} {if($3 ~ /CDS/) print $1,$4-1,$5}' data/Homo_sapiens.GRCh38.subset.gff3" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Calculate the average length of gene annotations from the file data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Try it*" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '...' data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Solution*" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "jupyter": { "source_hidden": true } }, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; sum=0} {len=$3-$2; sum=sum+len} END{print sum/NR}' data/Homo_sapiens_ucscGenes.subset.bed" ] } ], "metadata": { "kernelspec": { "display_name": "Python 3", "language": "python", "name": "python3" }, "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.9.1" } }, "nbformat": 4, "nbformat_minor": 4 } ================================================ FILE: intro_awk_spring2021/binder/.ipynb_checkpoints/environment-checkpoint.yml ================================================ dependencies: - bash_kernel ================================================ FILE: intro_awk_spring2021/binder/environment.yml ================================================ dependencies: - bash_kernel ================================================ FILE: intro_awk_spring2021/data/.ipynb_checkpoints/Homo_sapiens.GRCh38.subset-checkpoint.gff3 ================================================ ##gff-version 3 ##sequence-region 1 1 248956422 1 Ensembl chromosome 1 248956422 . . . ID=chromosome:1 1 . biological_region 10469 11240 1.3e+03 . . external_name=oe %3D 0.79 1 . biological_region 10650 10657 0.999 + . logic_name=eponine 1 . biological_region 10655 10657 0.999 - . logic_name=eponine 1 . biological_region 10678 10687 0.999 + . logic_name=eponine 1 . biological_region 10681 10688 0.999 - . logic_name=eponine 1 . biological_region 10707 10716 0.999 + . logic_name=eponine 1 . biological_region 10708 10718 0.999 - . logic_name=eponine 1 . biological_region 10735 10747 0.999 - . logic_name=eponine 1 . biological_region 10737 10744 0.999 + . logic_name=eponine 1 . biological_region 10766 10773 0.999 + . logic_name=eponine 1 . biological_region 10770 10779 0.999 - . logic_name=eponine 1 . biological_region 10796 10801 0.999 + . logic_name=eponine 1 . biological_region 10810 10819 0.999 - . logic_name=eponine 1 . biological_region 10870 10872 0.999 + . logic_name=eponine 1 . biological_region 10889 10893 0.999 - . logic_name=eponine 1 havana pseudogene 11869 14409 . + . ID=gene:ENSG00000223972 1 havana lnc_RNA 11869 14409 . + . ID=transcript:ENST00000456328 1 havana exon 11869 12227 . + . Parent=transcript:ENST00000456328 1 havana exon 12613 12721 . + . Parent=transcript:ENST00000456328 1 havana exon 13221 14409 . + . Parent=transcript:ENST00000456328 1 havana pseudogenic_transcript 12010 13670 . + . ID=transcript:ENST00000450305 1 havana exon 12010 12057 . + . Parent=transcript:ENST00000450305 1 havana exon 12179 12227 . + . Parent=transcript:ENST00000450305 1 havana exon 12613 12697 . + . Parent=transcript:ENST00000450305 1 havana exon 12975 13052 . + . Parent=transcript:ENST00000450305 1 havana exon 13221 13374 . + . Parent=transcript:ENST00000450305 1 havana exon 13453 13670 . + . Parent=transcript:ENST00000450305 1 havana pseudogene 14404 29570 . - . ID=gene:ENSG00000227232 1 havana pseudogenic_transcript 14404 29570 . - . ID=transcript:ENST00000488147 1 havana exon 14404 14501 . - . Parent=transcript:ENST00000488147 1 havana exon 15005 15038 . - . Parent=transcript:ENST00000488147 1 havana exon 15796 15947 . - . Parent=transcript:ENST00000488147 1 havana exon 16607 16765 . - . Parent=transcript:ENST00000488147 1 havana exon 16858 17055 . - . Parent=transcript:ENST00000488147 1 havana exon 17233 17368 . - . Parent=transcript:ENST00000488147 1 havana exon 17606 17742 . - . Parent=transcript:ENST00000488147 1 havana exon 17915 18061 . - . Parent=transcript:ENST00000488147 1 havana exon 18268 18366 . - . Parent=transcript:ENST00000488147 1 havana exon 24738 24891 . - . Parent=transcript:ENST00000488147 1 havana exon 29534 29570 . - . Parent=transcript:ENST00000488147 1 . biological_region 15796 16060 0.999 - . external_name=rank %3D 1 1 mirbase ncRNA_gene 17369 17436 . - . ID=gene:ENSG00000278267 1 mirbase miRNA 17369 17436 . - . ID=transcript:ENST00000619216 1 mirbase exon 17369 17436 . - . Parent=transcript:ENST00000619216 1 . biological_region 28736 29810 1.01e+03 . . external_name=oe %3D 0.88 1 . biological_region 29116 29118 0.999 + . logic_name=eponine 1 . biological_region 29127 29206 1 + . external_name=rank %3D 1 1 . biological_region 29321 29395 1 - . external_name=rank %3D 1 1 . biological_region 29394 29396 0.999 - . logic_name=eponine 1 . biological_region 29448 29451 0.999 + . logic_name=eponine 1 havana ncRNA_gene 29554 31109 . + . ID=gene:ENSG00000243485 1 havana lnc_RNA 29554 31097 . + . ID=transcript:ENST00000473358 1 havana exon 29554 30039 . + . Parent=transcript:ENST00000473358 1 havana exon 30564 30667 . + . Parent=transcript:ENST00000473358 1 havana exon 30976 31097 . + . Parent=transcript:ENST00000473358 1 havana lnc_RNA 30267 31109 . + . ID=transcript:ENST00000469289 1 havana exon 30267 30667 . + . Parent=transcript:ENST00000469289 1 havana exon 30976 31109 . + . Parent=transcript:ENST00000469289 1 . biological_region 29583 29584 0.999 - . logic_name=eponine 1 mirbase ncRNA_gene 30366 30503 . + . ID=gene:ENSG00000284332 1 mirbase miRNA 30366 30503 . + . ID=transcript:ENST00000607096 1 mirbase exon 30366 30503 . + . Parent=transcript:ENST00000607096 1 havana ncRNA_gene 34554 36081 . - . ID=gene:ENSG00000237613 1 havana lnc_RNA 34554 36081 . - . ID=transcript:ENST00000417324 1 havana exon 34554 35174 . - . Parent=transcript:ENST00000417324 1 havana exon 35277 35481 . - . Parent=transcript:ENST00000417324 1 havana exon 35721 36081 . - . Parent=transcript:ENST00000417324 1 havana lnc_RNA 35245 36073 . - . ID=transcript:ENST00000461467 1 havana exon 35245 35481 . - . Parent=transcript:ENST00000461467 1 havana exon 35721 36073 . - . Parent=transcript:ENST00000461467 1 . biological_region 35904 36086 0.879 - . external_name=rank %3D 1 1 havana pseudogene 52473 53312 . + . ID=gene:ENSG00000268020 1 havana pseudogenic_transcript 52473 53312 . + . ID=transcript:ENST00000606857 1 havana exon 52473 53312 . + . Parent=transcript:ENST00000606857 1 havana pseudogene 57598 64116 . + . ID=gene:ENSG00000240361 1 havana lnc_RNA 57598 64116 . + . ID=transcript:ENST00000642116 1 havana exon 57598 57653 . + . Parent=transcript:ENST00000642116 1 havana exon 58700 58856 . + . Parent=transcript:ENST00000642116 1 havana exon 62916 64116 . + . Parent=transcript:ENST00000642116 1 havana pseudogenic_transcript 62949 63887 . + . ID=transcript:ENST00000492842 1 havana exon 62949 63887 . + . Parent=transcript:ENST00000492842 1 ensembl_havana gene 65419 71585 . + . ID=gene:ENSG00000186092 1 havana mRNA 65419 71585 . + . ID=transcript:ENST00000641515 1 havana exon 65419 65433 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 65419 65433 . + . Parent=transcript:ENST00000641515 1 havana exon 65520 65573 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 65520 65573 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 69037 69090 . + . Parent=transcript:ENST00000641515 1 havana exon 69037 71585 . + . Parent=transcript:ENST00000641515 1 havana CDS 69091 70008 . + 0 ID=CDS:ENSP00000493376 1 havana three_prime_UTR 70009 71585 . + . Parent=transcript:ENST00000641515 1 ensembl mRNA 69055 70108 . + . ID=transcript:ENST00000335137 1 ensembl five_prime_UTR 69055 69090 . + . Parent=transcript:ENST00000335137 1 ensembl exon 69055 70108 . + . Parent=transcript:ENST00000335137 1 ensembl CDS 69091 70008 . + 0 ID=CDS:ENSP00000334393 1 ensembl three_prime_UTR 70009 70108 . + . Parent=transcript:ENST00000335137 1 ensembl_havana ncRNA_gene 89295 133723 . - . ID=gene:ENSG00000238009 1 havana lnc_RNA 89295 120932 . - . ID=transcript:ENST00000466430 1 havana exon 89295 91629 . - . Parent=transcript:ENST00000466430 ================================================ FILE: intro_awk_spring2021/data/.ipynb_checkpoints/Homo_sapiens_ucscGenes.subset-checkpoint.bed ================================================ chr1 11873 14409 uc010nxq.1 chr1 14361 19759 uc009viu.3 chr1 14406 29370 uc009viw.2 chr1 34610 36081 uc001aak.3 chr1 69090 70008 uc001aal.1 chr1 134772 140566 uc021oeg.2 chr1 321083 321115 uc001aaq.2 chr1 321145 321207 uc001aar.2 chr1 322036 326938 uc009vjk.2 chr1 327545 328439 uc021oei.1 chr1 367658 368597 uc010nxu.2 chr1 420205 421839 uc001aax.1 chr1 566092 566115 uc021oej.1 chr1 566134 566155 uc021oek.1 chr1 566239 566263 uc021oel.1 chr1 568843 568913 uc001abb.3 chr1 621095 622034 uc010nxv.2 chr1 661138 670994 uc009vjm.3 chr1 668417 668479 uc001abi.2 chr1 668509 668541 uc001abj.3 chr1 671823 671885 uc010nxw.2 chr1 671915 671947 uc001abl.3 chr1 674239 679736 uc001abm.2 chr1 700244 714068 uc001abo.3 chr1 761585 762902 uc010nxx.2 chr1 762970 794826 uc001abp.2 chr1 803450 812182 uc001abt.4 chr1 846814 850328 uc001abu.1 chr1 852952 854817 uc010nxy.1 chr1 861120 879961 uc001abw.1 chr1 879582 894679 uc001abz.4 chr1 895966 901099 uc001aca.2 chr1 901876 910484 uc001acd.3 chr1 910578 917473 uc001ach.2 chr1 934341 935552 uc001aci.2 chr1 948846 949919 uc001acj.4 chr1 955502 991499 uc001ack.2 chr1 995116 1001833 uc001acl.1 chr1 1007125 1009687 uc021oen.1 chr1 1017197 1051736 uc001acu.2 chr1 1072396 1079434 uc001acv.3 chr1 1102483 1102578 uc001acw.2 chr1 1103242 1103332 uc010nye.1 chr1 1103295 1103317 uc031pkr.1 chr1 1104384 1104467 uc010nyf.1 chr1 1104434 1104456 uc031pks.1 chr1 1108435 1114935 uc001acx.1 chr1 1109285 1133313 uc001acy.2 chr1 1138887 1142089 uc001add.3 chr1 1146705 1149548 uc001ade.3 chr1 1152287 1167447 uc001adh.4 chr1 1167628 1170420 uc001adk.3 chr1 1177825 1182102 uc001adl.2 chr1 1189291 1209234 uc001ado.3 chr1 1215815 1227409 uc001adt.1 chr1 1227763 1243269 uc001aeb.2 chr1 1243993 1247057 uc001aed.3 chr1 1246964 1260067 uc001aee.2 chr1 1260142 1264276 uc001aeo.3 chr1 1266725 1269844 uc010nyk.2 chr1 1270657 1284492 uc001aer.4 chr1 1288070 1293915 uc001aew.3 chr1 1309109 1310562 uc009vkb.1 chr1 1321090 1334718 uc001afi.2 chr1 1334909 1337426 uc001afm.3 chr1 1337275 1342693 uc001afo.4 chr1 1353799 1356824 uc010nyo.2 chr1 1361507 1363167 uc010nyp.2 chr1 1370902 1378262 uc001afs.3 chr1 1385068 1405538 uc001aft.2 chr1 1407163 1431582 uc001afv.3 chr1 1447522 1470067 uc001afz.2 chr1 1470157 1475740 uc009vkf.3 chr1 1477052 1510262 uc001agd.3 chr1 1510354 1510644 uc021oer.1 chr1 1533387 1535476 uc021oes.1 chr1 1535818 1543166 uc001agf.1 chr1 1550794 1565990 uc001agg.3 chr1 1567559 1570030 uc001agp.3 chr1 1571099 1655775 uc001agv.1 chr1 1586822 1590469 uc001ahc.1 chr1 1592938 1624243 uc001ahg.4 chr1 1631377 1633247 uc001ahi.1 chr1 1656053 1663343 uc001ahx.2 chr1 1658823 1677438 uc001aia.2 chr1 1682670 1711508 uc001aie.3 chr1 1716724 1822526 uc001aif.3 chr1 1846265 1848733 uc001aih.1 chr1 1849028 1850740 uc001aij.2 chr1 1853395 1858842 uc001aik.3 chr1 1884751 1935276 uc001aim.1 chr1 1944651 1946969 uc001aio.1 chr1 1950767 1962192 uc001aip.2 chr1 1981908 2116834 uc001aiq.3 chr1 2112574 2114663 uc001aiu.1 chr1 2115898 2126214 uc031pkt.1 chr1 2121236 2123179 uc001aiz.2 chr1 2160133 2241652 uc001aja.4 chr1 2252695 2322993 uc001ajb.1 chr1 2281852 2284100 uc001ajc.3 chr1 2309493 2322993 uc010nyy.2 chr1 2323213 2336885 uc001aje.2 chr1 2336240 2344010 uc001ajg.3 chr1 2407753 2436964 uc001aji.1 chr1 2439974 2458035 uc001ajm.1 chr1 2460183 2461684 uc001ajn.3 chr1 2481358 2484284 uc001ajo.2 chr1 2486162 2488450 uc021oev.1 chr1 2487804 2495188 uc001ajt.1 chr1 2487804 2495267 uc001ajr.3 chr1 2518188 2522908 uc001ajv.2 chr1 2522080 2564481 uc001ajy.2 chr1 2572806 2706230 uc021oey.1 chr1 2938045 2939467 uc001ajz.3 chr1 2976180 2980350 uc001aka.3 chr1 2980635 2984289 uc010nzg.1 chr1 2985741 3355185 uc001akf.3 chr1 3044538 3044599 uc021oez.1 chr1 3371146 3397677 uc001akg.4 chr1 3404505 3528059 uc001akl.3 chr1 3477259 3477354 uc021ofa.1 chr1 3541555 3546694 uc001akm.3 chr1 3547330 3566671 uc001ako.3 chr1 3569128 3652765 uc001akp.3 chr1 3652547 3663937 uc009vlm.3 chr1 3668964 3688209 uc001akv.2 chr1 3689351 3692546 uc001akw.4 chr1 3696783 3713068 uc001akx.1 chr1 3728644 3773797 uc001aky.2 chr1 3773844 3801993 uc001alc.3 chr1 3805696 3816857 uc001alf.3 chr1 3816967 3832011 uc001alg.3 chr1 4000671 4012643 uc001ali.2 chr1 4472110 4484744 uc001alj.2 chr1 4715104 4843851 uc001aln.3 chr1 4847557 4852183 uc001alo.4 chr1 5621768 5728315 uc001alp.1 chr1 5624130 5624203 uc021ofm.1 chr1 5922731 5922801 uc021ofn.1 chr1 5922869 6052533 uc001alq.2 chr1 6105980 6161253 uc001aly.2 chr1 6161846 6240194 uc001amb.2 chr1 6245079 6259679 uc001amd.3 chr1 6266188 6281359 uc001amg.3 chr1 6281252 6296044 uc001amk.3 chr1 6297870 6299502 uc001amm.3 chr1 6304251 6305638 uc009vly.2 chr1 6307405 6321035 uc001amp.2 chr1 6324331 6453826 uc001amt.3 chr1 6475293 6479979 uc001amx.3 chr1 6484847 6521004 uc001amy.3 chr1 6489893 6489956 uc021ofo.1 chr1 6521213 6526255 uc001anh.3 chr1 6526151 6545529 uc010nzr.1 chr1 6581406 6614658 uc001ans.3 chr1 6615337 6639817 uc001ant.3 chr1 6640062 6649340 uc001anx.3 chr1 6650783 6662929 uc001aoa.3 chr1 6673755 6684093 uc001aob.4 chr1 6685209 6693642 uc001aod.3 chr1 6694227 6761966 uc001aof.2 chr1 6845383 7829766 uc001aoi.3 chr1 7831328 7841492 uc001aol.3 chr1 7844713 7905237 uc001aoo.3 chr1 7907671 7913565 uc001aos.3 chr1 7975930 8003225 uc001aot.3 chr1 7990338 7990408 uc021ofs.1 chr1 8021713 8045342 uc001aox.4 chr1 8071778 8086393 uc001aoz.3 chr1 8384389 8404227 uc001apb.3 chr1 8412463 8877699 uc001apf.3 chr1 8440651 8441235 uc001apg.1 chr1 8921058 8939151 uc001apj.2 chr1 8938893 8939943 uc021oft.1 chr1 9005892 9034503 uc031plc.1 chr1 9063358 9086404 uc009vmo.1 chr1 9097004 9129887 uc001apo.3 chr1 9164475 9189229 uc001apq.1 chr1 9186983 9189250 uc001aps.3 chr1 9208345 9242451 uc009vmq.3 chr1 9294862 9331394 uc001apt.3 chr1 9352940 9429590 uc010oae.2 chr1 9497727 9497837 uc021ofx.1 chr1 9599527 9642831 uc001apw.3 chr1 9648931 9674935 uc021ofy.1 chr1 9711789 9789172 uc001aqb.4 chr1 9712667 9714644 uc001aqc.4 chr1 9740902 9747627 uc021oga.1 chr1 9789078 9884550 uc001aqh.3 chr1 9908333 9970316 uc001aql.1 chr1 9989775 10002840 uc001aqm.3 chr1 10003485 10045556 uc001aqp.3 chr1 10057254 10076078 uc001aqq.3 chr1 10093040 10241296 uc001aqs.4 chr1 10270763 10441661 uc001aqw.4 chr1 10459084 10480201 uc001arc.3 chr1 10490158 10512060 uc021ogd.1 chr1 10520602 10532613 uc001arj.3 chr1 10535002 10690815 uc001arn.3 chr1 10696665 10856733 uc001aro.4 chr1 10744633 10744736 uc021ogh.1 chr1 11006529 11042094 uc010oao.2 chr1 11072678 11085549 uc001art.3 chr1 11086579 11107296 uc001aru.3 chr1 11114648 11120091 uc001arz.1 chr1 11126675 11159938 uc001asa.3 chr1 11166587 11322608 uc001asd.3 chr1 11203954 11209595 uc031plf.1 chr1 11249345 11256038 uc001ase.4 chr1 11333254 11348491 uc001asg.3 chr1 11539294 11597640 uc001ash.4 chr1 11708417 11714888 uc001asj.3 chr1 11714913 11723384 uc001asm.3 chr1 11724149 11734409 uc001aso.3 chr1 11734536 11751678 uc009vnc.3 chr1 11751780 11780336 uc001asr.1 chr1 11782186 11785914 uc001ass.2 chr1 11796141 11810828 uc001asv.3 chr1 11824461 11826573 uc001asy.1 chr1 11832138 11849642 uc001asz.3 chr1 11845786 11866160 uc001atc.2 chr1 11866152 11903201 uc001ate.5 chr1 11905766 11907840 uc001ati.3 chr1 11917520 11918992 uc001atj.3 chr1 11979644 11986485 uc001atk.3 chr1 11994723 12035599 uc001atm.3 chr1 12040237 12073572 uc009vni.3 chr1 12079298 12092106 uc001ato.2 chr1 12123433 12204264 uc001atq.3 chr1 12227059 12269277 uc001att.3 chr1 12251761 12251839 uc021ogi.1 chr1 12290112 12572098 uc001atv.3 chr1 12567299 12567451 uc001atz.1 chr1 12627938 12677820 uc001auc.3 chr1 12704565 12727097 uc001auf.3 chr1 12776117 12788726 uc009vnn.1 chr1 12806162 12821102 uc001auh.3 chr1 12834983 12838048 uc001aui.3 chr1 12851545 12856777 uc001auj.2 chr1 12884467 12891264 uc001auk.2 chr1 12907235 12908237 uc009vno.2 chr1 12916940 12921764 uc001aum.1 chr1 12939032 12946025 uc001aun.2 chr1 12952726 12958094 uc001auo.3 chr1 12976449 12980568 uc001aup.3 chr1 12998301 13007406 uc001auq.2 chr1 13035542 13038381 uc009vnq.1 chr1 13182959 13184326 uc010obg.2 chr1 13328195 13331692 uc001aut.1 chr1 13359818 13369057 uc001auu.1 chr1 13386646 13390765 uc001auv.3 chr1 13421175 13428191 uc001auw.1 chr1 13447413 13452656 uc010obi.1 chr1 13474052 13477569 uc009vnu.1 chr1 13495253 13498259 uc001aux.3 chr1 13516065 13526943 uc009vnv.1 chr1 13607430 13611550 uc001auy.2 chr1 13629937 13635299 uc001auz.4 chr1 13641972 13648988 uc001ava.1 chr1 13694888 13698405 uc009vny.1 chr1 13716087 13719064 uc009vnz.1 chr1 13736906 13747803 uc009voa.1 chr1 13801444 13840242 uc001avb.3 chr1 13910251 13944452 uc001avd.3 chr1 14031349 14151574 uc001avi.3 chr1 14925212 15444544 uc001avm.4 chr1 15438310 15478960 uc009voh.3 chr1 15480228 15546974 uc001avx.3 chr1 15573767 15724622 uc001awb.2 chr1 15653175 15670372 uc001awc.1 chr1 15736390 15756839 uc001awh.2 chr1 15764937 15773153 uc001awi.1 chr1 15783222 15798586 uc001awk.3 chr1 15802595 15817895 uc001awl.3 chr1 15817895 15850940 uc001awn.4 chr1 15853351 15898228 uc001aws.3 chr1 15898193 15911605 uc001awv.2 chr1 15943952 15987552 uc001awx.2 chr1 15986363 15988217 uc010obn.2 chr1 15992765 15995537 uc001awz.3 chr1 16010826 16061264 uc010obo.2 chr1 16062808 16067884 uc001axb.1 chr1 16068986 16074292 uc001axc.4 chr1 16085254 16113084 uc001axe.1 chr1 16133656 16134194 uc009vol.1 chr1 16160709 16174642 uc001axj.2 chr1 16174358 16266950 uc001axk.1 chr1 16268363 16302627 uc001axl.4 chr1 16317618 16317647 uc001axm.1 chr1 16330730 16333184 uc001axn.3 chr1 16340522 16345285 uc001axo.2 chr1 16348485 16360545 uc001axu.3 chr1 16384263 16400127 uc001axz.4 chr1 16450831 16482582 uc001aya.2 chr1 16524598 16539104 uc001ayc.1 chr1 16558181 16563659 uc001ayd.3 chr1 16576558 16678948 uc001ayg.3 chr1 16693524 16724643 uc001aym.5 chr1 16725137 16763919 uc001ayn.3 chr1 16767166 16786584 uc001ayq.3 chr1 16793930 16819196 uc001ayt.2 chr1 16847079 16847153 uc021ogp.1 chr1 16858892 16858966 uc021ogq.1 chr1 16860349 16862144 uc021ogr.1 chr1 16862254 16864669 uc001ayv.2 chr1 16872433 16872504 uc021ogs.1 chr1 16874159 16874232 uc021ogt.1 chr1 16875408 16875482 uc021ogu.1 chr1 16888921 16940100 uc001ayw.4 chr1 16944756 16959841 uc001azf.3 chr1 16972068 16976915 uc010och.2 chr1 17004765 17004836 uc021ogw.1 chr1 17006500 17006573 uc021ogx.1 chr1 17007749 17007823 uc021ogy.1 chr1 17017712 17046652 uc001azn.1 chr1 17052060 17052133 uc021ogz.1 chr1 17053779 17053850 uc021oha.1 chr1 17081128 17090975 uc010ock.3 chr1 17180899 17180971 uc021ohb.1 chr1 17185443 17185516 uc021ohc.1 chr1 17186692 17186765 uc021ohd.1 chr1 17188415 17188486 uc021ohe.1 chr1 17197439 17200574 uc021ohf.2 chr1 17201957 17202031 uc021ohg.1 chr1 17215040 17216161 uc001azs.1 chr1 17216171 17216245 uc021ohh.1 chr1 17222645 17222720 uc021ohi.1 chr1 17248444 17299474 uc001azt.2 chr1 17300998 17307173 uc001azw.3 chr1 17312452 17338423 uc001baa.2 chr1 17345224 17380665 uc001bae.3 chr1 17393255 17445948 uc001baf.3 chr1 17531620 17572501 uc001bah.1 chr1 17566189 17572501 uc009vpb.1 chr1 17575592 17610727 uc001bai.3 chr1 17581660 17581778 uc021ohk.1 chr1 17634689 17690495 uc001baj.2 chr1 17698740 17728195 uc001bak.1 chr1 17733250 17765059 uc001bal.3 chr1 17866329 18024370 uc001ban.3 chr1 18081807 18153558 uc001bat.3 chr1 18434239 18704977 uc001bau.2 chr1 18701063 18702174 uc001baw.1 chr1 18807423 18812480 uc001bax.3 chr1 18957499 19062632 uc001bay.3 chr1 19166092 19186155 uc001bba.1 chr1 19197923 19229293 uc001bbc.3 chr1 19209695 19209769 uc021ohm.2 chr1 19230773 19282826 uc001bbd.2 chr1 19400999 19536746 uc001bbi.3 chr1 19542157 19578053 uc001bbo.4 chr1 19578074 19586622 uc001bbs.3 chr1 19592475 19600568 uc021ohn.1 chr1 19609056 19615280 uc001bbv.1 chr1 19619740 19622230 uc021ohp.1 chr1 19629201 19638640 uc001bbw.3 chr1 19638739 19655794 uc001bby.3 chr1 19665266 19812066 uc021ohr.1 chr1 19673334 19675427 uc001bcf.2 chr1 19750877 19751182 uc021ohs.1 chr1 19923470 19956315 uc021ohu.1 chr1 19934300 19935138 uc021ohx.2 chr1 19969722 19984949 uc001bcj.2 chr1 19991779 20006055 uc001bcl.3 chr1 20008705 20126410 uc001bcn.3 chr1 20140521 20141771 uc001bcr.3 chr1 20208887 20239437 uc001bcs.4 chr1 20246799 20250110 uc001bct.1 chr1 20301923 20306932 uc010odb.2 chr1 20396700 20418394 uc001bcy.3 chr1 20439142 20446059 uc001bcz.4 chr1 20465822 20476879 uc009vpp.1 chr1 20490483 20501687 uc009vpq.1 chr1 20512577 20519942 uc001bdb.3 chr1 20617411 20681387 uc009vps.2 chr1 20686293 20755287 uc001bdf.2 chr1 20808883 20812728 uc001bdh.3 chr1 20825940 20834674 uc001bdi.4 chr1 20878931 20881513 uc001bdj.3 chr1 20915443 20945400 uc001bdk.3 chr1 20959947 20978004 uc001bdm.3 chr1 20978259 20988037 uc001bdo.1 chr1 20990506 21044317 uc001bdr.4 chr1 21046224 21059133 uc009vpy.1 chr1 21069170 21113181 uc001bdw.1 chr1 21132784 21503381 uc001bef.3 chr1 21543739 21616766 uc001bei.2 chr1 21602542 21604868 uc001ben.1 chr1 21619782 21626362 uc001beo.2 chr1 21749600 21754300 uc001bep.1 chr1 21761832 21762609 uc001beq.1 chr1 21766582 21811393 uc001ber.4 chr1 21835857 21904905 uc001bet.3 chr1 21922707 21978348 uc001bew.3 chr1 22004791 22109688 uc001bfb.3 chr1 22138757 22151714 uc001bfg.1 chr1 22148736 22263750 uc001bfj.3 chr1 22303417 22315847 uc001bfk.3 chr1 22351706 22357715 uc001bfm.4 chr1 22379119 22419436 uc001bfr.3 chr1 22443797 22469519 uc001bfs.4 chr1 22778343 22857650 uc001bfu.2 chr1 22890003 22930087 uc001bfx.1 chr1 22963117 22966175 uc001bfy.3 chr1 22970117 22974603 uc001bga.4 chr1 22979681 22988029 uc001bgd.3 chr1 23037330 23241823 uc001bge.3 chr1 23046009 23046091 uc021oib.1 chr1 23189651 23189719 uc021oic.1 chr1 23243782 23247347 uc001bgg.1 chr1 23337326 23342343 uc001bgh.1 chr1 23345940 23410184 uc001bgj.2 chr1 23370797 23370865 uc021oid.1 chr1 23410515 23495517 uc010odv.1 chr1 23490445 23490546 uc021oie.1 chr1 23518387 23521222 uc001bgn.3 chr1 23636275 23670853 uc001bgp.4 chr1 23685940 23694879 uc001bgt.3 chr1 23695463 23698330 uc001bgw.3 chr1 23707554 23751261 uc021oig.1 chr1 23755055 23810750 uc001bha.2 chr1 23801092 23803135 uc001bhd.4 chr1 23832919 23857712 uc001bhe.2 chr1 23853364 23855542 uc001bhf.1 chr1 23884420 23886285 uc001bhh.4 chr1 23907984 23967056 uc001bhi.3 chr1 24018268 24022915 uc001bhk.3 chr1 24069855 24088549 uc001bho.3 chr1 24086871 24104787 uc001bhp.2 chr1 24104875 24114722 uc001bhq.3 chr1 24117645 24122029 uc001bht.3 chr1 24122088 24126060 uc009vqo.1 chr1 24128366 24151949 uc001bib.3 chr1 24171571 24194859 uc001bie.3 chr1 24200459 24239817 uc001bif.3 chr1 24255559 24255637 uc021oik.1 chr1 24286300 24289949 uc001big.3 chr1 24295572 24306953 uc021oir.1 chr1 24320925 24320957 uc021oit.1 chr1 24382530 24438665 uc001bin.4 chr1 24446260 24469775 uc001biq.2 chr1 24480646 24513765 uc001bis.3 chr1 24526729 24538180 uc010oei.1 chr1 24578722 24578758 uc021oiu.1 chr1 24579767 24579803 uc021oiv.1 chr1 24645811 24690970 uc021oiw.1 chr1 24683488 24740262 uc001bjc.3 chr1 24742244 24799473 uc001bjh.3 chr1 24822822 24828850 uc021oiz.1 chr1 24829386 24863510 uc001bjj.3 chr1 24882566 24935818 uc001bjk.2 chr1 24969593 24999772 uc001bjm.3 chr1 25071759 25170815 uc001bjo.2 chr1 25226001 25256770 uc001bjq.3 chr1 25548766 25559013 uc001bjt.1 chr1 25568739 25573985 uc001bjw.3 chr1 25598980 25656936 uc001bjz.3 chr1 25629228 25631643 uc001bkd.1 chr1 25664788 25688852 uc001bke.3 chr1 25688739 25747363 uc001bkf.3 chr1 25757387 25826698 uc001bkk.3 chr1 25870075 25895377 uc001bkl.4 chr1 25943958 26111258 uc001bkm.2 chr1 26126666 26144713 uc021ojl.1 chr1 26146396 26159433 uc001bkq.4 chr1 26146444 26150097 uc010oeu.1 chr1 26160496 26185848 uc001bkw.1 chr1 26187974 26197744 uc001bkx.3 chr1 26210676 26232993 uc010oev.2 chr1 26286257 26324648 uc001bld.4 chr1 26348270 26362954 uc001blf.3 chr1 26364513 26372604 uc001blg.1 chr1 26377795 26394125 uc001bli.2 chr1 26438267 26452039 uc009vsb.3 chr1 26485510 26489119 uc001blk.3 chr1 26496387 26497364 uc001bll.4 chr1 26503980 26516375 uc001blm.4 chr1 26517118 26529033 uc010oez.2 chr1 26551810 26556331 uc001blq.3 chr1 26560692 26605299 uc001bls.1 chr1 26606212 26608013 uc001blu.3 chr1 26608772 26633195 uc001blw.3 chr1 26644410 26647014 uc001bmc.3 chr1 26648349 26680621 uc001bmd.4 chr1 26688124 26699266 uc009vsj.1 chr1 26737268 26756219 uc001bmj.3 chr1 26758772 26797795 uc001bmk.3 chr1 26789254 26794028 uc001bmo.1 chr1 26798901 26803133 uc001bmp.4 chr1 26872342 26901520 uc001bms.1 chr1 26881032 26881084 uc021ojp.1 chr1 27022521 27108601 uc001bmv.1 chr1 27114453 27124894 uc001bmz.3 chr1 27145536 27392034 uc021ojq.1 chr1 27153200 27182211 uc001bnb.3 chr1 27189632 27190947 uc001bnc.1 chr1 27189786 27190449 uc010ofi.1 chr1 27205872 27216869 uc001bnd.1 chr1 27216978 27226962 uc001bne.3 chr1 27237974 27240567 uc001bnf.3 chr1 27248212 27273362 uc001bng.2 chr1 27276046 27286901 uc001bni.2 chr1 27320194 27327377 uc001bnj.4 chr1 27331510 27339333 uc010ofj.2 chr1 27425299 27481621 uc001bnm.4 chr1 27533765 27533868 uc021ojs.1 chr1 27561006 27635124 uc009vst.3 chr1 27648635 27662891 uc001bnr.4 chr1 27650364 27653016 uc021oju.1 chr1 27668482 27680423 uc001bnw.2 chr1 27681669 27693337 uc001bny.1 chr1 27695600 27701315 uc001boa.3 chr1 27705595 27709805 uc001boc.3 chr1 27719147 27722317 uc001bod.4 chr1 27730733 27816678 uc001bof.2 chr1 27860755 27930143 uc009vsy.3 chr1 27938800 27961727 uc001bom.3 chr1 27992571 27998724 uc001bon.1 chr1 28052489 28089423 uc001bor.3 chr1 28099693 28150963 uc001bou.4 chr1 28157251 28178183 uc001bov.2 chr1 28160911 28161077 uc001boy.1 chr1 28199054 28213193 uc001bpc.4 chr1 28218048 28241236 uc001bpe.1 chr1 28261503 28285663 uc001bpg.3 chr1 28286503 28294604 uc001bph.1 chr1 28296854 28415148 uc001bpi.2 chr1 28473676 28503455 uc001bpl.3 chr1 28526789 28559542 uc001bpn.3 chr1 28562601 28564616 uc001bpq.3 chr1 28567542 28567564 uc021okb.1 chr1 28585962 28609002 uc001bps.3 chr1 28655512 28662478 uc009vtg.3 chr1 28764660 28826881 uc001bpy.3 chr1 28832454 28837404 uc001bqd.3 chr1 28844744 28865708 uc001bqf.2 chr1 28879528 28905057 uc001bqi.3 chr1 28905049 28908383 uc001bqo.3 chr1 28905254 28905334 uc001bqq.1 chr1 28919006 28921088 uc001bqv.3 chr1 28929608 28969604 uc001bqy.3 chr1 28975111 28975246 uc009vtj.1 chr1 28995239 29042115 uc001bra.3 chr1 29016176 29016306 uc021oke.1 chr1 29063132 29096287 uc021okf.1 chr1 29138653 29190208 uc001brf.1 chr1 29213602 29446558 uc001brm.2 chr1 29445936 29450421 uc001brn.2 chr1 29474249 29508637 uc001bro.3 chr1 29519384 29557454 uc001brq.1 chr1 29563027 29653325 uc001bru.3 chr1 30486798 30510456 uc001bry.3 chr1 31184123 31196432 uc001brz.3 chr1 31191618 31199593 uc001bsb.1 chr1 31205314 31230683 uc001bsc.2 chr1 31212002 31212079 uc021okj.1 chr1 31342312 31381480 uc001bse.2 chr1 31404352 31538564 uc001bsh.1 chr1 31408535 31408623 uc021okk.1 chr1 31421964 31422052 uc021okl.1 chr1 31441009 31441084 uc001bsl.1 chr1 31652591 31712734 uc010ogd.2 chr1 31732414 31742513 uc009vtt.3 chr1 31732414 31769644 uc001bso.3 chr1 31769841 31837780 uc001bsp.1 chr1 31838099 31845923 uc001bss.1 chr1 31886659 31907527 uc010ogh.2 chr1 31971838 31974167 uc021okn.1 chr1 31984035 31989846 uc001bsy.1 chr1 32042085 32053287 uc001bta.3 chr1 32083300 32092919 uc009vtx.2 chr1 32095462 32110838 uc001bth.2 chr1 32117847 32169768 uc001btk.1 chr1 32192717 32229648 uc001btn.3 chr1 32224260 32224336 uc021okr.1 chr1 32256024 32281580 uc001bts.1 chr1 32372021 32403988 uc001bty.2 chr1 32479294 32509482 uc001bub.4 chr1 32538502 32568467 uc010ogv.2 chr1 32573643 32642168 uc001bug.3 chr1 32645344 32663886 uc001bui.3 chr1 32666201 32670991 uc001bul.1 chr1 32671235 32674288 uc001bum.2 chr1 32674694 32681797 uc001bun.2 chr1 32681797 32687926 uc001buq.4 chr1 32687958 32697205 uc009vuc.3 chr1 32697260 32707311 uc001buv.4 chr1 32712817 32714461 uc001buw.3 chr1 32739711 32751766 uc001buy.3 chr1 32757707 32799224 uc001bvb.1 chr1 32799429 32801840 uc001bvd.4 chr1 32826870 32827844 uc021oku.1 chr1 32827861 32829924 uc001bvf.3 chr1 32830704 32860062 uc010ohg.2 chr1 32930657 32953459 uc001bvl.4 chr1 33004771 33071542 uc001bvn.3 chr1 33087306 33116185 uc001bvp.3 chr1 33116748 33151812 uc001bvr.3 chr1 33145506 33168361 uc001bvt.2 chr1 33207511 33240571 uc001bvu.1 chr1 33240839 33283633 uc001bvy.1 chr1 33283118 33324480 uc001bwc.4 chr1 33327868 33338082 uc001bwg.3 chr1 33352097 33360247 uc001bwh.3 chr1 33360195 33366953 uc001bwi.1 chr1 33402049 33430286 uc010oho.2 chr1 33452675 33498070 uc001bwn.3 chr1 33476825 33502512 uc001bwp.2 chr1 33546713 33585995 uc001bwr.3 chr1 33607471 33608831 uc001bxa.1 chr1 33611002 33647671 uc001bxb.3 chr1 33722173 33766320 uc001bxc.1 chr1 33772366 33786699 uc031plq.1 chr1 33789223 33841194 uc001bxg.1 chr1 33797993 33798093 uc021okw.1 chr1 33802166 33802465 uc021okx.1 chr1 33938231 33961995 uc001bxj.4 chr1 33979608 34631443 uc001bxn.1 chr1 34326075 34330392 uc001bxp.3 chr1 34334556 34351059 uc001bxr.3 chr1 34642552 34684731 uc001bxt.3 chr1 35220647 35224113 uc001bxu.4 chr1 35225341 35229325 uc001bxv.1 chr1 35244163 35244247 uc021ola.1 chr1 35247910 35251967 uc001bxy.3 chr1 35258598 35261348 uc001bya.3 chr1 35315962 35325417 uc001byb.3 chr1 35331036 35370984 uc001byc.3 chr1 35441299 35444307 uc021olf.1 chr1 35447126 35450948 uc001bye.3 chr1 35451766 35497569 uc001byh.3 chr1 35544971 35581455 uc001bym.3 chr1 35641980 35646083 uc001byq.3 chr1 35649200 35658743 uc001bys.3 chr1 35734567 35887545 uc001byt.3 chr1 35833677 35835012 uc031plr.1 chr1 35899090 36023037 uc001byx.3 chr1 36023392 36032380 uc001bza.3 chr1 36038970 36060927 uc010ohy.2 chr1 36065142 36107445 uc001bzf.2 chr1 36179476 36184790 uc001bzh.1 chr1 36197712 36235551 uc001bzi.3 chr1 36273827 36323490 uc001bzj.2 chr1 36348809 36389899 uc001bzl.3 chr1 36391432 36395210 uc001bzm.1 chr1 36396682 36522063 uc001bzp.3 chr1 36549675 36553876 uc001bzr.3 chr1 36554452 36559533 uc001bzt.3 chr1 36560843 36565850 uc001bzv.2 chr1 36602169 36615115 uc031pls.1 chr1 36621802 36646441 uc001bzz.3 chr1 36690016 36770957 uc001cae.4 chr1 36771993 36787379 uc010oia.1 chr1 36787631 36789755 uc001caj.1 chr1 36805224 36851485 uc001cak.1 chr1 36859030 36863493 uc001cao.1 chr1 36883506 36916086 uc001caq.3 chr1 36921361 36930040 uc001cas.2 chr1 36931643 36948915 uc001cax.2 chr1 37261127 37499844 uc001caz.2 chr1 37627163 37627235 uc021oll.1 chr1 37920479 37940044 uc021olm.1 chr1 37940118 37949978 uc001cbb.4 chr1 37955560 37980420 uc001cbe.2 chr1 37966535 37966595 uc031pma.1 chr1 38000049 38019945 uc001cbi.4 chr1 38022519 38032458 uc001cbj.3 chr1 38032412 38061586 uc001cbk.3 chr1 38076950 38100595 uc001cbm.2 chr1 38147241 38156192 uc001cbp.3 chr1 38147242 38149864 uc031pmb.1 chr1 38158072 38175391 uc001cbs.4 chr1 38181645 38230824 uc009vvi.3 chr1 38259773 38267278 uc001cby.2 chr1 38268613 38273865 uc001cca.1 chr1 38273472 38275126 uc001ccd.2 chr1 38275238 38325292 uc001cce.1 chr1 38326368 38412729 uc001ccg.1 chr1 38349908 38349989 uc021oln.1 chr1 38422651 38455761 uc001cci.3 chr1 38462441 38471187 uc001cck.3 chr1 38478383 38490497 uc001ccn.4 chr1 38509522 38512450 uc001ccp.1 chr1 38554902 38555001 uc021olo.1 chr1 38674705 38680439 uc021olp.1 chr1 39303868 39325495 uc001ccq.3 chr1 39328161 39339050 uc001ccs.3 chr1 39340222 39341770 uc021olr.1 chr1 39351478 39407456 uc001ccu.1 chr1 39456915 39471737 uc001ccw.3 chr1 39491966 39500308 uc001ccy.3 chr1 39549838 39952810 uc031pmc.1 chr1 39619835 39619968 uc021olu.1 chr1 39648409 39648505 uc021olv.1 chr1 39875175 39882154 uc009vvt.1 chr1 39957317 39995541 uc001cdi.3 chr1 39970194 39970267 uc021olx.1 chr1 39987951 40025370 uc001cdk.3 chr1 40026484 40042521 uc001cdl.2 chr1 40033045 40033182 uc001cdo.1 chr1 40089102 40105348 uc001cdp.3 chr1 40124792 40137710 uc001cdq.1 chr1 40144644 40157089 uc001cdr.3 chr1 40204516 40229586 uc001cdw.3 chr1 40223902 40254533 uc001cdz.1 chr1 40235196 40237020 uc001ceb.1 chr1 40306705 40349177 uc021olz.1 chr1 40361095 40367687 uc001cer.2 chr1 40420783 40435628 uc001cev.3 chr1 40506254 40538321 uc001cey.4 chr1 40538381 40563142 uc001cfb.2 chr1 40627040 40706593 uc001cfc.4 chr1 40713572 40717365 uc001cfe.2 chr1 40723721 40759856 uc001cfg.4 chr1 40766162 40782981 uc001cfh.1 chr1 40839377 40888998 uc001cfj.3 chr1 40916336 40929390 uc001cfn.2 chr1 40943301 40962015 uc001cfo.3 chr1 40974432 40982214 uc001cfp.3 chr1 40997232 41013841 uc001cft.2 chr1 41086351 41131324 uc001cfu.1 chr1 41154751 41157933 uc010ojl.1 chr1 41157241 41237275 uc009vwd.3 chr1 41220026 41220118 uc001cgf.2 chr1 41222955 41223044 uc001cgg.3 chr1 41249683 41306124 uc001cgh.2 chr1 41326727 41328018 uc001cgj.3 chr1 41347313 41347427 uc021omc.1 chr1 41445006 41478235 uc001cgk.4 chr1 41480261 41509562 uc021omd.1 chr1 41481268 41487427 uc001cgm.2 chr1 41492870 41707815 uc001cgs.3 chr1 41932607 41932699 uc021ome.1 chr1 41944445 41949874 uc009vwh.3 chr1 41944445 41950344 uc001cgx.3 chr1 41972035 42384496 uc001cha.4 chr1 42619091 42621495 uc001chc.1 chr1 42628361 42630395 uc001chd.1 chr1 42642209 42800903 uc001chf.3 chr1 42846467 42889900 uc001chi.2 chr1 42896000 42921938 uc001chj.3 chr1 42922172 42926086 uc001chl.3 chr1 43000559 43120335 uc009vwk.1 chr1 43124047 43142429 uc001chq.3 chr1 43144356 43147330 uc001chr.3 chr1 43148065 43168020 uc001chs.3 chr1 43198763 43205925 uc001cht.1 chr1 43212005 43232755 uc001chx.4 chr1 43232915 43241413 uc001cia.4 chr1 43253660 43263901 uc021omg.1 chr1 43272722 43283059 uc001cib.2 chr1 43291248 43310660 uc001cie.1 chr1 43312243 43318146 uc021omh.1 chr1 43323292 43354460 uc001cij.1 chr1 43391045 43424847 uc001cik.2 chr1 43424719 43449029 uc001cil.3 chr1 43489219 43489308 uc021omj.1 chr1 43585818 43611958 uc009vwn.1 chr1 43613593 43622067 uc009vwo.3 chr1 43629844 43638241 uc010ojx.2 chr1 43638000 43720029 uc021omk.1 chr1 43735664 43739673 uc021oml.1 chr1 43747556 43751250 uc001cit.4 chr1 43766565 43788781 uc001ciu.3 chr1 43803474 43820135 uc001ciw.3 chr1 43824625 43828873 uc001cix.3 chr1 43829067 43833409 uc031pmf.1 chr1 43849578 43855483 uc001cje.2 chr1 43855555 43919918 uc001cjk.3 chr1 43916673 43919660 uc001cjo.3 chr1 43996546 44089343 uc001cjr.3 chr1 44115796 44171189 uc001cjx.3 chr1 44165355 44173012 uc001cjy.4 chr1 44173203 44396837 uc001cjz.4 chr1 44182137 44182227 uc021oms.1 chr1 44398991 44402912 uc001ckt.3 chr1 44412477 44433694 uc001ckx.3 chr1 44435652 44439043 uc001ckz.3 chr1 44440601 44443972 uc001cld.3 chr1 44445607 44456843 uc010okl.2 chr1 44457279 44462198 uc001clj.3 chr1 44462154 44483012 uc001cll.4 chr1 44584521 44600809 uc001clp.3 chr1 44679124 44686351 uc001clq.1 chr1 44686741 44820939 uc001clt.3 chr1 44870959 45117396 uc001clv.1 chr1 45011164 45011224 uc031pmh.1 chr1 45119500 45140099 uc001cmc.3 chr1 45140393 45191263 uc001cmf.2 chr1 45205489 45233438 uc001cmg.4 chr1 45241245 45244412 uc001cmi.3 chr1 45241536 45241610 uc001cmj.1 chr1 45242163 45242261 uc001cmk.1 chr1 45243513 45243584 uc009vxi.3 chr1 45244061 45244130 uc001cml.3 chr1 45249256 45253426 uc001cmm.3 chr1 45266035 45271667 uc001cmn.3 chr1 45271585 45272957 uc001cmp.3 chr1 45274153 45279801 uc010ole.1 chr1 45287937 45308616 uc010olf.2 chr1 45316193 45452394 uc001cmt.3 chr1 45468219 45477027 uc009vxk.3 chr1 45477804 45481341 uc001cna.2 chr1 45482075 45672250 uc001cnd.2 chr1 45769581 45771291 uc010olk.2 chr1 45792544 45794346 uc001cne.3 chr1 45794913 45806142 uc009vxp.3 chr1 45805341 45809650 uc009vxq.3 chr1 45809554 45956840 uc001cns.1 chr1 45959597 45965751 uc001cnw.3 chr1 45965855 45976739 uc009vxv.3 chr1 45976706 45988562 uc021omw.1 chr1 46016454 46035723 uc001coe.3 chr1 46049659 46084578 uc001coi.2 chr1 46085715 46089731 uc010olt.2 chr1 46092975 46152302 uc001coq.3 chr1 46111451 46112357 uc010olu.1 chr1 46153846 46160108 uc001cor.1 chr1 46164406 46216485 uc001cou.3 chr1 46269284 46501796 uc001cov.3 chr1 46505811 46598380 uc001cpb.4 chr1 46640748 46651634 uc001cpd.3 chr1 46654352 46685977 uc001cpg.3 chr1 46669005 46686928 uc010oma.2 chr1 46713366 46744145 uc009vye.2 chr1 46744071 46769038 uc001cpn.3 chr1 46769379 46782447 uc001cpp.3 chr1 46805848 46830824 uc001cpr.2 chr1 46859938 46879520 uc001cpu.2 chr1 46899498 46911374 uc021ona.1 chr1 46972667 46979886 uc001cpx.3 chr1 47004367 47035927 uc021onb.1 chr1 47011315 47015678 uc001cpy.2 chr1 47011315 47016887 uc009vyh.1 chr1 47023078 47069966 uc001cqb.4 chr1 47073386 47080805 uc001cqe.4 chr1 47100710 47134099 uc001cqh.4 chr1 47137496 47139256 uc001cqj.3 chr1 47139707 47157769 uc021ond.1 chr1 47140830 47184736 uc001cqk.4 chr1 47264669 47285021 uc001cqn.4 chr1 47308766 47366147 uc031pmm.1 chr1 47394845 47407156 uc001cqp.4 chr1 47489239 47516423 uc001cqt.3 chr1 47533159 47583992 uc001cqu.1 chr1 47603106 47614526 uc001cqv.1 chr1 47644921 47646011 uc031pmn.1 chr1 47649260 47655771 uc001cqw.3 chr1 47681962 47689770 uc009vyq.2 chr1 47681962 47695443 uc001cqx.2 chr1 47691628 47691655 uc021onf.1 chr1 47715810 47779819 uc001crd.1 chr1 47799468 47844511 uc001cri.3 chr1 47859449 47861215 uc001crj.1 chr1 47881743 47883724 uc001crk.3 chr1 47897806 47900313 uc001crl.3 chr1 47901688 47906363 uc001crm.3 chr1 48226199 48462562 uc021ong.1 chr1 48567386 48648100 uc010omr.1 chr1 48688356 48714316 uc001crn.2 chr1 48761043 48937876 uc001crr.2 chr1 48998526 50489626 uc001cru.2 chr1 49193539 49242547 uc001crx.4 chr1 50574593 50667540 uc001csb.2 chr1 50883222 50889119 uc010onb.2 chr1 50906934 51425936 uc001cse.1 chr1 51048075 51048183 uc021onh.1 chr1 51435641 51440309 uc001csg.3 chr1 51567905 51613754 uc001csh.3 chr1 51701944 51739119 uc001csi.4 chr1 51752929 51810785 uc010onf.2 chr1 51819934 51984995 uc001csq.1 chr1 52004296 52004401 uc021oni.1 chr1 52082545 52254891 uc001csu.3 chr1 52254865 52344609 uc001ctc.4 chr1 52373627 52456436 uc001cth.3 chr1 52461412 52461712 uc021onk.1 chr1 52485803 52521843 uc001cti.4 chr1 52497776 52499472 uc001ctj.1 chr1 52521856 52556388 uc001ctk.3 chr1 52607765 52812358 uc001cto.4 chr1 52816264 52831877 uc001ctq.2 chr1 52838500 52870143 uc001ctu.3 chr1 52870218 52883992 uc001ctv.4 chr1 52870218 52883992 uc001ctw.4 chr1 52888947 53018762 uc001cty.2 chr1 53068042 53074723 uc001cue.3 chr1 53099065 53122737 uc001cuf.3 chr1 53152013 53164038 uc001cui.2 chr1 53192130 53293013 uc001cuj.3 chr1 53308182 53360247 uc001cuk.2 chr1 53361581 53387591 uc001cup.4 chr1 53392900 53517289 uc001cur.2 chr1 53527723 53551174 uc010onr.2 chr1 53552854 53608289 uc001cuy.3 chr1 53580247 53584281 uc001cva.1 chr1 53662100 53679869 uc001cvb.4 chr1 53679771 53686289 uc001cvd.3 chr1 53692563 53704282 uc001cvf.2 chr1 53704281 53708455 uc001cvg.3 chr1 53708040 53793821 uc001cvi.2 chr1 53793904 53802889 uc001cvn.1 chr1 53904042 53905693 uc009vzj.3 chr1 53925071 53933158 uc001cvq.1 chr1 53971905 54199877 uc001cvr.1 chr1 54231133 54304225 uc001cvs.3 chr1 54317391 54355487 uc001cvu.3 chr1 54359860 54376759 uc001cwb.3 chr1 54387233 54411288 uc001cwh.3 chr1 54411998 54433841 uc001cwj.2 chr1 54472970 54483859 uc001cwm.2 chr1 54497348 54519111 uc001cwp.3 chr1 54519273 54565416 uc001cwt.1 chr1 54519751 54519827 uc021ons.1 chr1 54604667 54618679 uc001cwv.2 chr1 54638026 54665746 uc009vzo.3 chr1 54665839 54684056 uc001cxa.4 chr1 54691103 54872068 uc001cxe.4 chr1 55013806 55076005 uc001cxl.2 chr1 55074849 55089200 uc001cxn.3 chr1 55107426 55175939 uc010ooe.1 chr1 55181494 55208328 uc001cxx.4 chr1 55222570 55230226 uc001cxy.3 chr1 55246751 55266941 uc009vzt.1 chr1 55271735 55307937 uc001cyb.4 chr1 55315299 55352921 uc001cyc.1 chr1 55352654 55353883 uc021onu.1 chr1 55423541 55423614 uc021onv.1 chr1 55446464 55457966 uc001cyd.3 chr1 55464616 55474465 uc001cye.3 chr1 55505148 55530526 uc001cyf.2 chr1 55532031 55681039 uc021onw.1 chr1 55681080 55683128 uc021onx.1 chr1 55691313 55691396 uc021ony.1 chr1 55842198 55842525 uc021onz.1 chr1 55950543 55950645 uc021ooa.1 chr1 56046709 56200675 uc001cyi.1 chr1 56960418 57045257 uc001cyj.2 chr1 57110989 57181008 uc001cyk.4 chr1 57184476 57285369 uc001cym.4 chr1 57289353 57292593 uc001cyn.3 chr1 57320442 57383894 uc001cyo.2 chr1 57394882 57431688 uc001cyp.3 chr1 57463578 58716211 uc001cys.1 chr1 58326214 58328786 uc001cyu.1 chr1 58933598 58934677 uc001cyw.1 chr1 58946390 59012446 uc001cyy.3 chr1 59041094 59043166 uc001cyz.4 chr1 59120410 59165747 uc009wab.2 chr1 59246462 59249785 uc001cze.3 chr1 59250822 59365384 uc010oop.1 chr1 59597607 59612479 uc010ooq.2 chr1 59762624 60228402 uc009wac.3 chr1 60198898 60198968 uc021oob.1 chr1 60238466 60254501 uc001czn.3 chr1 60280532 60342050 uc001czo.3 chr1 60358979 60392423 uc001czq.3 chr1 60454823 60539442 uc001czs.2 chr1 61125302 61291256 uc001czt.1 chr1 61405915 61436448 uc001czu.3 chr1 61547533 61928460 uc010oos.2 chr1 62119913 62121800 uc031pmt.1 chr1 62146718 62191095 uc001czz.1 chr1 62208148 62629591 uc001dab.3 chr1 62318170 62318274 uc031pmu.1 chr1 62660473 62678001 uc001dae.4 chr1 62701836 62785083 uc001dah.4 chr1 62901974 62917475 uc001dak.2 chr1 62920396 63154039 uc001daq.4 chr1 63063157 63071976 uc001das.2 chr1 63249776 63330941 uc001dau.3 chr1 63624753 63782901 uc001daw.2 chr1 63704613 63704845 uc021ood.1 chr1 63788729 63790797 uc001dax.2 chr1 63799429 63799491 uc021ooe.1 chr1 63833260 63904233 uc021oof.1 chr1 63906440 63988944 uc001dbb.2 chr1 63989012 64038364 uc001dbf.3 chr1 64014650 64016307 uc001dbg.1 chr1 64088886 64125916 uc010ooz.2 chr1 64239689 64647179 uc001dbj.3 chr1 64262024 64262128 uc021oog.1 chr1 64571005 64636980 uc001dbl.3 chr1 64669489 64710027 uc001dbn.1 chr1 64936475 65158741 uc001dbo.1 chr1 65045529 65045604 uc021ooh.1 chr1 65210777 65298914 uc001dbs.2 chr1 65298905 65432187 uc001dbu.1 chr1 65445259 65468159 uc001dbw.3 chr1 65488650 65488757 uc021ooj.1 chr1 65523437 65523525 uc021ook.1 chr1 65524116 65524191 uc001dbx.3 chr1 65613231 65697828 uc001dby.3 chr1 65775217 65881552 uc001dce.2 chr1 65886130 66103176 uc001dci.3 chr1 65886130 65901690 uc001dcf.3 chr1 66258855 66840262 uc001dco.3 chr1 66560143 66560229 uc021oom.1 chr1 66999824 67210768 uc001dcr.3 chr1 67094122 67094200 uc021oon.1 chr1 67132271 67142710 uc010ope.1 chr1 67218139 67244730 uc001dcv.3 chr1 67263423 67266942 uc001dcw.3 chr1 67278571 67390570 uc001dcx.3 chr1 67390577 67454302 uc001dde.2 chr1 67465014 67520080 uc001ddk.2 chr1 67557858 67600654 uc001ddm.2 chr1 67632168 67725650 uc001ddo.3 chr1 67661822 67661926 uc021ooo.1 chr1 67773046 67862583 uc001ddu.3 chr1 67873492 67896123 uc001ddv.3 chr1 68150859 68154021 uc001ddz.2 chr1 68167148 68299155 uc001dea.2 chr1 68238275 68238336 uc021oop.1 chr1 68297970 68668670 uc001deb.2 chr1 68511644 68516481 uc001ded.3 chr1 68564141 68698284 uc001dee.3 chr1 68649200 68649293 uc021oos.1 chr1 68649301 68649321 uc021oot.1 chr1 68894506 68915642 uc001dei.1 chr1 68939834 68962799 uc001dem.4 chr1 68962358 69004310 uc001den.3 chr1 70225857 70589171 uc001dep.3 chr1 70385004 70386000 uc009wbh.2 chr1 70610484 70671361 uc001der.2 chr1 70671364 70717701 uc001des.3 chr1 70724684 70820417 uc001dex.4 chr1 70820492 70833705 uc001dfa.3 chr1 70876900 70905534 uc001dfd.3 chr1 71172135 71252151 uc001dff.3 chr1 71418114 71513491 uc001dfo.3 chr1 71512188 71532865 uc001dfr.3 chr1 71528973 71546972 uc001dft.3 chr1 71533313 71533399 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uc010pqu.1 chr1 204372491 204380944 uc001hav.4 chr1 204379010 204380945 uc031prt.1 chr1 204391757 204459474 uc001haw.3 chr1 204475654 204475727 uc021phv.1 chr1 204476157 204476230 uc021phw.1 chr1 204485506 204527248 uc001hba.3 chr1 204586302 204654597 uc001hbf.1 chr1 204595902 204598840 uc031pru.1 chr1 204676447 204676558 uc021phz.1 chr1 204797781 204991950 uc001hbj.3 chr1 204981852 204991950 uc001hbo.2 chr1 204989426 204991950 uc001hbp.1 chr1 205012339 205047171 uc001hbr.3 chr1 205052256 205053588 uc001hbt.3 chr1 205055269 205091150 uc001hbu.2 chr1 205111630 205180727 uc001hbw.3 chr1 205197037 205242471 uc021pia.1 chr1 205271190 205290883 uc001hce.3 chr1 205305192 205326218 uc031prx.1 chr1 205342379 205356568 uc001hch.1 chr1 205350505 205391214 uc001hcj.2 chr1 205417429 205417526 uc010prh.1 chr1 205425185 205438152 uc001hco.2 chr1 205443270 205443343 uc021pib.1 chr1 205473683 205495965 uc010pri.2 chr1 205473683 205501921 uc001hcr.3 chr1 205523400 205525763 uc001hcu.2 chr1 205538111 205572046 uc001hcv.4 chr1 205564168 205564275 uc021pic.1 chr1 205626980 205649630 uc001hda.1 chr1 205681946 205719372 uc001hdb.3 chr1 205737113 205744610 uc001hde.4 chr1 205758220 205782161 uc001hdh.1 chr1 205765004 205765744 uc001hdi.1 chr1 205797149 205819276 uc001hdj.3 chr1 205831206 205865215 uc001hdk.1 chr1 205882176 205912588 uc001hdp.3 chr1 206138910 206155074 uc001hdr.4 chr1 206224282 206231482 uc001hds.2 chr1 206238881 206288236 uc001hdt.2 chr1 206317458 206332104 uc001hdu.3 chr1 206516199 206637783 uc001hdy.3 chr1 206643585 206670223 uc001hdz.2 chr1 206680878 206762616 uc001hed.3 chr1 206764973 206785904 uc001heh.2 chr1 206808880 206822542 uc001hej.3 chr1 206858364 206907630 uc001hem.2 chr1 206940947 206945839 uc001hen.1 chr1 206972214 207016326 uc001heo.3 chr1 207039153 207042568 uc001her.3 chr1 207070787 207077484 uc001heu.2 chr1 207076630 207095378 uc001hey.3 chr1 207101866 207119811 uc001hez.3 chr1 207131311 207143970 uc001hfa.4 chr1 207178153 207178230 uc021pih.1 chr1 207191865 207206101 uc001hfd.2 chr1 207217193 207224422 uc001hfe.1 chr1 207226619 207251162 uc001hfg.3 chr1 207262211 207273337 uc001hfj.3 chr1 207277510 207277617 uc001hfn.1 chr1 207277606 207318317 uc001hfo.3 chr1 207494816 207534311 uc001hfr.4 chr1 207627644 207663240 uc001hfv.3 chr1 207669472 207815110 uc001hfx.3 chr1 207818457 207897036 uc001hga.4 chr1 207925382 207968861 uc001hgj.3 chr1 207975196 207975868 uc021pik.2 chr1 207991723 207995941 uc010psi.2 chr1 208039465 208042417 uc001hgu.2 chr1 208059882 208084683 uc001hgw.1 chr1 208195587 208417665 uc001hgz.3 chr1 209602167 209605892 uc009xcn.3 chr1 209757044 209787284 uc001hhd.3 chr1 209788217 209825820 uc001hhh.3 chr1 209796788 209796855 uc021pil.1 chr1 209848669 209849735 uc001hhi.4 chr1 209878135 209908295 uc001hhk.3 chr1 209929393 209955668 uc001hho.3 chr1 209955661 209957890 uc001hhp.1 chr1 209958967 209979520 uc001hhq.2 chr1 210001311 210030910 uc001hhr.2 chr1 210111518 210337633 uc001hhs.5 chr1 210404803 210407466 uc001hhx.4 chr1 210406194 210416440 uc001hhy.3 chr1 210501595 210849638 uc009xcx.3 chr1 210851656 211307457 uc001hib.2 chr1 211432707 211489725 uc010psw.2 chr1 211500147 211548286 uc001hii.3 chr1 211556096 211605877 uc001hil.4 chr1 211649863 211666259 uc001hin.2 chr1 211748380 211752099 uc001hio.1 chr1 211836113 211848972 uc001hir.2 chr1 211916798 212004114 uc001hiv.3 chr1 212113740 212209002 uc001hiw.2 chr1 212208918 212278187 uc009xdc.3 chr1 212250954 212251027 uc021pis.1 chr1 212317864 212318301 uc010ptc.1 chr1 212458878 212535205 uc001hjb.3 chr1 212526159 212526292 uc009xdf.1 chr1 212537815 212588267 uc010pte.2 chr1 212606228 212619721 uc001hjd.3 chr1 212781969 212794119 uc021pit.1 chr1 212797625 212800113 uc010pth.1 chr1 212797788 212800120 uc001hjk.3 chr1 212859758 212873327 uc001hjl.2 chr1 212899494 212965139 uc001hjn.3 chr1 212965169 212990167 uc001hjo.2 chr1 213003484 213020991 uc001hjq.3 chr1 213029945 213031480 uc001hjs.5 chr1 213031596 213072705 uc001hjt.3 chr1 213123886 213164927 uc001hjw.3 chr1 213165523 213189168 uc001hjz.3 chr1 213224587 213446808 uc010ptr.2 chr1 213992977 214139607 uc031psa.1 chr1 214098091 214099996 uc031psb.1 chr1 214161277 214214847 uc001hkg.2 chr1 214454564 214510477 uc021pix.1 chr1 214522038 214725024 uc001hkk.2 chr1 214655881 214656819 uc010ptz.1 chr1 214776531 214837914 uc001hkm.3 chr1 215256559 215410436 uc001hkr.4 chr1 215740734 215795149 uc001hks.3 chr1 215796235 216596738 uc001hku.1 chr1 216676587 216896814 uc001hkw.2 chr1 216825011 216825068 uc021pjb.1 chr1 217603833 217804409 uc001hlf.1 chr1 217804694 218040484 uc001hlh.1 chr1 218066241 218094146 uc031psc.1 chr1 218458628 218511325 uc001hlj.3 chr1 218517537 218519020 uc031psd.1 chr1 218518675 218617961 uc001hln.3 chr1 219254316 219347130 uc001hlp.3 chr1 219347191 219386207 uc001hlq.4 chr1 220046618 220292777 uc021pjd.1 chr1 220087605 220101993 uc001hlw.3 chr1 220141941 220220000 uc001hly.1 chr1 220230823 220263191 uc001hma.3 chr1 220267454 220321383 uc001hmc.3 chr1 220291194 220291304 uc010pui.2 chr1 220291498 220291583 uc010puj.2 chr1 220291547 220291569 uc021pje.1 chr1 220321609 220445843 uc010puk.1 chr1 220373883 220374018 uc010pul.2 chr1 220439520 220441057 uc001hmj.2 chr1 220701567 220837799 uc001hmn.4 chr1 220863627 220872499 uc001hmp.1 chr1 220921675 220957596 uc001hmq.3 chr1 220960038 220987741 uc001hms.3 chr1 220999117 220999235 uc021pjg.1 chr1 221002596 221005770 uc001hmu.3 chr1 221052742 221058400 uc001hmv.4 chr1 221503269 221509638 uc001hmw.1 chr1 221874763 221915516 uc001hmy.2 chr1 222638346 222638419 uc021pjh.1 chr1 222646009 222646043 uc010puo.1 chr1 222646722 222646767 uc001hna.1 chr1 222646839 222646870 uc001hnb.3 chr1 222647751 222647786 uc010pup.1 chr1 222648391 222648423 uc021pji.1 chr1 222648536 222648596 uc001hnd.3 chr1 222648680 222648717 uc021pjj.1 chr1 222649002 222649064 uc010puq.2 chr1 222649161 222649251 uc021pjk.1 chr1 222650319 222650381 uc001hnf.3 chr1 222650411 222650443 uc001hng.2 chr1 222695601 222721444 uc001hnh.1 chr1 222731243 222763275 uc009xdz.2 chr1 222791443 222841351 uc001hnl.3 chr1 222841354 222885864 uc001hnn.3 chr1 222885905 222906106 uc001hnq.1 chr1 222900275 222946483 uc001hnr.1 chr1 222906202 222908533 uc001hns.1 chr1 222910557 222924002 uc001hnt.3 chr1 222988430 223179337 uc001hnu.2 chr1 223282747 223316624 uc001hnw.2 chr1 223394160 223537544 uc001hny.4 chr1 223566714 223568812 uc001hoa.2 chr1 223714971 223853436 uc009xee.2 chr1 223900118 223963720 uc001hob.4 chr1 223967594 224033674 uc010pvb.2 chr1 224051997 224052306 uc021pjm.1 chr1 224139721 224139941 uc001hof.1 chr1 224189608 224197818 uc001hog.1 chr1 224294983 224295243 uc021pjn.1 chr1 224301788 224349749 uc001hoh.3 chr1 224370909 224381142 uc001hoj.3 chr1 224415035 224517891 uc001hok.3 chr1 224444705 224444843 uc021pjo.1 chr1 224544512 224566223 uc001hom.2 chr1 224572844 224622001 uc001hop.4 chr1 224585928 224586013 uc021pjq.1 chr1 224586541 224586601 uc021pjr.1 chr1 224804178 224928249 uc001hos.1 chr1 225117355 225586996 uc001how.2 chr1 225589203 225615815 uc001hoy.3 chr1 225674533 225840845 uc001hpc.1 chr1 225888305 225939945 uc001hpe.1 chr1 225965514 225978168 uc001hpg.3 chr1 225997796 226033262 uc001hpk.3 chr1 226033232 226070420 uc001hpm.2 chr1 226073981 226076846 uc001hpo.3 chr1 226107576 226112040 uc001hpq.4 chr1 226124297 226129083 uc001hpt.2 chr1 226170402 226187066 uc001hpu.4 chr1 226250407 226259703 uc001hpw.3 chr1 226271655 226277994 uc001hpx.3 chr1 226332379 226374423 uc001hpy.3 chr1 226374532 226385086 uc031psl.1 chr1 226411382 226413513 uc010pvm.2 chr1 226418849 226497204 uc001hqa.3 chr1 226548391 226595801 uc001hqd.4 chr1 226723318 226730469 uc001hqe.2 chr1 226736500 226796915 uc021pjw.1 chr1 226819390 226926876 uc010pvo.2 chr1 227058272 227083804 uc009xeo.1 chr1 227127937 227175246 uc001hqn.1 chr1 227177565 227505826 uc001hqr.3 chr1 227581291 227618723 uc001hqv.3 chr1 227751219 227850164 uc021pjy.1 chr1 227884732 227885408 uc021pjz.1 chr1 227918889 227923112 uc001hrb.3 chr1 227922696 227968932 uc001hrf.2 chr1 228003417 228034171 uc001hrh.3 chr1 228109164 228135676 uc001hri.2 chr1 228129290 228129384 uc031psm.1 chr1 228155293 228155325 uc001hrj.3 chr1 228155355 228155417 uc001hrk.3 chr1 228156485 228156575 uc021pka.1 chr1 228156672 228156704 uc021pkb.1 chr1 228157022 228157062 uc010pvt.1 chr1 228157172 228157203 uc010pvu.2 chr1 228160064 228160098 uc001hrn.2 chr1 228160168 228160213 uc021pkc.1 chr1 228194722 228248972 uc001hrq.2 chr1 228270360 228286913 uc001hrr.3 chr1 228284963 228285042 uc021pkd.1 chr1 228288427 228291022 uc001hrx.3 chr1 228294379 228297013 uc001hrz.4 chr1 228327928 228336655 uc021pkf.1 chr1 228337414 228347527 uc001hsk.3 chr1 228353428 228369958 uc001hsl.4 chr1 228391206 228401365 uc031psn.1 chr1 228395830 228566575 uc001hsq.2 chr1 228581376 228594517 uc001hss.3 chr1 228595635 228604583 uc001hsv.3 chr1 228612545 228613026 uc001hsx.1 chr1 228645064 228645560 uc001hsy.3 chr1 228645807 228646259 uc001hsz.3 chr1 228649774 228649853 uc021pkh.1 chr1 228651845 228651891 uc021pki.1 chr1 228673888 228674821 uc001hta.3 chr1 228675067 228683889 uc001htb.3 chr1 228780393 228788159 uc001htd.2 chr1 228870823 228882416 uc001htf.3 chr1 229050379 229052954 uc001htg.2 chr1 229406808 229441640 uc001hth.4 chr1 229440128 229441250 uc001htk.4 chr1 229456751 229478688 uc001htl.4 chr1 229566992 229569843 uc001htm.3 chr1 229577043 229644088 uc001htn.3 chr1 229589676 229591617 uc001hto.1 chr1 229652328 229694442 uc001htp.4 chr1 229728865 229761794 uc001htq.3 chr1 229761980 229795946 uc001hts.1 chr1 230202955 230417875 uc010pwa.1 chr1 230457391 230561674 uc031psq.1 chr1 230778201 230829731 uc001htw.3 chr1 230838271 230850336 uc001hty.4 chr1 230883129 230937749 uc001htz.1 chr1 230972864 231004302 uc001hub.3 chr1 231010591 231014761 uc001hue.1 chr1 231041986 231114618 uc001huf.4 chr1 231114822 231136479 uc001huh.3 chr1 231154703 231175995 uc001hui.2 chr1 231298673 231357314 uc009xfn.1 chr1 231319844 231323373 uc031pss.1 chr1 231359508 231376924 uc001hul.3 chr1 231376918 231413719 uc001hup.4 chr1 231468481 231473578 uc001huq.3 chr1 231473681 231490769 uc001hur.4 chr1 231499496 231560790 uc001huv.2 chr1 231611509 231612271 uc021pkl.1 chr1 231664398 231702269 uc001huw.3 chr1 231727037 231747836 uc021pkm.2 chr1 231762560 232177019 uc010pxh.2 chr1 231950371 231954263 uc001hvd.3 chr1 232533711 232651243 uc001hvg.3 chr1 232940637 232946092 uc001hvh.2 chr1 233086369 233114219 uc001hvj.1 chr1 233119881 233431459 uc001hvl.2 chr1 233463513 233520894 uc001hvt.4 chr1 233584371 233584527 uc021pko.1 chr1 233749749 233808258 uc010pxo.1 chr1 233759897 233759965 uc021pkp.1 chr1 234040678 234460262 uc001hvy.1 chr1 234348351 234350834 uc001hvz.1 chr1 234442212 234442285 uc021pkq.1 chr1 234509428 234519795 uc001hwc.3 chr1 234527058 234614849 uc001hwd.3 chr1 234663636 234667525 uc001hwe.3 chr1 234740014 234745271 uc001hwg.3 chr1 234765056 234770526 uc021pkr.1 chr1 234782034 234796670 uc001hwh.3 chr1 234859788 234867390 uc001hwj.2 chr1 235093089 235099746 uc001hwk.4 chr1 235272657 235292256 uc001hwl.3 chr1 235291117 235291252 uc001hwm.1 chr1 235294497 235324772 uc001hwn.3 chr1 235330209 235491532 uc001hwq.3 chr1 235353348 235353431 uc021pkt.1 chr1 235491752 235507844 uc001hwv.3 chr1 235530727 235612280 uc001hxa.1 chr1 235610504 235667781 uc001hxc.3 chr1 235710984 235814054 uc001hxh.4 chr1 235712454 235714587 uc021pku.1 chr1 235824330 236030227 uc001hxj.3 chr1 236016299 236016360 uc021pkv.1 chr1 236139131 236228481 uc001hxo.3 chr1 236227659 236229779 uc001hxp.1 chr1 236305831 236372209 uc001hxq.3 chr1 236378421 236445339 uc001hxt.3 chr1 236557679 236648008 uc001hxu.1 chr1 236686368 236687808 uc001hxx.3 chr1 236686738 236716279 uc001hxy.2 chr1 236712304 236767841 uc001hyd.2 chr1 236849769 236927558 uc001hyf.2 chr1 236958580 237067281 uc001hyi.4 chr1 236973802 236992568 uc009xgj.1 chr1 237167402 237167718 uc001hyk.2 chr1 237205701 237997288 uc001hyl.1 chr1 237284106 237284409 uc021pkw.1 chr1 238025474 238091619 uc010pyc.2 chr1 238041163 238054222 uc001hym.3 chr1 238105861 238105933 uc021pla.1 chr1 238106955 238107030 uc021plb.1 chr1 238643683 238649317 uc001hyn.3 chr1 239792372 240072717 uc001hyp.3 chr1 240170823 240176560 uc021pld.1 chr1 240255184 240638489 uc010pyd.2 chr1 240317950 240318072 uc021ple.1 chr1 240652872 240775462 uc001hys.3 chr1 240938816 241520478 uc001hyv.2 chr1 241295571 241295646 uc021plg.1 chr1 241660856 241683085 uc001hyx.3 chr1 241695433 241758949 uc009xgp.3 chr1 241756451 241803701 uc001hza.3 chr1 241792166 241799232 uc001hzd.3 chr1 241815579 241965434 uc001hzg.2 chr1 242011492 242053241 uc001hzh.3 chr1 242158791 242162385 uc001hzk.2 chr1 242251688 242687998 uc001hzn.2 chr1 243218732 243218763 uc021plm.1 chr1 243219238 243219328 uc021pln.1 chr1 243219615 243265046 uc001hzq.2 chr1 243287729 243418708 uc021plo.1 chr1 243351942 243352032 uc021plr.1 chr1 243419306 243663393 uc001hzw.3 chr1 243509477 243509557 uc021plt.1 chr1 243663020 244006584 uc001iab.2 chr1 243729624 243729741 uc021plv.1 chr1 244080703 244210619 uc001iac.3 chr1 244212240 244220780 uc031psv.1 chr1 244515936 244552388 uc001iah.4 chr1 244571793 244615436 uc001iaj.3 chr1 244624672 244803662 uc001iam.3 chr1 244816351 244872334 uc001iao.3 chr1 244998638 245008359 uc001iar.3 chr1 245003939 245010243 uc001iav.3 chr1 245013601 245027827 uc001iaz.1 chr1 245133170 245251148 uc001ibc.2 chr1 245318286 245866428 uc001ibf.1 chr1 245533635 245533719 uc021plw.1 chr1 245912641 246670644 uc001ibl.3 chr1 246679340 246687589 uc021plx.1 chr1 246703862 246729565 uc001ibn.3 chr1 246729638 246831884 uc001ibp.3 chr1 246887377 246931440 uc001ibr.3 chr1 246939314 246955685 uc001ibs.1 chr1 246970416 246970487 uc021ply.1 chr1 247002401 247094726 uc001ibv.2 chr1 247148624 247171395 uc009xgu.3 chr1 247197939 247242115 uc001icd.2 chr1 247263263 247267674 uc001ice.2 chr1 247273461 247275719 uc001icg.1 chr1 247319202 247335318 uc001icj.1 chr1 247337903 247338870 uc001icl.1 chr1 247365268 247365362 uc021pma.1 chr1 247419373 247420447 uc010pyu.2 chr1 247463621 247495045 uc001ico.3 chr1 247579457 247612406 uc001icr.3 chr1 247614330 247615284 uc010pyx.2 chr1 247654369 247655711 uc001icz.2 chr1 247681594 247683942 uc001idc.3 chr1 247693433 247697141 uc009xgy.3 chr1 247712346 247739848 uc001idf.3 chr1 247751661 247752615 uc010pyy.2 chr1 247768887 247769817 uc010pyz.2 chr1 247835419 247836343 uc001idi.1 chr1 247875130 247876057 uc001idj.1 chr1 247920763 247921708 uc010pza.2 chr1 247978101 247979031 uc001idm.1 chr1 248004229 248005198 uc001idn.1 chr1 248020500 248043438 uc001ido.3 chr1 248058888 248059833 uc010pzb.2 chr1 248084319 248085258 uc010pzc.2 chr1 248100492 248264224 uc001ids.3 chr1 248112159 248113098 uc001idt.1 chr1 248128633 248129641 uc010pzd.2 chr1 248153568 248154493 uc001idv.1 chr1 248185249 248186188 uc031psy.1 chr1 248201473 248202607 uc001idw.3 chr1 248223983 248224922 uc001idx.1 chr1 248285437 248286082 uc001idy.1 chr1 248308449 248309388 uc010pze.2 chr1 248343287 248344331 uc010pzf.2 chr1 248366369 248367308 uc010pzg.2 chr1 248402230 248403166 uc010pzh.2 chr1 248436153 248437116 uc010pzi.2 chr1 248457917 248458880 uc010pzj.2 chr1 248486931 248487870 uc010pzk.2 chr1 248512076 248513015 uc010pzl.2 chr1 248524882 248525929 uc001ieh.1 chr1 248550909 248551836 uc001iei.1 chr1 248569295 248570405 uc010pzm.2 chr1 248616098 248617073 uc001iek.1 chr1 248636651 248637608 uc001iel.1 chr1 248651889 248652837 uc001iem.1 chr1 248684947 248685898 uc001ien.1 chr1 248721844 248722792 uc001ieo.2 chr1 248737101 248738058 uc001iep.1 chr1 248756130 248757069 uc010pzn.2 chr1 248789478 248790429 uc001ier.1 chr1 248801587 248802559 uc001ies.1 chr1 248813231 248814185 uc010pzo.2 chr1 248844669 248845605 uc001ieu.1 chr1 248881947 248885507 uc031psz.1 chr1 248902716 248903151 uc009xhb.3 chr1 249104650 249120154 uc001iew.1 chr1 249120575 249120642 uc021pmd.1 chr1 249132529 249143714 uc001iex.3 chr1 249144202 249153315 uc001ifc.2 chr1 249168053 249168159 uc021pmf.1 chr1 249168446 249168518 uc021pmg.1 chr1 249200441 249213345 uc001ifh.3 ================================================ FILE: intro_awk_spring2021/data/.ipynb_checkpoints/example-checkpoint.fastq ================================================ @ERR117184_2.14044282 CTGGTGCTCAGTCATTTTGCTAGATTGTAGCTCACCATTGCCTCTCTGCCTGCATTGTGCACTCCAATCCCTCAGCAGCTACAAAAACACTTTGTCAACTCCAGATCGG + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117185_2.23793537 TTGTTCAATTTTTTTAGTAGTTTTAGAGTAATTAATGAGCTTTGAGGTCACTTAACAATAAAAGCATATTTTAAAGTAAAGGTTGCTGATCAACAGTCTAATTTTCATA + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117166_2.4649205 ACTTAACAATAAAAGCATATTTTAAAGTAAAGGTT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117185_2.949669 ATGCTTTTATTGTTAAGTGACCTCAAAGCTCATTAATTACTCTAAAACTACTAAAAAAATTGAACAAAAAAGTATTTCCAGTAATCAAGATTTTCTTCATGCCCTAGAT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117163_2.2292219 AAATCGTAAGTGAAGCAACAGAATTCAGAACTAAA + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117165_2.36526615 AACGCTAAGGTTATATTAAAATGTTTCTTATGTTG + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117174_2.23007391 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117175_2.11345437 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117176_2.30285510 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117177_2.33680511 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII ================================================ FILE: intro_awk_spring2021/data/.ipynb_checkpoints/hg38-checkpoint.genome ================================================ chrom size 1 248956422 2 242193529 3 198295559 4 190214555 5 181538259 6 170805979 7 159345973 X 156040895 8 145138636 9 138394717 11 135086622 10 133797422 12 133275309 13 114364328 14 107043718 15 101991189 16 90338345 17 83257441 18 80373285 20 64444167 19 58617616 Y 57227415 22 50818468 21 46709983 15_KI270905v1_alt 5161414 6_GL000256v2_alt 4929269 6_GL000254v2_alt 4827813 6_GL000251v2_alt 4795265 6_GL000253v2_alt 4677643 6_GL000250v2_alt 4672374 6_GL000255v2_alt 4606388 6_GL000252v2_alt 4604811 17_KI270857v1_alt 2877074 16_KI270853v1_alt 2659700 16_KI270728v1_random 1872759 17_GL000258v2_alt 1821992 5_GL339449v2_alt 1612928 14_KI270847v1_alt 1511111 17_KI270908v1_alt 1423190 14_KI270846v1_alt 1351393 5_KI270897v1_alt 1144418 7_KI270803v1_alt 1111570 19_GL949749v2_alt 1091841 19_KI270938v1_alt 1066800 19_GL949750v2_alt 1066390 19_GL949748v2_alt 1064304 19_GL949751v2_alt 1002683 19_GL949746v1_alt 987716 19_GL949752v1_alt 987100 8_KI270821v1_alt 985506 1_KI270763v1_alt 911658 6_KI270801v1_alt 870480 19_GL949753v2_alt 796479 19_GL949747v2_alt 729520 8_KI270822v1_alt 624492 4_GL000257v2_alt 586476 12_KI270904v1_alt 572349 4_KI270925v1_alt 555799 15_KI270852v1_alt 478999 15_KI270727v1_random 448248 9_KI270823v1_alt 439082 15_KI270850v1_alt 430880 1_KI270759v1_alt 425601 12_GL877876v1_alt 408271 Un_KI270442v1 392061 17_KI270862v1_alt 391357 15_GL383555v2_alt 388773 19_GL383573v1_alt 385657 4_KI270896v1_alt 378547 4_GL383528v1_alt 376187 17_GL383563v3_alt 375691 8_KI270810v1_alt 374415 1_GL383520v2_alt 366580 1_KI270762v1_alt 354444 15_KI270848v1_alt 327382 17_KI270909v1_alt 325800 14_KI270844v1_alt 322166 8_KI270900v1_alt 318687 10_GL383546v1_alt 309802 13_KI270838v1_alt 306913 8_KI270816v1_alt 305841 22_KI270879v1_alt 304135 8_KI270813v1_alt 300230 11_KI270831v1_alt 296895 15_GL383554v1_alt 296527 8_KI270811v1_alt 292436 18_GL383567v1_alt 289831 X_KI270880v1_alt 284869 8_KI270812v1_alt 282736 19_KI270921v1_alt 282224 17_KI270729v1_random 280839 17_JH159146v1_alt 278131 X_KI270913v1_alt 274009 6_KI270798v1_alt 271782 7_KI270808v1_alt 271455 22_KI270876v1_alt 263666 15_KI270851v1_alt 263054 22_KI270875v1_alt 259914 1_KI270766v1_alt 256271 19_KI270882v1_alt 248807 3_KI270778v1_alt 248252 15_KI270849v1_alt 244917 4_KI270786v1_alt 244096 12_KI270835v1_alt 238139 17_KI270858v1_alt 235827 19_KI270867v1_alt 233762 16_KI270855v1_alt 232857 8_KI270926v1_alt 229282 5_GL949742v1_alt 226852 3_KI270780v1_alt 224108 17_GL383565v1_alt 223995 2_KI270774v1_alt 223625 4_KI270790v1_alt 220246 11_KI270927v1_alt 218612 19_KI270932v1_alt 215732 11_KI270903v1_alt 214625 2_KI270894v1_alt 214158 14_GL000225v1_random 211173 Un_KI270743v1 210658 11_KI270832v1_alt 210133 7_KI270805v1_alt 209988 4_GL000008v2_random 209709 7_KI270809v1_alt 209586 19_KI270887v1_alt 209512 4_KI270789v1_alt 205944 3_KI270779v1_alt 205312 19_KI270914v1_alt 205194 19_KI270886v1_alt 204239 11_KI270829v1_alt 204059 14_GL000009v2_random 201709 21_GL383579v2_alt 201197 11_JH159136v1_alt 200998 19_KI270930v1_alt 200773 Un_KI270747v1 198735 18_GL383571v1_alt 198278 19_KI270920v1_alt 198005 6_KI270797v1_alt 197536 3_KI270935v1_alt 197351 17_KI270861v1_alt 196688 15_KI270906v1_alt 196384 5_KI270791v1_alt 195710 14_KI270722v1_random 194050 16_GL383556v1_alt 192462 13_KI270840v1_alt 191684 14_GL000194v1_random 191469 11_JH159137v1_alt 191409 19_KI270917v1_alt 190932 7_KI270899v1_alt 190869 19_KI270923v1_alt 189352 10_KI270825v1_alt 188315 19_GL383576v1_alt 188024 19_KI270922v1_alt 187935 Un_KI270742v1 186739 22_KI270878v1_alt 186262 19_KI270929v1_alt 186203 11_KI270826v1_alt 186169 6_KB021644v2_alt 185823 17_GL000205v2_random 185591 1_KI270765v1_alt 185285 19_KI270916v1_alt 184516 19_KI270890v1_alt 184499 3_KI270784v1_alt 184404 12_GL383551v1_alt 184319 20_KI270870v1_alt 183433 Un_GL000195v1 182896 1_GL383518v1_alt 182439 22_KI270736v1_random 181920 10_KI270824v1_alt 181496 14_KI270845v1_alt 180703 3_GL383526v1_alt 180671 13_KI270839v1_alt 180306 22_KI270733v1_random 179772 Un_GL000224v1 179693 10_GL383545v1_alt 179254 Un_GL000219v1 179198 5_KI270792v1_alt 179043 17_KI270860v1_alt 178921 19_GL000209v2_alt 177381 11_KI270830v1_alt 177092 9_KI270719v1_random 176845 Un_GL000216v2 176608 22_KI270928v1_alt 176103 1_KI270712v1_random 176043 6_KI270800v1_alt 175808 1_KI270706v1_random 175055 2_KI270776v1_alt 174166 18_KI270912v1_alt 174061 3_KI270777v1_alt 173649 5_GL383531v1_alt 173459 3_JH636055v2_alt 173151 14_KI270725v1_random 172810 5_KI270796v1_alt 172708 9_GL383541v1_alt 171286 19_KI270885v1_alt 171027 19_KI270919v1_alt 170701 19_KI270889v1_alt 170698 19_KI270891v1_alt 170680 19_KI270915v1_alt 170665 19_KI270933v1_alt 170537 19_KI270883v1_alt 170399 19_GL383575v2_alt 170222 19_KI270931v1_alt 170148 12_GL383550v2_alt 169178 13_KI270841v1_alt 169134 Un_KI270744v1 168472 18_KI270863v1_alt 167999 18_GL383569v1_alt 167950 12_GL877875v1_alt 167313 21_KI270874v1_alt 166743 3_KI270924v1_alt 166540 1_KI270761v1_alt 165834 3_KI270937v1_alt 165607 22_KI270734v1_random 165050 18_GL383570v1_alt 164789 5_KI270794v1_alt 164558 4_GL383527v1_alt 164536 Un_GL000213v1 164239 3_KI270936v1_alt 164170 3_KI270934v1_alt 163458 9_GL383539v1_alt 162988 3_KI270895v1_alt 162896 22_GL383582v2_alt 162811 3_KI270782v1_alt 162429 1_KI270892v1_alt 162212 Un_GL000220v1 161802 2_KI270767v1_alt 161578 2_KI270715v1_random 161471 2_KI270893v1_alt 161218 Un_GL000218v1 161147 18_GL383572v1_alt 159547 8_KI270817v1_alt 158983 4_KI270788v1_alt 158965 Un_KI270749v1 158759 7_KI270806v1_alt 158166 7_KI270804v1_alt 157952 18_KI270911v1_alt 157710 Un_KI270741v1 157432 17_KI270910v1_alt 157099 19_KI270884v1_alt 157053 19_GL383574v1_alt 155864 19_KI270888v1_alt 155532 3_GL000221v1_random 155397 11_GL383547v1_alt 154407 2_KI270716v1_random 153799 12_GL383553v2_alt 152874 6_KI270799v1_alt 152148 22_KI270731v1_random 150754 Un_KI270751v1 150742 Un_KI270750v1 148850 8_KI270818v1_alt 145606 X_KI270881v1_alt 144206 21_KI270873v1_alt 143900 2_GL383521v1_alt 143390 8_KI270814v1_alt 141812 12_GL383552v1_alt 138655 Un_KI270519v1 138126 2_KI270775v1_alt 138019 17_KI270907v1_alt 137721 Un_GL000214v1 137718 8_KI270901v1_alt 136959 2_KI270770v1_alt 136240 16_KI270854v1_alt 134193 8_KI270819v1_alt 133535 17_GL383564v2_alt 133151 2_KI270772v1_alt 133041 8_KI270815v1_alt 132244 5_KI270795v1_alt 131892 5_KI270898v1_alt 130957 20_GL383577v2_alt 128386 1_KI270708v1_random 127682 7_KI270807v1_alt 126434 5_KI270793v1_alt 126136 6_GL383533v1_alt 124736 2_GL383522v1_alt 123821 19_KI270918v1_alt 123111 12_GL383549v1_alt 120804 2_KI270769v1_alt 120616 4_KI270785v1_alt 119912 12_KI270834v1_alt 119498 7_GL383534v2_alt 119183 20_KI270869v1_alt 118774 21_GL383581v2_alt 116689 3_KI270781v1_alt 113034 17_KI270730v1_random 112551 Un_KI270438v1 112505 4_KI270787v1_alt 111943 18_KI270864v1_alt 111737 2_KI270771v1_alt 110395 1_GL383519v1_alt 110268 2_KI270768v1_alt 110099 1_KI270760v1_alt 109528 3_KI270783v1_alt 109187 17_KI270859v1_alt 108763 11_KI270902v1_alt 106711 18_GL383568v1_alt 104552 22_KI270737v1_random 103838 13_KI270843v1_alt 103832 22_KI270877v1_alt 101331 5_GL383530v1_alt 101241 11_KI270721v1_random 100316 22_KI270738v1_random 99375 22_GL383583v2_alt 96924 2_GL582966v2_alt 96131 Un_KI270748v1 93321 Un_KI270435v1 92983 5_GL000208v1_random 92689 Un_KI270538v1 91309 17_GL383566v1_alt 90219 16_GL383557v1_alt 89672 17_JH159148v1_alt 88070 5_GL383532v1_alt 82728 21_KI270872v1_alt 82692 Un_KI270756v1 79590 6_KI270758v1_alt 76752 12_KI270833v1_alt 76061 6_KI270802v1_alt 75005 21_GL383580v2_alt 74653 22_KB663609v1_alt 74013 22_KI270739v1_random 73985 9_GL383540v1_alt 71551 Un_KI270757v1 71251 2_KI270773v1_alt 70887 17_JH159147v1_alt 70345 11_KI270827v1_alt 67707 1_KI270709v1_random 66860 Un_KI270746v1 66486 16_KI270856v1_alt 63982 21_GL383578v2_alt 63917 Un_KI270753v1 62944 19_KI270868v1_alt 61734 9_GL383542v1_alt 60032 20_KI270871v1_alt 58661 12_KI270836v1_alt 56134 19_KI270865v1_alt 52969 1_KI270764v1_alt 50258 Un_KI270589v1 44474 14_KI270726v1_random 43739 19_KI270866v1_alt 43156 22_KI270735v1_random 42811 1_KI270711v1_random 42210 Un_KI270745v1 41891 1_KI270714v1_random 41717 22_KI270732v1_random 41543 1_KI270713v1_random 40745 Un_KI270754v1 40191 1_KI270710v1_random 40176 12_KI270837v1_alt 40090 9_KI270717v1_random 40062 14_KI270724v1_random 39555 9_KI270720v1_random 39050 14_KI270723v1_random 38115 9_KI270718v1_random 38054 Un_KI270317v1 37690 13_KI270842v1_alt 37287 Y_KI270740v1_random 37240 Un_KI270755v1 36723 8_KI270820v1_alt 36640 1_KI270707v1_random 32032 Un_KI270579v1 31033 Un_KI270752v1 27745 Un_KI270512v1 22689 Un_KI270322v1 21476 M 16569 Un_GL000226v1 15008 Un_KI270311v1 12399 Un_KI270366v1 8320 Un_KI270511v1 8127 Un_KI270448v1 7992 Un_KI270521v1 7642 Un_KI270581v1 7046 Un_KI270582v1 6504 Un_KI270515v1 6361 Un_KI270588v1 6158 Un_KI270591v1 5796 Un_KI270522v1 5674 Un_KI270507v1 5353 Un_KI270590v1 4685 Un_KI270584v1 4513 Un_KI270320v1 4416 Un_KI270382v1 4215 Un_KI270468v1 4055 Un_KI270467v1 3920 Un_KI270362v1 3530 Un_KI270517v1 3253 Un_KI270593v1 3041 Un_KI270528v1 2983 Un_KI270587v1 2969 Un_KI270364v1 2855 Un_KI270371v1 2805 Un_KI270333v1 2699 Un_KI270374v1 2656 Un_KI270411v1 2646 Un_KI270414v1 2489 Un_KI270510v1 2415 Un_KI270390v1 2387 Un_KI270375v1 2378 Un_KI270420v1 2321 Un_KI270509v1 2318 Un_KI270315v1 2276 Un_KI270302v1 2274 Un_KI270518v1 2186 Un_KI270530v1 2168 Un_KI270304v1 2165 Un_KI270418v1 2145 Un_KI270424v1 2140 Un_KI270417v1 2043 Un_KI270508v1 1951 Un_KI270303v1 1942 Un_KI270381v1 1930 Un_KI270529v1 1899 Un_KI270425v1 1884 Un_KI270396v1 1880 Un_KI270363v1 1803 Un_KI270386v1 1788 Un_KI270465v1 1774 Un_KI270383v1 1750 Un_KI270384v1 1658 Un_KI270330v1 1652 Un_KI270372v1 1650 Un_KI270548v1 1599 Un_KI270580v1 1553 Un_KI270387v1 1537 Un_KI270391v1 1484 Un_KI270305v1 1472 Un_KI270373v1 1451 Un_KI270422v1 1445 Un_KI270316v1 1444 Un_KI270338v1 1428 Un_KI270340v1 1428 Un_KI270583v1 1400 Un_KI270334v1 1368 Un_KI270429v1 1361 Un_KI270393v1 1308 Un_KI270516v1 1300 Un_KI270389v1 1298 Un_KI270466v1 1233 Un_KI270388v1 1216 Un_KI270544v1 1202 Un_KI270310v1 1201 Un_KI270412v1 1179 Un_KI270395v1 1143 Un_KI270376v1 1136 Un_KI270337v1 1121 Un_KI270335v1 1048 Un_KI270378v1 1048 Un_KI270379v1 1045 Un_KI270329v1 1040 Un_KI270419v1 1029 Un_KI270336v1 1026 Un_KI270312v1 998 Un_KI270539v1 993 Un_KI270385v1 990 Un_KI270423v1 981 Un_KI270392v1 971 Un_KI270394v1 970 ================================================ FILE: intro_awk_spring2021/data/Homo_sapiens.GRCh38.subset.gff3 ================================================ ##gff-version 3 ##sequence-region 1 1 248956422 1 Ensembl chromosome 1 248956422 . . . ID=chromosome:1 1 . biological_region 10469 11240 1.3e+03 . . external_name=oe %3D 0.79 1 . biological_region 10650 10657 0.999 + . logic_name=eponine 1 . biological_region 10655 10657 0.999 - . logic_name=eponine 1 . biological_region 10678 10687 0.999 + . logic_name=eponine 1 . biological_region 10681 10688 0.999 - . logic_name=eponine 1 . biological_region 10707 10716 0.999 + . logic_name=eponine 1 . biological_region 10708 10718 0.999 - . logic_name=eponine 1 . biological_region 10735 10747 0.999 - . logic_name=eponine 1 . biological_region 10737 10744 0.999 + . logic_name=eponine 1 . biological_region 10766 10773 0.999 + . logic_name=eponine 1 . biological_region 10770 10779 0.999 - . logic_name=eponine 1 . biological_region 10796 10801 0.999 + . logic_name=eponine 1 . biological_region 10810 10819 0.999 - . logic_name=eponine 1 . biological_region 10870 10872 0.999 + . logic_name=eponine 1 . biological_region 10889 10893 0.999 - . logic_name=eponine 1 havana pseudogene 11869 14409 . + . ID=gene:ENSG00000223972 1 havana lnc_RNA 11869 14409 . + . ID=transcript:ENST00000456328 1 havana exon 11869 12227 . + . Parent=transcript:ENST00000456328 1 havana exon 12613 12721 . + . Parent=transcript:ENST00000456328 1 havana exon 13221 14409 . + . Parent=transcript:ENST00000456328 1 havana pseudogenic_transcript 12010 13670 . + . ID=transcript:ENST00000450305 1 havana exon 12010 12057 . + . Parent=transcript:ENST00000450305 1 havana exon 12179 12227 . + . Parent=transcript:ENST00000450305 1 havana exon 12613 12697 . + . Parent=transcript:ENST00000450305 1 havana exon 12975 13052 . + . Parent=transcript:ENST00000450305 1 havana exon 13221 13374 . + . Parent=transcript:ENST00000450305 1 havana exon 13453 13670 . + . Parent=transcript:ENST00000450305 1 havana pseudogene 14404 29570 . - . ID=gene:ENSG00000227232 1 havana pseudogenic_transcript 14404 29570 . - . ID=transcript:ENST00000488147 1 havana exon 14404 14501 . - . Parent=transcript:ENST00000488147 1 havana exon 15005 15038 . - . Parent=transcript:ENST00000488147 1 havana exon 15796 15947 . - . Parent=transcript:ENST00000488147 1 havana exon 16607 16765 . - . Parent=transcript:ENST00000488147 1 havana exon 16858 17055 . - . Parent=transcript:ENST00000488147 1 havana exon 17233 17368 . - . Parent=transcript:ENST00000488147 1 havana exon 17606 17742 . - . Parent=transcript:ENST00000488147 1 havana exon 17915 18061 . - . Parent=transcript:ENST00000488147 1 havana exon 18268 18366 . - . Parent=transcript:ENST00000488147 1 havana exon 24738 24891 . - . Parent=transcript:ENST00000488147 1 havana exon 29534 29570 . - . Parent=transcript:ENST00000488147 1 . biological_region 15796 16060 0.999 - . external_name=rank %3D 1 1 mirbase ncRNA_gene 17369 17436 . - . ID=gene:ENSG00000278267 1 mirbase miRNA 17369 17436 . - . ID=transcript:ENST00000619216 1 mirbase exon 17369 17436 . - . Parent=transcript:ENST00000619216 1 . biological_region 28736 29810 1.01e+03 . . external_name=oe %3D 0.88 1 . biological_region 29116 29118 0.999 + . logic_name=eponine 1 . biological_region 29127 29206 1 + . external_name=rank %3D 1 1 . biological_region 29321 29395 1 - . external_name=rank %3D 1 1 . biological_region 29394 29396 0.999 - . logic_name=eponine 1 . biological_region 29448 29451 0.999 + . logic_name=eponine 1 havana ncRNA_gene 29554 31109 . + . ID=gene:ENSG00000243485 1 havana lnc_RNA 29554 31097 . + . ID=transcript:ENST00000473358 1 havana exon 29554 30039 . + . Parent=transcript:ENST00000473358 1 havana exon 30564 30667 . + . Parent=transcript:ENST00000473358 1 havana exon 30976 31097 . + . Parent=transcript:ENST00000473358 1 havana lnc_RNA 30267 31109 . + . ID=transcript:ENST00000469289 1 havana exon 30267 30667 . + . Parent=transcript:ENST00000469289 1 havana exon 30976 31109 . + . Parent=transcript:ENST00000469289 1 . biological_region 29583 29584 0.999 - . logic_name=eponine 1 mirbase ncRNA_gene 30366 30503 . + . ID=gene:ENSG00000284332 1 mirbase miRNA 30366 30503 . + . ID=transcript:ENST00000607096 1 mirbase exon 30366 30503 . + . Parent=transcript:ENST00000607096 1 havana ncRNA_gene 34554 36081 . - . ID=gene:ENSG00000237613 1 havana lnc_RNA 34554 36081 . - . ID=transcript:ENST00000417324 1 havana exon 34554 35174 . - . Parent=transcript:ENST00000417324 1 havana exon 35277 35481 . - . Parent=transcript:ENST00000417324 1 havana exon 35721 36081 . - . Parent=transcript:ENST00000417324 1 havana lnc_RNA 35245 36073 . - . ID=transcript:ENST00000461467 1 havana exon 35245 35481 . - . Parent=transcript:ENST00000461467 1 havana exon 35721 36073 . - . Parent=transcript:ENST00000461467 1 . biological_region 35904 36086 0.879 - . external_name=rank %3D 1 1 havana pseudogene 52473 53312 . + . ID=gene:ENSG00000268020 1 havana pseudogenic_transcript 52473 53312 . + . ID=transcript:ENST00000606857 1 havana exon 52473 53312 . + . Parent=transcript:ENST00000606857 1 havana pseudogene 57598 64116 . + . ID=gene:ENSG00000240361 1 havana lnc_RNA 57598 64116 . + . ID=transcript:ENST00000642116 1 havana exon 57598 57653 . + . Parent=transcript:ENST00000642116 1 havana exon 58700 58856 . + . Parent=transcript:ENST00000642116 1 havana exon 62916 64116 . + . Parent=transcript:ENST00000642116 1 havana pseudogenic_transcript 62949 63887 . + . ID=transcript:ENST00000492842 1 havana exon 62949 63887 . + . Parent=transcript:ENST00000492842 1 ensembl_havana gene 65419 71585 . + . ID=gene:ENSG00000186092 1 havana mRNA 65419 71585 . + . ID=transcript:ENST00000641515 1 havana exon 65419 65433 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 65419 65433 . + . Parent=transcript:ENST00000641515 1 havana exon 65520 65573 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 65520 65573 . + . Parent=transcript:ENST00000641515 1 havana five_prime_UTR 69037 69090 . + . Parent=transcript:ENST00000641515 1 havana exon 69037 71585 . + . Parent=transcript:ENST00000641515 1 havana CDS 69091 70008 . + 0 ID=CDS:ENSP00000493376 1 havana three_prime_UTR 70009 71585 . + . Parent=transcript:ENST00000641515 1 ensembl mRNA 69055 70108 . + . ID=transcript:ENST00000335137 1 ensembl five_prime_UTR 69055 69090 . + . Parent=transcript:ENST00000335137 1 ensembl exon 69055 70108 . + . Parent=transcript:ENST00000335137 1 ensembl CDS 69091 70008 . + 0 ID=CDS:ENSP00000334393 1 ensembl three_prime_UTR 70009 70108 . + . Parent=transcript:ENST00000335137 1 ensembl_havana ncRNA_gene 89295 133723 . - . ID=gene:ENSG00000238009 1 havana lnc_RNA 89295 120932 . - . ID=transcript:ENST00000466430 1 havana exon 89295 91629 . - . Parent=transcript:ENST00000466430 ================================================ FILE: intro_awk_spring2021/data/Homo_sapiens_ucscGenes.subset.bed ================================================ chr1 11873 14409 uc010nxq.1 chr1 14361 19759 uc009viu.3 chr1 14406 29370 uc009viw.2 chr1 34610 36081 uc001aak.3 chr1 69090 70008 uc001aal.1 chr1 134772 140566 uc021oeg.2 chr1 321083 321115 uc001aaq.2 chr1 321145 321207 uc001aar.2 chr1 322036 326938 uc009vjk.2 chr1 327545 328439 uc021oei.1 chr1 367658 368597 uc010nxu.2 chr1 420205 421839 uc001aax.1 chr1 566092 566115 uc021oej.1 chr1 566134 566155 uc021oek.1 chr1 566239 566263 uc021oel.1 chr1 568843 568913 uc001abb.3 chr1 621095 622034 uc010nxv.2 chr1 661138 670994 uc009vjm.3 chr1 668417 668479 uc001abi.2 chr1 668509 668541 uc001abj.3 chr1 671823 671885 uc010nxw.2 chr1 671915 671947 uc001abl.3 chr1 674239 679736 uc001abm.2 chr1 700244 714068 uc001abo.3 chr1 761585 762902 uc010nxx.2 chr1 762970 794826 uc001abp.2 chr1 803450 812182 uc001abt.4 chr1 846814 850328 uc001abu.1 chr1 852952 854817 uc010nxy.1 chr1 861120 879961 uc001abw.1 chr1 879582 894679 uc001abz.4 chr1 895966 901099 uc001aca.2 chr1 901876 910484 uc001acd.3 chr1 910578 917473 uc001ach.2 chr1 934341 935552 uc001aci.2 chr1 948846 949919 uc001acj.4 chr1 955502 991499 uc001ack.2 chr1 995116 1001833 uc001acl.1 chr1 1007125 1009687 uc021oen.1 chr1 1017197 1051736 uc001acu.2 chr1 1072396 1079434 uc001acv.3 chr1 1102483 1102578 uc001acw.2 chr1 1103242 1103332 uc010nye.1 chr1 1103295 1103317 uc031pkr.1 chr1 1104384 1104467 uc010nyf.1 chr1 1104434 1104456 uc031pks.1 chr1 1108435 1114935 uc001acx.1 chr1 1109285 1133313 uc001acy.2 chr1 1138887 1142089 uc001add.3 chr1 1146705 1149548 uc001ade.3 chr1 1152287 1167447 uc001adh.4 chr1 1167628 1170420 uc001adk.3 chr1 1177825 1182102 uc001adl.2 chr1 1189291 1209234 uc001ado.3 chr1 1215815 1227409 uc001adt.1 chr1 1227763 1243269 uc001aeb.2 chr1 1243993 1247057 uc001aed.3 chr1 1246964 1260067 uc001aee.2 chr1 1260142 1264276 uc001aeo.3 chr1 1266725 1269844 uc010nyk.2 chr1 1270657 1284492 uc001aer.4 chr1 1288070 1293915 uc001aew.3 chr1 1309109 1310562 uc009vkb.1 chr1 1321090 1334718 uc001afi.2 chr1 1334909 1337426 uc001afm.3 chr1 1337275 1342693 uc001afo.4 chr1 1353799 1356824 uc010nyo.2 chr1 1361507 1363167 uc010nyp.2 chr1 1370902 1378262 uc001afs.3 chr1 1385068 1405538 uc001aft.2 chr1 1407163 1431582 uc001afv.3 chr1 1447522 1470067 uc001afz.2 chr1 1470157 1475740 uc009vkf.3 chr1 1477052 1510262 uc001agd.3 chr1 1510354 1510644 uc021oer.1 chr1 1533387 1535476 uc021oes.1 chr1 1535818 1543166 uc001agf.1 chr1 1550794 1565990 uc001agg.3 chr1 1567559 1570030 uc001agp.3 chr1 1571099 1655775 uc001agv.1 chr1 1586822 1590469 uc001ahc.1 chr1 1592938 1624243 uc001ahg.4 chr1 1631377 1633247 uc001ahi.1 chr1 1656053 1663343 uc001ahx.2 chr1 1658823 1677438 uc001aia.2 chr1 1682670 1711508 uc001aie.3 chr1 1716724 1822526 uc001aif.3 chr1 1846265 1848733 uc001aih.1 chr1 1849028 1850740 uc001aij.2 chr1 1853395 1858842 uc001aik.3 chr1 1884751 1935276 uc001aim.1 chr1 1944651 1946969 uc001aio.1 chr1 1950767 1962192 uc001aip.2 chr1 1981908 2116834 uc001aiq.3 chr1 2112574 2114663 uc001aiu.1 chr1 2115898 2126214 uc031pkt.1 chr1 2121236 2123179 uc001aiz.2 chr1 2160133 2241652 uc001aja.4 chr1 2252695 2322993 uc001ajb.1 chr1 2281852 2284100 uc001ajc.3 chr1 2309493 2322993 uc010nyy.2 chr1 2323213 2336885 uc001aje.2 chr1 2336240 2344010 uc001ajg.3 chr1 2407753 2436964 uc001aji.1 chr1 2439974 2458035 uc001ajm.1 chr1 2460183 2461684 uc001ajn.3 chr1 2481358 2484284 uc001ajo.2 chr1 2486162 2488450 uc021oev.1 chr1 2487804 2495188 uc001ajt.1 chr1 2487804 2495267 uc001ajr.3 chr1 2518188 2522908 uc001ajv.2 chr1 2522080 2564481 uc001ajy.2 chr1 2572806 2706230 uc021oey.1 chr1 2938045 2939467 uc001ajz.3 chr1 2976180 2980350 uc001aka.3 chr1 2980635 2984289 uc010nzg.1 chr1 2985741 3355185 uc001akf.3 chr1 3044538 3044599 uc021oez.1 chr1 3371146 3397677 uc001akg.4 chr1 3404505 3528059 uc001akl.3 chr1 3477259 3477354 uc021ofa.1 chr1 3541555 3546694 uc001akm.3 chr1 3547330 3566671 uc001ako.3 chr1 3569128 3652765 uc001akp.3 chr1 3652547 3663937 uc009vlm.3 chr1 3668964 3688209 uc001akv.2 chr1 3689351 3692546 uc001akw.4 chr1 3696783 3713068 uc001akx.1 chr1 3728644 3773797 uc001aky.2 chr1 3773844 3801993 uc001alc.3 chr1 3805696 3816857 uc001alf.3 chr1 3816967 3832011 uc001alg.3 chr1 4000671 4012643 uc001ali.2 chr1 4472110 4484744 uc001alj.2 chr1 4715104 4843851 uc001aln.3 chr1 4847557 4852183 uc001alo.4 chr1 5621768 5728315 uc001alp.1 chr1 5624130 5624203 uc021ofm.1 chr1 5922731 5922801 uc021ofn.1 chr1 5922869 6052533 uc001alq.2 chr1 6105980 6161253 uc001aly.2 chr1 6161846 6240194 uc001amb.2 chr1 6245079 6259679 uc001amd.3 chr1 6266188 6281359 uc001amg.3 chr1 6281252 6296044 uc001amk.3 chr1 6297870 6299502 uc001amm.3 chr1 6304251 6305638 uc009vly.2 chr1 6307405 6321035 uc001amp.2 chr1 6324331 6453826 uc001amt.3 chr1 6475293 6479979 uc001amx.3 chr1 6484847 6521004 uc001amy.3 chr1 6489893 6489956 uc021ofo.1 chr1 6521213 6526255 uc001anh.3 chr1 6526151 6545529 uc010nzr.1 chr1 6581406 6614658 uc001ans.3 chr1 6615337 6639817 uc001ant.3 chr1 6640062 6649340 uc001anx.3 chr1 6650783 6662929 uc001aoa.3 chr1 6673755 6684093 uc001aob.4 chr1 6685209 6693642 uc001aod.3 chr1 6694227 6761966 uc001aof.2 chr1 6845383 7829766 uc001aoi.3 chr1 7831328 7841492 uc001aol.3 chr1 7844713 7905237 uc001aoo.3 chr1 7907671 7913565 uc001aos.3 chr1 7975930 8003225 uc001aot.3 chr1 7990338 7990408 uc021ofs.1 chr1 8021713 8045342 uc001aox.4 chr1 8071778 8086393 uc001aoz.3 chr1 8384389 8404227 uc001apb.3 chr1 8412463 8877699 uc001apf.3 chr1 8440651 8441235 uc001apg.1 chr1 8921058 8939151 uc001apj.2 chr1 8938893 8939943 uc021oft.1 chr1 9005892 9034503 uc031plc.1 chr1 9063358 9086404 uc009vmo.1 chr1 9097004 9129887 uc001apo.3 chr1 9164475 9189229 uc001apq.1 chr1 9186983 9189250 uc001aps.3 chr1 9208345 9242451 uc009vmq.3 chr1 9294862 9331394 uc001apt.3 chr1 9352940 9429590 uc010oae.2 chr1 9497727 9497837 uc021ofx.1 chr1 9599527 9642831 uc001apw.3 chr1 9648931 9674935 uc021ofy.1 chr1 9711789 9789172 uc001aqb.4 chr1 9712667 9714644 uc001aqc.4 chr1 9740902 9747627 uc021oga.1 chr1 9789078 9884550 uc001aqh.3 chr1 9908333 9970316 uc001aql.1 chr1 9989775 10002840 uc001aqm.3 chr1 10003485 10045556 uc001aqp.3 chr1 10057254 10076078 uc001aqq.3 chr1 10093040 10241296 uc001aqs.4 chr1 10270763 10441661 uc001aqw.4 chr1 10459084 10480201 uc001arc.3 chr1 10490158 10512060 uc021ogd.1 chr1 10520602 10532613 uc001arj.3 chr1 10535002 10690815 uc001arn.3 chr1 10696665 10856733 uc001aro.4 chr1 10744633 10744736 uc021ogh.1 chr1 11006529 11042094 uc010oao.2 chr1 11072678 11085549 uc001art.3 chr1 11086579 11107296 uc001aru.3 chr1 11114648 11120091 uc001arz.1 chr1 11126675 11159938 uc001asa.3 chr1 11166587 11322608 uc001asd.3 chr1 11203954 11209595 uc031plf.1 chr1 11249345 11256038 uc001ase.4 chr1 11333254 11348491 uc001asg.3 chr1 11539294 11597640 uc001ash.4 chr1 11708417 11714888 uc001asj.3 chr1 11714913 11723384 uc001asm.3 chr1 11724149 11734409 uc001aso.3 chr1 11734536 11751678 uc009vnc.3 chr1 11751780 11780336 uc001asr.1 chr1 11782186 11785914 uc001ass.2 chr1 11796141 11810828 uc001asv.3 chr1 11824461 11826573 uc001asy.1 chr1 11832138 11849642 uc001asz.3 chr1 11845786 11866160 uc001atc.2 chr1 11866152 11903201 uc001ate.5 chr1 11905766 11907840 uc001ati.3 chr1 11917520 11918992 uc001atj.3 chr1 11979644 11986485 uc001atk.3 chr1 11994723 12035599 uc001atm.3 chr1 12040237 12073572 uc009vni.3 chr1 12079298 12092106 uc001ato.2 chr1 12123433 12204264 uc001atq.3 chr1 12227059 12269277 uc001att.3 chr1 12251761 12251839 uc021ogi.1 chr1 12290112 12572098 uc001atv.3 chr1 12567299 12567451 uc001atz.1 chr1 12627938 12677820 uc001auc.3 chr1 12704565 12727097 uc001auf.3 chr1 12776117 12788726 uc009vnn.1 chr1 12806162 12821102 uc001auh.3 chr1 12834983 12838048 uc001aui.3 chr1 12851545 12856777 uc001auj.2 chr1 12884467 12891264 uc001auk.2 chr1 12907235 12908237 uc009vno.2 chr1 12916940 12921764 uc001aum.1 chr1 12939032 12946025 uc001aun.2 chr1 12952726 12958094 uc001auo.3 chr1 12976449 12980568 uc001aup.3 chr1 12998301 13007406 uc001auq.2 chr1 13035542 13038381 uc009vnq.1 chr1 13182959 13184326 uc010obg.2 chr1 13328195 13331692 uc001aut.1 chr1 13359818 13369057 uc001auu.1 chr1 13386646 13390765 uc001auv.3 chr1 13421175 13428191 uc001auw.1 chr1 13447413 13452656 uc010obi.1 chr1 13474052 13477569 uc009vnu.1 chr1 13495253 13498259 uc001aux.3 chr1 13516065 13526943 uc009vnv.1 chr1 13607430 13611550 uc001auy.2 chr1 13629937 13635299 uc001auz.4 chr1 13641972 13648988 uc001ava.1 chr1 13694888 13698405 uc009vny.1 chr1 13716087 13719064 uc009vnz.1 chr1 13736906 13747803 uc009voa.1 chr1 13801444 13840242 uc001avb.3 chr1 13910251 13944452 uc001avd.3 chr1 14031349 14151574 uc001avi.3 chr1 14925212 15444544 uc001avm.4 chr1 15438310 15478960 uc009voh.3 chr1 15480228 15546974 uc001avx.3 chr1 15573767 15724622 uc001awb.2 chr1 15653175 15670372 uc001awc.1 chr1 15736390 15756839 uc001awh.2 chr1 15764937 15773153 uc001awi.1 chr1 15783222 15798586 uc001awk.3 chr1 15802595 15817895 uc001awl.3 chr1 15817895 15850940 uc001awn.4 chr1 15853351 15898228 uc001aws.3 chr1 15898193 15911605 uc001awv.2 chr1 15943952 15987552 uc001awx.2 chr1 15986363 15988217 uc010obn.2 chr1 15992765 15995537 uc001awz.3 chr1 16010826 16061264 uc010obo.2 chr1 16062808 16067884 uc001axb.1 chr1 16068986 16074292 uc001axc.4 chr1 16085254 16113084 uc001axe.1 chr1 16133656 16134194 uc009vol.1 chr1 16160709 16174642 uc001axj.2 chr1 16174358 16266950 uc001axk.1 chr1 16268363 16302627 uc001axl.4 chr1 16317618 16317647 uc001axm.1 chr1 16330730 16333184 uc001axn.3 chr1 16340522 16345285 uc001axo.2 chr1 16348485 16360545 uc001axu.3 chr1 16384263 16400127 uc001axz.4 chr1 16450831 16482582 uc001aya.2 chr1 16524598 16539104 uc001ayc.1 chr1 16558181 16563659 uc001ayd.3 chr1 16576558 16678948 uc001ayg.3 chr1 16693524 16724643 uc001aym.5 chr1 16725137 16763919 uc001ayn.3 chr1 16767166 16786584 uc001ayq.3 chr1 16793930 16819196 uc001ayt.2 chr1 16847079 16847153 uc021ogp.1 chr1 16858892 16858966 uc021ogq.1 chr1 16860349 16862144 uc021ogr.1 chr1 16862254 16864669 uc001ayv.2 chr1 16872433 16872504 uc021ogs.1 chr1 16874159 16874232 uc021ogt.1 chr1 16875408 16875482 uc021ogu.1 chr1 16888921 16940100 uc001ayw.4 chr1 16944756 16959841 uc001azf.3 chr1 16972068 16976915 uc010och.2 chr1 17004765 17004836 uc021ogw.1 chr1 17006500 17006573 uc021ogx.1 chr1 17007749 17007823 uc021ogy.1 chr1 17017712 17046652 uc001azn.1 chr1 17052060 17052133 uc021ogz.1 chr1 17053779 17053850 uc021oha.1 chr1 17081128 17090975 uc010ock.3 chr1 17180899 17180971 uc021ohb.1 chr1 17185443 17185516 uc021ohc.1 chr1 17186692 17186765 uc021ohd.1 chr1 17188415 17188486 uc021ohe.1 chr1 17197439 17200574 uc021ohf.2 chr1 17201957 17202031 uc021ohg.1 chr1 17215040 17216161 uc001azs.1 chr1 17216171 17216245 uc021ohh.1 chr1 17222645 17222720 uc021ohi.1 chr1 17248444 17299474 uc001azt.2 chr1 17300998 17307173 uc001azw.3 chr1 17312452 17338423 uc001baa.2 chr1 17345224 17380665 uc001bae.3 chr1 17393255 17445948 uc001baf.3 chr1 17531620 17572501 uc001bah.1 chr1 17566189 17572501 uc009vpb.1 chr1 17575592 17610727 uc001bai.3 chr1 17581660 17581778 uc021ohk.1 chr1 17634689 17690495 uc001baj.2 chr1 17698740 17728195 uc001bak.1 chr1 17733250 17765059 uc001bal.3 chr1 17866329 18024370 uc001ban.3 chr1 18081807 18153558 uc001bat.3 chr1 18434239 18704977 uc001bau.2 chr1 18701063 18702174 uc001baw.1 chr1 18807423 18812480 uc001bax.3 chr1 18957499 19062632 uc001bay.3 chr1 19166092 19186155 uc001bba.1 chr1 19197923 19229293 uc001bbc.3 chr1 19209695 19209769 uc021ohm.2 chr1 19230773 19282826 uc001bbd.2 chr1 19400999 19536746 uc001bbi.3 chr1 19542157 19578053 uc001bbo.4 chr1 19578074 19586622 uc001bbs.3 chr1 19592475 19600568 uc021ohn.1 chr1 19609056 19615280 uc001bbv.1 chr1 19619740 19622230 uc021ohp.1 chr1 19629201 19638640 uc001bbw.3 chr1 19638739 19655794 uc001bby.3 chr1 19665266 19812066 uc021ohr.1 chr1 19673334 19675427 uc001bcf.2 chr1 19750877 19751182 uc021ohs.1 chr1 19923470 19956315 uc021ohu.1 chr1 19934300 19935138 uc021ohx.2 chr1 19969722 19984949 uc001bcj.2 chr1 19991779 20006055 uc001bcl.3 chr1 20008705 20126410 uc001bcn.3 chr1 20140521 20141771 uc001bcr.3 chr1 20208887 20239437 uc001bcs.4 chr1 20246799 20250110 uc001bct.1 chr1 20301923 20306932 uc010odb.2 chr1 20396700 20418394 uc001bcy.3 chr1 20439142 20446059 uc001bcz.4 chr1 20465822 20476879 uc009vpp.1 chr1 20490483 20501687 uc009vpq.1 chr1 20512577 20519942 uc001bdb.3 chr1 20617411 20681387 uc009vps.2 chr1 20686293 20755287 uc001bdf.2 chr1 20808883 20812728 uc001bdh.3 chr1 20825940 20834674 uc001bdi.4 chr1 20878931 20881513 uc001bdj.3 chr1 20915443 20945400 uc001bdk.3 chr1 20959947 20978004 uc001bdm.3 chr1 20978259 20988037 uc001bdo.1 chr1 20990506 21044317 uc001bdr.4 chr1 21046224 21059133 uc009vpy.1 chr1 21069170 21113181 uc001bdw.1 chr1 21132784 21503381 uc001bef.3 chr1 21543739 21616766 uc001bei.2 chr1 21602542 21604868 uc001ben.1 chr1 21619782 21626362 uc001beo.2 chr1 21749600 21754300 uc001bep.1 chr1 21761832 21762609 uc001beq.1 chr1 21766582 21811393 uc001ber.4 chr1 21835857 21904905 uc001bet.3 chr1 21922707 21978348 uc001bew.3 chr1 22004791 22109688 uc001bfb.3 chr1 22138757 22151714 uc001bfg.1 chr1 22148736 22263750 uc001bfj.3 chr1 22303417 22315847 uc001bfk.3 chr1 22351706 22357715 uc001bfm.4 chr1 22379119 22419436 uc001bfr.3 chr1 22443797 22469519 uc001bfs.4 chr1 22778343 22857650 uc001bfu.2 chr1 22890003 22930087 uc001bfx.1 chr1 22963117 22966175 uc001bfy.3 chr1 22970117 22974603 uc001bga.4 chr1 22979681 22988029 uc001bgd.3 chr1 23037330 23241823 uc001bge.3 chr1 23046009 23046091 uc021oib.1 chr1 23189651 23189719 uc021oic.1 chr1 23243782 23247347 uc001bgg.1 chr1 23337326 23342343 uc001bgh.1 chr1 23345940 23410184 uc001bgj.2 chr1 23370797 23370865 uc021oid.1 chr1 23410515 23495517 uc010odv.1 chr1 23490445 23490546 uc021oie.1 chr1 23518387 23521222 uc001bgn.3 chr1 23636275 23670853 uc001bgp.4 chr1 23685940 23694879 uc001bgt.3 chr1 23695463 23698330 uc001bgw.3 chr1 23707554 23751261 uc021oig.1 chr1 23755055 23810750 uc001bha.2 chr1 23801092 23803135 uc001bhd.4 chr1 23832919 23857712 uc001bhe.2 chr1 23853364 23855542 uc001bhf.1 chr1 23884420 23886285 uc001bhh.4 chr1 23907984 23967056 uc001bhi.3 chr1 24018268 24022915 uc001bhk.3 chr1 24069855 24088549 uc001bho.3 chr1 24086871 24104787 uc001bhp.2 chr1 24104875 24114722 uc001bhq.3 chr1 24117645 24122029 uc001bht.3 chr1 24122088 24126060 uc009vqo.1 chr1 24128366 24151949 uc001bib.3 chr1 24171571 24194859 uc001bie.3 chr1 24200459 24239817 uc001bif.3 chr1 24255559 24255637 uc021oik.1 chr1 24286300 24289949 uc001big.3 chr1 24295572 24306953 uc021oir.1 chr1 24320925 24320957 uc021oit.1 chr1 24382530 24438665 uc001bin.4 chr1 24446260 24469775 uc001biq.2 chr1 24480646 24513765 uc001bis.3 chr1 24526729 24538180 uc010oei.1 chr1 24578722 24578758 uc021oiu.1 chr1 24579767 24579803 uc021oiv.1 chr1 24645811 24690970 uc021oiw.1 chr1 24683488 24740262 uc001bjc.3 chr1 24742244 24799473 uc001bjh.3 chr1 24822822 24828850 uc021oiz.1 chr1 24829386 24863510 uc001bjj.3 chr1 24882566 24935818 uc001bjk.2 chr1 24969593 24999772 uc001bjm.3 chr1 25071759 25170815 uc001bjo.2 chr1 25226001 25256770 uc001bjq.3 chr1 25548766 25559013 uc001bjt.1 chr1 25568739 25573985 uc001bjw.3 chr1 25598980 25656936 uc001bjz.3 chr1 25629228 25631643 uc001bkd.1 chr1 25664788 25688852 uc001bke.3 chr1 25688739 25747363 uc001bkf.3 chr1 25757387 25826698 uc001bkk.3 chr1 25870075 25895377 uc001bkl.4 chr1 25943958 26111258 uc001bkm.2 chr1 26126666 26144713 uc021ojl.1 chr1 26146396 26159433 uc001bkq.4 chr1 26146444 26150097 uc010oeu.1 chr1 26160496 26185848 uc001bkw.1 chr1 26187974 26197744 uc001bkx.3 chr1 26210676 26232993 uc010oev.2 chr1 26286257 26324648 uc001bld.4 chr1 26348270 26362954 uc001blf.3 chr1 26364513 26372604 uc001blg.1 chr1 26377795 26394125 uc001bli.2 chr1 26438267 26452039 uc009vsb.3 chr1 26485510 26489119 uc001blk.3 chr1 26496387 26497364 uc001bll.4 chr1 26503980 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chr1 40627040 40706593 uc001cfc.4 chr1 40713572 40717365 uc001cfe.2 chr1 40723721 40759856 uc001cfg.4 chr1 40766162 40782981 uc001cfh.1 chr1 40839377 40888998 uc001cfj.3 chr1 40916336 40929390 uc001cfn.2 chr1 40943301 40962015 uc001cfo.3 chr1 40974432 40982214 uc001cfp.3 chr1 40997232 41013841 uc001cft.2 chr1 41086351 41131324 uc001cfu.1 chr1 41154751 41157933 uc010ojl.1 chr1 41157241 41237275 uc009vwd.3 chr1 41220026 41220118 uc001cgf.2 chr1 41222955 41223044 uc001cgg.3 chr1 41249683 41306124 uc001cgh.2 chr1 41326727 41328018 uc001cgj.3 chr1 41347313 41347427 uc021omc.1 chr1 41445006 41478235 uc001cgk.4 chr1 41480261 41509562 uc021omd.1 chr1 41481268 41487427 uc001cgm.2 chr1 41492870 41707815 uc001cgs.3 chr1 41932607 41932699 uc021ome.1 chr1 41944445 41949874 uc009vwh.3 chr1 41944445 41950344 uc001cgx.3 chr1 41972035 42384496 uc001cha.4 chr1 42619091 42621495 uc001chc.1 chr1 42628361 42630395 uc001chd.1 chr1 42642209 42800903 uc001chf.3 chr1 42846467 42889900 uc001chi.2 chr1 42896000 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chr1 45287937 45308616 uc010olf.2 chr1 45316193 45452394 uc001cmt.3 chr1 45468219 45477027 uc009vxk.3 chr1 45477804 45481341 uc001cna.2 chr1 45482075 45672250 uc001cnd.2 chr1 45769581 45771291 uc010olk.2 chr1 45792544 45794346 uc001cne.3 chr1 45794913 45806142 uc009vxp.3 chr1 45805341 45809650 uc009vxq.3 chr1 45809554 45956840 uc001cns.1 chr1 45959597 45965751 uc001cnw.3 chr1 45965855 45976739 uc009vxv.3 chr1 45976706 45988562 uc021omw.1 chr1 46016454 46035723 uc001coe.3 chr1 46049659 46084578 uc001coi.2 chr1 46085715 46089731 uc010olt.2 chr1 46092975 46152302 uc001coq.3 chr1 46111451 46112357 uc010olu.1 chr1 46153846 46160108 uc001cor.1 chr1 46164406 46216485 uc001cou.3 chr1 46269284 46501796 uc001cov.3 chr1 46505811 46598380 uc001cpb.4 chr1 46640748 46651634 uc001cpd.3 chr1 46654352 46685977 uc001cpg.3 chr1 46669005 46686928 uc010oma.2 chr1 46713366 46744145 uc009vye.2 chr1 46744071 46769038 uc001cpn.3 chr1 46769379 46782447 uc001cpp.3 chr1 46805848 46830824 uc001cpr.2 chr1 46859938 46879520 uc001cpu.2 chr1 46899498 46911374 uc021ona.1 chr1 46972667 46979886 uc001cpx.3 chr1 47004367 47035927 uc021onb.1 chr1 47011315 47015678 uc001cpy.2 chr1 47011315 47016887 uc009vyh.1 chr1 47023078 47069966 uc001cqb.4 chr1 47073386 47080805 uc001cqe.4 chr1 47100710 47134099 uc001cqh.4 chr1 47137496 47139256 uc001cqj.3 chr1 47139707 47157769 uc021ond.1 chr1 47140830 47184736 uc001cqk.4 chr1 47264669 47285021 uc001cqn.4 chr1 47308766 47366147 uc031pmm.1 chr1 47394845 47407156 uc001cqp.4 chr1 47489239 47516423 uc001cqt.3 chr1 47533159 47583992 uc001cqu.1 chr1 47603106 47614526 uc001cqv.1 chr1 47644921 47646011 uc031pmn.1 chr1 47649260 47655771 uc001cqw.3 chr1 47681962 47689770 uc009vyq.2 chr1 47681962 47695443 uc001cqx.2 chr1 47691628 47691655 uc021onf.1 chr1 47715810 47779819 uc001crd.1 chr1 47799468 47844511 uc001cri.3 chr1 47859449 47861215 uc001crj.1 chr1 47881743 47883724 uc001crk.3 chr1 47897806 47900313 uc001crl.3 chr1 47901688 47906363 uc001crm.3 chr1 48226199 48462562 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chr1 53099065 53122737 uc001cuf.3 chr1 53152013 53164038 uc001cui.2 chr1 53192130 53293013 uc001cuj.3 chr1 53308182 53360247 uc001cuk.2 chr1 53361581 53387591 uc001cup.4 chr1 53392900 53517289 uc001cur.2 chr1 53527723 53551174 uc010onr.2 chr1 53552854 53608289 uc001cuy.3 chr1 53580247 53584281 uc001cva.1 chr1 53662100 53679869 uc001cvb.4 chr1 53679771 53686289 uc001cvd.3 chr1 53692563 53704282 uc001cvf.2 chr1 53704281 53708455 uc001cvg.3 chr1 53708040 53793821 uc001cvi.2 chr1 53793904 53802889 uc001cvn.1 chr1 53904042 53905693 uc009vzj.3 chr1 53925071 53933158 uc001cvq.1 chr1 53971905 54199877 uc001cvr.1 chr1 54231133 54304225 uc001cvs.3 chr1 54317391 54355487 uc001cvu.3 chr1 54359860 54376759 uc001cwb.3 chr1 54387233 54411288 uc001cwh.3 chr1 54411998 54433841 uc001cwj.2 chr1 54472970 54483859 uc001cwm.2 chr1 54497348 54519111 uc001cwp.3 chr1 54519273 54565416 uc001cwt.1 chr1 54519751 54519827 uc021ons.1 chr1 54604667 54618679 uc001cwv.2 chr1 54638026 54665746 uc009vzo.3 chr1 54665839 54684056 uc001cxa.4 chr1 54691103 54872068 uc001cxe.4 chr1 55013806 55076005 uc001cxl.2 chr1 55074849 55089200 uc001cxn.3 chr1 55107426 55175939 uc010ooe.1 chr1 55181494 55208328 uc001cxx.4 chr1 55222570 55230226 uc001cxy.3 chr1 55246751 55266941 uc009vzt.1 chr1 55271735 55307937 uc001cyb.4 chr1 55315299 55352921 uc001cyc.1 chr1 55352654 55353883 uc021onu.1 chr1 55423541 55423614 uc021onv.1 chr1 55446464 55457966 uc001cyd.3 chr1 55464616 55474465 uc001cye.3 chr1 55505148 55530526 uc001cyf.2 chr1 55532031 55681039 uc021onw.1 chr1 55681080 55683128 uc021onx.1 chr1 55691313 55691396 uc021ony.1 chr1 55842198 55842525 uc021onz.1 chr1 55950543 55950645 uc021ooa.1 chr1 56046709 56200675 uc001cyi.1 chr1 56960418 57045257 uc001cyj.2 chr1 57110989 57181008 uc001cyk.4 chr1 57184476 57285369 uc001cym.4 chr1 57289353 57292593 uc001cyn.3 chr1 57320442 57383894 uc001cyo.2 chr1 57394882 57431688 uc001cyp.3 chr1 57463578 58716211 uc001cys.1 chr1 58326214 58328786 uc001cyu.1 chr1 58933598 58934677 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chr1 92495532 92529093 uc001don.2 chr1 92545861 92613401 uc001doo.3 chr1 92632608 92650280 uc010otd.2 chr1 92683572 92711367 uc001doq.3 chr1 92711954 92764566 uc001dor.3 chr1 92764521 92853732 uc001dot.2 chr1 92940317 92951628 uc001dov.4 chr1 92974252 93257961 uc001dox.3 chr1 93297593 93307481 uc001doz.3 chr1 93302845 93302940 uc001dpe.2 chr1 93303574 93303627 uc021opt.1 chr1 93307716 93427079 uc001dpg.3 chr1 93544791 93604638 uc009wdj.3 chr1 93615298 93646246 uc001dpn.3 chr1 93646280 93744287 uc021opx.1 chr1 93775665 93811368 uc001dpt.2 chr1 93811477 93828148 uc001dpu.3 chr1 93913687 94020218 uc010otk.2 chr1 93981833 93981906 uc021opz.1 chr1 94027342 94146926 uc001dpz.4 chr1 94057524 94065587 uc009wdn.3 chr1 94219111 94240930 uc001dqd.1 chr1 94312387 94312467 uc021oqa.1 chr1 94313128 94313213 uc021oqb.1 chr1 94317792 94319887 uc001dqe.1 chr1 94335013 94344762 uc001dqf.3 chr1 94352589 94375012 uc001dqg.1 chr1 94458393 94586705 uc001dqh.3 chr1 94634462 94703307 uc001dqj.4 chr1 94883932 94984219 uc001dqn.4 chr1 94994731 95007413 uc001dqr.3 chr1 95123088 95285834 uc001dqu.3 chr1 95285897 95360803 uc001dqv.5 chr1 95362506 95392735 uc001dqz.4 chr1 95393583 95428826 uc021oqc.2 chr1 95448278 95538507 uc001dra.2 chr1 95550007 95550119 uc021oqd.1 chr1 95582893 95663161 uc001drb.3 chr1 95628774 95699538 uc001dre.1 chr1 95699710 95712781 uc009wdu.3 chr1 95940292 95944912 uc021oqf.1 chr1 95970531 95970615 uc021oqg.1 chr1 95975895 95981020 uc001drl.3 chr1 97161411 97161723 uc021oqh.1 chr1 97187174 97280605 uc001drq.3 chr1 97543299 98386615 uc001drv.3 chr1 97561478 97788511 uc031pne.1 chr1 98453555 98515249 uc001drx.2 chr1 98510798 98510907 uc021oqj.1 chr1 98676266 98738214 uc031pnf.1 chr1 99127235 99226056 uc010ouc.2 chr1 99207463 99207540 uc021oql.1 chr1 99355800 99470449 uc001dsb.3 chr1 99469831 99614408 uc001dsd.1 chr1 99729847 99775138 uc001dse.3 chr1 100111430 100160097 uc001dsg.3 chr1 100174258 100231349 uc001dsh.1 chr1 100178485 100178513 uc021oqn.1 chr1 100178485 100178513 uc021oqm.1 chr1 100315639 100389579 uc001dsi.1 chr1 100434000 100435404 uc001dso.2 chr1 100435991 100492534 uc001dsr.2 chr1 100503788 100548929 uc001dst.3 chr1 100549101 100598511 uc001dsu.3 chr1 100598705 100616054 uc001dsv.3 chr1 100614003 100643829 uc001dsx.2 chr1 100652477 100715409 uc001dta.3 chr1 100731713 100758325 uc001dtd.3 chr1 100746796 100746864 uc021oqp.1 chr1 100818022 100965021 uc001dtf.2 chr1 101003727 101007583 uc001dth.3 chr1 101092605 101112560 uc021oqr.1 chr1 101185195 101204601 uc001dti.3 chr1 101337927 101360735 uc001dtk.2 chr1 101361631 101447311 uc001dto.2 chr1 101455179 101491362 uc001dtt.2 chr1 101491408 101552819 uc001dua.3 chr1 101702304 101707076 uc001dud.2 chr1 101746453 101746573 uc021oqu.1 chr1 101806424 101806451 uc031pni.1 chr1 102268126 102462790 uc001dug.2 chr1 102337566 102360299 uc021oqv.1 chr1 103342022 103574052 uc001dul.3 chr1 104068577 104097859 uc010oun.2 chr1 104095895 104122149 uc001duq.3 chr1 104159998 104168400 uc001dut.3 chr1 104198301 104207173 uc001duv.3 chr1 104230039 104238912 uc001duw.1 chr1 104257374 104262492 uc010our.1 chr1 104292439 104301311 uc001duz.3 chr1 104615644 104619693 uc021oqw.1 chr1 106144773 106161557 uc001dva.3 chr1 107599266 107601916 uc010ous.2 chr1 107682628 108024475 uc001dvh.4 chr1 107937775 108024475 uc001dvi.3 chr1 107937775 108024475 uc009wem.3 chr1 108113781 108507545 uc001dvk.1 chr1 108496274 108496345 uc021oqx.1 chr1 108507064 108537229 uc031pnj.1 chr1 108677343 108742980 uc001dvn.5 chr1 108765962 108786703 uc009weo.2 chr1 108803819 108816311 uc001dvp.2 chr1 108918459 108953434 uc001dvq.3 chr1 108963310 108975804 uc001dvr.2 chr1 108992903 109013260 uc009wep.3 chr1 109102970 109181949 uc010ouy.2 chr1 109190909 109203744 uc001dvt.4 chr1 109234931 109244422 uc001dvv.4 chr1 109255555 109285367 uc001dvx.3 chr1 109289284 109352148 uc001dvy.3 chr1 109358519 109399726 uc001dwa.4 chr1 109399838 109401146 uc001dwc.3 chr1 109419602 109473044 uc010ovc.2 chr1 109472129 109506121 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110473616 uc001dyw.4 chr1 110527386 110566364 uc001dyx.3 chr1 110577222 110597424 uc001dza.2 chr1 110602996 110613322 uc001dzb.3 chr1 110603303 110603368 uc021orl.1 chr1 110655061 110656569 uc001dzc.3 chr1 110693131 110744823 uc009wfq.3 chr1 110753335 110776674 uc001dzh.3 chr1 110815105 110815229 uc021orm.1 chr1 110828998 110881793 uc001dzj.3 chr1 110881944 110889303 uc001dzl.1 chr1 110887099 110888734 uc001dzn.1 chr1 110905472 110933704 uc001dzo.2 chr1 110943876 110950546 uc001dzr.3 chr1 110950430 110958896 uc031pnm.1 chr1 110993787 110999976 uc001dzs.3 chr1 111023387 111033891 uc009wfu.1 chr1 111059838 111061797 uc001dzt.1 chr1 111145775 111148975 uc009wfw.3 chr1 111214309 111217655 uc001dzv.1 chr1 111413820 111442558 uc001dzw.3 chr1 111489811 111506566 uc001eaa.3 chr1 111659953 111682838 uc001ead.4 chr1 111682248 111727724 uc001eah.1 chr1 111728590 111747160 uc001eal.2 chr1 111770280 111786062 uc001eam.3 chr1 111823145 111828730 uc009wgb.3 chr1 111833473 111863188 uc001eas.4 chr1 111889194 111895639 uc001eaw.2 chr1 111927140 111932473 uc021orp.1 chr1 111956936 111970399 uc001eba.3 chr1 111982511 111991830 uc001ebb.3 chr1 111991742 112004525 uc001ebc.3 chr1 112016603 112021134 uc001ebe.3 chr1 112025969 112046743 uc001ebf.3 chr1 112032938 112033045 uc021orr.1 chr1 112141628 112150940 uc001ebj.2 chr1 112162404 112256101 uc001ebl.3 chr1 112256829 112259310 uc001ebn.1 chr1 112264685 112282046 uc001ebo.2 chr1 112282462 112290420 uc001ebq.1 chr1 112287934 112298131 uc001ebr.3 chr1 112298189 112310199 uc001ebs.3 chr1 112318453 112531777 uc001ebu.1 chr1 112533189 112541463 uc001ebw.4 chr1 112913625 112913729 uc021oru.1 chr1 112938799 113003786 uc001ebx.3 chr1 112938880 112941439 uc001eby.1 chr1 113004391 113004455 uc021orv.1 chr1 113051369 113063910 uc001ecb.3 chr1 113066140 113162040 uc001ecd.3 chr1 113162074 113214241 uc001ecj.1 chr1 113217047 113243368 uc001eck.3 chr1 113243748 113249678 uc001ecp.1 chr1 113252615 113257950 uc001ect.1 chr1 113263188 113269856 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235093089 235099746 uc001hwk.4 chr1 235272657 235292256 uc001hwl.3 chr1 235291117 235291252 uc001hwm.1 chr1 235294497 235324772 uc001hwn.3 chr1 235330209 235491532 uc001hwq.3 chr1 235353348 235353431 uc021pkt.1 chr1 235491752 235507844 uc001hwv.3 chr1 235530727 235612280 uc001hxa.1 chr1 235610504 235667781 uc001hxc.3 chr1 235710984 235814054 uc001hxh.4 chr1 235712454 235714587 uc021pku.1 chr1 235824330 236030227 uc001hxj.3 chr1 236016299 236016360 uc021pkv.1 chr1 236139131 236228481 uc001hxo.3 chr1 236227659 236229779 uc001hxp.1 chr1 236305831 236372209 uc001hxq.3 chr1 236378421 236445339 uc001hxt.3 chr1 236557679 236648008 uc001hxu.1 chr1 236686368 236687808 uc001hxx.3 chr1 236686738 236716279 uc001hxy.2 chr1 236712304 236767841 uc001hyd.2 chr1 236849769 236927558 uc001hyf.2 chr1 236958580 237067281 uc001hyi.4 chr1 236973802 236992568 uc009xgj.1 chr1 237167402 237167718 uc001hyk.2 chr1 237205701 237997288 uc001hyl.1 chr1 237284106 237284409 uc021pkw.1 chr1 238025474 238091619 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243729741 uc021plv.1 chr1 244080703 244210619 uc001iac.3 chr1 244212240 244220780 uc031psv.1 chr1 244515936 244552388 uc001iah.4 chr1 244571793 244615436 uc001iaj.3 chr1 244624672 244803662 uc001iam.3 chr1 244816351 244872334 uc001iao.3 chr1 244998638 245008359 uc001iar.3 chr1 245003939 245010243 uc001iav.3 chr1 245013601 245027827 uc001iaz.1 chr1 245133170 245251148 uc001ibc.2 chr1 245318286 245866428 uc001ibf.1 chr1 245533635 245533719 uc021plw.1 chr1 245912641 246670644 uc001ibl.3 chr1 246679340 246687589 uc021plx.1 chr1 246703862 246729565 uc001ibn.3 chr1 246729638 246831884 uc001ibp.3 chr1 246887377 246931440 uc001ibr.3 chr1 246939314 246955685 uc001ibs.1 chr1 246970416 246970487 uc021ply.1 chr1 247002401 247094726 uc001ibv.2 chr1 247148624 247171395 uc009xgu.3 chr1 247197939 247242115 uc001icd.2 chr1 247263263 247267674 uc001ice.2 chr1 247273461 247275719 uc001icg.1 chr1 247319202 247335318 uc001icj.1 chr1 247337903 247338870 uc001icl.1 chr1 247365268 247365362 uc021pma.1 chr1 247419373 247420447 uc010pyu.2 chr1 247463621 247495045 uc001ico.3 chr1 247579457 247612406 uc001icr.3 chr1 247614330 247615284 uc010pyx.2 chr1 247654369 247655711 uc001icz.2 chr1 247681594 247683942 uc001idc.3 chr1 247693433 247697141 uc009xgy.3 chr1 247712346 247739848 uc001idf.3 chr1 247751661 247752615 uc010pyy.2 chr1 247768887 247769817 uc010pyz.2 chr1 247835419 247836343 uc001idi.1 chr1 247875130 247876057 uc001idj.1 chr1 247920763 247921708 uc010pza.2 chr1 247978101 247979031 uc001idm.1 chr1 248004229 248005198 uc001idn.1 chr1 248020500 248043438 uc001ido.3 chr1 248058888 248059833 uc010pzb.2 chr1 248084319 248085258 uc010pzc.2 chr1 248100492 248264224 uc001ids.3 chr1 248112159 248113098 uc001idt.1 chr1 248128633 248129641 uc010pzd.2 chr1 248153568 248154493 uc001idv.1 chr1 248185249 248186188 uc031psy.1 chr1 248201473 248202607 uc001idw.3 chr1 248223983 248224922 uc001idx.1 chr1 248285437 248286082 uc001idy.1 chr1 248308449 248309388 uc010pze.2 chr1 248343287 248344331 uc010pzf.2 chr1 248366369 248367308 uc010pzg.2 chr1 248402230 248403166 uc010pzh.2 chr1 248436153 248437116 uc010pzi.2 chr1 248457917 248458880 uc010pzj.2 chr1 248486931 248487870 uc010pzk.2 chr1 248512076 248513015 uc010pzl.2 chr1 248524882 248525929 uc001ieh.1 chr1 248550909 248551836 uc001iei.1 chr1 248569295 248570405 uc010pzm.2 chr1 248616098 248617073 uc001iek.1 chr1 248636651 248637608 uc001iel.1 chr1 248651889 248652837 uc001iem.1 chr1 248684947 248685898 uc001ien.1 chr1 248721844 248722792 uc001ieo.2 chr1 248737101 248738058 uc001iep.1 chr1 248756130 248757069 uc010pzn.2 chr1 248789478 248790429 uc001ier.1 chr1 248801587 248802559 uc001ies.1 chr1 248813231 248814185 uc010pzo.2 chr1 248844669 248845605 uc001ieu.1 chr1 248881947 248885507 uc031psz.1 chr1 248902716 248903151 uc009xhb.3 chr1 249104650 249120154 uc001iew.1 chr1 249120575 249120642 uc021pmd.1 chr1 249132529 249143714 uc001iex.3 chr1 249144202 249153315 uc001ifc.2 chr1 249168053 249168159 uc021pmf.1 chr1 249168446 249168518 uc021pmg.1 chr1 249200441 249213345 uc001ifh.3 ================================================ FILE: intro_awk_spring2021/data/dmel-subset-chromosomes.fasta ================================================ >X GAATTCGTCAGAAATGAGCTAAACAAATTTAAATCATTAAATGCGAGCGGCGAATCCGGAAACAGCAACTTCAAACCAGT >2L CGACAATGCACGACAGAGGAAGCAGAACAGATATTTAGATTGCCTCTCATTTTCTCTCCCATATTATAGGGAGAAATATG >2R CTCAAGATACCTTCTACAGATTATTTAAAGCTAGTGCACAACAACAATAAATTGACTAAGTTATGTCATTTTAAGCGGTC >3L TAGGGAGAAATATGATCGCGTATGCGAGAGTAGTGCCAACATATTGTGCTCTTTGATTTTTTGGCAACCCAAAATGGTGG >3R ACGGGACCGAGTATAGTACCAGTACGCGACCAGTACGGGAGCAGTACGGAACCAATACGGGTCCAGTACGGGTCCAGTAC ================================================ FILE: intro_awk_spring2021/data/enhancers.csv ================================================ chr1,1015066,1015266,HSE897,9.5706324138 chr1,1590473,1590673,HSE853,17.3206898329 chr1,2120861,2121064,HSE86,66.0424471614 chr1,6336418,6336624,HSE394,14.8892086906 chr1,7404594,7404794,HSE315,24.228051023 chr1,11941325,11941525,HSE322,17.5192630328 chr1,15055555,15055755,HSE962,11.7065788965 chr1,15478024,15478224,HSE354,17.5771586196 chr1,16065307,16065508,HSE264,23.9092446094 chr1,23244249,23244449,HSE434,17.1331422162 chr1,24621426,24621626,HSE638,11.9517576968 chr1,24710189,24710389,HSE537,14.6283754801 chr1,26216838,26217042,HSE237,39.9988740347 chr1,28931703,28931903,HSE150,37.3158468645 chr1,29139636,29139836,HSE696,9.3933913885 chr1,29301011,29301211,HSE837,15.2711109524 chr1,30644209,30644410,HSE452,13.1331424716 chr1,37323436,37323636,HSE144,24.6337166989 chr1,39114092,39114292,HSE642,13.5250552809 chr1,40887095,40887295,HSE715,14.4526764769 chr1,43259581,43259781,HSE285,23.3546182652 chr1,48180470,48180671,HSE581,14.0513005602 chr1,51911123,51911323,HSE728,10.9727627129 chr1,58868480,58868680,HSE744,10.9393507729 chr1,61907580,61907780,HSE219,24.9065022738 chr1,62649216,62649416,HSE160,31.6287882019 chr1,64297272,64297472,HSE542,16.032234097 chr1,71842002,71842202,HSE525,13.177872984 chr1,76529679,76529879,HSE217,28.2285409421 chr1,77034610,77034811,HSE117,36.8686336226 chr1,77861083,77861283,HSE95,33.1051379887 chr1,81139876,81140076,HSE648,14.2447596506 chr1,84078697,84078897,HSE229,19.6626071807 chr1,85040012,85040217,HSE554,18.2435809591 chr1,85294189,85294389,HSE649,12.4198070103 chr1,86952858,86953058,HSE227,20.0246313683 chr1,87198821,87199021,HSE396,14.181885534 chr1,87568855,87569055,HSE187,27.2483801299 chr1,87718281,87718481,HSE761,10.7346086388 chr1,90228438,90228639,HSE725,10.8919859879 chr1,90567852,90568052,HSE750,11.3840777324 chr1,101544928,101545128,HSE344,15.9279633229 chr1,105180209,105180409,HSE319,15.9878097581 chr1,112056258,112056458,HSE234,20.4981560995 chr1,113265359,113265559,HSE675,15.7764663556 chr1,120009313,120009513,HSE287,17.3492427166 chr1,120096931,120097131,HSE391,16.3648875858 chr1,145474303,145474503,HSE568,15.9686715881 chr1,146037814,146038014,HSE867,8.3915764216 chr1,149294554,149294754,HSE758,13.8367903871 chr1,149298520,149298734,HSE549,19.9633772792 chr1,161893534,161893734,HSE511,14.2318525715 chr1,165509248,165509448,HSE683,13.7673640003 chr1,167774457,167774657,HSE42,47.5183173 chr1,170555465,170555665,HSE348,25.4137901578 chr1,171264616,171264816,HSE405,18.0971419626 chr1,182113250,182113450,HSE687,17.1842942374 chr1,182635108,182635309,HSE247,28.4610896172 chr1,183573285,183573486,HSE44,36.1099998528 chr1,183681289,183681489,HSE77,46.3313570709 chr1,190550474,190550674,HSE461,24.1417645601 chr1,192030096,192030296,HSE839,8.898236823 chr1,192602518,192602718,HSE747,12.1928710846 chr1,193387547,193387747,HSE251,22.6738118926 chr1,201469917,201470118,HSE286,21.167260695 chr1,205411096,205411296,HSE399,15.4863010561 chr1,207682168,207682368,HSE389,14.4420873863 chr1,208751067,208751267,HSE250,28.3356979117 chr1,211556044,211556244,HSE913,10.1071053671 chr1,215227649,215227849,HSE643,12.2289936805 chr1,222946396,222946596,HSE136,28.3288830309 chr1,224716721,224716921,HSE669,11.1939548835 chr1,224767352,224767552,HSE659,12.7072133153 chr1,229007236,229007436,HSE259,25.6576825275 chr1,231831560,231831760,HSE103,34.1933527171 chr1,234707634,234707834,HSE458,16.2547381587 chr1,240192775,240192976,HSE192,23.6438756614 chr1,240751207,240751408,HSE41,39.1665180557 chr1,242513807,242514007,HSE29,41.8646049022 chr1,243428176,243428376,HSE909,8.9768456445 chr1,245362206,245362406,HSE514,20.8510241208 chr1,245748622,245748822,HSE400,15.8809014188 chr1,245751245,245751445,HSE614,11.9977672181 chr10,1796728,1796928,HSE887,8.8759918247 chr10,2188344,2188544,HSE210,17.5664225697 chr10,2435771,2435971,HSE331,14.4636659658 chr10,3441348,3441548,HSE787,8.9210657436 chr10,4012461,4012661,HSE959,9.8681000165 chr10,4659880,4660080,HSE109,39.294649348 chr10,5734710,5734910,HSE80,39.253086776 chr10,5918486,5918686,HSE902,13.4688692863 chr10,11575356,11575556,HSE741,13.0848348743 chr10,13005187,13005387,HSE721,10.6222160126 chr10,14195913,14196113,HSE544,13.1977478994 chr10,14227600,14227810,HSE569,12.7906533757 chr10,14364637,14364837,HSE882,8.5664694124 chr10,17825468,17825668,HSE300,17.0051022824 chr10,22748482,22748683,HSE737,14.6364554664 chr10,25811340,25811541,HSE58,31.2099871039 chr10,30602601,30602801,HSE23,49.0758646578 chr10,31080176,31080376,HSE918,9.2503197227 chr10,33270658,33270858,HSE949,11.4436365263 chr10,33294606,33294809,HSE995,12.672352369 chr10,38534930,38535130,HSE430,15.2638491526 chr10,44435726,44435926,HSE655,14.2956522267 chr10,44729078,44729278,HSE392,17.4461418303 chr10,46168148,46168348,HSE152,37.019176366 chr10,47640802,47641002,HSE244,20.7338411549 chr10,52710256,52710456,HSE489,16.0012334733 chr10,64400190,64400390,HSE143,23.5037143277 chr10,71081718,71081918,HSE723,10.7476600785 chr10,71470586,71470786,HSE37,54.1450393752 chr10,71536580,71536780,HSE664,12.2052671593 chr10,72471766,72471966,HSE783,17.3113965123 chr10,73599983,73600183,HSE403,21.5414372314 chr10,75126686,75126888,HSE71,29.3819259713 chr10,79473783,79473983,HSE43,45.6987326408 chr10,83728971,83729171,HSE212,18.9337565568 chr10,87807719,87807919,HSE859,8.6435515636 chr10,87872095,87872295,HSE481,12.756492704 chr10,88160082,88160282,HSE845,11.0856156511 chr10,88600426,88600626,HSE424,15.9686715881 chr10,90683407,90683607,HSE476,14.8937152174 chr10,90686708,90686908,HSE479,15.6187716761 chr10,93044375,93044575,HSE777,9.7964029914 chr10,94521429,94521629,HSE151,31.5694945963 chr10,97443603,97443803,HSE419,12.9499575441 chr10,97620593,97620793,HSE886,12.518441498 chr10,97667629,97667829,HSE201,34.3491300983 chr10,99223587,99223788,HSE446,23.975955174 chr10,104629190,104629462,HSE784,14.8155161441 chr10,105527665,105527865,HSE467,20.6230257395 chr10,106258325,106258525,HSE665,10.9578861725 chr10,107102583,107102783,HSE216,19.2077151804 chr10,108788448,108788648,HSE699,10.646721976 chr10,108792229,108792429,HSE432,17.1288876915 chr10,109186134,109186334,HSE960,11.8900697742 chr10,115258601,115258801,HSE108,28.6223256015 chr10,120523320,120523520,HSE531,21.3827794751 chr10,121452806,121453006,HSE989,8.0812359893 chr10,123179381,123179581,HSE732,9.5557622755 chr10,124108496,124108696,HSE927,8.9214956882 chr10,129573104,129573305,HSE54,44.0543064465 chr11,353431,353635,HSE551,15.321918756 chr11,3181559,3181759,HSE793,14.7046892301 chr11,6947739,6947942,HSE92,27.9827053442 chr11,13157034,13157234,HSE241,21.0632306147 chr11,15139410,15139611,HSE456,12.2189043207 chr11,15910292,15910492,HSE724,13.7294254547 chr11,16795802,16796002,HSE799,12.802186876 chr11,17550965,17551165,HSE639,14.1086468033 chr11,17943304,17943504,HSE891,11.4856542942 chr11,21656183,21656383,HSE700,14.9043957142 chr11,21662095,21662295,HSE440,17.2950629632 chr11,21673343,21673543,HSE894,8.2797517913 chr11,23447019,23447219,HSE6,75.8807514012 chr11,24085949,24086149,HSE492,15.300706048 chr11,24275024,24275224,HSE651,11.4443945585 chr11,25505910,25506110,HSE186,21.8909002434 chr11,31021450,31021650,HSE877,9.1198497163 chr11,31706283,31706483,HSE74,40.630635226 chr11,33868837,33869038,HSE857,11.3116139483 chr11,35595495,35595695,HSE183,19.9118859606 chr11,38009596,38009796,HSE907,9.9148121335 chr11,39564123,39564323,HSE936,9.1935010973 chr11,41468242,41468442,HSE36,44.9449175171 chr11,42922737,42922937,HSE200,32.2091247664 chr11,44407611,44407811,HSE657,10.3471228352 chr11,44747886,44748086,HSE475,15.1102404858 chr11,44986217,44986417,HSE619,10.668936584 chr11,45047769,45047969,HSE851,10.1383014015 chr11,45111276,45111476,HSE866,10.220733306 chr11,45369052,45369252,HSE627,12.2239177674 chr11,48016992,48017192,HSE767,10.1950143759 chr11,49411924,49412124,HSE512,18.574232402 chr11,63859735,63859935,HSE770,14.6837739902 chr11,63865941,63866141,HSE184,35.4114322006 chr11,65244823,65245023,HSE265,23.3441037512 chr11,67784892,67785092,HSE824,9.8386696158 chr11,73249610,73249812,HSE762,9.9839312996 chr11,73694258,73694459,HSE176,22.3483349613 chr11,76842280,76842482,HSE919,11.0703469831 chr11,78306590,78306790,HSE113,25.2078122498 chr11,79767266,79767466,HSE69,30.2148994861 chr11,81284367,81284567,HSE695,9.8185385502 chr11,92228348,92228548,HSE539,20.2344657972 chr11,100340586,100340786,HSE388,15.4919091437 chr11,104561099,104561299,HSE812,15.176333042 chr11,108070756,108070956,HSE809,9.4991448266 chr11,111156211,111156411,HSE714,10.0243534212 chr11,115147624,115147824,HSE134,28.6194026789 chr11,115846389,115846603,HSE722,16.8886385104 chr11,117592848,117593048,HSE705,13.7637540477 chr11,120106662,120106862,HSE937,10.9173422723 chr11,123178915,123179115,HSE672,11.3022531999 chr11,124816687,124816887,HSE601,14.6082914089 chr11,125805553,125805756,HSE945,20.5577441884 chr11,126357947,126358147,HSE493,15.3602555927 chr11,126976167,126976367,HSE968,9.1843932734 chr11,128052898,128053098,HSE911,12.5881181856 chr11,129137803,129138003,HSE311,18.8031509882 chr11,131335724,131335924,HSE517,23.7420612884 chr11,131510396,131510597,HSE406,27.563239098 chr11,131526809,131527010,HSE398,14.0540782683 chr11,132029024,132029225,HSE232,18.1785539558 chr11,132863261,132863461,HSE792,9.4066623256 chr12,1619367,1619567,HSE271,19.5724460831 chr12,2393824,2394024,HSE153,38.5409899219 chr12,4310999,4311199,HSE612,13.3477965884 chr12,4715838,4716038,HSE124,22.969040262 chr12,5977983,5978183,HSE981,8.9923072646 chr12,6649669,6649869,HSE666,18.8465977854 chr12,8983842,8984042,HSE206,21.6055505061 chr12,10253851,10254051,HSE850,13.368224383 chr12,12009176,12009379,HSE996,8.2025875972 chr12,14088837,14089037,HSE361,16.6852824277 chr12,15118302,15118502,HSE158,36.7485762947 chr12,15186700,15186902,HSE472,17.2685676671 chr12,15283754,15283954,HSE862,11.6244209976 chr12,16064197,16064397,HSE796,18.7455509923 chr12,23364365,23364565,HSE716,12.767223137 chr12,29608107,29608307,HSE84,29.4815974061 chr12,30672107,30672307,HSE459,16.6113321667 chr12,31079386,31079586,HSE928,15.5521683508 chr12,31233235,31233435,HSE817,14.0452336182 chr12,32029945,32030149,HSE3,134.4896822737 chr12,32418004,32418204,HSE443,14.9171957071 chr12,32715083,32715283,HSE566,11.7700863315 chr12,41442900,41443100,HSE448,15.8907964207 chr12,44547984,44548184,HSE595,19.5258881951 chr12,47299063,47299263,HSE710,17.9511083583 chr12,51318352,51318553,HSE278,38.5126987285 chr12,51714654,51714854,HSE105,36.1187300648 chr12,60334522,60334722,HSE296,18.4243312773 chr12,64388551,64388751,HSE411,16.4173206854 chr12,64960555,64960755,HSE893,11.4399085223 chr12,65100062,65100262,HSE677,12.0906755698 chr12,67180805,67181005,HSE302,18.0232362712 chr12,67820036,67820236,HSE693,28.0050105025 chr12,67824724,67824924,HSE562,19.9790499282 chr12,70590594,70590798,HSE938,10.4065399522 chr12,72665258,72665458,HSE870,9.4908568562 chr12,72843735,72843935,HSE352,14.6912721831 chr12,72903763,72903963,HSE622,12.4776159762 chr12,76258547,76258747,HSE366,15.4992304899 chr12,81139497,81139697,HSE263,19.1724365769 chr12,83358298,83358498,HSE956,10.9748220911 chr12,88826891,88827091,HSE470,14.4491790975 chr12,93693111,93693311,HSE337,17.5773205057 chr12,93793026,93793226,HSE231,36.7866454534 chr12,93964730,93964930,HSE701,14.9318726929 chr12,94680437,94680638,HSE800,10.1294106105 chr12,95448207,95448407,HSE85,41.328100082 chr12,98833530,98833731,HSE572,21.9773288843 chr12,102047388,102047588,HSE177,36.6536035992 chr12,105121890,105122090,HSE733,12.9059778339 chr12,106581316,106581516,HSE974,11.9119885347 chr12,108780634,108780834,HSE195,21.1967998006 chr12,110145174,110145374,HSE326,19.9633772792 chr12,110147281,110147481,HSE269,19.4570397688 chr12,116307853,116308063,HSE340,22.419418574 chr12,117846190,117846392,HSE393,15.5403001234 chr12,117850284,117850484,HSE557,16.5566891443 chr12,123543660,123543860,HSE861,10.4781563732 chr12,128314695,128314895,HSE528,13.3821736799 chr12,128455126,128455326,HSE906,9.3701763362 chr12,131041357,131041557,HSE827,8.9137558728 chr12,131261218,131261419,HSE39,40.7349907933 chr12,131596799,131596999,HSE61,38.7314139499 chr12,131622505,131622705,HSE735,9.6402688286 chr12,133175461,133175661,HSE560,17.0748830696 chr13,21347729,21347929,HSE450,18.700409108 chr13,24148647,24148847,HSE923,12.1565857536 chr13,29459319,29459519,HSE4,83.6939521234 chr13,29464970,29465170,HSE235,21.5042630726 chr13,33536518,33536718,HSE484,11.9616526488 chr13,36045055,36045255,HSE466,15.1339680818 chr13,37089667,37089867,HSE53,34.1508321281 chr13,38195831,38196031,HSE63,29.4908349793 chr13,47090817,47091017,HSE240,20.9194834678 chr13,47169753,47169953,HSE795,10.6129192636 chr13,48685195,48685395,HSE383,17.4161328951 chr13,52195812,52196012,HSE804,33.9458114683 chr13,52928037,52928237,HSE971,10.2210243551 chr13,58387875,58388075,HSE898,9.7709738074 chr13,63964002,63964202,HSE155,21.8968073472 chr13,63982654,63982855,HSE755,10.6894623919 chr13,66918963,66919163,HSE942,8.137382847 chr13,78382044,78382244,HSE709,13.2425052878 chr13,79968085,79968285,HSE207,19.0520301367 chr13,81969573,81969773,HSE464,17.1316537183 chr13,88325009,88325225,HSE169,22.404724465 chr13,94551722,94551922,HSE47,39.9300964523 chr13,96720972,96721172,HSE242,17.6289298545 chr13,99193496,99193696,HSE775,11.8286452332 chr13,102857806,102858006,HSE175,26.1349566103 chr13,103659347,103659547,HSE879,9.4672756235 chr13,106860061,106860261,HSE523,15.2219187287 chr13,107025740,107025940,HSE545,22.9167462321 chr13,107144094,107144294,HSE826,10.0932827937 chr13,111223922,111224122,HSE55,37.6150776113 chr14,21439340,21439545,HSE697,14.9749639521 chr14,24505425,24505632,HSE598,21.3172605404 chr14,25814644,25814844,HSE884,8.4198607932 chr14,26543395,26543598,HSE239,19.7243319678 chr14,31281660,31281860,HSE64,42.0539122341 chr14,33531812,33532012,HSE374,13.6641312978 chr14,59129293,59129493,HSE803,12.357054467 chr14,59386085,59386285,HSE932,9.6556661977 chr14,61083031,61083231,HSE780,12.1130184536 chr14,65539073,65539273,HSE324,18.8187496116 chr14,76804206,76804406,HSE129,28.6611225834 chr14,81636859,81637059,HSE65,73.8674420954 chr14,81790696,81790896,HSE209,42.4669581462 chr14,91091974,91092174,HSE480,17.813206233 chr14,91716580,91716780,HSE469,17.2797598596 chr14,93949158,93949358,HSE746,9.1621125053 chr14,95071723,95071923,HSE924,8.9302006066 chr14,102783279,102783479,HSE816,24.4849615987 chr14,105147964,105148164,HSE939,11.1238681848 chr15,27524028,27524228,HSE431,15.0234209239 chr15,28817788,28817988,HSE191,21.3327480149 chr15,29247425,29247625,HSE279,18.2157833949 chr15,30182592,30182792,HSE840,10.1016943223 chr15,31792516,31792716,HSE429,15.2456002355 chr15,33752266,33752466,HSE806,7.7973232785 chr15,33958947,33959147,HSE650,11.2642323912 chr15,39474262,39474462,HSE703,12.786143498 chr15,52554008,52554208,HSE832,10.0325076389 chr15,54967526,54967726,HSE230,24.1378839612 chr15,55510355,55510555,HSE339,18.1931930527 chr15,61269359,61269560,HSE1,89.2659435972 chr15,61979890,61980090,HSE922,8.1022018494 chr15,62752114,62752314,HSE611,14.5613676786 chr15,62917755,62917955,HSE305,28.6662298913 chr15,68723591,68723791,HSE462,23.2304619822 chr15,69830362,69830562,HSE757,10.8328824475 chr15,72203345,72203545,HSE147,31.2986371967 chr15,74076783,74076983,HSE771,12.0826546194 chr15,74596170,74596370,HSE993,12.6758156277 chr15,74641836,74642036,HSE221,25.8164928998 chr15,75523071,75523271,HSE874,9.5089830845 chr15,80478573,80478773,HSE211,20.487080964 chr15,80828906,80829107,HSE277,17.6534731911 chr15,81616460,81616661,HSE101,37.8792330626 chr15,81861640,81861840,HSE987,12.411938064 chr15,81868202,81868403,HSE587,14.0269438152 chr15,82201705,82201905,HSE376,27.8796005059 chr15,86374626,86374826,HSE588,14.9950787059 chr15,88418444,88418645,HSE580,12.4183759864 chr15,88670015,88670215,HSE521,11.1843930949 chr15,92692436,92692637,HSE26,72.0593711439 chr15,98775365,98775565,HSE381,14.9440294085 chr15,102501629,102501829,HSE592,17.8647492194 chr16,626697,626897,HSE208,23.3415017382 chr16,970811,971011,HSE408,18.879805744 chr16,3137379,3137579,HSE656,13.1191220261 chr16,11114848,11115048,HSE998,11.8718870193 chr16,11173789,11173989,HSE128,39.6118207734 chr16,19756698,19756898,HSE349,18.1890890859 chr16,22776292,22776492,HSE631,10.2281468082 chr16,23037379,23037579,HSE661,11.5338091882 chr16,24508521,24508721,HSE731,10.9292976321 chr16,27323049,27323272,HSE62,34.6319360162 chr16,30204374,30204574,HSE426,34.9445834473 chr16,31885117,31885317,HSE805,17.0726534038 chr16,48565689,48565889,HSE914,14.4461848582 chr16,52484957,52485157,HSE999,9.9367824367 chr16,56581581,56581781,HSE386,14.3620121795 chr16,59736519,59736719,HSE342,19.0406902327 chr16,66545956,66546156,HSE905,9.2931748668 chr16,68484705,68484905,HSE218,20.7512770405 chr16,68507748,68507957,HSE548,18.0413570871 chr16,73322142,73322343,HSE702,12.5680470461 chr16,78355884,78356084,HSE303,20.3741349926 chr16,89307265,89307465,HSE18,70.9382862202 chr16,89679724,89679939,HSE578,17.1619789211 chr16,89988292,89988493,HSE347,20.0128724894 chr17,999331,999532,HSE482,15.5034626446 chr17,5579439,5579642,HSE48,43.9178228518 chr17,6111243,6111443,HSE198,30.4698391934 chr17,8279924,8280124,HSE45,65.9896646322 chr17,8759325,8759525,HSE422,15.0545562099 chr17,9340019,9340219,HSE948,9.8219471135 chr17,11166409,11166610,HSE122,22.963059202 chr17,12830908,12831108,HSE145,27.0976894901 chr17,13298874,13299074,HSE156,34.5395597863 chr17,17002215,17002415,HSE654,16.2936251743 chr17,17302370,17302570,HSE372,29.9282249338 chr17,21030152,21030352,HSE903,14.116644535 chr17,21367528,21367728,HSE833,9.7516907593 chr17,30088186,30088386,HSE678,13.3589311568 chr17,33774498,33774698,HSE818,9.4607428819 chr17,34541456,34541656,HSE553,11.9696962671 chr17,42385237,42385437,HSE149,30.3729032338 chr17,43419034,43419235,HSE437,27.2450803684 chr17,45681996,45682196,HSE438,15.031012053 chr17,49008988,49009188,HSE538,15.8789561003 chr17,49433023,49433224,HSE11,58.5889197596 chr17,49687897,49688097,HSE413,16.1529684506 chr17,49807832,49808032,HSE2,84.3204244342 chr17,50624462,50624662,HSE20,42.9975194309 chr17,50767466,50767666,HSE258,24.1676282047 chr17,53412056,53412256,HSE243,26.149501739 chr17,53828393,53828601,HSE617,14.5154098498 chr17,55785272,55785472,HSE892,10.3430074886 chr17,57998741,57998941,HSE978,10.1861279752 chr17,59327373,59327573,HSE774,9.3428992552 chr17,59589126,59589326,HSE529,18.5934605421 chr17,62254441,62254641,HSE133,33.7747197413 chr17,64370576,64370776,HSE964,7.825535696 chr17,72113540,72113740,HSE607,21.5911019025 chr17,72967433,72967633,HSE377,16.2493907656 chr17,73966346,73966547,HSE881,9.5338664732 chr17,75125788,75125988,HSE407,21.4349393235 chr17,75279445,75279646,HSE527,11.5655867334 chr17,79050003,79050203,HSE445,15.299617834 chr17,80189664,80189864,HSE957,8.575676987 chr18,2847905,2848105,HSE680,16.1823388257 chr18,3297314,3297514,HSE51,55.4969408846 chr18,4107882,4108082,HSE756,10.6509843928 chr18,4455211,4455413,HSE173,26.658560462 chr18,5960317,5960517,HSE404,17.5337872123 chr18,6610590,6610790,HSE5,67.4765594962 chr18,6878970,6879170,HSE21,114.4591803017 chr18,6941554,6941754,HSE925,8.8831899962 chr18,8067140,8067340,HSE515,15.8060479397 chr18,8982773,8982973,HSE674,10.8336659886 chr18,8991089,8991289,HSE871,9.2212572719 chr18,11092726,11092926,HSE603,14.5685707705 chr18,20949254,20949454,HSE940,10.1089249342 chr18,22006557,22006757,HSE575,21.5810391792 chr18,24341571,24341772,HSE865,12.220860758 chr18,30186665,30186865,HSE96,29.1942230497 chr18,33597390,33597591,HSE935,14.2979081707 chr18,38116709,38116909,HSE94,27.5166517092 chr18,38614509,38614709,HSE516,22.9528532124 chr18,41456364,41456564,HSE483,22.2977384345 chr18,43802028,43802228,HSE508,12.8490635247 chr18,46386532,46386733,HSE676,14.0371357841 chr18,47441756,47441956,HSE282,17.402175324 chr18,48344045,48344245,HSE640,26.5533837237 chr18,57865414,57865614,HSE637,12.1799986999 chr18,60810573,60810773,HSE712,10.7941794086 chr18,66106367,66106567,HSE78,34.0539230971 chr18,67067626,67067826,HSE748,20.3756153543 chr18,67206189,67206389,HSE707,14.8194102675 chr18,77862401,77862601,HSE778,8.9773592552 chr19,1855466,1855666,HSE615,21.0205633353 chr19,7864445,7864654,HSE420,22.2334775436 chr19,7936518,7936718,HSE1000,9.890269251 chr19,14134468,14134668,HSE552,19.247739132 chr19,16568459,16568659,HSE690,12.8248560895 chr19,22053766,22053966,HSE325,15.1569835215 chr19,28284760,28284962,HSE35,44.2571129827 chr19,28609278,28609478,HSE262,25.334253518 chr19,28660492,28660692,HSE644,23.3221045263 chr19,30811887,30812087,HSE120,27.7702458019 chr19,35800455,35800659,HSE498,13.5519561083 chr19,36792015,36792215,HSE953,7.5220582555 chr19,39122462,39122662,HSE582,17.8131880246 chr19,39167150,39167350,HSE395,15.4118441749 chr19,42905988,42906189,HSE934,12.7825653114 chr19,43059635,43059838,HSE535,11.755421727 chr19,44711537,44711737,HSE798,18.193542685 chr19,50175593,50175793,HSE781,13.7950661987 chr19,51289242,51289442,HSE917,9.3858171773 chr19,51900838,51901038,HSE453,18.6017463436 chr19,53039209,53039409,HSE463,19.5962420571 chr2,4564065,4564265,HSE689,11.0832062757 chr2,9136176,9136376,HSE166,25.108740724 chr2,11761490,11761690,HSE955,9.1404770745 chr2,14359777,14359977,HSE729,9.1222131108 chr2,15208167,15208367,HSE751,11.003441743 chr2,17699479,17699679,HSE759,12.4462128012 chr2,17721672,17721872,HSE742,14.1970571736 chr2,20024086,20024286,HSE83,28.7067393754 chr2,23725997,23726197,HSE449,24.6822465458 chr2,25752130,25752330,HSE346,16.6877757619 chr2,29434845,29435045,HSE841,9.2654187933 chr2,29840944,29841144,HSE785,11.1516309255 chr2,33308933,33309133,HSE810,10.6180361689 chr2,40274443,40274643,HSE543,16.5775325601 chr2,40277040,40277240,HSE901,17.7522146515 chr2,43020786,43020986,HSE536,13.7021711702 chr2,43525771,43525971,HSE140,23.6931498342 chr2,45907824,45908024,HSE790,12.332179446 chr2,49482822,49483023,HSE764,11.6109483119 chr2,49776850,49777050,HSE382,20.4425087695 chr2,49829759,49829959,HSE559,13.8791198577 chr2,50199950,50200150,HSE306,21.1830037687 chr2,50522255,50522455,HSE294,20.4771502209 chr2,50573272,50573472,HSE520,11.3849059039 chr2,53710769,53710969,HSE673,11.4462899835 chr2,54846090,54846291,HSE652,12.3643734532 chr2,55381344,55381548,HSE333,16.5384641969 chr2,55649064,55649265,HSE161,26.4627203219 chr2,65290575,65290775,HSE860,14.2637154595 chr2,65615621,65615821,HSE811,10.640516431 chr2,68546802,68547002,HSE585,12.806795592 chr2,72975882,72976082,HSE506,11.8250162965 chr2,74234353,74234554,HSE447,19.3111885875 chr2,76692583,76692783,HSE308,24.5495576527 chr2,84914260,84914460,HSE273,18.7931730309 chr2,85289844,85290044,HSE490,17.6255300394 chr2,85378807,85379007,HSE442,18.4954416724 chr2,87051461,87051665,HSE139,28.4199186304 chr2,87064810,87065010,HSE708,10.458886264 chr2,87067122,87067449,HSE379,30.0280259634 chr2,87069310,87069510,HSE885,16.1725172352 chr2,88316314,88316515,HSE214,34.3179189629 chr2,88414573,88414773,HSE220,18.6297613359 chr2,89034794,89034994,HSE641,10.882267071 chr2,91750589,91750877,HSE327,20.2352669039 chr2,91762380,91762580,HSE468,12.6786942321 chr2,97019268,97019468,HSE786,8.3601047373 chr2,98758986,98759186,HSE679,16.1171102076 chr2,100697871,100698071,HSE706,13.479967802 chr2,100699949,100700149,HSE163,38.236433871 chr2,104094111,104094311,HSE59,33.1831421211 chr2,106095656,106095858,HSE390,15.6220351252 chr2,106554244,106554444,HSE497,18.2333367245 chr2,109968022,109968222,HSE763,10.4860988331 chr2,110515113,110515315,HSE19,48.1747064465 chr2,110806206,110806406,HSE977,8.2999349047 chr2,111400651,111400851,HSE808,9.0427754883 chr2,112020360,112020560,HSE444,14.0773788267 chr2,112221956,112222156,HSE720,15.6782160603 chr2,114395544,114395744,HSE67,33.2899481068 chr2,119068189,119068389,HSE104,27.8409422337 chr2,119071047,119071247,HSE25,58.9652879287 chr2,121088717,121088917,HSE726,14.2115751447 chr2,122336997,122337197,HSE249,20.8047512788 chr2,124279519,124279719,HSE495,11.9685617057 chr2,124730178,124730378,HSE30,48.1797064921 chr2,131094775,131094975,HSE988,12.5951506631 chr2,131484996,131485196,HSE76,33.2819734412 chr2,133300942,133301142,HSE997,7.8669044953 chr2,134090771,134090971,HSE180,24.5244184665 chr2,137008268,137008468,HSE307,16.6788740279 chr2,137015528,137015728,HSE947,7.5774875118 chr2,142934541,142934741,HSE345,21.8622427277 chr2,150036853,150037053,HSE807,12.9999546517 chr2,165778793,165778993,HSE358,17.0239953486 chr2,177391858,177392058,HSE260,19.1403997668 chr2,182327537,182327737,HSE681,17.5160573028 chr2,182393251,182393451,HSE40,38.4626716079 chr2,194117851,194118051,HSE321,18.0633419546 chr2,202563650,202563850,HSE626,12.3289416007 chr2,202741843,202742043,HSE359,20.9626551138 chr2,207294294,207294494,HSE670,11.1162813392 chr2,210363875,210364075,HSE734,12.3968615154 chr2,216306316,216306516,HSE868,14.1928417965 chr2,217522315,217522515,HSE293,35.0627763697 chr2,218641534,218641734,HSE82,37.7257060365 chr2,225243534,225243734,HSE416,20.099420911 chr2,229706577,229706777,HSE943,10.1343101996 chr2,231797519,231797723,HSE719,12.2696761634 chr2,232478862,232479063,HSE856,8.5861916221 chr2,236011384,236011584,HSE496,11.7277379839 chr2,238512362,238512563,HSE608,13.5849351928 chr2,240078429,240078629,HSE174,24.7169603423 chr2,241308943,241309143,HSE590,12.5097816417 chr2,241406758,241406958,HSE727,15.8620577497 chr20,1791874,1792074,HSE487,14.0309392186 chr20,1864025,1864225,HSE950,9.0977150918 chr20,1871245,1871445,HSE16,56.7490572952 chr20,2012791,2012991,HSE87,31.037092624 chr20,3629754,3629958,HSE215,20.9130441286 chr20,3660554,3660754,HSE193,24.974343769 chr20,4063903,4064104,HSE111,56.1553976804 chr20,14705232,14705432,HSE33,47.5410662718 chr20,16719908,16720108,HSE270,16.3543605271 chr20,32774062,32774262,HSE309,18.1246253801 chr20,34463418,34463619,HSE367,15.2757490918 chr20,34681486,34681692,HSE753,9.3728031841 chr20,34961484,34961684,HSE355,27.5542266393 chr20,36875508,36875708,HSE12,88.6429013544 chr20,39492043,39492243,HSE616,13.836833653 chr20,39504403,39504603,HSE791,11.0391746143 chr20,39522366,39522566,HSE522,15.4476576681 chr20,41278899,41279099,HSE130,24.1052112576 chr20,41281047,41281248,HSE138,25.1054094538 chr20,43678567,43678767,HSE118,27.5802502403 chr20,47354941,47355141,HSE829,10.0084428 chr20,48196544,48196744,HSE13,62.2017999936 chr20,48391843,48392070,HSE343,17.2886280147 chr20,48451672,48451872,HSE658,15.2435487162 chr20,57999712,57999912,HSE593,11.851459255 chr20,58010717,58010917,HSE888,8.4178547638 chr20,58028998,58029198,HSE589,11.8594187455 chr20,62892549,62892749,HSE22,49.3467939523 chr21,18183390,18183590,HSE846,12.0921264014 chr21,25470304,25470504,HSE848,9.043990116 chr21,29314093,29314293,HSE540,11.1333587451 chr21,29522195,29522395,HSE90,42.5669364456 chr21,30279211,30279411,HSE802,8.9817364292 chr21,31599287,31599487,HSE255,25.8534564105 chr21,34305184,34305384,HSE75,34.8701267573 chr21,40357115,40357316,HSE931,14.2521728213 chr21,40369917,40370117,HSE596,13.6512982833 chr21,42458559,42458759,HSE941,7.986093663 chr21,42461230,42461430,HSE46,32.6233218231 chr21,45610526,45610726,HSE373,19.1339980285 chr22,18647319,18647519,HSE600,15.4170005372 chr22,21387111,21387311,HSE465,16.7936897957 chr22,25800344,25800546,HSE855,15.133649847 chr22,27530972,27531172,HSE91,32.8296818218 chr22,27613121,27613321,HSE878,9.9026827262 chr22,30938555,30938755,HSE991,12.3953908195 chr22,37608742,37608942,HSE199,25.5079108823 chr22,38213774,38213975,HSE336,29.8698643259 chr22,38875187,38875387,HSE31,75.0968010157 chr22,45034999,45035200,HSE944,11.0058314418 chr22,47560535,47560735,HSE820,9.6159416626 chr22,49263785,49263985,HSE123,27.4756435262 chr3,1033708,1033908,HSE547,11.8167271684 chr3,4648618,4648819,HSE594,13.1354478948 chr3,6170925,6171125,HSE274,19.6422205175 chr3,9861617,9861817,HSE561,16.1351899583 chr3,20581162,20581362,HSE88,84.6550501758 chr3,22766006,22766206,HSE926,7.4746803691 chr3,25039886,25040088,HSE745,12.5677664568 chr3,32452491,32452691,HSE958,8.8781271039 chr3,34337067,34337267,HSE238,23.4517631822 chr3,44106796,44106996,HSE645,14.6078435944 chr3,45454944,45455144,HSE213,19.0172183728 chr3,50468482,50468682,HSE70,32.3982070336 chr3,50553584,50553784,HSE409,31.7674822371 chr3,50759518,50759718,HSE556,12.7286106999 chr3,51312927,51313129,HSE179,22.7291813917 chr3,54024155,54024359,HSE606,12.4118581011 chr3,55583333,55583544,HSE895,20.4730234134 chr3,57432272,57432472,HSE170,23.9015532829 chr3,57731978,57732178,HSE979,13.0317416952 chr3,58143711,58143912,HSE488,21.4279391783 chr3,59117232,59117432,HSE310,21.6692936097 chr3,59537533,59537733,HSE831,10.4335147498 chr3,60313490,60313690,HSE797,11.4508642018 chr3,60739661,60739861,HSE222,37.1649368806 chr3,61899965,61900165,HSE972,9.9582764633 chr3,62859461,62859661,HSE671,12.0850192866 chr3,62874394,62874594,HSE454,12.2791253714 chr3,63748080,63748280,HSE387,14.5767368616 chr3,74103231,74103431,HSE633,15.5075880796 chr3,85652655,85652855,HSE203,18.8184809601 chr3,97829189,97829389,HSE776,9.1102578423 chr3,103166013,103166213,HSE896,9.9523912683 chr3,105602785,105602985,HSE433,40.5785957197 chr3,107148262,107148462,HSE261,18.4043131499 chr3,109288204,109288404,HSE356,14.9310679943 chr3,111197315,111197518,HSE370,15.859503117 chr3,112219616,112219816,HSE368,15.0704271372 chr3,123405019,123405220,HSE115,27.2314354098 chr3,124880427,124880628,HSE579,14.8608441809 chr3,127740739,127740949,HSE314,26.0837216172 chr3,128256027,128256227,HSE630,13.9854481485 chr3,132829089,132829289,HSE474,15.9443427668 chr3,133123857,133124057,HSE471,13.3464634396 chr3,136537878,136538084,HSE505,14.6728277902 chr3,136915437,136915640,HSE584,13.4830219375 chr3,136922639,136922839,HSE304,16.1133399857 chr3,143048781,143048981,HSE663,12.6197529914 chr3,145155998,145156198,HSE963,7.937403129 chr3,150102651,150102851,HSE441,27.9319328699 chr3,152314041,152314241,HSE863,9.389594265 chr3,152446287,152446487,HSE202,21.6487393195 chr3,152497432,152497632,HSE782,15.2232552726 chr3,153501047,153501247,HSE973,8.9058148252 chr3,156106412,156106612,HSE298,17.4725292847 chr3,156467111,156467311,HSE984,12.8714197176 chr3,159583162,159583362,HSE245,24.3670741479 chr3,167647788,167647988,HSE162,23.142026511 chr3,167754605,167754805,HSE157,25.5019260862 chr3,168309609,168309809,HSE985,8.3878224111 chr3,170459966,170460166,HSE299,23.2572944826 chr3,171083365,171083565,HSE329,19.6170760846 chr3,171622769,171622969,HSE141,35.5269780554 chr3,173303292,173303492,HSE635,12.8762248581 chr3,173340053,173340253,HSE910,13.8353115715 chr3,175722586,175722786,HSE154,26.1510590855 chr3,177471354,177471554,HSE332,24.5244184665 chr3,184084866,184085066,HSE428,17.9640764471 chr3,192869895,192870095,HSE246,22.5875292012 chr3,194592300,194592501,HSE362,36.8838467218 chr3,196346498,196346699,HSE280,19.4679975855 chr3,197199171,197199371,HSE876,13.6753444396 chr4,1209278,1209519,HSE564,13.9004104602 chr4,1682431,1682632,HSE233,22.8144061287 chr4,2673293,2673493,HSE435,15.7039657822 chr4,5205616,5205843,HSE875,9.1366703212 chr4,5774976,5775176,HSE248,21.1824928836 chr4,7153027,7153227,HSE983,9.7283332501 chr4,7158073,7158273,HSE164,31.8768175016 chr4,7987860,7988060,HSE685,14.8308227861 chr4,8317712,8317912,HSE912,11.2750910314 chr4,10298688,10298888,HSE7,56.3001544011 chr4,10307533,10307733,HSE478,12.8133414228 chr4,10410626,10410826,HSE197,21.3709305662 chr4,16325714,16325914,HSE351,19.7714522278 chr4,16843354,16843554,HSE872,11.1479071197 chr4,17189915,17190115,HSE794,12.4374877889 chr4,17399671,17399871,HSE698,12.9259482624 chr4,18421836,18422036,HSE418,18.6650288814 chr4,20654001,20654201,HSE842,9.8868805638 chr4,35935735,35935935,HSE864,11.6965490821 chr4,37141687,37141887,HSE384,14.5996824189 chr4,37189108,37189308,HSE188,35.9761823699 chr4,38192503,38192704,HSE513,20.6670228424 chr4,41059770,41059970,HSE550,14.2940364222 chr4,41146900,41147100,HSE920,10.9173925032 chr4,42598985,42599185,HSE834,12.2213134666 chr4,43551753,43551953,HSE613,10.9664468122 chr4,52918093,52918293,HSE813,10.8199181309 chr4,67296659,67296859,HSE330,17.1184863176 chr4,68783414,68783614,HSE194,27.8865779207 chr4,73531199,73531399,HSE485,16.0641880211 chr4,79442149,79442349,HSE254,31.3053467034 chr4,96027806,96028006,HSE60,38.0420908281 chr4,98417455,98417655,HSE773,12.7094671878 chr4,102826820,102827020,HSE954,9.8953082642 chr4,108956348,108956548,HSE196,28.1854857987 chr4,109035842,109036042,HSE574,15.4815826792 chr4,109331833,109332033,HSE880,8.5800439974 chr4,112855557,112855758,HSE873,8.9177752517 chr4,113045048,113045248,HSE718,12.9338359083 chr4,115673675,115673875,HSE908,9.0213984225 chr4,126033815,126034015,HSE266,27.8144026005 chr4,126926133,126926333,HSE624,13.8939469933 chr4,134018135,134018335,HSE591,13.1426859966 chr4,135498284,135498484,HSE289,16.9047451361 chr4,139144837,139145037,HSE858,11.0683425726 chr4,142822079,142822279,HSE291,16.8719939446 chr4,143883214,143883420,HSE125,23.7564028153 chr4,155022297,155022497,HSE486,24.4008504204 chr4,155876015,155876219,HSE567,14.3792053309 chr4,156523530,156523730,HSE335,17.9101352191 chr4,163926681,163926881,HSE583,13.7123935 chr4,165404029,165404229,HSE965,10.3169505717 chr4,171168842,171169042,HSE500,12.3708601884 chr4,176356648,176356848,HSE586,12.5175850781 chr4,181756449,181756649,HSE116,25.0123586931 chr4,185517517,185517717,HSE768,10.7243756024 chr4,186037200,186037400,HSE849,9.15728802 chr4,187112549,187112749,HSE73,29.6147089784 chr4,188809131,188809331,HSE127,26.3736706202 chr5,9015815,9016015,HSE754,10.9860464939 chr5,9140785,9140986,HSE34,39.8342153912 chr5,9284766,9284966,HSE159,44.8345695381 chr5,9423793,9423993,HSE397,23.9014518508 chr5,9544694,9544894,HSE976,18.4787378901 chr5,13572514,13572715,HSE970,7.981996643 chr5,14844977,14845178,HSE623,11.8533435499 chr5,26843843,26844043,HSE609,10.9929164023 chr5,35374800,35375000,HSE992,11.2155834064 chr5,36059728,36059928,HSE743,9.7015183684 chr5,38011944,38012144,HSE182,23.9400859933 chr5,39421419,39421619,HSE825,14.5866459668 chr5,39533959,39534159,HSE760,9.9360929783 chr5,43066506,43066706,HSE288,64.294192312 chr5,50917138,50917340,HSE313,22.7435698695 chr5,51008092,51008292,HSE766,10.2876577874 chr5,58130163,58130363,HSE686,14.7084178919 chr5,68296916,68297116,HSE57,43.9761647554 chr5,70529394,70529594,HSE292,16.3167607327 chr5,73557824,73558024,HSE283,26.1537486394 chr5,81951100,81951300,HSE838,11.1124928906 chr5,82305319,82305520,HSE256,21.9987593589 chr5,93459233,93459433,HSE79,88.077239222 chr5,96180256,96180457,HSE112,28.97133338 chr5,98859253,98859453,HSE457,16.1598314579 chr5,102729064,102729264,HSE89,35.4121997783 chr5,107225179,107225379,HSE752,12.8723311418 chr5,108006706,108006906,HSE167,20.5736395644 chr5,112562162,112562362,HSE146,28.4736858728 chr5,115731439,115731639,HSE662,14.441740722 chr5,115870785,115870985,HSE889,11.1682472441 chr5,121403180,121403380,HSE738,10.4404525204 chr5,123709637,123709837,HSE385,34.5371219842 chr5,124534387,124534587,HSE189,41.4113583783 chr5,126015956,126016156,HSE350,48.9260098909 chr5,130651056,130651256,HSE353,19.9406119279 chr5,131054387,131054588,HSE275,16.8672736168 chr5,138872592,138872792,HSE97,33.7294648775 chr5,139113346,139113546,HSE499,24.5461507056 chr5,139503491,139503691,HSE135,22.5832471238 chr5,140365894,140366094,HSE477,22.7804027056 chr5,152496425,152496625,HSE297,23.9517433229 chr5,153157431,153157632,HSE994,7.2712465887 chr5,154985156,154985356,HSE27,47.3698229496 chr5,156798061,156798261,HSE421,18.0953061141 chr5,159614141,159614341,HSE504,16.644367563 chr5,169678151,169678351,HSE205,23.5872306039 chr5,169969996,169970196,HSE252,26.7061548422 chr5,170556363,170556585,HSE81,53.3489093817 chr5,170880119,170880319,HSE533,21.0213665516 chr5,176794222,176794423,HSE599,23.2425155735 chr5,177712082,177712283,HSE921,9.6878527385 chr6,1720956,1721156,HSE961,13.9546110024 chr6,2908962,2909162,HSE900,9.4385437272 chr6,3624882,3625082,HSE49,38.6547390654 chr6,6477697,6477897,HSE425,14.0308308131 chr6,6927996,6928198,HSE573,13.2644125542 chr6,7261536,7261736,HSE899,20.57594766 chr6,8857485,8857685,HSE226,19.7424552164 chr6,11764188,11764388,HSE423,13.6453117051 chr6,12657164,12657364,HSE541,24.5587040398 chr6,13069178,13069378,HSE148,25.4358868167 chr6,13071666,13071866,HSE328,21.7306804565 chr6,13440803,13441003,HSE854,11.7878221637 chr6,13605303,13605503,HSE439,16.0336294076 chr6,15357986,15358186,HSE772,12.1002596677 chr6,15953729,15953929,HSE223,22.2692616975 chr6,20879847,20880047,HSE789,11.3511396601 chr6,22074016,22074216,HSE830,17.8828299952 chr6,22223835,22224035,HSE847,11.3189534245 chr6,22754318,22754518,HSE765,10.933963726 chr6,24191674,24191874,HSE17,64.4494432065 chr6,24355724,24355924,HSE822,12.2330307591 chr6,24684045,24684245,HSE68,40.6121039818 chr6,24908692,24908892,HSE52,36.3357402285 chr6,24981608,24981808,HSE460,14.7455299562 chr6,26866614,26866814,HSE316,17.4817827982 chr6,26987817,26988017,HSE301,17.0996119934 chr6,28953271,28953471,HSE532,18.2243327433 chr6,30163047,30163247,HSE668,9.9208283821 chr6,32862428,32862628,HSE883,10.9304672886 chr6,33048550,33048750,HSE100,28.3569655356 chr6,37190063,37190263,HSE225,25.1598854782 chr6,37752532,37752732,HSE190,21.4887428904 chr6,37839872,37840072,HSE692,15.9827010242 chr6,42104428,42104628,HSE107,67.5217188687 chr6,42125896,42126096,HSE836,11.8802206566 chr6,48152098,48152298,HSE317,16.53498743 chr6,48440896,48441096,HSE410,20.2304692131 chr6,48531624,48531824,HSE14,56.1553976804 chr6,48595126,48595327,HSE8,86.3398854446 chr6,57207153,57207353,HSE779,15.3463418546 chr6,58265076,58265285,HSE632,13.3681109557 chr6,68599806,68600006,HSE526,12.8237395122 chr6,71584109,71584310,HSE114,27.0742020553 chr6,77915769,77915969,HSE236,16.9378739354 chr6,87169783,87169984,HSE546,11.573392587 chr6,89894075,89894277,HSE647,13.1942619616 chr6,90904900,90905100,HSE491,25.8304194594 chr6,92350596,92350796,HSE653,17.364572389 chr6,101840815,101841015,HSE131,28.2436702832 chr6,105240470,105240670,HSE604,18.8779663264 chr6,107221861,107222061,HSE844,13.1640641167 chr6,107611759,107611959,HSE904,13.5023269104 chr6,112619369,112619569,HSE369,17.0366032561 chr6,114999140,114999341,HSE821,10.3959314865 chr6,116138273,116138476,HSE730,13.0350910622 chr6,116621676,116621877,HSE929,9.2537035432 chr6,131428060,131428260,HSE284,18.8889226922 chr6,132596060,132596260,HSE990,8.2742348935 chr6,138104942,138105142,HSE253,23.0468563465 chr6,140757614,140757815,HSE967,10.1788738588 chr6,143968529,143968729,HSE852,8.5873299746 chr6,147322278,147322478,HSE268,19.7936739006 chr6,151695336,151695536,HSE519,19.0160524829 chr6,157265666,157265866,HSE119,24.7735526841 chr6,161392326,161392526,HSE334,18.7149756358 chr6,161740337,161740537,HSE713,9.6675137739 chr6,162838242,162838442,HSE132,26.1781329147 chr6,166188920,166189120,HSE417,14.5239466546 chr6,167892584,167892784,HSE814,8.7865263368 chr6,169077927,169078127,HSE110,27.1403888015 chr6,170794092,170794292,HSE524,13.6109134035 chr7,4198833,4199033,HSE801,11.1292518601 chr7,4949917,4950117,HSE172,21.4927855963 chr7,13461438,13461638,HSE228,17.5121934963 chr7,22122623,22122823,HSE930,12.3048991783 chr7,23062178,23062378,HSE509,25.1202244572 chr7,23246200,23246400,HSE257,22.9981606665 chr7,25559731,25559931,HSE966,8.5262161829 chr7,36664999,36665199,HSE605,16.0994530522 chr7,37372863,37373063,HSE204,19.2684485089 chr7,38492364,38492564,HSE597,12.8932215725 chr7,40616066,40616266,HSE380,17.182635963 chr7,47064486,47064686,HSE577,13.0570408987 chr7,47084856,47085056,HSE576,16.6838216652 chr7,48031471,48031671,HSE740,11.7452011534 chr7,48748205,48748405,HSE98,30.5044348782 chr7,66050120,66050320,HSE276,21.6755649273 chr7,66213153,66213353,HSE534,13.7182502443 chr7,66223024,66223224,HSE364,16.185690078 chr7,74867660,74867860,HSE618,16.9538639948 chr7,77015807,77016008,HSE415,18.6613812869 chr7,78390011,78390211,HSE602,16.2431492916 chr7,100434184,100434384,HSE869,13.0394840372 chr7,101616503,101616703,HSE951,9.2324134965 chr7,102153402,102153602,HSE975,9.0245761817 chr7,110397521,110397721,HSE916,12.3054838817 chr7,110400563,110400763,HSE338,16.3230063175 chr7,116607477,116607678,HSE621,18.3522646727 chr7,118072236,118072438,HSE694,11.1158534833 chr7,125587404,125587604,HSE455,17.556648341 chr7,145100201,145100414,HSE401,15.6280338065 chr7,145839272,145839472,HSE171,20.1680033829 chr7,145844066,145844266,HSE9,54.1909479525 chr7,147449071,147449271,HSE704,10.2463146144 chr7,148178409,148178609,HSE835,10.6172870294 chr7,151235436,151235636,HSE365,18.0341069228 chr8,2377506,2377706,HSE181,20.7659351902 chr8,2511126,2511326,HSE320,15.27069181 chr8,7266669,7266869,HSE819,8.435349452 chr8,13356648,13356848,HSE281,18.4464980795 chr8,14336890,14337104,HSE66,46.656249178 chr8,23032626,23032826,HSE412,26.2529950816 chr8,26601555,26601755,HSE890,11.220125934 chr8,26628311,26628511,HSE788,10.9551178433 chr8,28009163,28009363,HSE510,15.0235522925 chr8,30385906,30386106,HSE629,17.1134130395 chr8,34312488,34312688,HSE178,30.2724339491 chr8,49930877,49931077,HSE507,18.1489727778 chr8,50149648,50149848,HSE518,13.7760589052 chr8,52875070,52875270,HSE501,15.5123958666 chr8,53660034,53660234,HSE93,37.3464806395 chr8,54605619,54605820,HSE684,14.2519866729 chr8,62892804,62893004,HSE121,31.87775364 chr8,73254670,73254870,HSE185,23.4900882124 chr8,73412628,73412828,HSE38,37.3035911391 chr8,75862050,75862250,HSE620,13.858825618 chr8,88877867,88878069,HSE563,12.7713866408 chr8,92115315,92115515,HSE530,14.8449505644 chr8,92248603,92248804,HSE691,15.9124555229 chr8,93852146,93852346,HSE290,32.7317983798 chr8,95213282,95213482,HSE494,13.4997136969 chr8,98387996,98388197,HSE628,12.1439641326 chr8,105236101,105236301,HSE969,9.7479394792 chr8,115779521,115779721,HSE688,12.8255728825 chr8,122167781,122167981,HSE371,17.391031161 chr8,125610826,125611026,HSE165,35.3095256409 chr8,131652848,131653048,HSE363,19.456186422 chr8,134045654,134045854,HSE168,31.8082809938 chr8,134829150,134829350,HSE646,10.9003096576 chr8,144094801,144095001,HSE717,15.3147108573 chr8,144343458,144343659,HSE72,32.0323018157 chr8,145987786,145988059,HSE451,17.6753394576 chr9,7510288,7510488,HSE312,33.7426439062 chr9,10593086,10593286,HSE414,16.6788740279 chr9,11458448,11458648,HSE843,9.0784819552 chr9,12117105,12117305,HSE142,27.950209127 chr9,14993242,14993442,HSE634,14.4731722458 chr9,16301282,16301482,HSE102,50.9103305769 chr9,17765448,17765655,HSE739,18.2392565514 chr9,20825252,20825452,HSE946,12.4789871155 chr9,33402443,33402643,HSE555,16.1608716083 chr9,33523607,33523818,HSE224,19.2005783714 chr9,34170779,34170979,HSE341,24.6371580044 chr9,41824106,41824306,HSE769,10.5181109381 chr9,42019392,42019592,HSE28,41.4263636489 chr9,44245346,44245546,HSE32,37.5468627247 chr9,46661510,46661710,HSE378,18.5997971629 chr9,67872401,67872601,HSE272,19.7212214429 chr9,69651218,69651418,HSE15,56.5573849515 chr9,69654841,69655041,HSE50,50.1850231356 chr9,69723884,69724084,HSE126,32.6894006406 chr9,70077265,70077469,HSE357,19.5503409497 chr9,70090005,70090205,HSE56,47.863549167 chr9,72158059,72158265,HSE736,10.8002448051 chr9,72749929,72750129,HSE667,9.6151470352 chr9,81641307,81641507,HSE565,12.5343408216 chr9,85896509,85896709,HSE360,15.976780491 chr9,87992926,87993126,HSE682,10.0728691654 chr9,91288879,91289079,HSE570,20.1264215633 chr9,93232164,93232364,HSE267,16.2689597218 chr9,97610366,97610566,HSE571,14.362464282 chr9,97933380,97933580,HSE660,11.3259473431 chr9,100000625,100000826,HSE473,16.0316421967 chr9,100937326,100937526,HSE502,14.7363484454 chr9,112871691,112871891,HSE427,20.2200008236 chr9,116605468,116605668,HSE24,44.0402200911 chr9,118163144,118163344,HSE10,116.5676393698 chr9,136738864,136739065,HSE828,21.7259174217 chr9,136826692,136826892,HSE823,8.8396928196 chr9,138884709,138884909,HSE749,21.506795832 chr9,140320110,140320310,HSE558,17.6159627193 chrX,191631,191831,HSE375,22.299626784 chrX,197891,198101,HSE318,51.9353250981 chrX,199983,200184,HSE711,18.1602756116 chrX,9247138,9247338,HSE915,8.9889411466 chrX,9294553,9294753,HSE636,15.8583274764 chrX,13467472,13467672,HSE625,13.3123255447 chrX,22410208,22410409,HSE982,9.6853480348 chrX,29231637,29231837,HSE610,12.7761366615 chrX,30489623,30489824,HSE137,24.5548014378 chrX,31825117,31825317,HSE99,25.9398284883 chrX,36439734,36439934,HSE323,16.5354248431 chrX,36467884,36468084,HSE402,16.6156581564 chrX,40432956,40433156,HSE933,11.0828028555 chrX,40810144,40810344,HSE106,48.7539914758 chrX,49953599,49953799,HSE295,25.7542688302 chrX,57163623,57163823,HSE503,22.7827962324 chrX,114733851,114734051,HSE986,7.9927176453 chrX,124903682,124903882,HSE436,12.6648421132 chrX,128211486,128211686,HSE815,14.3611432709 chrX,128270781,128270981,HSE952,10.1358588237 chrX,130964620,130964822,HSE980,11.9103854587 ================================================ FILE: intro_awk_spring2021/data/example.fastq ================================================ @ERR117184_2.14044282 CTGGTGCTCAGTCATTTTGCTAGATTGTAGCTCACCATTGCCTCTCTGCCTGCATTGTGCACTCCAATCCCTCAGCAGCTACAAAAACACTTTGTCAACTCCAGATCGG + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117185_2.23793537 TTGTTCAATTTTTTTAGTAGTTTTAGAGTAATTAATGAGCTTTGAGGTCACTTAACAATAAAAGCATATTTTAAAGTAAAGGTTGCTGATCAACAGTCTAATTTTCATA + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117166_2.4649205 ACTTAACAATAAAAGCATATTTTAAAGTAAAGGTT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117185_2.949669 ATGCTTTTATTGTTAAGTGACCTCAAAGCTCATTAATTACTCTAAAACTACTAAAAAAATTGAACAAAAAAGTATTTCCAGTAATCAAGATTTTCTTCATGCCCTAGAT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117163_2.2292219 AAATCGTAAGTGAAGCAACAGAATTCAGAACTAAA + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117165_2.36526615 AACGCTAAGGTTATATTAAAATGTTTCTTATGTTG + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117174_2.23007391 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117175_2.11345437 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117176_2.30285510 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII @ERR117177_2.33680511 GCAAGTGGTTGGACTCAAGGAAGTTATAAATACCT + IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII ================================================ FILE: intro_awk_spring2021/data/hg38.genome ================================================ chrom size 1 248956422 2 242193529 3 198295559 4 190214555 5 181538259 6 170805979 7 159345973 X 156040895 8 145138636 9 138394717 11 135086622 10 133797422 12 133275309 13 114364328 14 107043718 15 101991189 16 90338345 17 83257441 18 80373285 20 64444167 19 58617616 Y 57227415 22 50818468 21 46709983 15_KI270905v1_alt 5161414 6_GL000256v2_alt 4929269 6_GL000254v2_alt 4827813 6_GL000251v2_alt 4795265 6_GL000253v2_alt 4677643 6_GL000250v2_alt 4672374 6_GL000255v2_alt 4606388 6_GL000252v2_alt 4604811 17_KI270857v1_alt 2877074 16_KI270853v1_alt 2659700 16_KI270728v1_random 1872759 17_GL000258v2_alt 1821992 5_GL339449v2_alt 1612928 14_KI270847v1_alt 1511111 17_KI270908v1_alt 1423190 14_KI270846v1_alt 1351393 5_KI270897v1_alt 1144418 7_KI270803v1_alt 1111570 19_GL949749v2_alt 1091841 19_KI270938v1_alt 1066800 19_GL949750v2_alt 1066390 19_GL949748v2_alt 1064304 19_GL949751v2_alt 1002683 19_GL949746v1_alt 987716 19_GL949752v1_alt 987100 8_KI270821v1_alt 985506 1_KI270763v1_alt 911658 6_KI270801v1_alt 870480 19_GL949753v2_alt 796479 19_GL949747v2_alt 729520 8_KI270822v1_alt 624492 4_GL000257v2_alt 586476 12_KI270904v1_alt 572349 4_KI270925v1_alt 555799 15_KI270852v1_alt 478999 15_KI270727v1_random 448248 9_KI270823v1_alt 439082 15_KI270850v1_alt 430880 1_KI270759v1_alt 425601 12_GL877876v1_alt 408271 Un_KI270442v1 392061 17_KI270862v1_alt 391357 15_GL383555v2_alt 388773 19_GL383573v1_alt 385657 4_KI270896v1_alt 378547 4_GL383528v1_alt 376187 17_GL383563v3_alt 375691 8_KI270810v1_alt 374415 1_GL383520v2_alt 366580 1_KI270762v1_alt 354444 15_KI270848v1_alt 327382 17_KI270909v1_alt 325800 14_KI270844v1_alt 322166 8_KI270900v1_alt 318687 10_GL383546v1_alt 309802 13_KI270838v1_alt 306913 8_KI270816v1_alt 305841 22_KI270879v1_alt 304135 8_KI270813v1_alt 300230 11_KI270831v1_alt 296895 15_GL383554v1_alt 296527 8_KI270811v1_alt 292436 18_GL383567v1_alt 289831 X_KI270880v1_alt 284869 8_KI270812v1_alt 282736 19_KI270921v1_alt 282224 17_KI270729v1_random 280839 17_JH159146v1_alt 278131 X_KI270913v1_alt 274009 6_KI270798v1_alt 271782 7_KI270808v1_alt 271455 22_KI270876v1_alt 263666 15_KI270851v1_alt 263054 22_KI270875v1_alt 259914 1_KI270766v1_alt 256271 19_KI270882v1_alt 248807 3_KI270778v1_alt 248252 15_KI270849v1_alt 244917 4_KI270786v1_alt 244096 12_KI270835v1_alt 238139 17_KI270858v1_alt 235827 19_KI270867v1_alt 233762 16_KI270855v1_alt 232857 8_KI270926v1_alt 229282 5_GL949742v1_alt 226852 3_KI270780v1_alt 224108 17_GL383565v1_alt 223995 2_KI270774v1_alt 223625 4_KI270790v1_alt 220246 11_KI270927v1_alt 218612 19_KI270932v1_alt 215732 11_KI270903v1_alt 214625 2_KI270894v1_alt 214158 14_GL000225v1_random 211173 Un_KI270743v1 210658 11_KI270832v1_alt 210133 7_KI270805v1_alt 209988 4_GL000008v2_random 209709 7_KI270809v1_alt 209586 19_KI270887v1_alt 209512 4_KI270789v1_alt 205944 3_KI270779v1_alt 205312 19_KI270914v1_alt 205194 19_KI270886v1_alt 204239 11_KI270829v1_alt 204059 14_GL000009v2_random 201709 21_GL383579v2_alt 201197 11_JH159136v1_alt 200998 19_KI270930v1_alt 200773 Un_KI270747v1 198735 18_GL383571v1_alt 198278 19_KI270920v1_alt 198005 6_KI270797v1_alt 197536 3_KI270935v1_alt 197351 17_KI270861v1_alt 196688 15_KI270906v1_alt 196384 5_KI270791v1_alt 195710 14_KI270722v1_random 194050 16_GL383556v1_alt 192462 13_KI270840v1_alt 191684 14_GL000194v1_random 191469 11_JH159137v1_alt 191409 19_KI270917v1_alt 190932 7_KI270899v1_alt 190869 19_KI270923v1_alt 189352 10_KI270825v1_alt 188315 19_GL383576v1_alt 188024 19_KI270922v1_alt 187935 Un_KI270742v1 186739 22_KI270878v1_alt 186262 19_KI270929v1_alt 186203 11_KI270826v1_alt 186169 6_KB021644v2_alt 185823 17_GL000205v2_random 185591 1_KI270765v1_alt 185285 19_KI270916v1_alt 184516 19_KI270890v1_alt 184499 3_KI270784v1_alt 184404 12_GL383551v1_alt 184319 20_KI270870v1_alt 183433 Un_GL000195v1 182896 1_GL383518v1_alt 182439 22_KI270736v1_random 181920 10_KI270824v1_alt 181496 14_KI270845v1_alt 180703 3_GL383526v1_alt 180671 13_KI270839v1_alt 180306 22_KI270733v1_random 179772 Un_GL000224v1 179693 10_GL383545v1_alt 179254 Un_GL000219v1 179198 5_KI270792v1_alt 179043 17_KI270860v1_alt 178921 19_GL000209v2_alt 177381 11_KI270830v1_alt 177092 9_KI270719v1_random 176845 Un_GL000216v2 176608 22_KI270928v1_alt 176103 1_KI270712v1_random 176043 6_KI270800v1_alt 175808 1_KI270706v1_random 175055 2_KI270776v1_alt 174166 18_KI270912v1_alt 174061 3_KI270777v1_alt 173649 5_GL383531v1_alt 173459 3_JH636055v2_alt 173151 14_KI270725v1_random 172810 5_KI270796v1_alt 172708 9_GL383541v1_alt 171286 19_KI270885v1_alt 171027 19_KI270919v1_alt 170701 19_KI270889v1_alt 170698 19_KI270891v1_alt 170680 19_KI270915v1_alt 170665 19_KI270933v1_alt 170537 19_KI270883v1_alt 170399 19_GL383575v2_alt 170222 19_KI270931v1_alt 170148 12_GL383550v2_alt 169178 13_KI270841v1_alt 169134 Un_KI270744v1 168472 18_KI270863v1_alt 167999 18_GL383569v1_alt 167950 12_GL877875v1_alt 167313 21_KI270874v1_alt 166743 3_KI270924v1_alt 166540 1_KI270761v1_alt 165834 3_KI270937v1_alt 165607 22_KI270734v1_random 165050 18_GL383570v1_alt 164789 5_KI270794v1_alt 164558 4_GL383527v1_alt 164536 Un_GL000213v1 164239 3_KI270936v1_alt 164170 3_KI270934v1_alt 163458 9_GL383539v1_alt 162988 3_KI270895v1_alt 162896 22_GL383582v2_alt 162811 3_KI270782v1_alt 162429 1_KI270892v1_alt 162212 Un_GL000220v1 161802 2_KI270767v1_alt 161578 2_KI270715v1_random 161471 2_KI270893v1_alt 161218 Un_GL000218v1 161147 18_GL383572v1_alt 159547 8_KI270817v1_alt 158983 4_KI270788v1_alt 158965 Un_KI270749v1 158759 7_KI270806v1_alt 158166 7_KI270804v1_alt 157952 18_KI270911v1_alt 157710 Un_KI270741v1 157432 17_KI270910v1_alt 157099 19_KI270884v1_alt 157053 19_GL383574v1_alt 155864 19_KI270888v1_alt 155532 3_GL000221v1_random 155397 11_GL383547v1_alt 154407 2_KI270716v1_random 153799 12_GL383553v2_alt 152874 6_KI270799v1_alt 152148 22_KI270731v1_random 150754 Un_KI270751v1 150742 Un_KI270750v1 148850 8_KI270818v1_alt 145606 X_KI270881v1_alt 144206 21_KI270873v1_alt 143900 2_GL383521v1_alt 143390 8_KI270814v1_alt 141812 12_GL383552v1_alt 138655 Un_KI270519v1 138126 2_KI270775v1_alt 138019 17_KI270907v1_alt 137721 Un_GL000214v1 137718 8_KI270901v1_alt 136959 2_KI270770v1_alt 136240 16_KI270854v1_alt 134193 8_KI270819v1_alt 133535 17_GL383564v2_alt 133151 2_KI270772v1_alt 133041 8_KI270815v1_alt 132244 5_KI270795v1_alt 131892 5_KI270898v1_alt 130957 20_GL383577v2_alt 128386 1_KI270708v1_random 127682 7_KI270807v1_alt 126434 5_KI270793v1_alt 126136 6_GL383533v1_alt 124736 2_GL383522v1_alt 123821 19_KI270918v1_alt 123111 12_GL383549v1_alt 120804 2_KI270769v1_alt 120616 4_KI270785v1_alt 119912 12_KI270834v1_alt 119498 7_GL383534v2_alt 119183 20_KI270869v1_alt 118774 21_GL383581v2_alt 116689 3_KI270781v1_alt 113034 17_KI270730v1_random 112551 Un_KI270438v1 112505 4_KI270787v1_alt 111943 18_KI270864v1_alt 111737 2_KI270771v1_alt 110395 1_GL383519v1_alt 110268 2_KI270768v1_alt 110099 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12_KI270836v1_alt 56134 19_KI270865v1_alt 52969 1_KI270764v1_alt 50258 Un_KI270589v1 44474 14_KI270726v1_random 43739 19_KI270866v1_alt 43156 22_KI270735v1_random 42811 1_KI270711v1_random 42210 Un_KI270745v1 41891 1_KI270714v1_random 41717 22_KI270732v1_random 41543 1_KI270713v1_random 40745 Un_KI270754v1 40191 1_KI270710v1_random 40176 12_KI270837v1_alt 40090 9_KI270717v1_random 40062 14_KI270724v1_random 39555 9_KI270720v1_random 39050 14_KI270723v1_random 38115 9_KI270718v1_random 38054 Un_KI270317v1 37690 13_KI270842v1_alt 37287 Y_KI270740v1_random 37240 Un_KI270755v1 36723 8_KI270820v1_alt 36640 1_KI270707v1_random 32032 Un_KI270579v1 31033 Un_KI270752v1 27745 Un_KI270512v1 22689 Un_KI270322v1 21476 M 16569 Un_GL000226v1 15008 Un_KI270311v1 12399 Un_KI270366v1 8320 Un_KI270511v1 8127 Un_KI270448v1 7992 Un_KI270521v1 7642 Un_KI270581v1 7046 Un_KI270582v1 6504 Un_KI270515v1 6361 Un_KI270588v1 6158 Un_KI270591v1 5796 Un_KI270522v1 5674 Un_KI270507v1 5353 Un_KI270590v1 4685 Un_KI270584v1 4513 Un_KI270320v1 4416 Un_KI270382v1 4215 Un_KI270468v1 4055 Un_KI270467v1 3920 Un_KI270362v1 3530 Un_KI270517v1 3253 Un_KI270593v1 3041 Un_KI270528v1 2983 Un_KI270587v1 2969 Un_KI270364v1 2855 Un_KI270371v1 2805 Un_KI270333v1 2699 Un_KI270374v1 2656 Un_KI270411v1 2646 Un_KI270414v1 2489 Un_KI270510v1 2415 Un_KI270390v1 2387 Un_KI270375v1 2378 Un_KI270420v1 2321 Un_KI270509v1 2318 Un_KI270315v1 2276 Un_KI270302v1 2274 Un_KI270518v1 2186 Un_KI270530v1 2168 Un_KI270304v1 2165 Un_KI270418v1 2145 Un_KI270424v1 2140 Un_KI270417v1 2043 Un_KI270508v1 1951 Un_KI270303v1 1942 Un_KI270381v1 1930 Un_KI270529v1 1899 Un_KI270425v1 1884 Un_KI270396v1 1880 Un_KI270363v1 1803 Un_KI270386v1 1788 Un_KI270465v1 1774 Un_KI270383v1 1750 Un_KI270384v1 1658 Un_KI270330v1 1652 Un_KI270372v1 1650 Un_KI270548v1 1599 Un_KI270580v1 1553 Un_KI270387v1 1537 Un_KI270391v1 1484 Un_KI270305v1 1472 Un_KI270373v1 1451 Un_KI270422v1 1445 Un_KI270316v1 1444 Un_KI270338v1 1428 Un_KI270340v1 1428 Un_KI270583v1 1400 Un_KI270334v1 1368 Un_KI270429v1 1361 Un_KI270393v1 1308 Un_KI270516v1 1300 Un_KI270389v1 1298 Un_KI270466v1 1233 Un_KI270388v1 1216 Un_KI270544v1 1202 Un_KI270310v1 1201 Un_KI270412v1 1179 Un_KI270395v1 1143 Un_KI270376v1 1136 Un_KI270337v1 1121 Un_KI270335v1 1048 Un_KI270378v1 1048 Un_KI270379v1 1045 Un_KI270329v1 1040 Un_KI270419v1 1029 Un_KI270336v1 1026 Un_KI270312v1 998 Un_KI270539v1 993 Un_KI270385v1 990 Un_KI270423v1 981 Un_KI270392v1 971 Un_KI270394v1 970 ================================================ FILE: intro_awk_spring2021/data/sample.txt ================================================ my dog is brown ================================================ FILE: intro_awk_spring2021/index.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "## Manipulating files with AWK and grep\n", "### Bioinformatics Coffee Hour - April 20, 2021\n", "### Author: Danielle Khost\n" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "\n", "## Manipulating files with grep\n", "--------\n", "In this lesson, we will explore how we can use basic command line tools to parse, subset, and rearrange common file types you will come across in bioinformatics.\n", "\n", "**grep** is a powerful command-line search tools that is included as part of most Unix-like systems. It is one of the most useful tools in bioinformatics! At the most basic level, grep searches for a string of characters that match a pattern and will print lines containing a match. Basic syntax is: \n", "\n", "`grep 'pattern' file_to_search` \n", "\n", "By default, grep will match any part of the string, so for example:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep 'dog' data/sample.txt" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This will match the line, as will:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep 'do' data/sample.txt" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "It is important to be mindful of partial matching, as you can end up selecting lines that you do not intend! This is just the most basic use of grep however, and there are a huge number of ways to modify its behavior. Here are just a few useful examples:\n", "\n", "`grep -w` matches *entire words*.\n", "- So in the above example: \n", "`grep -w 'dog' data/sample.txt` would match the string, but `grep -w 'do' data/sample.txt` would not.\n", "\n", "`grep -i` allows case-insensitive matches\n", "- In the above example, `grep -i 'DOG'` would still match the line\n", "\n", "`grep -v` *inverts*, returning lines that *do not* match the pattern.\n", "\n", "`grep -o` returns only the matching words, not the entire line.\n", "\n", "`grep -c` counts the number of lines that match the pattern.\n", "- Equivalent to `grep 'pattern' file | wc -l`\n", "\n", "Print lines before/after a match:\n", "- `grep -A [n]` returns matching line and *n* lines after match\n", "- `grep -B [n]` returns matching line and *n* lines before match\n", "- `grep -C [n]` returns matching line and *n* lines before *and* after match\n", "\n", "---\n", "\n", "The real strength of grep is that we can combine these different arguments together. For instance, let's say we have a single-line fasta file and we want to quickly pull out the X chromosome:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep -w -A 1 '>X' data/dmel-subset-chromosomes.fasta" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This code will match line that start with \">X\" (using the -w flag to match whole word only) and will also print the line *after* the match as well using the `-A 1` flag (i.e. the sequence line of our fasta file; note this will not work if fasta file is multi-line!).\n", "\n", "There are many other functions of grep! When in doubt, remember you can check the help page using `man grep`...or just by using google :)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Pattern matching with regular expressions\n", "Regular expressions, aka \"regex\", are patterns that describe sets of strings. In other words, they allow you to match complex patterns with grep, not just exact matches. Regex is extremely powerful, but can also get (very) complicated, so we'll just stick to a few basic uses.\n", "\n", "Regex has certain meta-characters that are reserved for special uses:\n", "\n", "```\n", "^: matches pattern at start of string\n", "$: matches pattern at end of string\n", ".: matches any character except new lines\n", "[]: matches any of enclose characters\n", "[^]: matches any characters *except* ones enclosed (note: is different from ^)\n", "\\: \"escapes\" meta-characters, allows literal matching\n", "```\n", "\n", "Note that if we want to match any of these special characters literally, we would need to use a \"\\\\\" to escape it first. So if we wanted to literally match a period (\".\") character we would run:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep '\\.' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "One example of how regex can come in handy is using the ^ special character to quickly count how many sequences are in a FASTA file, which we would do as follows:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep -c '^>' data/dmel-subset-chromosomes.fasta" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This command matches lines in the FASTA file that start with a \">\" character, i.e. the header lines, and uses the -c argument to count how many matches!\n", "\n", "---" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## What is awk?\n", "Invented in the 1970's, [awk](https://en.wikipedia.org/wiki/AWK) is a scripting language included in most Unix-like operating systems. It specializes in one-liner programs and manipulating text files.\n", "\n", "In many cases, if you're parsing information from a text file (such as a [BED](https://en.wikipedia.org/wiki/BED_(file_format)) file, [FASTA](https://en.wikipedia.org/wiki/FASTA) file, etc.), you could write a Python script...or you could do it with awk in a single line!\n", "\n", "### Syntax\n", "awk scripts are organized as:\n", "\n", "`awk 'pattern { action; other action }' file`\n", "\n", "Meaning that every time that the pattern is true, awk will execute the action in the brackets.\n", "If no pattern is specified, the action will be taken for every line in the input file, e.g. the following command prints every line:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "The two most important patterns are `BEGIN` and `END`, which tell the action to take place before any lines are read and after the last line." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{sum=0} {sum+=1} END {print sum}' data/hg38.genome" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ " The above line sets a variable at the start of the script, adds 1 to it every line, then prints its value at the end." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "If a variable hasn't been initialized, it is treated as 0 in numeric expressions, and an empty string in string expressions—awk will not print an error!\n", "So the following awk script also prints the number of lines in the file data/hg38.genome:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{sum+=1} END {print sum}' data/hg38.genome" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Input and output\n", "Input to awk is split into **records** and **fields**.\n", "- By default, **records** are separated by newline character, i.e # of records = # of lines in input file\n", "- Each record is subdivided into **fields**, i.e. columns, as determined by the field separator (see below)\n", "\n", "There are several important built-in variable in awk.\n", "The fields (columns) of each record are referred to by `$number`, so the first column would be `$1`, second would be `$2`, etc. `$0` refers to the entire record.\n", "\n", "So to print the second column of each line in the file, we'd use:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print $2}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "And if we wanted to print the second then the first:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print $2,$1}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note that when the different fields are separated with commas in the `print` statement, they are joined by the output field separator (the **OFS** variable, described below), which is by default a space.\n", "If the comma is omitted between fields (e.g., `awk '{print $2 $1}'`, they are concatenated without a separator.\n", "\n", "We can also print strings using using quotation marks:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '{print \"First column:\" $1}' data/hg38.genome | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Which for every line of the file will print the text \"First column:\" followed by the value in the first field." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "---\n", "awk has several other built-in variables that are very useful for parsing text, including:\n", "\n", "| | |\n", "---|---|\n", "| **FS** | field separator (default: white space) |\n", "| **OFS** | output field separator, i.e. what character separates fields when printing|\n", "| **RS** | record separator, i.e. what character records are split on (default: new line) |\n", "| **ORS** | output record separator |\n", "| **NR** | number of records in input (# lines by default) |\n", "\n", "Assigning to a field causes the entire record ($0) to be recomputed using **OFS**. We can use this to convert between file formats, e.g. make a comma-separated text file into a tab-separated file. First, let's look at the first few lines on our comma-separated file using `head`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/enhancers.csv" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now let's convert the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\",\" ; OFS=\"\\t\"} {$1 = $1; print $0}' data/enhancers.csv | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Conditionals and pattern matching\n", "Like other programming languages, awk allows conditional matching with if/else statements.\n", "\n", "`awk '{if(condition) action; else other_action}'`\n", "\n", "awk uses the following conditional operators:\n", "\n", "| | |\n", "|-|-|\n", "|==|equal to|\n", "|!=|not equal to|\n", "|>|greater than|\n", "|>=|greater than or equal to|\n", "|<|less than|\n", "|<=|less than or equal to|\n", "|&&|AND|\n", "| \\|\\| |OR|\n", "| ! | NOT |\n", "\n", "In addition, awk also supports string matching using regular expressions, using the following expressions:\n", "\n", "| | |\n", "|-|-|\n", "|\\~|matches|\n", "|!~|does not match|\n", "\n", "For string matching, the pattern being matched must be enclosed by slashes, like so:\n", "\n", "`awk '{if($1 ~ /pattern/) print}'`\n", "\n", "Note that if an action isn't specified, the default action is `{print}`, so the previous awk command is equivalent to the following, which specifies only a pattern expression:\n", "\n", "`awk '$1 ~ /pattern/'`\n", "\n", "---\n", "### Example uses:\n", "\n", "- Count number of sequences in a FASTQ file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'END{print NR/4}' data/example.fastq" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: this is technically safer than using grep, as you don't have to worry about accidentally counting the quality line.\n", "\n", "- Only print annotations on a specific scaffold (chr1) that fall between 1Mb and 2Mb from a BED annotation file (a common file type that lists genomic coordinates of certain features). First let's look at the first few lines of the file using `head`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now subset the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\";OFS=\"\\t\"} {if($1 == \"chr1\" && $2 >=1000000 && $2 <= 2000000) print}' data/Homo_sapiens_ucscGenes.subset.bed | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: when we specify that we only want annotations from chr1, we're using exact match (`== \"chr1\"`) and not pattern match (`~ /chr1/`)...why is this??" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Another common type of annotation file is a Genome Feature File (GFF), which also lists coordinates. Let's look at the first few lines of the file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "head data/Homo_sapiens.GRCh38.subset.gff3" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now let's subset the file and only print lines that match the string \"exon\" in their third column:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"} {if($3 ~ /exon/) print $0}' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Convert from GFF (genome feature file) to BED file " ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "grep -v '^#' data/Homo_sapiens.GRCh38.subset.gff3 | awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} {print $1,$4-1,$5}' | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "**Note**: Annoyingly, BED and GFF files have different coordinate systems, i.e. BED start coordinate is 0 based, half-open, GFF is 1-based inclusive! Also, we are first using grep to skip the header lines in the GFF file.\n", "\n", "Alternatively, you could do the whole thing with only awk, no grep required:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} !/^#/ {print $1,$4-1,$5}' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "With this command, `!/^#/` is a pattern (like `BEGIN` or `END`) that tell awk to execute the print statement when the start of the line does not match a `#`. Use whichever makes the most sense to you!" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Practice\n", "Using awk:\n", "\n", "* Pull out only the CDS annotations (i.e. has \"CDS\" in the 3rd column) from the GFF file data/Homo_sapiens.GRCh38.subset.gff3 and output them in BED format" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Try it*" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '...' data/Homo_sapiens.GRCh38.subset.gff3 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Solution*" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "tags": [] }, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; OFS=\"\\t\"} {if($3 ~ /CDS/) print $1,$4-1,$5}' data/Homo_sapiens.GRCh38.subset.gff3" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "- Calculate the average length of gene annotations from the file data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Try it*" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "awk '...' data/Homo_sapiens_ucscGenes.subset.bed" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Solution*" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "tags": [] }, "outputs": [], "source": [ "awk 'BEGIN{FS=\"\\t\"; sum=0} {len=$3-$2; sum=sum+len} END{print sum/NR}' data/Homo_sapiens_ucscGenes.subset.bed" ] } ], "metadata": { "kernelspec": { "display_name": "Python 3", "language": "python", "name": "python3" }, "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.9.1" } }, "nbformat": 4, "nbformat_minor": 4 } ================================================ FILE: intro_data_science/part_1/binder/requirements.txt ================================================ pandas==1.2.0 matplotlib==3.3.3 numpy==1.19.5 ================================================ FILE: intro_data_science/part_1/ds_part_1.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "# A Brief Introduction to NumPy\n", "### \"...the fundamental package for scientific computing with Python.\" - numpy.org\n", "\n", "In this notebook, we will cover the basics of NumPy, a package that is the basis for many other libraries in the data science ecosystem. Let's get started." ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [], "source": [ "import numpy as np\n", "from IPython.display import Image\n", "import time\n", "from sys import getsizeof" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# 1. NumPy Arrays\n", "The array data structure is the backbone of the NumPy library. They can be single-dimensional (vectors), two-dimensional (matrices), or multi-dimensional for more complex tasks.\n", "\n", "In many ways, they are similar to Python lists." ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "a\n['b' 'c']\na\nb\nc\nd\ne\nf\n" ] } ], "source": [ "a = ['a', 'b', 'c', 'd', 'e', 'f']\n", "b = np.array(['a', 'b', 'c', 'd', 'e', 'f'])\n", "\n", "# Accessible by index\n", "print(b[0])\n", "\n", "# Sliceable\n", "print(b[1:3])\n", "\n", "# Iterable\n", "for letter in b:\n", " print(letter)" ] }, { "source": [ "So why use NumPy arrays at all? One word: performance! Generally speaking, Python lists take up more space and require more computation than NumPy arrays. Let's take a look at the size differences." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "8697456\n8000096\n" ] } ], "source": [ "n_elements = 1_000_000\n", "# Create using list comprehension\n", "python_list = [x for x in range(n_elements)]\n", "print(getsizeof(python_list))\n", "\n", "# Create with existing python list\n", "np_arr = np.array(python_list)\n", "print(getsizeof(np_arr))" ] }, { "source": [ "Now let's look at the speed differences." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 6, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "0.07268199999999991\n0.004588999999999954\n" ] } ], "source": [ "start = time.process_time()\n", "# Add 100 to every element in the Python list\n", "python_list_mod = [x + 100 for x in python_list]\n", "python_time = time.process_time() - start\n", "print(python_time)\n", "\n", "# Add 100 to every element in the Numpy array\n", "start = time.process_time()\n", "np_arr_mod = np_arr + 100\n", "np_time = time.process_time() - start\n", "print(np_time)" ] }, { "cell_type": "code", "execution_count": 7, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "image/png": 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"text/plain": [ "" ] }, "metadata": {}, "execution_count": 7 } ], "source": [ "Image('python_memory1.png')" ] }, { "source": [ "If NumPy arrays are more efficient computationally and in regards to space, why not use them all the time? There are some constraints, most notably, all of their items must be of the same type. [NumPy Array](https://numpy.org/doc/stable/reference/generated/numpy.array.html#numpy.array)" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 8, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "[1, 'a', 0.222, 'hello from inside the list!']\n['1' 'a' '0.222' 'hello from inside the list!']\n" ] } ], "source": [ "python_list = [1, 'a', 0.222, 'hello from inside the list!']\n", "np_arr = np.array(python_list)\n", "print(python_list)\n", "print(np_arr)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# 1.1 Creating\n", "NumPy arrays are created with existing data (standard python lists or lists of lists) or by using a collection of built-in methods." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.1 Existing Data" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .array()\n", "Use python lists (or lists of lists) as input." ] }, { "cell_type": "code", "execution_count": 2, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([1, 2, 3, 4, 5])" ] }, "metadata": {}, "execution_count": 2 } ], "source": [ "std_list = [1, 2, 3, 4, 5]" ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([[1, 2, 3],\n", " [4, 5, 6],\n", " [7, 8, 9]])" ] }, "metadata": {}, "execution_count": 3 } ], "source": [ "std_matrix = [[1, 2, 3], [4, 5, 6], [7, 8, 9]]" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: the .array() method is a convenience function for constructing objects of the class ndarray. While it is possible to call .ndarray() directly, it is specifically regarded as an anti-pattern by the NumPy documentation." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.2 Fixed Values" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .zeros(), .ones()\n", "Return a new array of given shape and type, filled with zeros or ones." ] }, { "cell_type": "code", "execution_count": 4, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([0., 0., 0.])" ] }, "metadata": {}, "execution_count": 4 } ], "source": [] }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1.])" ] }, "metadata": {}, "execution_count": 5 } ], "source": [] }, { "cell_type": "code", "execution_count": 8, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([[0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.]])" ] }, "metadata": {}, "execution_count": 8 } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: the numbers have periods after them to indicate that these are floating point numbers." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .full()\n", "Return a new array of given shape and type, filled with fill_value." ] }, { "cell_type": "code", "execution_count": 10, "metadata": { "scrolled": true }, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([[72, 72, 72, 72],\n", " [72, 72, 72, 72],\n", " [72, 72, 72, 72],\n", " [72, 72, 72, 72]])" ] }, "metadata": {}, "execution_count": 10 } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.3 Range" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .arange()\n", "Return evenly spaced values within a given interval. Notice that the output is inclusive of the first number parameter and exclusive of the second." ] }, { "cell_type": "code", "execution_count": 10, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([0, 1, 2, 3, 4, 5, 6, 7, 8, 9])" ] }, "execution_count": 10, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 11, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 2, 4, 6, 8])" ] }, "execution_count": 11, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 12, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 5, 10, 15])" ] }, "execution_count": 12, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .linspace()\n", "Return evenly spaced numbers over a specified interval. Notice that the output is inclusive of both the first and second number parameters." ] }, { "cell_type": "code", "execution_count": 13, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0., 5., 10., 15., 20.])" ] }, "execution_count": 13, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 14, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([0. , 0.05263158, 0.10526316, 0.15789474, 0.21052632,\n", " 0.26315789, 0.31578947, 0.36842105, 0.42105263, 0.47368421,\n", " 0.52631579, 0.57894737, 0.63157895, 0.68421053, 0.73684211,\n", " 0.78947368, 0.84210526, 0.89473684, 0.94736842, 1. ])" ] }, "execution_count": 14, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: the main difference between .linspace() and .arange() is that with .linspace() you have precise control over the end value, whereas with .arange() you can specify the increments explicitly." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .logspace()\n", "Return numbers spaced evenly on a log scale." ] }, { "cell_type": "code", "execution_count": 15, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 100. , 215.443469 , 464.15888336, 1000. ])" ] }, "execution_count": 15, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 25, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([4. , 5.0396842 , 6.34960421, 8. ])" ] }, "execution_count": 25, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.4 Random" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .rand()\n", "Create an array of the given shape and populate it with random samples from a uniform distribution over 0,1." ] }, { "cell_type": "code", "execution_count": 27, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0.7125801811390514" ] }, "execution_count": 27, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 18, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0.91155984, 0.98323076, 0.60797164, 0.48538736])" ] }, "execution_count": 18, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 19, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[0.56443505, 0.13692564, 0.4367128 ],\n", " [0.66786468, 0.86782966, 0.88696338],\n", " [0.05537221, 0.54016645, 0.9654299 ],\n", " [0.19105141, 0.63037385, 0.51600478],\n", " [0.53737417, 0.76494641, 0.61205375],\n", " [0.09621612, 0.37848038, 0.60698676],\n", " [0.47772402, 0.61733058, 0.75352393]])" ] }, "execution_count": 19, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .randn()\n", "Return a sample (or samples) from the “standard normal” distribution." ] }, { "cell_type": "code", "execution_count": 39, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0.36910179629879897" ] }, "execution_count": 39, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 46, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([-0.08320502, -0.69825957, 0.80139519, 0.53540454, 0.78953503])" ] }, "execution_count": 46, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: .rand() is from a uniform distribution, whereas .randn() is from the standard **normal** distribution." ] }, { "cell_type": "code", "execution_count": 55, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 1.33667905, 0.46529544, 1.51069451, 0.69167808, 1.60270943],\n", " [ 1.62614154, -0.10463841, -0.999014 , -0.70942201, -0.15136812],\n", " [-0.70328834, -0.48982338, 2.03118893, 2.32867839, 2.08885297]])" ] }, "execution_count": 55, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .randint()\n", "Return random integers from low (inclusive) to high (exclusive)." ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "5" ] }, "execution_count": 3, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 66, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([4, 5, 5, 6, 1, 2, 1])" ] }, "execution_count": 66, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 67, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[9, 3],\n", " [6, 8],\n", " [7, 6]])" ] }, "execution_count": 67, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.2 Attributes and Methods" ] }, { "cell_type": "code", "execution_count": 82, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[2, 0, 8, 7, 8, 4, 6],\n", " [3, 5, 6, 6, 7, 6, 2]])" ] }, "execution_count": 82, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .shape\n", "Tuple of array dimensions. Note: this is an attribute NOT a method." ] }, { "cell_type": "code", "execution_count": 83, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "(2, 7)" ] }, "execution_count": 83, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .reshape()\n", "Gives a new shape to an array without changing its data. Note: this happens 'in place' (does not return new values)." ] }, { "cell_type": "code", "execution_count": 88, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[2, 0],\n", " [8, 7],\n", " [8, 4],\n", " [6, 3],\n", " [5, 6],\n", " [6, 7],\n", " [6, 2]])" ] }, "execution_count": 88, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 85, "metadata": {}, "outputs": [ { "ename": "ValueError", "evalue": "cannot reshape array of size 14 into shape (12,4)", "output_type": "error", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mValueError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[0;32m----> 1\u001b[0;31m \u001b[0marr\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mreshape\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;36m12\u001b[0m\u001b[0;34m,\u001b[0m\u001b[0;36m4\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;31mValueError\u001b[0m: cannot reshape array of size 14 into shape (12,4)" ] } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .newaxis\n", "Alternate syntax." ] }, { "cell_type": "code", "execution_count": 92, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[[2, 0, 8, 7, 8, 4, 6],\n", " [3, 5, 6, 6, 7, 6, 2]]])" ] }, "execution_count": 92, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .dtype\n", "The type of data in the array." ] }, { "cell_type": "code", "execution_count": 80, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "dtype('int64')" ] }, "execution_count": 80, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .astype()\n", "Casts values to a specified type." ] }, { "cell_type": "code", "execution_count": 83, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[9, 5, 7, 3, 6, 8, 9],\n", " [9, 6, 1, 1, 4, 2, 3]], dtype=int8)" ] }, "execution_count": 83, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 81, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[9.+0.j, 5.+0.j, 7.+0.j, 3.+0.j, 6.+0.j, 8.+0.j, 9.+0.j],\n", " [9.+0.j, 6.+0.j, 1.+0.j, 1.+0.j, 4.+0.j, 2.+0.j, 3.+0.j]])" ] }, "execution_count": 81, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: in mathematics i is used to denote imaginary numbers, but in Python (and many other languages) j is used because i tends to indicate the current value in a system. " ] }, { "cell_type": "code", "execution_count": 114, "metadata": {}, "outputs": [], "source": [] }, { "cell_type": "code", "execution_count": 115, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ True, True, True, True],\n", " [ True, True, True, True],\n", " [ True, True, True, True],\n", " [ True, True, True, True]])" ] }, "execution_count": 115, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.3 Indexing" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 1.3.1 One-dimensional" ] }, { "cell_type": "code", "execution_count": 34, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20])" ] }, "execution_count": 34, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 35, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "10" ] }, "execution_count": 35, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the value at index 5 (the sixth element)" ] }, { "cell_type": "code", "execution_count": 36, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([2, 4, 6, 8])" ] }, "execution_count": 36, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get a slice of the array from index 1 (inclusive) to index 5 (exclusive)" ] }, { "cell_type": "code", "execution_count": 37, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 8, 10, 12, 14, 16, 18, 20])" ] }, "execution_count": 37, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get a slice of the array from index 4 to the end" ] }, { "cell_type": "code", "execution_count": 38, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "20" ] }, "execution_count": 38, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the last element in the array" ] }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [], "source": [ "# Reverse the array" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 1.3.2 Two-dimensional" ] }, { "cell_type": "code", "execution_count": 66, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 2, 3],\n", " [ 4, 5, 6, 7],\n", " [ 8, 9, 10, 11]])" ] }, "execution_count": 66, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 67, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 1, 2, 3])" ] }, "execution_count": 67, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the first row" ] }, { "cell_type": "code", "execution_count": 69, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "5" ] }, "execution_count": 69, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the second element of the second row" ] }, { "cell_type": "code", "execution_count": 70, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "5" ] }, "execution_count": 70, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Alternative syntax" ] }, { "cell_type": "code", "execution_count": 71, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[0, 1, 2, 3],\n", " [4, 5, 6, 7]])" ] }, "execution_count": 71, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get first and second rows" ] }, { "cell_type": "code", "execution_count": 72, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 4])" ] }, "execution_count": 72, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get first element of both first and second rows" ] }, { "cell_type": "code", "execution_count": 73, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[0],\n", " [4]])" ] }, "execution_count": 73, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Maintain shape" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 1.3.3 Fancy Indexing" ] }, { "cell_type": "code", "execution_count": 86, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "[4, 6, 14, 16]" ] }, "execution_count": 86, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 87, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 4, 6, 14, 16])" ] }, "execution_count": 87, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 88, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 4, 6],\n", " [14, 16]])" ] }, "execution_count": 88, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Output of fancy indexing" ] }, { "cell_type": "code", "execution_count": 94, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([2, 7])" ] }, "execution_count": 94, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 96, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([2, 3])" ] }, "execution_count": 96, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Notice if second row value is not provided, NumPy compensates" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.4 Selection\n", "We will see this syntax mirrored in the Pandas library." ] }, { "cell_type": "code", "execution_count": 20, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([1, 2, 3, 4])" ] }, "execution_count": 20, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 24, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([False, False, True, True])" ] }, "execution_count": 24, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 25, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([3, 4])" ] }, "execution_count": 25, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 32, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,\n", " 17, 18, 19])" ] }, "execution_count": 32, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 34, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([12, 13, 14, 15, 16, 17, 18, 19])" ] }, "execution_count": 34, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "code", "execution_count": 35, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 1, 2, 3, 4])" ] }, "execution_count": 35, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# 2. Operations\n", "One of the most powerful features of NumPy arrays is that operations are vectorized." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 2.1 Arithmetic\n", "Arithmetic operations work on NumPy arrays." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 2.1.1 One-dimensional" ] }, { "cell_type": "code", "execution_count": 4, "metadata": {}, "outputs": [], "source": [ "arr_1d = np.arange(0,11)" ] }, { "cell_type": "code", "execution_count": 63, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15])" ] }, "execution_count": 63, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Add five to each item" ] }, { "cell_type": "code", "execution_count": 6, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([-12, -11, -10, -9, -8, -7, -6, -5, -4, -3, -2])" ] }, "metadata": {}, "execution_count": 6 } ], "source": [ "# Subtract 12 from each item" ] }, { "cell_type": "code", "execution_count": 64, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20])" ] }, "execution_count": 64, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Multiply each item by 2" ] }, { "cell_type": "code", "execution_count": 101, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0. , 0.25, 0.5 , 0.75, 1. , 1.25, 1.5 , 1.75, 2. , 2.25, 2.5 ])" ] }, "execution_count": 101, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Divide each item by 4" ] }, { "cell_type": "code", "execution_count": 102, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 0, 0, 0, 1, 1, 1, 1, 2, 2, 2])" ] }, "execution_count": 102, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Floor division on each item" ] }, { "cell_type": "code", "execution_count": 103, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 1, 8, 27, 64, 125, 216, 343, 512, 729, 1000])" ] }, "execution_count": 103, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Raise each item to the third power" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 2.1.2 Two-dimensional" ] }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 2, 3, 4],\n", " [ 5, 6, 7, 8, 9],\n", " [10, 11, 12, 13, 14]])" ] }, "execution_count": 5, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_2d = np.arange(15).reshape((3,5))" ] }, { "cell_type": "code", "execution_count": 110, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 2, 4, 6, 8],\n", " [10, 12, 14, 16, 18],\n", " [20, 22, 24, 26, 28]])" ] }, "execution_count": 110, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Multiply each item by 2" ] }, { "cell_type": "code", "execution_count": 111, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 4, 9, 16],\n", " [ 25, 36, 49, 64, 81],\n", " [100, 121, 144, 169, 196]])" ] }, "execution_count": 111, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Raise each item to the power of 2" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 2.1.3 Multiple values\n", "These operations work with multiple values." ] }, { "cell_type": "code", "execution_count": 6, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([1, 2, 3, 4, 5])" ] }, "execution_count": 6, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_mult = np.array([1,2,3,4,5])" ] }, { "cell_type": "code", "execution_count": 7, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 2, 6, 12, 20],\n", " [ 5, 12, 21, 32, 45],\n", " [10, 22, 36, 52, 70]])" ] }, "execution_count": 7, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_2d * arr_mult" ] }, { "cell_type": "code", "execution_count": 47, "metadata": {}, "outputs": [], "source": [ "arr_mult_2 = np.array([1,2,3])" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 2.2 Ufuncs\n", "Universal functions. For more information, visit: https://docs.scipy.org/doc/numpy/reference/ufuncs.html" ] }, { "cell_type": "code", "execution_count": 75, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 1, 2, 3, 4, 5, 6, 7, 8, 9, 10])" ] }, "execution_count": 75, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr = np.arange(1,11)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .sum()\n", "Sum of array elements over a given axis." ] }, { "cell_type": "code", "execution_count": 105, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "70" ] }, "execution_count": 105, "metadata": {}, "output_type": "execute_result" } ], "source": [] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .sqrt()\n", "Return the non-negative square-root of an array, element-wise." ] }, { "cell_type": "code", "execution_count": 76, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "3.0" ] }, "execution_count": 76, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.sqrt(9)" ] }, { "cell_type": "code", "execution_count": 77, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([1. , 1.41421356, 1.73205081, 2. , 2.23606798,\n", " 2.44948974, 2.64575131, 2.82842712, 3. , 3.16227766])" ] }, "execution_count": 77, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.sqrt(arr)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .power()\n", "First array elements raised to powers from second array, element-wise." ] }, { "cell_type": "code", "execution_count": 79, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "9" ] }, "execution_count": 79, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.power(3,2)" ] }, { "cell_type": "code", "execution_count": 81, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 1, 4, 9, 16, 25, 36, 49, 64, 81, 100])" ] }, "execution_count": 81, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.power(arr, 2)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .min(), .max()" ] }, { "cell_type": "code", "execution_count": 65, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([3, 5, 4, 0, 3, 5, 5, 9, 7, 7])" ] }, "execution_count": 65, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr = np.random.randint(0,10, 10)" ] }, { "cell_type": "code", "execution_count": 67, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0" ] }, "execution_count": 67, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.min(arr)" ] }, { "cell_type": "code", "execution_count": 68, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0" ] }, "execution_count": 68, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.min()" ] }, { "cell_type": "code", "execution_count": 69, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "9" ] }, "execution_count": 69, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.max(arr)" ] }, { "cell_type": "code", "execution_count": 70, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "9" ] }, "execution_count": 70, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.max()" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 2.3 Broadcasting\n", "The term broadcasting describes how numpy treats arrays with different shapes during arithmetic operations. Subject to certain constraints, the smaller array is “broadcast” across the larger array so that they have compatible shapes. (NumPy documentation) " ] }, { "cell_type": "code", "execution_count": 93, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 2, 3, 4],\n", " [ 5, 6, 7, 8, 9],\n", " [10, 11, 12, 13, 14],\n", " [15, 16, 17, 18, 19]])" ] }, "execution_count": 93, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one = np.arange(0,20).reshape(4,5)" ] }, { "cell_type": "code", "execution_count": 94, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 11, 12, 13, 14],\n", " [15, 16, 17, 18, 19],\n", " [20, 21, 22, 23, 24],\n", " [25, 26, 27, 28, 29]])" ] }, "execution_count": 94, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + 10" ] }, { "cell_type": "code", "execution_count": 96, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 11, 12, 13, 14],\n", " [15, 16, 17, 18, 19],\n", " [20, 21, 22, 23, 24],\n", " [25, 26, 27, 28, 29]])" ] }, "execution_count": 96, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + np.array([10])" ] }, { "cell_type": "code", "execution_count": 97, "metadata": {}, "outputs": [ { "ename": "ValueError", "evalue": "operands could not be broadcast together with shapes (4,5) (2,) ", "output_type": "error", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mValueError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[0;32m----> 1\u001b[0;31m \u001b[0marr_one\u001b[0m \u001b[0;34m+\u001b[0m \u001b[0mnp\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0marray\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m[\u001b[0m\u001b[0;36m10\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0;36m20\u001b[0m\u001b[0;34m]\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;31mValueError\u001b[0m: operands could not be broadcast together with shapes (4,5) (2,) " ] } ], "source": [ "arr_one + np.array([10, 20])" ] }, { "cell_type": "code", "execution_count": 99, "metadata": {}, "outputs": [], "source": [ "arr_two = np.array([10,20,30,40,50])" ] }, { "cell_type": "code", "execution_count": 100, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 21, 32, 43, 54],\n", " [15, 26, 37, 48, 59],\n", " [20, 31, 42, 53, 64],\n", " [25, 36, 47, 58, 69]])" ] }, "execution_count": 100, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + arr_two" ] }, { "cell_type": "code", "execution_count": 101, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "(4, 5)" ] }, "execution_count": 101, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one.shape" ] }, { "cell_type": "code", "execution_count": 102, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "(5,)" ] }, "execution_count": 102, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_two.shape" ] }, { "cell_type": "code", "execution_count": 103, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10],\n", " [20],\n", " [30],\n", " [40],\n", " [50]])" ] }, "execution_count": 103, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_two.reshape(5,1)" ] }, { "cell_type": "code", "execution_count": 104, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 21, 32, 43, 54],\n", " [15, 26, 37, 48, 59],\n", " [20, 31, 42, 53, 64],\n", " [25, 36, 47, 58, 69]])" ] }, "execution_count": 104, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + arr_two" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Array comparison begins with the trailing dimensions and subsequently works its way foward. Two array dimensions are compatible when:\n", "- they are equal, or\n", "- one of them is 1\n", "(NumPy documentation)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "https://jakevdp.github.io/PythonDataScienceHandbook/02.05-computation-on-arrays-broadcasting.html" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Plot sin and cos on the same graph, using matplotlib\n", "# Compute the x and y coordinates for points on sine and cosine curves \n", "\n", "# Set up a subplot grid that has height 2 and width 1, \n", "# and set the first such subplot as active. \n", "\n", "# Make the first plot \n", " \n", "# Set the second subplot as active, and make the second plot. \n", "\n", "# Ensure tight layout\n", " \n", "# Show the figure. " ] } ], "metadata": { "kernelspec": { "display_name": "Python 3", "language": "python", "name": "python3" }, "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" } }, "nbformat": 4, "nbformat_minor": 2 } ================================================ FILE: intro_data_science/part_1/ds_part_1_complete.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "# A Brief Introduction to NumPy\n", "### \"...the fundamental package for scientific computing with Python.\" - numpy.org\n", "\n", "In this notebook, we will cover the basics of NumPy, a package that is the basis for many other libraries in the data science ecosystem. Let's get started." ] }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [], "source": [ "import numpy as np\n", "from IPython.display import Image\n", "import time\n", "from sys import getsizeof\n", "import matplotlib.pyplot as plt" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# 1. NumPy Arrays\n", "The array data structure is the backbone of the NumPy library. They can be single-dimensional (vectors), two-dimensional (matrices), or multi-dimensional for more complex tasks.\n", "\n", "In many ways, they are similar to Python lists." ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "a\n['b' 'c']\na\nb\nc\nd\ne\nf\n" ] } ], "source": [ "a = ['a', 'b', 'c', 'd', 'e', 'f']\n", "b = np.array(['a', 'b', 'c', 'd', 'e', 'f'])\n", "\n", "# Accessible by index\n", "print(b[0])\n", "\n", "# Sliceable\n", "print(b[1:3])\n", "\n", "# Iterable\n", "for letter in b:\n", " print(letter)" ] }, { "source": [ "So why use NumPy arrays at all? One word: performance! Generally speaking, Python lists take up more space and require more computation than NumPy arrays. Let's take a look at the size differences." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "8697456\n8000096\n" ] } ], "source": [ "n_elements = 1_000_000\n", "# Create using list comprehension\n", "python_list = [x for x in range(n_elements)]\n", "print(getsizeof(python_list))\n", "\n", "# Create with existing python list\n", "np_arr = np.array(python_list)\n", "print(getsizeof(np_arr))" ] }, { "source": [ "Now let's look at the speed differences." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 6, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "0.07268199999999991\n0.004588999999999954\n" ] } ], "source": [ "start = time.process_time()\n", "# Add 100 to every element in the Python list\n", "python_list_mod = [x + 100 for x in python_list]\n", "python_time = time.process_time() - start\n", "print(python_time)\n", "\n", "# Add 100 to every element in the Numpy array\n", "start = time.process_time()\n", "np_arr_mod = np_arr + 100\n", "np_time = time.process_time() - start\n", "print(np_time)" ] }, { "cell_type": "code", "execution_count": 7, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "image/png": 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"text/plain": [ "" ] }, "metadata": {}, "execution_count": 7 } ], "source": [ "Image('python_memory1.png')" ] }, { "source": [ "If NumPy arrays are more efficient computationally and in regards to space, why not use them all the time? There are some constraints, most notably, all of their items must be of the same type. [NumPy Array](https://numpy.org/doc/stable/reference/generated/numpy.array.html#numpy.array)" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 8, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "[1, 'a', 0.222, 'hello from inside the list!']\n['1' 'a' '0.222' 'hello from inside the list!']\n" ] } ], "source": [ "python_list = [1, 'a', 0.222, 'hello from inside the list!']\n", "np_arr = np.array(python_list)\n", "print(python_list)\n", "print(np_arr)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# 1.1 Creating\n", "NumPy arrays are created with existing data (standard python lists or lists of lists) or by using a collection of built-in methods." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.1 Existing Data" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .array()\n", "Use python lists (or lists of lists) as input." ] }, { "cell_type": "code", "execution_count": 2, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([1, 2, 3, 4, 5])" ] }, "metadata": {}, "execution_count": 2 } ], "source": [ "std_list = [1, 2, 3, 4, 5]\n", "np.array(std_list)" ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([[1, 2, 3],\n", " [4, 5, 6],\n", " [7, 8, 9]])" ] }, "metadata": {}, "execution_count": 3 } ], "source": [ "std_matrix = [[1, 2, 3], [4, 5, 6], [7, 8, 9]]\n", "np.array(std_matrix)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: the .array() method is a convenience function for constructing objects of the class ndarray. While it is possible to call .ndarray() directly, it is specifically regarded as an anti-pattern by the NumPy documentation." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.2 Fixed Values" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .zeros(), .ones()\n", "Return a new array of given shape and type, filled with zeros or ones." ] }, { "cell_type": "code", "execution_count": 4, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([0., 0., 0.])" ] }, "metadata": {}, "execution_count": 4 } ], "source": [ "np.zeros(3)" ] }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1., 1.])" ] }, "metadata": {}, "execution_count": 5 } ], "source": [ "np.ones(17)" ] }, { "cell_type": "code", "execution_count": 8, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([[0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.],\n", " [0., 0., 0., 0., 0.]])" ] }, "metadata": {}, "execution_count": 8 } ], "source": [ "# Notice the shape represented as a tuple\n", "np.zeros((5,5))" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: the numbers have periods after them to indicate that these are floating point numbers." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .full()\n", "Return a new array of given shape and type, filled with fill_value." ] }, { "cell_type": "code", "execution_count": 10, "metadata": { "scrolled": true }, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([[72, 72, 72, 72],\n", " [72, 72, 72, 72],\n", " [72, 72, 72, 72],\n", " [72, 72, 72, 72]])" ] }, "metadata": {}, "execution_count": 10 } ], "source": [ "np.full((4,4), 72)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.3 Range" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .arange()\n", "Return evenly spaced values within a given interval. Notice that the output is inclusive of the first number parameter and exclusive of the second." ] }, { "cell_type": "code", "execution_count": 10, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([0, 1, 2, 3, 4, 5, 6, 7, 8, 9])" ] }, "execution_count": 10, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.arange(0,10)" ] }, { "cell_type": "code", "execution_count": 11, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 2, 4, 6, 8])" ] }, "execution_count": 11, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.arange(0,10,2)" ] }, { "cell_type": "code", "execution_count": 12, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 5, 10, 15])" ] }, "execution_count": 12, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.arange(0,20,5)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .linspace()\n", "Return evenly spaced numbers over a specified interval. Notice that the output is inclusive of both the first and second number parameters." ] }, { "cell_type": "code", "execution_count": 13, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0., 5., 10., 15., 20.])" ] }, "execution_count": 13, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.linspace(0,20,5)" ] }, { "cell_type": "code", "execution_count": 14, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([0. , 0.05263158, 0.10526316, 0.15789474, 0.21052632,\n", " 0.26315789, 0.31578947, 0.36842105, 0.42105263, 0.47368421,\n", " 0.52631579, 0.57894737, 0.63157895, 0.68421053, 0.73684211,\n", " 0.78947368, 0.84210526, 0.89473684, 0.94736842, 1. ])" ] }, "execution_count": 14, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.linspace(0,1, 20)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: the main difference between .linspace() and .arange() is that with .linspace() you have precise control over the end value, whereas with .arange() you can specify the increments explicitly." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .logspace()\n", "Return numbers spaced evenly on a log scale." ] }, { "cell_type": "code", "execution_count": 15, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 100. , 215.443469 , 464.15888336, 1000. ])" ] }, "execution_count": 15, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.logspace(2.0, 3.0, num=4)" ] }, { "cell_type": "code", "execution_count": 25, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([4. , 5.0396842 , 6.34960421, 8. ])" ] }, "execution_count": 25, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.logspace(2.0, 3.0, num=4, base=2.0)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.1.4 Random" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .rand()\n", "Create an array of the given shape and populate it with random samples from a uniform distribution over 0,1." ] }, { "cell_type": "code", "execution_count": 27, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0.7125801811390514" ] }, "execution_count": 27, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.rand()" ] }, { "cell_type": "code", "execution_count": 18, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0.91155984, 0.98323076, 0.60797164, 0.48538736])" ] }, "execution_count": 18, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.rand(4)" ] }, { "cell_type": "code", "execution_count": 19, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[0.56443505, 0.13692564, 0.4367128 ],\n", " [0.66786468, 0.86782966, 0.88696338],\n", " [0.05537221, 0.54016645, 0.9654299 ],\n", " [0.19105141, 0.63037385, 0.51600478],\n", " [0.53737417, 0.76494641, 0.61205375],\n", " [0.09621612, 0.37848038, 0.60698676],\n", " [0.47772402, 0.61733058, 0.75352393]])" ] }, "execution_count": 19, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.rand(7,3)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .randn()\n", "Return a sample (or samples) from the “standard normal” distribution." ] }, { "cell_type": "code", "execution_count": 39, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0.36910179629879897" ] }, "execution_count": 39, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.randn()" ] }, { "cell_type": "code", "execution_count": 46, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([-0.08320502, -0.69825957, 0.80139519, 0.53540454, 0.78953503])" ] }, "execution_count": 46, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.randn(5)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: .rand() is from a uniform distribution, whereas .randn() is from the standard **normal** distribution." ] }, { "cell_type": "code", "execution_count": 55, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 1.33667905, 0.46529544, 1.51069451, 0.69167808, 1.60270943],\n", " [ 1.62614154, -0.10463841, -0.999014 , -0.70942201, -0.15136812],\n", " [-0.70328834, -0.48982338, 2.03118893, 2.32867839, 2.08885297]])" ] }, "execution_count": 55, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.randn(3, 5)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .randint()\n", "Return random integers from low (inclusive) to high (exclusive)." ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "5" ] }, "execution_count": 3, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.randint(0,10)" ] }, { "cell_type": "code", "execution_count": 66, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([4, 5, 5, 6, 1, 2, 1])" ] }, "execution_count": 66, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.randint(0,10,size=7)" ] }, { "cell_type": "code", "execution_count": 67, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[9, 3],\n", " [6, 8],\n", " [7, 6]])" ] }, "execution_count": 67, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.random.randint(0,10,size=(3,2))" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.2 Attributes and Methods" ] }, { "cell_type": "code", "execution_count": 82, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[2, 0, 8, 7, 8, 4, 6],\n", " [3, 5, 6, 6, 7, 6, 2]])" ] }, "execution_count": 82, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr = np.random.randint(0,10,size=(2,7))\n", "arr" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .shape\n", "Tuple of array dimensions. Note: this is an attribute NOT a method." ] }, { "cell_type": "code", "execution_count": 83, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "(2, 7)" ] }, "execution_count": 83, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.shape" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .reshape()\n", "Gives a new shape to an array without changing its data. Note: this happens 'in place' (does not return new values)." ] }, { "cell_type": "code", "execution_count": 88, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[2, 0],\n", " [8, 7],\n", " [8, 4],\n", " [6, 3],\n", " [5, 6],\n", " [6, 7],\n", " [6, 2]])" ] }, "execution_count": 88, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.reshape(7,2)" ] }, { "cell_type": "code", "execution_count": 85, "metadata": {}, "outputs": [ { "ename": "ValueError", "evalue": "cannot reshape array of size 14 into shape (12,4)", "output_type": "error", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mValueError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[0;32m----> 1\u001b[0;31m \u001b[0marr\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mreshape\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;36m12\u001b[0m\u001b[0;34m,\u001b[0m\u001b[0;36m4\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;31mValueError\u001b[0m: cannot reshape array of size 14 into shape (12,4)" ] } ], "source": [ "arr.reshape(12,4)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .newaxis\n", "Alternate syntax." ] }, { "cell_type": "code", "execution_count": 92, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[[2, 0, 8, 7, 8, 4, 6],\n", " [3, 5, 6, 6, 7, 6, 2]]])" ] }, "execution_count": 92, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr[np.newaxis, :]" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .dtype\n", "The type of data in the array." ] }, { "cell_type": "code", "execution_count": 80, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "dtype('int64')" ] }, "execution_count": 80, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.dtype" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .astype()\n", "Casts values to a specified type." ] }, { "cell_type": "code", "execution_count": 83, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[9, 5, 7, 3, 6, 8, 9],\n", " [9, 6, 1, 1, 4, 2, 3]], dtype=int8)" ] }, "execution_count": 83, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.astype('int8')" ] }, { "cell_type": "code", "execution_count": 81, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[9.+0.j, 5.+0.j, 7.+0.j, 3.+0.j, 6.+0.j, 8.+0.j, 9.+0.j],\n", " [9.+0.j, 6.+0.j, 1.+0.j, 1.+0.j, 4.+0.j, 2.+0.j, 3.+0.j]])" ] }, "execution_count": 81, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Complex numbers are the combination of a real and an imaginary number.\n", "arr.astype('complex')" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note: in mathematics i is used to denote imaginary numbers, but in Python (and many other languages) j is used because i tends to indicate the current value in a system. " ] }, { "cell_type": "code", "execution_count": 114, "metadata": {}, "outputs": [], "source": [ "arr2 = np.full((4,4), 199)" ] }, { "cell_type": "code", "execution_count": 115, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ True, True, True, True],\n", " [ True, True, True, True],\n", " [ True, True, True, True],\n", " [ True, True, True, True]])" ] }, "execution_count": 115, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr2.astype('bool')" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.3 Indexing" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 1.3.1 One-dimensional" ] }, { "cell_type": "code", "execution_count": 34, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20])" ] }, "execution_count": 34, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d = np.arange(0,21, 2)\n", "arr_1d" ] }, { "cell_type": "code", "execution_count": 35, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "10" ] }, "execution_count": 35, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the value at index 5 (the sixth element)\n", "arr_1d[5]" ] }, { "cell_type": "code", "execution_count": 36, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([2, 4, 6, 8])" ] }, "execution_count": 36, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get a slice of the array from index 1 (inclusive) to index 5 (exclusive)\n", "arr_1d[1:5]" ] }, { "cell_type": "code", "execution_count": 37, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 8, 10, 12, 14, 16, 18, 20])" ] }, "execution_count": 37, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get a slice of the array from index 4 to the end\n", "arr_1d[4:]" ] }, { "cell_type": "code", "execution_count": 38, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "20" ] }, "execution_count": 38, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the last element in the array\n", "arr_1d[-1]" ] }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [ { "ename": "NameError", "evalue": "name 'arr_1d' is not defined", "output_type": "error", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mNameError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Reverse the array\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0marr_1d\u001b[0m\u001b[0;34m[\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m-\u001b[0m\u001b[0;36m1\u001b[0m\u001b[0;34m]\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;31mNameError\u001b[0m: name 'arr_1d' is not defined" ] } ], "source": [ "# Reverse the array\n", "arr_1d[::-1]" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 1.3.2 Two-dimensional" ] }, { "cell_type": "code", "execution_count": 66, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 2, 3],\n", " [ 4, 5, 6, 7],\n", " [ 8, 9, 10, 11]])" ] }, "execution_count": 66, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_2d = np.arange(12).reshape((3, 4))\n", "arr_2d" ] }, { "cell_type": "code", "execution_count": 67, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 1, 2, 3])" ] }, "execution_count": 67, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the first row\n", "arr_2d[0]" ] }, { "cell_type": "code", "execution_count": 69, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "5" ] }, "execution_count": 69, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get the second element of the second row\n", "arr_2d[1][1]" ] }, { "cell_type": "code", "execution_count": 70, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "5" ] }, "execution_count": 70, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Alternative syntax\n", "arr_2d[1,1]" ] }, { "cell_type": "code", "execution_count": 71, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[0, 1, 2, 3],\n", " [4, 5, 6, 7]])" ] }, "execution_count": 71, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get first and second rows\n", "arr_2d[:2]" ] }, { "cell_type": "code", "execution_count": 72, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 4])" ] }, "execution_count": 72, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Get first element of both first and second rows\n", "arr_2d[:2,0]" ] }, { "cell_type": "code", "execution_count": 73, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[0],\n", " [4]])" ] }, "execution_count": 73, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Maintain shape\n", "arr_2d[:2,0:1]" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 1.3.3 Fancy Indexing" ] }, { "cell_type": "code", "execution_count": 86, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "[4, 6, 14, 16]" ] }, "execution_count": 86, "metadata": {}, "output_type": "execute_result" } ], "source": [ "[arr_1d[2], arr_1d[3], arr_1d[7], arr_1d[8]]" ] }, { "cell_type": "code", "execution_count": 87, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 4, 6, 14, 16])" ] }, "execution_count": 87, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d[[2,3,7,8]]" ] }, { "cell_type": "code", "execution_count": 88, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 4, 6],\n", " [14, 16]])" ] }, "execution_count": 88, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Output of fancy indexing\n", "ind = np.array([[2, 3],\n", " [7, 8]])\n", "arr_1d[ind]" ] }, { "cell_type": "code", "execution_count": 94, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([2, 7])" ] }, "execution_count": 94, "metadata": {}, "output_type": "execute_result" } ], "source": [ "row = [0, 1]\n", "col = [2, 3]\n", "arr_2d[(row, col)]" ] }, { "cell_type": "code", "execution_count": 96, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([2, 3])" ] }, "execution_count": 96, "metadata": {}, "output_type": "execute_result" } ], "source": [ "# Notice if second row value is not provided, NumPy compensates\n", "row = [0]\n", "col = [2, 3]\n", "arr_2d[(row, col)]" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 1.4 Selection" ] }, { "cell_type": "code", "execution_count": 20, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([1, 2, 3, 4])" ] }, "execution_count": 20, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr = np.arange(1,5)\n", "arr" ] }, { "cell_type": "code", "execution_count": 24, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([False, False, True, True])" ] }, "execution_count": 24, "metadata": {}, "output_type": "execute_result" } ], "source": [ "bool_arr = np.array([False, False, True, True])\n", "bool_arr" ] }, { "cell_type": "code", "execution_count": 25, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([3, 4])" ] }, "execution_count": 25, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr[bool_arr]" ] }, { "cell_type": "code", "execution_count": 32, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,\n", " 17, 18, 19])" ] }, "execution_count": 32, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_long = np.arange(0,20)\n", "arr_long" ] }, { "cell_type": "code", "execution_count": 34, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([12, 13, 14, 15, 16, 17, 18, 19])" ] }, "execution_count": 34, "metadata": {}, "output_type": "execute_result" } ], "source": [ "bool_arr_long = arr_long > 11\n", "arr_long[bool_arr_long]" ] }, { "cell_type": "code", "execution_count": 35, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 1, 2, 3, 4])" ] }, "execution_count": 35, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_long[arr_long < 5]" ] }, { "cell_type": "code", "execution_count": 36, "metadata": {}, "outputs": [], "source": [ "## TODO add exercise" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# 2. Operations\n", "One of the most powerful features of NumPy arrays is that operations are vectorized." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 2.1 Arithmetic\n", "Arithmetic operations work on NumPy arrays." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 2.1.1 One-dimensional" ] }, { "cell_type": "code", "execution_count": 59, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10])" ] }, "execution_count": 59, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d = np.arange(0,11)\n", "arr_1d" ] }, { "cell_type": "code", "execution_count": 63, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15])" ] }, "execution_count": 63, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d + 5" ] }, { "cell_type": "code", "execution_count": 105, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([-12, -11, -10, -9, -8, -7, -6, -5, -4, -3, -2])" ] }, "execution_count": 105, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d - 12" ] }, { "cell_type": "code", "execution_count": 64, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20])" ] }, "execution_count": 64, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d * 2" ] }, { "cell_type": "code", "execution_count": 101, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0. , 0.25, 0.5 , 0.75, 1. , 1.25, 1.5 , 1.75, 2. , 2.25, 2.5 ])" ] }, "execution_count": 101, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d / 4" ] }, { "cell_type": "code", "execution_count": 102, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([0, 0, 0, 0, 1, 1, 1, 1, 2, 2, 2])" ] }, "execution_count": 102, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d // 4" ] }, { "cell_type": "code", "execution_count": 103, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 0, 1, 8, 27, 64, 125, 216, 343, 512, 729, 1000])" ] }, "execution_count": 103, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_1d ** 3" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 2.1.2 Two-dimensional" ] }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 2, 3, 4],\n", " [ 5, 6, 7, 8, 9],\n", " [10, 11, 12, 13, 14]])" ] }, "execution_count": 5, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_2d = np.arange(15).reshape((3,5))\n", "arr_2d" ] }, { "cell_type": "code", "execution_count": 110, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 2, 4, 6, 8],\n", " [10, 12, 14, 16, 18],\n", " [20, 22, 24, 26, 28]])" ] }, "execution_count": 110, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_2d * 2" ] }, { "cell_type": "code", "execution_count": 111, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 4, 9, 16],\n", " [ 25, 36, 49, 64, 81],\n", " [100, 121, 144, 169, 196]])" ] }, "execution_count": 111, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_2d ** 2" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 2.1.3 Multiple values" ] }, { "cell_type": "code", "execution_count": 6, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([1, 2, 3, 4, 5])" ] }, "execution_count": 6, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_mult = np.array([1,2,3,4,5])\n", "arr_mult" ] }, { "cell_type": "code", "execution_count": 7, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 2, 6, 12, 20],\n", " [ 5, 12, 21, 32, 45],\n", " [10, 22, 36, 52, 70]])" ] }, "execution_count": 7, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_2d * arr_mult" ] }, { "cell_type": "code", "execution_count": 47, "metadata": {}, "outputs": [], "source": [ "arr_mult_2 = np.array([1,2,3])" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 2.2 Ufuncs\n", "Universal functions. For more information, visit: https://docs.scipy.org/doc/numpy/reference/ufuncs.html" ] }, { "cell_type": "code", "execution_count": 75, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 1, 2, 3, 4, 5, 6, 7, 8, 9, 10])" ] }, "execution_count": 75, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr = np.arange(1,11)\n", "arr" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .sum()\n", "Sum of array elements over a given axis." ] }, { "cell_type": "code", "execution_count": 105, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "70" ] }, "execution_count": 105, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.sum(arr)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .sqrt()\n", "Return the non-negative square-root of an array, element-wise." ] }, { "cell_type": "code", "execution_count": 76, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "3.0" ] }, "execution_count": 76, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.sqrt(9)" ] }, { "cell_type": "code", "execution_count": 77, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([1. , 1.41421356, 1.73205081, 2. , 2.23606798,\n", " 2.44948974, 2.64575131, 2.82842712, 3. , 3.16227766])" ] }, "execution_count": 77, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.sqrt(arr)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .power()\n", "First array elements raised to powers from second array, element-wise." ] }, { "cell_type": "code", "execution_count": 79, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "9" ] }, "execution_count": 79, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.power(3,2)" ] }, { "cell_type": "code", "execution_count": 81, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([ 1, 4, 9, 16, 25, 36, 49, 64, 81, 100])" ] }, "execution_count": 81, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.power(arr, 2)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### .min(), .max()" ] }, { "cell_type": "code", "execution_count": 65, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([3, 5, 4, 0, 3, 5, 5, 9, 7, 7])" ] }, "execution_count": 65, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr = np.random.randint(0,10, 10)\n", "arr" ] }, { "cell_type": "code", "execution_count": 67, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0" ] }, "execution_count": 67, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.min(arr)" ] }, { "cell_type": "code", "execution_count": 68, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "0" ] }, "execution_count": 68, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.min()" ] }, { "cell_type": "code", "execution_count": 69, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "9" ] }, "execution_count": 69, "metadata": {}, "output_type": "execute_result" } ], "source": [ "np.max(arr)" ] }, { "cell_type": "code", "execution_count": 70, "metadata": { "scrolled": true }, "outputs": [ { "data": { "text/plain": [ "9" ] }, "execution_count": 70, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr.max()" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 2.3 Broadcasting\n", "The term broadcasting describes how numpy treats arrays with different shapes during arithmetic operations. Subject to certain constraints, the smaller array is “broadcast” across the larger array so that they have compatible shapes. (NumPy documentation) " ] }, { "cell_type": "code", "execution_count": 93, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[ 0, 1, 2, 3, 4],\n", " [ 5, 6, 7, 8, 9],\n", " [10, 11, 12, 13, 14],\n", " [15, 16, 17, 18, 19]])" ] }, "execution_count": 93, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one = np.arange(0,20).reshape(4,5)\n", "arr_one" ] }, { "cell_type": "code", "execution_count": 94, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 11, 12, 13, 14],\n", " [15, 16, 17, 18, 19],\n", " [20, 21, 22, 23, 24],\n", " [25, 26, 27, 28, 29]])" ] }, "execution_count": 94, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + 10" ] }, { "cell_type": "code", "execution_count": 96, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 11, 12, 13, 14],\n", " [15, 16, 17, 18, 19],\n", " [20, 21, 22, 23, 24],\n", " [25, 26, 27, 28, 29]])" ] }, "execution_count": 96, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + np.array([10])" ] }, { "cell_type": "code", "execution_count": 97, "metadata": {}, "outputs": [ { "ename": "ValueError", "evalue": "operands could not be broadcast together with shapes (4,5) (2,) ", "output_type": "error", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mValueError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[0;32m----> 1\u001b[0;31m \u001b[0marr_one\u001b[0m \u001b[0;34m+\u001b[0m \u001b[0mnp\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0marray\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m[\u001b[0m\u001b[0;36m10\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0;36m20\u001b[0m\u001b[0;34m]\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;31mValueError\u001b[0m: operands could not be broadcast together with shapes (4,5) (2,) " ] } ], "source": [ "arr_one + np.array([10, 20])" ] }, { "cell_type": "code", "execution_count": 99, "metadata": {}, "outputs": [], "source": [ "arr_two = np.array([10,20,30,40,50])" ] }, { "cell_type": "code", "execution_count": 100, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 21, 32, 43, 54],\n", " [15, 26, 37, 48, 59],\n", " [20, 31, 42, 53, 64],\n", " [25, 36, 47, 58, 69]])" ] }, "execution_count": 100, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + arr_two" ] }, { "cell_type": "code", "execution_count": 101, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "(4, 5)" ] }, "execution_count": 101, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one.shape" ] }, { "cell_type": "code", "execution_count": 102, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "(5,)" ] }, "execution_count": 102, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_two.shape" ] }, { "cell_type": "code", "execution_count": 103, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10],\n", " [20],\n", " [30],\n", " [40],\n", " [50]])" ] }, "execution_count": 103, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_two.reshape(5,1)" ] }, { "cell_type": "code", "execution_count": 104, "metadata": {}, "outputs": [ { "data": { "text/plain": [ "array([[10, 21, 32, 43, 54],\n", " [15, 26, 37, 48, 59],\n", " [20, 31, 42, 53, 64],\n", " [25, 36, 47, 58, 69]])" ] }, "execution_count": 104, "metadata": {}, "output_type": "execute_result" } ], "source": [ "arr_one + arr_two" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Array comparison begins with the trailing dimensions and subsequently works its way foward. Two array dimensions are compatible when:\n", "- they are equal, or\n", "- one of them is 1\n", "(NumPy documentation)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "https://jakevdp.github.io/PythonDataScienceHandbook/02.05-computation-on-arrays-broadcasting.html" ] }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "output_type": "display_data", "data": { "text/plain": "
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\n" }, "metadata": { "needs_background": "light" } } ], "source": [ "# Plot sin and cos on the same graph, using matplotlib\n", "# Compute the x and y coordinates for points on sine and cosine curves \n", "x = np.arange(0, 20 * np.pi, 0.2) \n", "\n", "y_sin = np.sin(x) \n", "y_cos = np.cos(x) \n", " \n", "# Set up a subplot grid that has height 2 and width 1, \n", "# and set the first such subplot as active. \n", "plt.subplot(2, 1, 1)\n", " \n", "# Make the first plot \n", "plt.plot(x, y_sin) \n", "plt.title('Sine') \n", " \n", "# Set the second subplot as active, and make the second plot. \n", "plt.subplot(2, 1, 2) \n", "plt.plot(x, y_cos) \n", "plt.title('Cosine') \n", "\n", "# Ensure tight layout\n", "plt.tight_layout()\n", " \n", "# Show the figure. \n", "plt.show()" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] } ], "metadata": { "kernelspec": { "display_name": "Python 3", "language": "python", "name": "python3" }, "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" } }, "nbformat": 4, "nbformat_minor": 2 } ================================================ FILE: intro_data_science/part_2/binder/requirements.txt ================================================ pandas==1.2.0 matplotlib==3.3.3 numpy==1.19.5 ================================================ FILE: intro_data_science/part_2/ds_part_2.ipynb ================================================ { "metadata": { "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" }, "orig_nbformat": 2, "kernelspec": { "name": "python3", "display_name": "Python 3", "language": "python" } }, "nbformat": 4, "nbformat_minor": 2, "cells": [ { "source": [ "# A Brief Introduction to Matplotlib\n", "\n", "In this notebook, we will cover the basics of Matplotlib, a powerful, prolific library \n", "for organizing and visualizing data of all kinds. If you work in science, chances are you'll\n", "find a use case for this. Enjoy!" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "import matplotlib.pyplot as plt\n", "import numpy as np" ] }, { "source": [ "Now, let's make our first plot - a line graph! We're going to visualize data related to CO2 emissions." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Use numpy to generate range of years to cover\n", "\n", "# Emissions data for China\n", "china_emissions = [8500.543, 9388.199, 9633.899, 9796.527, 9820.36, 9716.468, 9704.479, 9838.754, 10064.686]\n", "\n", "# Emissions data for USA\n", "us_emissions = [5700.108, 5572.585, 5371.777, 5522.908, 5572.106, 5422.966, 5306.662, 5270.749, 5416.278]\n", "\n", "# Emissions data for India\n", "india_emissions = [1700.027, 1811.961, 1979.047, 1994.101, 2199.4, 2298.17, 2371.752, 2456.954, 2654.101]\n", "\n", "# Emissions data for Russia\n", "russia_emissions = [1613.523, 1613.523, 1679.385, 1618.434, 1617.678, 1622.498, 1617.653, 1647.041, 1710.688]\n", "\n", "# Add style to the x ticks\n", "\n", "# Add labels\n", "\n", "# Add a legend\n", "\n", "# Ensure a tight, uncluttered layout\n", "\n", "# Save the figure as png, jpg, and svg\n", "\n", "# Annotate with source\n", "\n", "# Set the plot style\n", "\n", "# Show the figure" ] }, { "source": [ "Looks pretty good! But it's hard to see the relationship between Russia and the other countries. Let's transform this into a bar graph." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Simple bar graph" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Stacked bar graph" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Grouped bar graph" ] }, { "source": [ "Great! Now let's explore this data as a percentage of the total global emissions." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Total global emissions for those years\n", "world_emissions = [33066.651, 34357.366, 34919.289, 35207.886, 35505.827, 35462.747, 35675.099, 36153.262, 36572.754]" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Plot multiple years on the same figure" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] } ] } ================================================ FILE: intro_data_science/part_2/ds_part_2_complete.ipynb ================================================ { "metadata": { "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" }, "orig_nbformat": 2, "kernelspec": { "name": "python3", "display_name": "Python 3", "language": "python" } }, "nbformat": 4, "nbformat_minor": 2, "cells": [ { "source": [ "# A Brief Introduction to Matplotlib\n", "\n", "In this notebook, we will cover the basics of Matplotlib, a powerful, prolific library \n", "for organizing and visualizing data of all kinds. If you work in science, chances are you'll\n", "find a use case for this. Enjoy!" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 226, "metadata": {}, "outputs": [], "source": [ "import matplotlib.pyplot as plt\n", "import numpy as np" ] }, { "source": [ "Now, let's make our first plot - a line graph! We're going to visualize data related to CO2 emissions." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 191, "metadata": {}, "outputs": [ { "output_type": "display_data", "data": { "text/plain": "
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\n" }, "metadata": {} } ], "source": [ "# Use numpy to generate range of years to cover\n", "years = np.arange(2010, 2019)\n", "\n", "# Emissions data for China\n", "china_emissions = [8500.543, 9388.199, 9633.899, 9796.527, 9820.36, 9716.468, 9704.479, 9838.754, 10064.686]\n", "plt.plot(years, china_emissions, label='China', color='red')\n", "\n", "# Emissions data for USA\n", "us_emissions = [5700.108, 5572.585, 5371.777, 5522.908, 5572.106, 5422.966, 5306.662, 5270.749, 5416.278]\n", "plt.plot(years, us_emissions, label='USA', color='blue')\n", "\n", "# Emissions data for India\n", "india_emissions = [1700.027, 1811.961, 1979.047, 1994.101, 2199.4, 2298.17, 2371.752, 2456.954, 2654.101]\n", "plt.plot(years, india_emissions, marker=\"o\", label='India', color='#228B22')\n", "\n", "russia_emissions = [1613.523, 1613.523, 1679.385, 1618.434, 1617.678, 1622.498, 1617.653, 1647.041, 1710.688]\n", "plt.plot(years, russia_emissions, label='Russia', color='purple')\n", "\n", "# Add style to the x ticks\n", "plt.xticks(ticks=years, labels=years, rotation=45)\n", "\n", "# Change scaling for y axis\n", "# plt.axis([2010, 2018, 0, 30])\n", "\n", "# Add labels\n", "plt.xlabel('Year')\n", "plt.ylabel('CO2')\n", "plt.title('CO2 Emissions')\n", "\n", "# Add a legend\n", "plt.legend(bbox_to_anchor=(1, 1), prop={'size': 12})\n", "\n", "# Ensure a tight, uncluttered layout\n", "# plt.tight_layout()\n", "\n", "# Save the figure\n", "plt.savefig('./out/co2_plot1.png')\n", "plt.savefig('./out/co2_plot2.jpg')\n", "plt.savefig('./out/co2_plot3.svg')\n", "\n", "# Annotate with source\n", "# Placement of annotation is fraction of the axes coordinate system\n", "# Placement of text is an offset in points\n", "plt.annotate('OWID: https://cdiac.ess-dive.lbl.gov/', (0,0), (-65,-65), fontsize=10, \n", " xycoords='axes fraction', textcoords='offset points')\n", "\n", "# Set the plot style\n", "plt.style.use('fivethirtyeight')\n", "\n", "# Show the figure\n", "plt.show()" ] }, { "source": [ "Looks pretty good! But it's hard to see the relationship between Russia and the other countries. Let's transform this into a bar graph." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 229, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "Text(0.5, 1.0, 'CO2 Emissions - 2010')" ] }, "metadata": {}, "execution_count": 229 }, { "output_type": "display_data", "data": { "text/plain": "
", "image/svg+xml": "\n\n\n\n \n \n \n \n 2021-01-25T12:30:41.408505\n image/svg+xml\n \n \n Matplotlib v3.3.3, https://matplotlib.org/\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n", "image/png": 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\n" }, "metadata": {} } ], "source": [ "emissions_arr = [china_emissions[0], us_emissions[0], india_emissions[0], russia_emissions[0]]\n", "countries = ['China', 'USA', 'India', 'Russia']\n", "# plt.bar(countries, emissions_arr)\n", "plt.barh(countries, emissions_arr)\n", "plt.title('CO2 Emissions - 2010')" ] }, { "cell_type": "code", "execution_count": 230, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "Text(0.5, 1.0, 'CO2 Emissions by Year')" ] }, "metadata": {}, "execution_count": 230 }, { "output_type": "display_data", "data": { "text/plain": "
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\n" }, "metadata": {} } ], "source": [ "# Stacked bar chart\n", "plt.bar(years, china_emissions)\n", "plt.bar(years, us_emissions)\n", "plt.bar(years, india_emissions)\n", "plt.bar(years, russia_emissions)\n", "plt.legend(['China', 'USA', 'India', 'Russia'], bbox_to_anchor=(1, 1))\n", "plt.title('CO2 Emissions by Year')" ] }, { "cell_type": "code", "execution_count": 231, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "Text(0.5, 1.0, 'CO2 Emissions by Year')" ] }, "metadata": {}, "execution_count": 231 }, { "output_type": "display_data", "data": { "text/plain": "
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\n" }, "metadata": {} } ], "source": [ "# Grouped bar chart\n", "width = 0.2\n", "plt.bar(years - (width * 2), china_emissions, width=width)\n", "plt.bar(years - width, us_emissions, width=width)\n", "plt.bar(years, india_emissions, width=width)\n", "plt.bar(years + width, russia_emissions, width=width)\n", "plt.legend(['China', 'USA', 'India', 'Russia'], bbox_to_anchor=(1, 1))\n", "plt.title('CO2 Emissions by Year')" ] }, { "source": [ "Great! Now let's explore this data as a percentage of the total global emissions." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 232, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "Text(-65, -65, 'OWID: https://cdiac.ess-dive.lbl.gov/')" ] }, "metadata": {}, "execution_count": 232 }, { "output_type": "display_data", "data": { "text/plain": "
", "image/svg+xml": "\n\n\n\n \n \n \n \n 2021-01-25T12:30:56.468951\n image/svg+xml\n \n \n Matplotlib v3.3.3, https://matplotlib.org/\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n", "image/png": 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\n" }, "metadata": {} } ], "source": [ "# Total global emissions for those years\n", "world_emissions = [33066.651, 34357.366, 34919.289, 35207.886, 35505.827, 35462.747, 35675.099, 36153.262, 36572.754]\n", "\n", "year_idx = 0\n", "\n", "# Get percentage of total emissions contributed by China for 2010\n", "china_prct = (china_emissions[year_idx] / world_emissions[year_idx]) * 100\n", "us_prct = (us_emissions[year_idx] / world_emissions[year_idx]) * 100\n", "india_prct = (india_emissions[year_idx] / world_emissions[year_idx]) * 100\n", "russia_prct = (russia_emissions[year_idx] / world_emissions[year_idx]) * 100\n", "world_prct = 100\n", "other_prct = (world_prct - (china_prct + us_prct + india_prct + russia_prct)) / world_prct * 100\n", "\n", "percentages = [china_prct, us_prct, india_prct, russia_prct, other_prct]\n", "\n", "plt.pie(\n", " x=percentages, \n", " shadow=True, \n", " labels=['China', 'USA', 'India', 'Russia', 'Rest of World'], \n", " autopct='%1.1f%%',\n", " # explode=[0,0.1,0.2,0.3,0.1],\n", " # startangle=90\n", " )\n", "\n", "plt.title('CO2 Emissions')\n", "plt.annotate('OWID: https://cdiac.ess-dive.lbl.gov/', (0,0), (-65,-65), fontsize=10, \n", " xycoords='axes fraction', textcoords='offset points')" ] }, { "cell_type": "code", "execution_count": 233, "metadata": {}, "outputs": [ { "output_type": "display_data", "data": { "text/plain": "
", "image/svg+xml": "\n\n\n\n \n \n \n \n 2021-01-25T12:30:59.092616\n image/svg+xml\n \n \n Matplotlib v3.3.3, https://matplotlib.org/\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n 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\n" }, "metadata": {} } ], "source": [ "year_idx = 0\n", "fig, axes = plt.subplots(nrows=4, ncols=2)\n", "for row in axes:\n", " for col in row:\n", " china_prct = (china_emissions[year_idx] / world_emissions[year_idx]) * 100\n", " us_prct = (us_emissions[year_idx] / world_emissions[year_idx]) * 100\n", " india_prct = (india_emissions[year_idx] / world_emissions[year_idx]) * 100\n", " russia_prct = (russia_emissions[year_idx] / world_emissions[year_idx]) * 100\n", " world_prct = 100\n", " other_prct = (world_prct - (china_prct + us_prct + india_prct + russia_prct)) / world_prct * 100\n", " percentages = [china_prct, us_prct, india_prct, russia_prct, other_prct]\n", "\n", " col.pie(\n", " x=percentages, \n", " shadow=True, \n", " labels=['China', 'USA', 'India', 'Russia', 'Rest of World'], \n", " autopct='%1.1f%%',\n", " # explode=[0,0.1,0.2,0.3,0.1],\n", " # startangle=90\n", " )\n", " col.set_xlabel(f'Year: {years[year_idx]}')\n", " year_idx = year_idx + 1\n", "\n", "fig.set_size_inches(10, 15)\n", "fig.suptitle('Top CO2 Emitters by Percentage')\n", "fig.tight_layout()\n", "fig.show()" ] } ] } ================================================ FILE: intro_data_science/part_3/binder/requirements.txt ================================================ pandas==1.2.0 matplotlib==3.3.3 numpy==1.19.5 openpyxl==3.0.6 ================================================ FILE: intro_data_science/part_3/data/complete/eur_data_final.csv ================================================ ,country,unemp_rate,gdp,median_income,total_pop 0,Austria,6.0,356237.6,23071,8401940 1,Belgium,7.8,424660.3,21335,11000638 2,Bulgaria,7.6,48128.6,6742,7364570 3,Croatia,13.1,46639.5,8985,4284889 4,Cyprus,13.0,18490.2,16173,840407 5,Czechia,4.0,176370.1,12478,10436560 6,Denmark,6.2,282089.9,21355,5560628 7,Estonia,6.8,21682.6,11867,1294455 8,Finland,8.8,216073.0,19997,5375276 9,France,10.1,2228568.0,20621,64933400 10,Germany,4.1,3159750.0,21152,80219695 11,Greece,23.6,176487.9,9048,10816286 12,Hungary,5.1,113903.8,8267,9937628 13,Iceland,3.0,18646.1,22193,315556 14,Ireland,8.4,273238.2,18286,4574888 15,Italy,11.7,1689824.0,16237,59433744 16,Latvia,9.6,25037.7,9257,2070371 17,Lithuania,7.9,38849.4,9364,3043429 18,Luxembourg,6.3,53303.0,28663,512353 19,Malta,4.7,10344.1,17264,417432 20,Netherlands,6.0,708337.0,21189,16655799 21,Norway,4.7,335747.5,27670,4979954 22,Poland,6.2,426547.5,10865,38044565 23,Portugal,11.2,186480.5,10805,10562178 24,Romania,5.9,170393.6,4724,20121641 25,Slovakia,9.7,81226.1,10466,5397036 26,Slovenia,8.0,40357.2,15250,2050189 27,Spain,19.6,1118743.0,15347,46815910 28,Sweden,7.0,463147.5,20955,9482855 29,Switzerland,5.0,605753.7,27692,7954662 30,Turkey,10.9,780224.9,6501,7954662 31,United Kingdom,4.8,2403382.6,17296,63182180 ================================================ FILE: intro_data_science/part_3/data/gdp_2016.csv ================================================ country,gdp Cyprus,18490.2 Latvia,25037.7 Lithuania,38849.4 Luxembourg,53303 Hungary,113903.8 Malta,10344.1 Netherlands,708337 Austria,356237.6 Poland,426547.5 Portugal,186480.5 Romania,170393.6 Slovenia,40357.2 Slovakia,81226.1 Finland,216073 Sweden,463147.5 United Kingdom,2403382.6 Iceland,18646.1 Norway,335747.5 Switzerland,605753.7 Turkey,780224.9 Belgium,424660.3 Bulgaria,48128.6 Czechia,176370.1 Denmark,282089.9 Germany,3159750 Estonia,21682.6 Ireland,273238.2 Greece,176487.9 Spain,1118743 France,2228568 Croatia,46639.5 Italy,1689824 ================================================ FILE: intro_data_science/part_3/data/out/eur_data_sorted.csv ================================================ ,country,unemp_rate,gdp,total_pop 0,Austria,6.0,356237.6,8401940 1,Belgium,7.8,424660.3,11000638 2,Bulgaria,7.6,48128.6,7364570 13,Croatia,13.1,46639.5,4284889 4,Cyprus,13.0,18490.2,840407 5,Czechia,4.0,176370.1,10436560 7,Denmark,6.2,282089.9,5560628 8,Estonia,6.8,21682.6,1294455 11,Finland,8.8,216073.0,5375276 12,France,10.1,2228568.0,64933400 6,Germany,4.1,3159750.0,80219695 9,Greece,23.6,176487.9,10816286 14,Hungary,5.1,113903.8,9937628 16,Iceland,3.0,18646.1,315556 15,Ireland,8.4,273238.2,4574888 17,Italy,11.7,1689824.0,59433744 20,Latvia,9.6,25037.7,2070371 18,Lithuania,7.9,38849.4,3043429 19,Luxembourg,6.3,53303.0,512353 21,Malta,4.7,10344.1,417432 22,Netherlands,6.0,708337.0,16655799 23,Norway,4.7,335747.5,4979954 24,Poland,6.2,426547.5,38044565 25,Portugal,11.2,186480.5,10562178 26,Romania,5.9,170393.6,20121641 29,Slovakia,9.7,81226.1,5397036 28,Slovenia,8.0,40357.2,2050189 10,Spain,19.6,1118743.0,46815910 27,Sweden,7.0,463147.5,9482855 3,Switzerland,5.0,605753.7,7954662 30,Turkey,10.9,780224.9,7954662 31,United Kingdom,4.8,2403382.6,63182180 ================================================ FILE: intro_data_science/part_3/data/out/eur_data_sorted.html ================================================
country unemp_rate gdp total_pop
0 Austria 6.0 356237.6 8401940
1 Belgium 7.8 424660.3 11000638
2 Bulgaria 7.6 48128.6 7364570
13 Croatia 13.1 46639.5 4284889
4 Cyprus 13.0 18490.2 840407
5 Czechia 4.0 176370.1 10436560
7 Denmark 6.2 282089.9 5560628
8 Estonia 6.8 21682.6 1294455
11 Finland 8.8 216073.0 5375276
12 France 10.1 2228568.0 64933400
6 Germany 4.1 3159750.0 80219695
9 Greece 23.6 176487.9 10816286
14 Hungary 5.1 113903.8 9937628
16 Iceland 3.0 18646.1 315556
15 Ireland 8.4 273238.2 4574888
17 Italy 11.7 1689824.0 59433744
20 Latvia 9.6 25037.7 2070371
18 Lithuania 7.9 38849.4 3043429
19 Luxembourg 6.3 53303.0 512353
21 Malta 4.7 10344.1 417432
22 Netherlands 6.0 708337.0 16655799
23 Norway 4.7 335747.5 4979954
24 Poland 6.2 426547.5 38044565
25 Portugal 11.2 186480.5 10562178
26 Romania 5.9 170393.6 20121641
29 Slovakia 9.7 81226.1 5397036
28 Slovenia 8.0 40357.2 2050189
10 Spain 19.6 1118743.0 46815910
27 Sweden 7.0 463147.5 9482855
3 Switzerland 5.0 605753.7 7954662
30 Turkey 10.9 780224.9 7954662
31 United Kingdom 4.8 2403382.6 63182180
================================================ FILE: intro_data_science/part_3/data/out/eur_data_sorted.json ================================================ {"country":{"0":"Austria","1":"Belgium","2":"Bulgaria","13":"Croatia","4":"Cyprus","5":"Czechia","7":"Denmark","8":"Estonia","11":"Finland","12":"France","6":"Germany","9":"Greece","14":"Hungary","16":"Iceland","15":"Ireland","17":"Italy","20":"Latvia","18":"Lithuania","19":"Luxembourg","21":"Malta","22":"Netherlands","23":"Norway","24":"Poland","25":"Portugal","26":"Romania","29":"Slovakia","28":"Slovenia","10":"Spain","27":"Sweden","3":"Switzerland","30":"Turkey","31":"United Kingdom"},"unemp_rate":{"0":6.0,"1":7.8,"2":7.6,"13":13.1,"4":13.0,"5":4.0,"7":6.2,"8":6.8,"11":8.8,"12":10.1,"6":4.1,"9":23.6,"14":5.1,"16":3.0,"15":8.4,"17":11.7,"20":9.6,"18":7.9,"19":6.3,"21":4.7,"22":6.0,"23":4.7,"24":6.2,"25":11.2,"26":5.9,"29":9.7,"28":8.0,"10":19.6,"27":7.0,"3":5.0,"30":10.9,"31":4.8},"gdp":{"0":356237.6,"1":424660.3,"2":48128.6,"13":46639.5,"4":18490.2,"5":176370.1,"7":282089.9,"8":21682.6,"11":216073.0,"12":2228568.0,"6":3159750.0,"9":176487.9,"14":113903.8,"16":18646.1,"15":273238.2,"17":1689824.0,"20":25037.7,"18":38849.4,"19":53303.0,"21":10344.1,"22":708337.0,"23":335747.5,"24":426547.5,"25":186480.5,"26":170393.6,"29":81226.1,"28":40357.2,"10":1118743.0,"27":463147.5,"3":605753.7,"30":780224.9,"31":2403382.6000000001},"total_pop":{"0":8401940,"1":11000638,"2":7364570,"13":4284889,"4":840407,"5":10436560,"7":5560628,"8":1294455,"11":5375276,"12":64933400,"6":80219695,"9":10816286,"14":9937628,"16":315556,"15":4574888,"17":59433744,"20":2070371,"18":3043429,"19":512353,"21":417432,"22":16655799,"23":4979954,"24":38044565,"25":10562178,"26":20121641,"29":5397036,"28":2050189,"10":46815910,"27":9482855,"3":7954662,"30":7954662,"31":63182180}} ================================================ FILE: intro_data_science/part_3/data/unemployment_2016.csv ================================================ country,unemp_rate Austria,6 Belgium,7.8 Bulgaria,7.6 Switzerland,5 Cyprus,13 Czechia,4 Germany,4.1 Denmark,6.2 Estonia,6.8 Greece,23.6 Spain,19.6 Finland,8.8 France,10.1 Croatia,13.1 Hungary,5.1 Ireland,8.4 Iceland,3 Italy,11.7 Lithuania,7.9 Luxembourg,6.3 Latvia,9.6 Malta,4.7 Netherlands,6 Norway,4.7 Poland,6.2 Portugal,11.2 Romania,5.9 Sweden,7 Slovenia,8 Slovakia,9.7 Turkey,10.9 United Kingdom,4.8 ================================================ FILE: intro_data_science/part_3/ds_part_3.ipynb ================================================ { "metadata": { "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" }, "orig_nbformat": 2, "kernelspec": { "name": "python3", "display_name": "Python 3", "language": "python" } }, "nbformat": 4, "nbformat_minor": 2, "cells": [ { "source": [ "# A Brief Introduction to Pandas\n", "### Part 1\n", "\n", "In this notebook, we will cover the basics of Pandas, a dynamic, powerful Python library that is ubiquitous in the field of Data Science. It has robust I/O functionality and makes working with data a lot simpler than doing so in Excel. Let's get stared." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 198, "metadata": {}, "outputs": [], "source": [ "import pandas as pd\n", "import matplotlib.pyplot as plt" ] }, { "source": [ "## 1.1 DataFrames\n", "Let's read in some existing data using Pandas convenient read_csv method." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 94, "metadata": {}, "outputs": [], "source": [ "unemployment_df = pd.read_csv('./data/unemployment_2016.csv')" ] }, { "cell_type": "code", "execution_count": 95, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "pandas.core.frame.DataFrame" ] }, "metadata": {}, "execution_count": 95 } ], "source": [ "# Check the type\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.html" ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [], "source": [ "# Use .info() and .describe to inspect dataframe" ] }, { "cell_type": "code", "execution_count": 98, "metadata": {}, "outputs": [], "source": [ "# Select a single column\n", "# Using bracket notation\n", "\n", "# Using dot notation" ] }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [], "source": [ "# Access by numeric index" ] }, { "cell_type": "code", "execution_count": 2, "metadata": {}, "outputs": [], "source": [ "# Check the type\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.Series.html" ] }, { "cell_type": "code", "execution_count": 4, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "NameError", "evalue": "name 'countries' is not defined", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mNameError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Series objects do not have the same methods as dataframes\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0mcountries\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0minfo\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 3\u001b[0m \u001b[0mcountries\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mdescribe\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mNameError\u001b[0m: name 'countries' is not defined" ] } ], "source": [ "# Series objects do not have the same methods as dataframes\n", "countries.info()\n", "countries.describe()" ] }, { "source": [ "## 1.2 Vectorized Operations\n", "In Pandas, operations work on each value in most data structures, such as dataframes and series." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [], "source": [ "# Add a string to the countries series" ] }, { "cell_type": "code", "execution_count": 103, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "TypeError", "evalue": "can only concatenate str (not \"int\") to str", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mTypeError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/ops/array_ops.py\u001b[0m in \u001b[0;36m_na_arithmetic_op\u001b[0;34m(left, right, op, is_cmp)\u001b[0m\n\u001b[1;32m 141\u001b[0m \u001b[0;32mtry\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m--> 142\u001b[0;31m \u001b[0mresult\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0mexpressions\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mevaluate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mop\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mleft\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mright\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 143\u001b[0m \u001b[0;32mexcept\u001b[0m \u001b[0mTypeError\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/computation/expressions.py\u001b[0m in \u001b[0;36mevaluate\u001b[0;34m(op, a, b, use_numexpr)\u001b[0m\n\u001b[1;32m 234\u001b[0m \u001b[0;31m# error: \"None\" not callable\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m--> 235\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0m_evaluate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mop\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mop_str\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0ma\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mb\u001b[0m\u001b[0;34m)\u001b[0m \u001b[0;31m# type: ignore[misc]\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 236\u001b[0m \u001b[0;32mreturn\u001b[0m \u001b[0m_evaluate_standard\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mop\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mop_str\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0ma\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mb\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/computation/expressions.py\u001b[0m in \u001b[0;36m_evaluate_standard\u001b[0;34m(op, op_str, a, b)\u001b[0m\n\u001b[1;32m 68\u001b[0m \u001b[0;32mwith\u001b[0m \u001b[0mnp\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0merrstate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mall\u001b[0m\u001b[0;34m=\u001b[0m\u001b[0;34m\"ignore\"\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m---> 69\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0mop\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0ma\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mb\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 70\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mTypeError\u001b[0m: can only concatenate str (not \"int\") to str", "\nDuring handling of the above exception, another exception occurred:\n", "\u001b[0;31mTypeError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Add a number to the countries series\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0mcountries\u001b[0m \u001b[0;34m+\u001b[0m \u001b[0;36m12\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/ops/common.py\u001b[0m in \u001b[0;36mnew_method\u001b[0;34m(self, other)\u001b[0m\n\u001b[1;32m 63\u001b[0m \u001b[0mother\u001b[0m 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\u001b[0;34m@\u001b[0m\u001b[0munpack_zerodim_and_defer\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m\"__add__\"\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 88\u001b[0m \u001b[0;32mdef\u001b[0m \u001b[0m__add__\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mself\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mother\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m---> 89\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m_arith_method\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mother\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0moperator\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0madd\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 90\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 91\u001b[0m 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"\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/ops/array_ops.py\u001b[0m in \u001b[0;36marithmetic_op\u001b[0;34m(left, right, op)\u001b[0m\n\u001b[1;32m 187\u001b[0m \u001b[0;32melse\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 188\u001b[0m \u001b[0;32mwith\u001b[0m \u001b[0mnp\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0merrstate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mall\u001b[0m\u001b[0;34m=\u001b[0m\u001b[0;34m\"ignore\"\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m--> 189\u001b[0;31m \u001b[0mres_values\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0m_na_arithmetic_op\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mlvalues\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mrvalues\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mop\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 190\u001b[0m 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\u001b[0;34m~\u001b[0m\u001b[0mmask\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mnp\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mnan\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mTypeError\u001b[0m: can only concatenate str (not \"int\") to str" ] } ], "source": [ "# Add a number to the countries series\n", "countries + 12" ] }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [], "source": [ "# Select the unemployment rates as a series" ] }, { "cell_type": "code", "execution_count": 2, "metadata": {}, "outputs": [], "source": [ "# Sort the dataframe by rate" ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [], "source": [ "# Use .head() to get the top five values" ] }, { "cell_type": "code", "execution_count": 4, "metadata": {}, "outputs": [], "source": [ "# Use .tail() to get the five values" ] }, { "source": [ "### Exercise - What is the average unemployment of the seven countries with the highest unemployment in europe?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [], "source": [ "# Sort the values of the unemployment_df - descending or ascending?\n", "\n", "# Get the first seven rows in the returned series\n", "\n", "# Get the average of those rows" ] }, { "source": [ "### Extra - explore these methods:\n", ".min(), .max(), .sum(), .unique(), .nunique(), .count(), .duplicated()" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 9, "metadata": {}, "outputs": [], "source": [ "# " ] }, { "source": [ "## 1.3 Merging DataFrames\n", "Data can come from a variety of different sources and it can be helpful to put them all in one place." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 7, "metadata": {}, "outputs": [], "source": [ "gdp_filepath = './data/gdp_2016.csv'\n", "# Read in data using this filepath" ] }, { "cell_type": "code", "execution_count": 8, "metadata": {}, "outputs": [], "source": [ "# Inspect the GDP data" ] }, { "source": [ "Let's create a new dataframe by merging two existing dataframes. This is the one we'll use for the rest of section 2." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 10, "metadata": {}, "outputs": [], "source": [ "# Merge unemployment df with gdp df\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.merge.html" ] }, { "source": [ "What other data can we include? Are there other file types that we can work with?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 11, "metadata": {}, "outputs": [], "source": [ "# Read in misc data from excel\n", "misc_filepath = './data/misc_data.xlsx'" ] }, { "cell_type": "code", "execution_count": 12, "metadata": {}, "outputs": [], "source": [ "# .read_excel() gets the first sheet in the excel file. Instead, get the \"Income\" sheet" ] }, { "cell_type": "code", "execution_count": 14, "metadata": {}, "outputs": [], "source": [ "# Get \"Population\" sheet" ] }, { "cell_type": "code", "execution_count": 15, "metadata": {}, "outputs": [], "source": [ "# Get \"Population\" sheet with proper rows" ] }, { "cell_type": "code", "execution_count": 16, "metadata": {}, "outputs": [], "source": [ "# Do we need all of these columns?" ] }, { "cell_type": "code", "execution_count": 17, "metadata": {}, "outputs": [], "source": [ "# Select total population column" ] }, { "cell_type": "code", "execution_count": 18, "metadata": {}, "outputs": [], "source": [ "# Merge totial population df with eur_data df" ] }, { "cell_type": "code", "execution_count": 19, "metadata": {}, "outputs": [], "source": [ "# Select population with country names, so pandas can perform merge" ] }, { "cell_type": "code", "execution_count": 20, "metadata": {}, "outputs": [], "source": [ "# Perform merge and sort values by country name" ] }, { "source": [ "It looks like there's an issue with our indexes. What is an index, anyway?" ], "cell_type": "markdown", "metadata": {} }, { "source": [ "## 1.4 Indexes\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.Index.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 21, "metadata": {}, "outputs": [], "source": [ "# Our data is indexed by integer values" ] }, { "cell_type": "code", "execution_count": 22, "metadata": {}, "outputs": [], "source": [ "# Indexes are immutable. And for good reason..." ] }, { "cell_type": "code", "execution_count": 23, "metadata": {}, "outputs": [], "source": [ "# Dataframes can also be indexed by labels, rather than numbers.\n", "# Use the .set_index() method to set the index for our dataframe" ] }, { "source": [ "## 1.5 Exporting Data\n", "Pandas has robust I/O functionality." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 128, "metadata": {}, "outputs": [], "source": [ "# Helper function for generating a filepath\n", "def get_filepath(filename, extension):\n", " return './data/out/' + filename + '.' + extension" ] }, { "cell_type": "code", "execution_count": 129, "metadata": {}, "outputs": [], "source": [ "# Write df to csv using helper function" ] }, { "source": [ "### Exercise - Write this dataframe to Excel, JSON, and HTML" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 131, "metadata": {}, "outputs": [], "source": [ "# Excel (.xlsx)" ] }, { "cell_type": "code", "execution_count": 132, "metadata": {}, "outputs": [], "source": [ "# JSON (.json)" ] }, { "cell_type": "code", "execution_count": 133, "metadata": {}, "outputs": [], "source": [ "# HTML (.html)" ] }, { "source": [ "## 2.1 Working with DataFrames\n", "Let's work with an existing dataframe that includes a lot of the same data from the previous sections." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 24, "metadata": {}, "outputs": [], "source": [ "# Read in the complete version of the europe data, using the first column as the index" ] }, { "cell_type": "code", "execution_count": 25, "metadata": {}, "outputs": [], "source": [ "# Inspect the data" ] }, { "cell_type": "code", "execution_count": 26, "metadata": {}, "outputs": [], "source": [ "# Get the average of all the columns" ] }, { "source": [ "## 2.2 Boolean Indexing\n", "First of all, what is the boolean data type? It is data type that represents one of two possible values. For example, True False, On Off, etc." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 27, "metadata": {}, "outputs": [], "source": [ "# Select all rows with the country name equal to Austria" ] }, { "cell_type": "code", "execution_count": 28, "metadata": {}, "outputs": [], "source": [ "# Now do the same with bracket notation for the country of Greece" ] }, { "source": [ "Queries can also be generated using partial matches." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 29, "metadata": {}, "outputs": [], "source": [ "# Get all countries with the letter l in their name" ] }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [], "source": [ "# Notice that there is one country missing: Luxembourg. Why is that?" ] }, { "source": [ "## 2.3 Multiple queries\n", "So far, we've looked at queries with a single condition. Let's look at multiple query conditions." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 30, "metadata": {}, "outputs": [], "source": [ "# Create bool series for countries with unemployment less than 7" ] }, { "cell_type": "code", "execution_count": 31, "metadata": {}, "outputs": [], "source": [ "# Combine with this l_names series" ] }, { "cell_type": "code", "execution_count": 32, "metadata": {}, "outputs": [], "source": [ "# In set theory, this is intersection. i.e. 'and'" ] }, { "cell_type": "code", "execution_count": 33, "metadata": {}, "outputs": [], "source": [ "# In set theory, this is union. i.e. 'or'" ] }, { "cell_type": "code", "execution_count": 34, "metadata": {}, "outputs": [], "source": [ "# In set theory, this is complement. i.e. 'not'" ] }, { "source": [ "### Exercise - What countries have an unemployment rate greater than 9% and a GDP less than 280000?" ], "cell_type": "markdown", "metadata": {} }, { "source": [ "### Exercise - What countries have a median income between 10,000 and 20,000?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 35, "metadata": {}, "outputs": [], "source": [ "# Create a bool series from the df that combines these two conditions" ] }, { "cell_type": "code", "execution_count": 36, "metadata": {}, "outputs": [], "source": [ "# Alternative, using .between()" ] }, { "source": [ "## 3.1 Selection\n", "Using .loc(), .iloc()" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 159, "metadata": {}, "outputs": [], "source": [ "# Data from the eur_data_final df, represented as a python dictionary\n", "countries_dict = {\n", " 15: {'country': 'Italy', \n", " 'unemp_rate': 11.7, \n", " 'gdp': 1689824.0, \n", " 'median_income': 16237, \n", " 'total_pop': 59433744\n", " }\n", "}" ] }, { "cell_type": "code", "execution_count": 37, "metadata": {}, "outputs": [], "source": [ "# With vanilla python, how do we get the word 'Italy' from a dictionary?" ] }, { "cell_type": "code", "execution_count": 38, "metadata": {}, "outputs": [], "source": [ "# How do we do this with a dataframe?" ] }, { "cell_type": "code", "execution_count": 39, "metadata": {}, "outputs": [], "source": [ "# We can also get multiple columns" ] }, { "cell_type": "code", "execution_count": 40, "metadata": {}, "outputs": [], "source": [ "# Or an entire row/entry" ] }, { "cell_type": "code", "execution_count": 41, "metadata": {}, "outputs": [], "source": [ "# Or multiple rows and columns" ] }, { "cell_type": "code", "execution_count": 42, "metadata": {}, "outputs": [], "source": [ "# We can also use python's index slicing syntax" ] }, { "cell_type": "code", "execution_count": 43, "metadata": {}, "outputs": [], "source": [ "# Select by column value (Pandas is smart!)" ] }, { "source": [ "### Exercise - What countries have a higher unemployment rate than Slovenia and have a lowercase 't' in their name?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 44, "metadata": {}, "outputs": [], "source": [ "# Select slovenia unemployment values\n", "\n", "# Generate comparison query\n", "\n", "# Generate 'contains' query\n", "\n", "# Make selection using queries" ] }, { "source": [ "### Exercise - Generate a correlation matrix for the various columns and plot them using matplotlib" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 45, "metadata": {}, "outputs": [], "source": [ "# Select columns\n", "\n", "# Make subplot and figure\n", "\n", "# Generate correlation matrix\n", "\n", "# Generate matplotlib plot\n", "\n", "# Add colorbar to figure\n", "\n", "# Set tick labels" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] } ] } ================================================ FILE: intro_data_science/part_3/ds_part_3_complete.ipynb ================================================ { "metadata": { "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" }, "orig_nbformat": 2, "kernelspec": { "name": "python3", "display_name": "Python 3", "language": "python" } }, "nbformat": 4, "nbformat_minor": 2, "cells": [ { "source": [ "# A Brief Introduction to Pandas\n", "### Part 1\n", "\n", "In this notebook, we will cover the basics of Pandas, a dynamic, powerful Python library that is ubiquitous in the field of Data Science. It has robust I/O functionality and makes working with data a lot simpler than doing so in Excel. Let's get stared." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [], "source": [ "import pandas as pd\n", "import matplotlib.pyplot as plt" ] }, { "source": [ "## 1.1 DataFrames\n", "Let's read in some existing data using Pandas convenient read_csv method." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 2, "metadata": {}, "outputs": [], "source": [ "unemployment_df = pd.read_csv('./data/unemployment_2016.csv')" ] }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "pandas.core.frame.DataFrame" ] }, "metadata": {}, "execution_count": 3 } ], "source": [ "# Check the type\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.html\n", "type(unemployment_df)" ] }, { "cell_type": "code", "execution_count": 4, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "\nRangeIndex: 32 entries, 0 to 31\nData columns (total 2 columns):\n # Column Non-Null Count Dtype \n--- ------ -------------- ----- \n 0 country 32 non-null object \n 1 unemp_rate 32 non-null float64\ndtypes: float64(1), object(1)\nmemory usage: 640.0+ bytes\n" ] } ], "source": [ "unemployment_df.info()" ] }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " unemp_rate\n", "count 32.000000\n", "mean 8.337500\n", "std 4.393378\n", "min 3.000000\n", "25% 5.700000\n", "50% 7.300000\n", "75% 9.800000\n", "max 23.600000" ], "text/html": "
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unemp_rate
count32.000000
mean8.337500
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min3.000000
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\n
" }, "metadata": {}, "execution_count": 5 } ], "source": [ "unemployment_df.describe()" ] }, { "cell_type": "code", "execution_count": 6, "metadata": {}, "outputs": [], "source": [ "# Select a single column\n", "# Using bracket notation\n", "countries = unemployment_df['country']\n", "# Using dot notation\n", "countries = unemployment_df.country" ] }, { "cell_type": "code", "execution_count": 7, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "KeyError", "evalue": "0", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mKeyError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/indexes/base.py\u001b[0m in \u001b[0;36mget_loc\u001b[0;34m(self, key, method, tolerance)\u001b[0m\n\u001b[1;32m 3079\u001b[0m \u001b[0;32mtry\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m-> 3080\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m_engine\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mget_loc\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mcasted_key\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 3081\u001b[0m \u001b[0;32mexcept\u001b[0m \u001b[0mKeyError\u001b[0m \u001b[0;32mas\u001b[0m \u001b[0merr\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32mpandas/_libs/index.pyx\u001b[0m in \u001b[0;36mpandas._libs.index.IndexEngine.get_loc\u001b[0;34m()\u001b[0m\n", "\u001b[0;32mpandas/_libs/index.pyx\u001b[0m in \u001b[0;36mpandas._libs.index.IndexEngine.get_loc\u001b[0;34m()\u001b[0m\n", "\u001b[0;32mpandas/_libs/hashtable_class_helper.pxi\u001b[0m in \u001b[0;36mpandas._libs.hashtable.PyObjectHashTable.get_item\u001b[0;34m()\u001b[0m\n", "\u001b[0;32mpandas/_libs/hashtable_class_helper.pxi\u001b[0m in \u001b[0;36mpandas._libs.hashtable.PyObjectHashTable.get_item\u001b[0;34m()\u001b[0m\n", "\u001b[0;31mKeyError\u001b[0m: 0", "\nThe above exception was the direct cause of the following exception:\n", "\u001b[0;31mKeyError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Access by numeric index\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0munemployment_df\u001b[0m\u001b[0;34m[\u001b[0m\u001b[0;36m0\u001b[0m\u001b[0;34m]\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/frame.py\u001b[0m in \u001b[0;36m__getitem__\u001b[0;34m(self, key)\u001b[0m\n\u001b[1;32m 3022\u001b[0m \u001b[0;32mif\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mcolumns\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mnlevels\u001b[0m \u001b[0;34m>\u001b[0m \u001b[0;36m1\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 3023\u001b[0m \u001b[0;32mreturn\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m_getitem_multilevel\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mkey\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m-> 3024\u001b[0;31m \u001b[0mindexer\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mcolumns\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mget_loc\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mkey\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 3025\u001b[0m \u001b[0;32mif\u001b[0m \u001b[0mis_integer\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mindexer\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 3026\u001b[0m \u001b[0mindexer\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0;34m[\u001b[0m\u001b[0mindexer\u001b[0m\u001b[0;34m]\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/indexes/base.py\u001b[0m in \u001b[0;36mget_loc\u001b[0;34m(self, key, method, tolerance)\u001b[0m\n\u001b[1;32m 3080\u001b[0m \u001b[0;32mreturn\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m_engine\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mget_loc\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mcasted_key\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 3081\u001b[0m \u001b[0;32mexcept\u001b[0m \u001b[0mKeyError\u001b[0m \u001b[0;32mas\u001b[0m \u001b[0merr\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m-> 3082\u001b[0;31m \u001b[0;32mraise\u001b[0m \u001b[0mKeyError\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mkey\u001b[0m\u001b[0;34m)\u001b[0m \u001b[0;32mfrom\u001b[0m \u001b[0merr\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 3083\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 3084\u001b[0m \u001b[0;32mif\u001b[0m \u001b[0mtolerance\u001b[0m \u001b[0;32mis\u001b[0m \u001b[0;32mnot\u001b[0m \u001b[0;32mNone\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mKeyError\u001b[0m: 0" ] } ], "source": [ "# Access by numeric index\n", "unemployment_df[0]" ] }, { "cell_type": "code", "execution_count": 8, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "pandas.core.series.Series" ] }, "metadata": {}, "execution_count": 8 } ], "source": [ "# Check the type\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.Series.html\n", "type(countries)" ] }, { "cell_type": "code", "execution_count": 9, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "AttributeError", "evalue": "'Series' object has no attribute 'info'", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mAttributeError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Series objects do not have the same methods as dataframes\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0mcountries\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0minfo\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 3\u001b[0m \u001b[0mcountries\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mdescribe\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/generic.py\u001b[0m in \u001b[0;36m__getattr__\u001b[0;34m(self, name)\u001b[0m\n\u001b[1;32m 5458\u001b[0m \u001b[0;32mif\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m_info_axis\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m_can_hold_identifiers_and_holds_name\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mname\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 5459\u001b[0m \u001b[0;32mreturn\u001b[0m \u001b[0mself\u001b[0m\u001b[0;34m[\u001b[0m\u001b[0mname\u001b[0m\u001b[0;34m]\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m-> 5460\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0mobject\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m__getattribute__\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mself\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mname\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 5461\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 5462\u001b[0m \u001b[0;32mdef\u001b[0m \u001b[0m__setattr__\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mself\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mname\u001b[0m\u001b[0;34m:\u001b[0m \u001b[0mstr\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mvalue\u001b[0m\u001b[0;34m)\u001b[0m \u001b[0;34m->\u001b[0m \u001b[0;32mNone\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mAttributeError\u001b[0m: 'Series' object has no attribute 'info'" ] } ], "source": [ "# Series objects do not have the same methods as dataframes\n", "countries.info()\n", "countries.describe()" ] }, { "source": [ "## 1.2 Vectorized Operations\n", "In Pandas, operations work on each value in most data structures, such as dataframes and series." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 10, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "0 Austria is in Europe.\n", "1 Belgium is in Europe.\n", "2 Bulgaria is in Europe.\n", "3 Switzerland is in Europe.\n", "4 Cyprus is in Europe.\n", "5 Czechia is in Europe.\n", "6 Germany is in Europe.\n", "7 Denmark is in Europe.\n", "8 Estonia is in Europe.\n", "9 Greece is in Europe.\n", "10 Spain is in Europe.\n", "11 Finland is in Europe.\n", "12 France is in Europe.\n", "13 Croatia is in Europe.\n", "14 Hungary is in Europe.\n", "15 Ireland is in Europe.\n", "16 Iceland is in Europe.\n", "17 Italy is in Europe.\n", "18 Lithuania is in Europe.\n", "19 Luxembourg is in Europe.\n", "20 Latvia is in Europe.\n", "21 Malta is in Europe.\n", "22 Netherlands is in Europe.\n", "23 Norway is in Europe.\n", "24 Poland is in Europe.\n", "25 Portugal is in Europe.\n", "26 Romania is in Europe.\n", "27 Sweden is in Europe.\n", "28 Slovenia is in Europe.\n", "29 Slovakia is in Europe.\n", "30 Turkey is in Europe.\n", "31 United Kingdom is in Europe.\n", "Name: country, dtype: object" ] }, "metadata": {}, "execution_count": 10 } ], "source": [ "# Add a string to the countries series\n", "countries + \" is in Europe.\"" ] }, { "cell_type": "code", "execution_count": 11, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "TypeError", "evalue": "can only concatenate str (not \"int\") to str", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mTypeError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/ops/array_ops.py\u001b[0m in \u001b[0;36m_na_arithmetic_op\u001b[0;34m(left, right, op, is_cmp)\u001b[0m\n\u001b[1;32m 141\u001b[0m \u001b[0;32mtry\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m--> 142\u001b[0;31m \u001b[0mresult\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0mexpressions\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mevaluate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mop\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mleft\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mright\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 143\u001b[0m \u001b[0;32mexcept\u001b[0m \u001b[0mTypeError\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/computation/expressions.py\u001b[0m in \u001b[0;36mevaluate\u001b[0;34m(op, a, b, use_numexpr)\u001b[0m\n\u001b[1;32m 234\u001b[0m \u001b[0;31m# error: \"None\" not callable\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m--> 235\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0m_evaluate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mop\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mop_str\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0ma\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mb\u001b[0m\u001b[0;34m)\u001b[0m \u001b[0;31m# type: ignore[misc]\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 236\u001b[0m \u001b[0;32mreturn\u001b[0m \u001b[0m_evaluate_standard\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mop\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mop_str\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0ma\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mb\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/computation/expressions.py\u001b[0m in \u001b[0;36m_evaluate_standard\u001b[0;34m(op, op_str, a, b)\u001b[0m\n\u001b[1;32m 68\u001b[0m \u001b[0;32mwith\u001b[0m \u001b[0mnp\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0merrstate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mall\u001b[0m\u001b[0;34m=\u001b[0m\u001b[0;34m\"ignore\"\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m---> 69\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0mop\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0ma\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mb\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 70\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mTypeError\u001b[0m: can only concatenate str (not \"int\") to str", "\nDuring handling of the above exception, another exception occurred:\n", "\u001b[0;31mTypeError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Add a number to the countries series\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0mcountries\u001b[0m \u001b[0;34m+\u001b[0m \u001b[0;36m12\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/ops/common.py\u001b[0m in \u001b[0;36mnew_method\u001b[0;34m(self, other)\u001b[0m\n\u001b[1;32m 63\u001b[0m \u001b[0mother\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0mitem_from_zerodim\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mother\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 64\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m---> 65\u001b[0;31m \u001b[0;32mreturn\u001b[0m \u001b[0mmethod\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mself\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mother\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 66\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 67\u001b[0m \u001b[0;32mreturn\u001b[0m \u001b[0mnew_method\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/arraylike.py\u001b[0m in \u001b[0;36m__add__\u001b[0;34m(self, other)\u001b[0m\n\u001b[1;32m 87\u001b[0m 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"\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/ops/array_ops.py\u001b[0m in \u001b[0;36marithmetic_op\u001b[0;34m(left, right, op)\u001b[0m\n\u001b[1;32m 187\u001b[0m \u001b[0;32melse\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 188\u001b[0m \u001b[0;32mwith\u001b[0m \u001b[0mnp\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0merrstate\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mall\u001b[0m\u001b[0;34m=\u001b[0m\u001b[0;34m\"ignore\"\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m--> 189\u001b[0;31m \u001b[0mres_values\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0m_na_arithmetic_op\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mlvalues\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mrvalues\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mop\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 190\u001b[0m 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"metadata": {}, "outputs": [], "source": [ "# Sort the dataframe by rate\n", "unemployment_sorted_asc = unemployment_df.sort_values('unemp_rate', ascending=True)" ] }, { "cell_type": "code", "execution_count": 14, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate\n", "16 Iceland 3.0\n", "5 Czechia 4.0\n", "6 Germany 4.1\n", "23 Norway 4.7\n", "21 Malta 4.7" ], "text/html": "
\n\n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
countryunemp_rate
16Iceland3.0
5Czechia4.0
6Germany4.1
23Norway4.7
21Malta4.7
\n
" }, "metadata": {}, "execution_count": 14 } ], "source": [ "# Use .head() to get the top five values\n", "unemployment_sorted_asc.head()" ] }, { "cell_type": "code", "execution_count": 15, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate\n", "17 Italy 11.7\n", "4 Cyprus 13.0\n", "13 Croatia 13.1\n", "10 Spain 19.6\n", "9 Greece 23.6" ], "text/html": "
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countryunemp_rate
17Italy11.7
4Cyprus13.0
13Croatia13.1
10Spain19.6
9Greece23.6
\n
" }, "metadata": {}, "execution_count": 15 } ], "source": [ "# Use .tail() to get the five values\n", "unemployment_sorted_asc.tail()" ] }, { "source": [ "### Exercise - What is the average unemployment of the seven countries with the highest unemployment in europe?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 16, "metadata": {}, "outputs": [], "source": [ "# Sort the values of the unemployment_df - descending or ascending?\n", "unemployment_sorted_desc = unemployment_df.sort_values('unemp_rate', ascending=False)\n", "# Get the first seven rows in the returned series\n", "highest_unemployment = unemployment_sorted_desc.head(7)\n", "# Get the average of those rows\n", "highest_unemployment_avg = highest_unemployment['unemp_rate'].mean()" ] }, { "source": [ "### Extra - explore these methods:\n", ".min(), .max(), .sum(), .unique(), .nunique(), .count(), .duplicated()" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 17, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate\n", "0 Austria 6.0\n", "7 Denmark 6.2\n", "21 Malta 4.7\n", "22 Netherlands 6.0\n", "23 Norway 4.7\n", "24 Poland 6.2" ], "text/html": "
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countryunemp_rate
0Austria6.0
7Denmark6.2
21Malta4.7
22Netherlands6.0
23Norway4.7
24Poland6.2
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" }, "metadata": {}, "execution_count": 17 } ], "source": [ "unemployment_rates.min()\n", "unemployment_rates.max()\n", "unemployment_rates.sum()\n", "unemployment_rates.unique()\n", "unemployment_rates.nunique()\n", "unemployment_rates.count()\n", "dups = unemployment_rates.duplicated()\n", "dups_no_keep = unemployment_rates.duplicated(keep=False)\n", "unemployment_df[dups_no_keep]" ] }, { "source": [ "## 1.3 Merging DataFrames\n", "Data can come from a variety of different sources and it can be helpful to put them all in one place." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 18, "metadata": {}, "outputs": [], "source": [ "gdp_filepath = './data/gdp_2016.csv'\n", "# Read in data using this filepath\n", "gdp_df = pd.read_csv(gdp_filepath)" ] }, { "cell_type": "code", "execution_count": 19, "metadata": {}, "outputs": [ { "output_type": "stream", "name": "stdout", "text": [ "\nRangeIndex: 32 entries, 0 to 31\nData columns (total 2 columns):\n # Column Non-Null Count Dtype \n--- ------ -------------- ----- \n 0 country 32 non-null object \n 1 gdp 32 non-null float64\ndtypes: float64(1), object(1)\nmemory usage: 640.0+ bytes\n" ] }, { "output_type": "execute_result", "data": { "text/plain": [ " country gdp\n", "0 Cyprus 18490.2\n", "1 Latvia 25037.7\n", "2 Lithuania 38849.4\n", "3 Luxembourg 53303.0\n", "4 Hungary 113903.8" ], "text/html": "
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countrygdp
0Cyprus18490.2
1Latvia25037.7
2Lithuania38849.4
3Luxembourg53303.0
4Hungary113903.8
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" }, "metadata": {}, "execution_count": 19 } ], "source": [ "# Inspect the GDP data\n", "gdp_df.info()\n", "gdp_df.describe()\n", "gdp_df.head()" ] }, { "source": [ "Let's create a new dataframe by merging two existing dataframes. This is the one we'll use for the rest of section 2." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 20, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp\n", "0 Austria 6.0 356237.6\n", "1 Belgium 7.8 424660.3\n", "2 Bulgaria 7.6 48128.6\n", "3 Switzerland 5.0 605753.7\n", "4 Cyprus 13.0 18490.2" ], "text/html": "
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countryunemp_rategdp
0Austria6.0356237.6
1Belgium7.8424660.3
2Bulgaria7.648128.6
3Switzerland5.0605753.7
4Cyprus13.018490.2
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" }, "metadata": {}, "execution_count": 20 } ], "source": [ "# Merge unemployment df with gdp df\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.merge.html\n", "eur_data = pd.merge(unemployment_df, gdp_df)\n", "eur_data.head()" ] }, { "source": [ "What other data can we include? Are there other file types that we can work with?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 21, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country avg_hrs_worked\n", "0 Austria 36.5\n", "1 Belgium 37.0\n", "2 Bulgaria 40.8\n", "3 Switzerland 34.7\n", "4 Cyprus 39.2" ], "text/html": "
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countryavg_hrs_worked
0Austria36.5
1Belgium37.0
2Bulgaria40.8
3Switzerland34.7
4Cyprus39.2
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" }, "metadata": {}, "execution_count": 21 } ], "source": [ "# Read in misc data from excel\n", "misc_filepath = './data/misc_data.xlsx'\n", "misc_data = pd.read_excel(misc_filepath)\n", "misc_data.head()" ] }, { "cell_type": "code", "execution_count": 22, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country median_income\n", "0 Belgium 21335\n", "1 Bulgaria 6742\n", "2 Czechia 12478\n", "3 Denmark 21355\n", "4 Germany 21152" ], "text/html": "
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countrymedian_income
0Belgium21335
1Bulgaria6742
2Czechia12478
3Denmark21355
4Germany21152
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" }, "metadata": {}, "execution_count": 22 } ], "source": [ "# .read_excel() gets the first sheet in the excel file. Instead, get the \"Income\" sheet\n", "income_df = pd.read_excel('./data/misc_data.xlsx', \"Income\")\n", "income_df.head()" ] }, { "cell_type": "code", "execution_count": 23, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " Unnamed: 0 Unnamed: 1 \\\n", "0 This is an overview of the population data for... NaN \n", "1 NaN NaN \n", "2 country total_pop \n", "3 Belgium 11000638 \n", "4 Bulgaria 7364570 \n", "\n", " Unnamed: 2 \n", "0 NaN \n", "1 NaN \n", "2 prct_yng_adt_pop \n", "3 18.485146 \n", "4 18.432577 " ], "text/html": "
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Unnamed: 0Unnamed: 1Unnamed: 2
0This is an overview of the population data for...NaNNaN
1NaNNaNNaN
2countrytotal_popprct_yng_adt_pop
3Belgium1100063818.485146
4Bulgaria736457018.432577
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" }, "metadata": {}, "execution_count": 23 } ], "source": [ "pop_df = pd.read_excel('./data/misc_data.xlsx', 'Population')\n", "pop_df.head()" ] }, { "cell_type": "code", "execution_count": 24, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country total_pop prct_yng_adt_pop\n", "0 Belgium 11000638 18.485146\n", "1 Bulgaria 7364570 18.432577\n", "2 Estonia 1294455 19.688363\n", "3 Ireland 4574888 20.601335\n", "4 Greece 10816286 17.604416" ], "text/html": "
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countrytotal_popprct_yng_adt_pop
0Belgium1100063818.485146
1Bulgaria736457018.432577
2Estonia129445519.688363
3Ireland457488820.601335
4Greece1081628617.604416
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" }, "metadata": {}, "execution_count": 24 } ], "source": [ "pop_df = pd.read_excel('./data/misc_data.xlsx', 'Population', skiprows=3)\n", "pop_df.head()" ] }, { "cell_type": "code", "execution_count": 25, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "['country', 'total_pop', 'prct_yng_adt_pop']" ] }, "metadata": {}, "execution_count": 25 } ], "source": [ "# Do we need all of these columns?\n", "pop_df_col_names = pop_df.columns\n", "list(pop_df_col_names)" ] }, { "cell_type": "code", "execution_count": 26, "metadata": {}, "outputs": [], "source": [ "# Select total population column\n", "total_pop = pop_df['total_pop']" ] }, { "cell_type": "code", "execution_count": 27, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "MergeError", "evalue": "No common columns to perform merge on. Merge options: left_on=None, right_on=None, left_index=False, right_index=False", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mMergeError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[0;32m----> 1\u001b[0;31m \u001b[0meur_data\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0mpd\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mmerge\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0meur_data\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mtotal_pop\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/reshape/merge.py\u001b[0m in \u001b[0;36mmerge\u001b[0;34m(left, right, how, on, left_on, right_on, left_index, right_index, sort, suffixes, copy, indicator, validate)\u001b[0m\n\u001b[1;32m 72\u001b[0m \u001b[0mvalidate\u001b[0m\u001b[0;34m=\u001b[0m\u001b[0;32mNone\u001b[0m\u001b[0;34m,\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 73\u001b[0m ) -> \"DataFrame\":\n\u001b[0;32m---> 74\u001b[0;31m op = _MergeOperation(\n\u001b[0m\u001b[1;32m 75\u001b[0m \u001b[0mleft\u001b[0m\u001b[0;34m,\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 76\u001b[0m \u001b[0mright\u001b[0m\u001b[0;34m,\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/reshape/merge.py\u001b[0m in \u001b[0;36m__init__\u001b[0;34m(self, left, right, how, on, left_on, right_on, axis, left_index, right_index, sort, suffixes, copy, indicator, validate)\u001b[0m\n\u001b[1;32m 648\u001b[0m \u001b[0mwarnings\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mwarn\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mmsg\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mUserWarning\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 649\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m--> 650\u001b[0;31m \u001b[0mself\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0m_validate_specification\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 651\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 652\u001b[0m \u001b[0mcross_col\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0;32mNone\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/reshape/merge.py\u001b[0m in \u001b[0;36m_validate_specification\u001b[0;34m(self)\u001b[0m\n\u001b[1;32m 1281\u001b[0m \u001b[0mcommon_cols\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0mleft_cols\u001b[0m\u001b[0;34m.\u001b[0m\u001b[0mintersection\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mright_cols\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 1282\u001b[0m \u001b[0;32mif\u001b[0m \u001b[0mlen\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mcommon_cols\u001b[0m\u001b[0;34m)\u001b[0m \u001b[0;34m==\u001b[0m \u001b[0;36m0\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m-> 1283\u001b[0;31m raise MergeError(\n\u001b[0m\u001b[1;32m 1284\u001b[0m \u001b[0;34m\"No common columns to perform merge on. \"\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 1285\u001b[0m \u001b[0;34mf\"Merge options: left_on={self.left_on}, \"\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mMergeError\u001b[0m: No common columns to perform merge on. Merge options: left_on=None, right_on=None, left_index=False, right_index=False" ] } ], "source": [ "eur_data = pd.merge(eur_data, total_pop)" ] }, { "cell_type": "code", "execution_count": 28, "metadata": {}, "outputs": [], "source": [ "# Select population with country names, so pandas can perform merge\n", "total_pop_with_countries = pop_df[['country', 'total_pop']]" ] }, { "cell_type": "code", "execution_count": 29, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp total_pop\n", "0 Austria 6.0 356237.6 8401940\n", "1 Belgium 7.8 424660.3 11000638\n", "2 Bulgaria 7.6 48128.6 7364570\n", "13 Croatia 13.1 46639.5 4284889\n", "4 Cyprus 13.0 18490.2 840407" ], "text/html": "
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countryunemp_rategdptotal_pop
0Austria6.0356237.68401940
1Belgium7.8424660.311000638
2Bulgaria7.648128.67364570
13Croatia13.146639.54284889
4Cyprus13.018490.2840407
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" }, "metadata": {}, "execution_count": 29 } ], "source": [ "# Perform merge and sort values by country name\n", "eur_data = pd.merge(eur_data, total_pop_with_countries)\n", "eur_data_sorted = eur_data.sort_values('country')\n", "eur_data_sorted.head()" ] }, { "source": [ "It looks like there's an issue with our indexes. What is an index, anyway?" ], "cell_type": "markdown", "metadata": {} }, { "source": [ "## 1.4 Indexes\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.Index.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 30, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "pandas.core.indexes.numeric.Int64Index" ] }, "metadata": {}, "execution_count": 30 } ], "source": [ "# Our data is indexed by integer values\n", "eur_index = eur_data.index\n", "type(eur_index)" ] }, { "cell_type": "code", "execution_count": 31, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "TypeError", "evalue": "Index does not support mutable operations", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mTypeError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Indexes are immutable. And for good reason...\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0meur_index\u001b[0m\u001b[0;34m[\u001b[0m\u001b[0;36m0\u001b[0m\u001b[0;34m]\u001b[0m \u001b[0;34m=\u001b[0m \u001b[0;36m12\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/indexes/base.py\u001b[0m in \u001b[0;36m__setitem__\u001b[0;34m(self, key, value)\u001b[0m\n\u001b[1;32m 4275\u001b[0m \u001b[0;34m@\u001b[0m\u001b[0mfinal\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 4276\u001b[0m \u001b[0;32mdef\u001b[0m \u001b[0m__setitem__\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mself\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mkey\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mvalue\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m-> 4277\u001b[0;31m \u001b[0;32mraise\u001b[0m \u001b[0mTypeError\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0;34m\"Index does not support mutable operations\"\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m\u001b[1;32m 4278\u001b[0m \u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 4279\u001b[0m \u001b[0;32mdef\u001b[0m \u001b[0m__getitem__\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mself\u001b[0m\u001b[0;34m,\u001b[0m \u001b[0mkey\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mTypeError\u001b[0m: Index does not support mutable operations" ] } ], "source": [ "# Indexes are immutable. And for good reason...\n", "eur_index[0] = 12" ] }, { "cell_type": "code", "execution_count": 32, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " unemp_rate gdp total_pop\n", "country \n", "Austria 6.0 356237.6 8401940\n", "Belgium 7.8 424660.3 11000638\n", "Bulgaria 7.6 48128.6 7364570\n", "Croatia 13.1 46639.5 4284889\n", "Cyprus 13.0 18490.2 840407" ], "text/html": "
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unemp_rategdptotal_pop
country
Austria6.0356237.68401940
Belgium7.8424660.311000638
Bulgaria7.648128.67364570
Croatia13.146639.54284889
Cyprus13.018490.2840407
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" }, "metadata": {}, "execution_count": 32 } ], "source": [ "# Dataframes can also be indexed by labels, rather than numbers.\n", "# Use the .set_index() method to set the index for our dataframe\n", "eur_data_country_index = eur_data_sorted.set_index('country')\n", "eur_data_country_index.head()" ] }, { "source": [ "## 1.5 Exporting Data\n", "Pandas has robust I/O functionality." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 33, "metadata": {}, "outputs": [], "source": [ "# Helper function for generating a filepath\n", "def get_filepath(filename, extension):\n", " return './data/out/' + filename + '.' + extension" ] }, { "cell_type": "code", "execution_count": 34, "metadata": {}, "outputs": [], "source": [ "filepath = get_filepath('eur_data_sorted', 'csv')\n", "eur_data_sorted.to_csv(filepath)" ] }, { "source": [ "### Exercise - Write this dataframe to Excel, JSON, and HTML" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 35, "metadata": {}, "outputs": [], "source": [ "# Excel (.xlsx)\n", "eur_data_sorted.to_excel(get_filepath('eur_data_sorted', 'xlsx'))" ] }, { "cell_type": "code", "execution_count": 36, "metadata": {}, "outputs": [], "source": [ "# JSON (.json)\n", "eur_data_sorted.to_json(get_filepath('eur_data_sorted', 'json'))" ] }, { "cell_type": "code", "execution_count": 37, "metadata": {}, "outputs": [], "source": [ "# HTML (.html)\n", "eur_data_sorted.to_html(get_filepath('eur_data_sorted', 'html'))" ] }, { "source": [ "## 2.1 Working with DataFrames\n", "Let's work with an existing dataframe that includes a lot of the same data from the previous sections." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 38, "metadata": {}, "outputs": [], "source": [ "# Read in the complete version of the europe data, using the first column as the index\n", "eur_data_final = pd.read_csv('./data/complete/eur_data_final.csv', index_col=0)" ] }, { "cell_type": "code", "execution_count": 39, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " unemp_rate gdp median_income total_pop\n", "count 32.000000 3.200000e+01 32.000000 3.200000e+01\n", "mean 8.337500 5.218333e+05 15972.343750 1.637612e+07\n", "std 4.393378 7.781849e+05 6640.636617 2.198819e+07\n", "min 3.000000 1.034410e+04 4724.000000 3.155560e+05\n", "25% 5.700000 4.775632e+04 10190.500000 3.974524e+06\n", "50% 7.300000 2.012768e+05 16205.000000 7.954662e+06\n", "75% 9.800000 4.987990e+05 21161.250000 1.241443e+07\n", "max 23.600000 3.159750e+06 28663.000000 8.021970e+07" ], "text/html": "
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unemp_rategdpmedian_incometotal_pop
count32.0000003.200000e+0132.0000003.200000e+01
mean8.3375005.218333e+0515972.3437501.637612e+07
std4.3933787.781849e+056640.6366172.198819e+07
min3.0000001.034410e+044724.0000003.155560e+05
25%5.7000004.775632e+0410190.5000003.974524e+06
50%7.3000002.012768e+0516205.0000007.954662e+06
75%9.8000004.987990e+0521161.2500001.241443e+07
max23.6000003.159750e+0628663.0000008.021970e+07
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" }, "metadata": {}, "execution_count": 39 } ], "source": [ "# Inspect the data\n", "eur_data_final.head()\n", "eur_data_final.tail()\n", "eur_data_final.describe()" ] }, { "cell_type": "code", "execution_count": 40, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "unemp_rate 8.337500e+00\n", "gdp 5.218333e+05\n", "median_income 1.597234e+04\n", "total_pop 1.637612e+07\n", "dtype: float64" ] }, "metadata": {}, "execution_count": 40 } ], "source": [ "eur_data_final.mean()" ] }, { "source": [ "## 2.2 Boolean Indexing\n", "First of all, what is the boolean data type? It is data type that represents one of two possible values. For example, True False, On Off, etc." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 41, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "0 Austria 6.0 356237.6 23071 8401940" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
0Austria6.0356237.6230718401940
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" }, "metadata": {}, "execution_count": 41 } ], "source": [ "# Select all rows with the country name equal to Austria\n", "eur_data_final.country\n", "austria_bool = eur_data_final.country == 'Austria'\n", "eur_data_final[austria_bool]" ] }, { "cell_type": "code", "execution_count": 42, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "11 Greece 23.6 176487.9 9048 10816286" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
11Greece23.6176487.9904810816286
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" }, "metadata": {}, "execution_count": 42 } ], "source": [ "# Now do the same with bracket notation for the country of Greece\n", "greece_bool = eur_data_final['country'] == 'Greece'\n", "eur_data_final[greece_bool]" ] }, { "source": [ "Queries can also be generated using partial matches." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 43, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "1 Belgium 7.8 424660.3 21335 11000638\n", "2 Bulgaria 7.6 48128.6 6742 7364570\n", "8 Finland 8.8 216073.0 19997 5375276\n", "13 Iceland 3.0 18646.1 22193 315556\n", "14 Ireland 8.4 273238.2 18286 4574888\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "19 Malta 4.7 10344.1 17264 417432\n", "20 Netherlands 6.0 708337.0 21189 16655799\n", "22 Poland 6.2 426547.5 10865 38044565\n", "23 Portugal 11.2 186480.5 10805 10562178\n", "25 Slovakia 9.7 81226.1 10466 5397036\n", "26 Slovenia 8.0 40357.2 15250 2050189\n", "29 Switzerland 5.0 605753.7 27692 7954662" ], "text/html": "
\n\n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
countryunemp_rategdpmedian_incometotal_pop
1Belgium7.8424660.32133511000638
2Bulgaria7.648128.667427364570
8Finland8.8216073.0199975375276
13Iceland3.018646.122193315556
14Ireland8.4273238.2182864574888
15Italy11.71689824.01623759433744
19Malta4.710344.117264417432
20Netherlands6.0708337.02118916655799
22Poland6.2426547.51086538044565
23Portugal11.2186480.51080510562178
25Slovakia9.781226.1104665397036
26Slovenia8.040357.2152502050189
29Switzerland5.0605753.7276927954662
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" }, "metadata": {}, "execution_count": 43 } ], "source": [ "# Get all countries with the letter l in their name\n", "l_names = eur_data_final['country'].str.contains('l')\n", "eur_data_final[l_names]" ] }, { "cell_type": "code", "execution_count": 44, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "1 Belgium 7.8 424660.3 21335 11000638\n", "2 Bulgaria 7.6 48128.6 6742 7364570\n", "8 Finland 8.8 216073.0 19997 5375276\n", "13 Iceland 3.0 18646.1 22193 315556\n", "14 Ireland 8.4 273238.2 18286 4574888\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "16 Latvia 9.6 25037.7 9257 2070371\n", "17 Lithuania 7.9 38849.4 9364 3043429\n", "18 Luxembourg 6.3 53303.0 28663 512353\n", "19 Malta 4.7 10344.1 17264 417432\n", "20 Netherlands 6.0 708337.0 21189 16655799\n", "22 Poland 6.2 426547.5 10865 38044565\n", "23 Portugal 11.2 186480.5 10805 10562178\n", "25 Slovakia 9.7 81226.1 10466 5397036\n", "26 Slovenia 8.0 40357.2 15250 2050189\n", "29 Switzerland 5.0 605753.7 27692 7954662" ], "text/html": "
\n\n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
countryunemp_rategdpmedian_incometotal_pop
1Belgium7.8424660.32133511000638
2Bulgaria7.648128.667427364570
8Finland8.8216073.0199975375276
13Iceland3.018646.122193315556
14Ireland8.4273238.2182864574888
15Italy11.71689824.01623759433744
16Latvia9.625037.792572070371
17Lithuania7.938849.493643043429
18Luxembourg6.353303.028663512353
19Malta4.710344.117264417432
20Netherlands6.0708337.02118916655799
22Poland6.2426547.51086538044565
23Portugal11.2186480.51080510562178
25Slovakia9.781226.1104665397036
26Slovenia8.040357.2152502050189
29Switzerland5.0605753.7276927954662
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" }, "metadata": {}, "execution_count": 44 } ], "source": [ "# Notice that there is one country missing: Luxembourg. Why is that?\n", "l_names_insensitive = eur_data_final.country.str.contains('l', case=False)\n", "eur_data_final[l_names_insensitive]" ] }, { "source": [ "## 2.3 Multiple queries\n", "So far, we've looked at queries with a single condition. Let's look at multiple query conditions." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 45, "metadata": {}, "outputs": [], "source": [ "# Create bool series for countries with unemployment less than 7\n", "low_unemployment = eur_data_final.unemp_rate < 7" ] }, { "cell_type": "code", "execution_count": 46, "metadata": {}, "outputs": [ { "output_type": "error", "ename": "ValueError", "evalue": "The truth value of a Series is ambiguous. Use a.empty, a.bool(), a.item(), a.any() or a.all().", "traceback": [ "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", "\u001b[0;31mValueError\u001b[0m Traceback (most recent call last)", "\u001b[0;32m\u001b[0m in \u001b[0;36m\u001b[0;34m\u001b[0m\n\u001b[1;32m 1\u001b[0m \u001b[0;31m# Combine with this l_names series\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m----> 2\u001b[0;31m \u001b[0meur_data_final\u001b[0m\u001b[0;34m[\u001b[0m\u001b[0ml_names_insensitive\u001b[0m \u001b[0;32mand\u001b[0m \u001b[0mlow_unemployment\u001b[0m\u001b[0;34m]\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0m", "\u001b[0;32m~/Library/Python/3.8/lib/python/site-packages/pandas/core/generic.py\u001b[0m in \u001b[0;36m__nonzero__\u001b[0;34m(self)\u001b[0m\n\u001b[1;32m 1437\u001b[0m \u001b[0;34m@\u001b[0m\u001b[0mfinal\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 1438\u001b[0m \u001b[0;32mdef\u001b[0m \u001b[0m__nonzero__\u001b[0m\u001b[0;34m(\u001b[0m\u001b[0mself\u001b[0m\u001b[0;34m)\u001b[0m\u001b[0;34m:\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[0;32m-> 1439\u001b[0;31m raise ValueError(\n\u001b[0m\u001b[1;32m 1440\u001b[0m \u001b[0;34mf\"The truth value of a {type(self).__name__} is ambiguous. \"\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n\u001b[1;32m 1441\u001b[0m \u001b[0;34m\"Use a.empty, a.bool(), a.item(), a.any() or a.all().\"\u001b[0m\u001b[0;34m\u001b[0m\u001b[0;34m\u001b[0m\u001b[0m\n", "\u001b[0;31mValueError\u001b[0m: The truth value of a Series is ambiguous. Use a.empty, a.bool(), a.item(), a.any() or a.all()." ] } ], "source": [ "# Combine with this l_names series\n", "eur_data_final[l_names_insensitive and low_unemployment]" ] }, { "cell_type": "code", "execution_count": 47, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "13 Iceland 3.0 18646.1 22193 315556\n", "18 Luxembourg 6.3 53303.0 28663 512353\n", "19 Malta 4.7 10344.1 17264 417432\n", "20 Netherlands 6.0 708337.0 21189 16655799\n", "22 Poland 6.2 426547.5 10865 38044565\n", "29 Switzerland 5.0 605753.7 27692 7954662" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
13Iceland3.018646.122193315556
18Luxembourg6.353303.028663512353
19Malta4.710344.117264417432
20Netherlands6.0708337.02118916655799
22Poland6.2426547.51086538044565
29Switzerland5.0605753.7276927954662
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" }, "metadata": {}, "execution_count": 47 } ], "source": [ "# In set theory, this is intersection. i.e. 'and'\n", "eur_data_final[l_names_insensitive & low_unemployment]" ] }, { "cell_type": "code", "execution_count": 48, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "0 Austria 6.0 356237.6 23071 8401940\n", "1 Belgium 7.8 424660.3 21335 11000638\n", "2 Bulgaria 7.6 48128.6 6742 7364570\n", "5 Czechia 4.0 176370.1 12478 10436560\n", "6 Denmark 6.2 282089.9 21355 5560628\n", "7 Estonia 6.8 21682.6 11867 1294455\n", "8 Finland 8.8 216073.0 19997 5375276\n", "10 Germany 4.1 3159750.0 21152 80219695\n", "12 Hungary 5.1 113903.8 8267 9937628\n", "13 Iceland 3.0 18646.1 22193 315556\n", "14 Ireland 8.4 273238.2 18286 4574888\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "18 Luxembourg 6.3 53303.0 28663 512353\n", "19 Malta 4.7 10344.1 17264 417432\n", "20 Netherlands 6.0 708337.0 21189 16655799\n", "21 Norway 4.7 335747.5 27670 4979954\n", "22 Poland 6.2 426547.5 10865 38044565\n", "23 Portugal 11.2 186480.5 10805 10562178\n", "24 Romania 5.9 170393.6 4724 20121641\n", "25 Slovakia 9.7 81226.1 10466 5397036\n", "26 Slovenia 8.0 40357.2 15250 2050189\n", "29 Switzerland 5.0 605753.7 27692 7954662\n", "31 United Kingdom 4.8 2403382.6 17296 63182180" ], "text/html": "
\n\n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
countryunemp_rategdpmedian_incometotal_pop
0Austria6.0356237.6230718401940
1Belgium7.8424660.32133511000638
2Bulgaria7.648128.667427364570
5Czechia4.0176370.11247810436560
6Denmark6.2282089.9213555560628
7Estonia6.821682.6118671294455
8Finland8.8216073.0199975375276
10Germany4.13159750.02115280219695
12Hungary5.1113903.882679937628
13Iceland3.018646.122193315556
14Ireland8.4273238.2182864574888
15Italy11.71689824.01623759433744
18Luxembourg6.353303.028663512353
19Malta4.710344.117264417432
20Netherlands6.0708337.02118916655799
21Norway4.7335747.5276704979954
22Poland6.2426547.51086538044565
23Portugal11.2186480.51080510562178
24Romania5.9170393.6472420121641
25Slovakia9.781226.1104665397036
26Slovenia8.040357.2152502050189
29Switzerland5.0605753.7276927954662
31United Kingdom4.82403382.61729663182180
\n
" }, "metadata": {}, "execution_count": 48 } ], "source": [ "# In set theory, this is union. i.e. 'or'\n", "eur_data_final[l_names | low_unemployment]" ] }, { "cell_type": "code", "execution_count": 49, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "1 Belgium 7.8 424660.3 21335 11000638\n", "2 Bulgaria 7.6 48128.6 6742 7364570\n", "3 Croatia 13.1 46639.5 8985 4284889\n", "4 Cyprus 13.0 18490.2 16173 840407\n", "8 Finland 8.8 216073.0 19997 5375276\n", "9 France 10.1 2228568.0 20621 64933400\n", "11 Greece 23.6 176487.9 9048 10816286\n", "14 Ireland 8.4 273238.2 18286 4574888\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "16 Latvia 9.6 25037.7 9257 2070371\n", "17 Lithuania 7.9 38849.4 9364 3043429\n", "23 Portugal 11.2 186480.5 10805 10562178\n", "25 Slovakia 9.7 81226.1 10466 5397036\n", "26 Slovenia 8.0 40357.2 15250 2050189\n", "27 Spain 19.6 1118743.0 15347 46815910\n", "28 Sweden 7.0 463147.5 20955 9482855\n", "30 Turkey 10.9 780224.9 6501 7954662" ], "text/html": "
\n\n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
countryunemp_rategdpmedian_incometotal_pop
1Belgium7.8424660.32133511000638
2Bulgaria7.648128.667427364570
3Croatia13.146639.589854284889
4Cyprus13.018490.216173840407
8Finland8.8216073.0199975375276
9France10.12228568.02062164933400
11Greece23.6176487.9904810816286
14Ireland8.4273238.2182864574888
15Italy11.71689824.01623759433744
16Latvia9.625037.792572070371
17Lithuania7.938849.493643043429
23Portugal11.2186480.51080510562178
25Slovakia9.781226.1104665397036
26Slovenia8.040357.2152502050189
27Spain19.61118743.01534746815910
28Sweden7.0463147.5209559482855
30Turkey10.9780224.965017954662
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" }, "metadata": {}, "execution_count": 49 } ], "source": [ "# In set theory, this is complement. i.e. 'not'\n", "eur_data_final[~low_unemployment]" ] }, { "source": [ "### Exercise - What countries have an unemployment rate greater than 9% and a GDP less than 280000?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 62, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "9 France 10.1 2228568.0 20621 64933400\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "27 Spain 19.6 1118743.0 15347 46815910\n", "30 Turkey 10.9 780224.9 6501 7954662" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
9France10.12228568.02062164933400
15Italy11.71689824.01623759433744
27Spain19.61118743.01534746815910
30Turkey10.9780224.965017954662
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" }, "metadata": {}, "execution_count": 62 } ], "source": [ "unemp_gt_9 = eur_data_final.unemp_rate > 9\n", "gdp_lt_280 = eur_data_final.gdp > 280_000\n", "final = eur_data_final[unemp_gt_9 & gdp_lt_280]\n", "final" ] }, { "source": [ "### Exercise - What countries have a median income between 10,000 and 20,000?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 63, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "4 Cyprus 13.0 18490.2 16173 840407\n", "5 Czechia 4.0 176370.1 12478 10436560\n", "7 Estonia 6.8 21682.6 11867 1294455\n", "8 Finland 8.8 216073.0 19997 5375276\n", "14 Ireland 8.4 273238.2 18286 4574888\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "19 Malta 4.7 10344.1 17264 417432\n", "22 Poland 6.2 426547.5 10865 38044565\n", "23 Portugal 11.2 186480.5 10805 10562178\n", "25 Slovakia 9.7 81226.1 10466 5397036\n", "26 Slovenia 8.0 40357.2 15250 2050189\n", "27 Spain 19.6 1118743.0 15347 46815910\n", "31 United Kingdom 4.8 2403382.6 17296 63182180" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
4Cyprus13.018490.216173840407
5Czechia4.0176370.11247810436560
7Estonia6.821682.6118671294455
8Finland8.8216073.0199975375276
14Ireland8.4273238.2182864574888
15Italy11.71689824.01623759433744
19Malta4.710344.117264417432
22Poland6.2426547.51086538044565
23Portugal11.2186480.51080510562178
25Slovakia9.781226.1104665397036
26Slovenia8.040357.2152502050189
27Spain19.61118743.01534746815910
31United Kingdom4.82403382.61729663182180
\n
" }, "metadata": {}, "execution_count": 63 } ], "source": [ "# Create a bool series from the df that combines these two conditions\n", "income_query = (eur_data_final['median_income'] > 10_000) & (eur_data_final['median_income'].mean() < 20_000)\n", "eur_data_final[income_query]" ] }, { "cell_type": "code", "execution_count": 51, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "4 Cyprus 13.0 18490.2 16173 840407\n", "5 Czechia 4.0 176370.1 12478 10436560\n", "7 Estonia 6.8 21682.6 11867 1294455\n", "8 Finland 8.8 216073.0 19997 5375276\n", "14 Ireland 8.4 273238.2 18286 4574888\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "19 Malta 4.7 10344.1 17264 417432\n", "22 Poland 6.2 426547.5 10865 38044565\n", "23 Portugal 11.2 186480.5 10805 10562178\n", "25 Slovakia 9.7 81226.1 10466 5397036\n", "26 Slovenia 8.0 40357.2 15250 2050189\n", "27 Spain 19.6 1118743.0 15347 46815910\n", "31 United Kingdom 4.8 2403382.6 17296 63182180" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
4Cyprus13.018490.216173840407
5Czechia4.0176370.11247810436560
7Estonia6.821682.6118671294455
8Finland8.8216073.0199975375276
14Ireland8.4273238.2182864574888
15Italy11.71689824.01623759433744
19Malta4.710344.117264417432
22Poland6.2426547.51086538044565
23Portugal11.2186480.51080510562178
25Slovakia9.781226.1104665397036
26Slovenia8.040357.2152502050189
27Spain19.61118743.01534746815910
31United Kingdom4.82403382.61729663182180
\n
" }, "metadata": {}, "execution_count": 51 } ], "source": [ "# Alternative, using .between()\n", "income_query_alt = eur_data_final.median_income.between(10_000, 20_000, inclusive=True)\n", "eur_data_final[income_query_alt]" ] } ] } ================================================ FILE: intro_data_science/part_4/binder/requirements.txt ================================================ pandas==1.2.0 matplotlib==3.3.3 numpy==1.19.5 scipy==1.6.0 ================================================ FILE: intro_data_science/part_4/data/complete/eur_data_final.csv ================================================ ,country,unemp_rate,gdp,median_income,total_pop 0,Austria,6.0,356237.6,23071,8401940 1,Belgium,7.8,424660.3,21335,11000638 2,Bulgaria,7.6,48128.6,6742,7364570 3,Croatia,13.1,46639.5,8985,4284889 4,Cyprus,13.0,18490.2,16173,840407 5,Czechia,4.0,176370.1,12478,10436560 6,Denmark,6.2,282089.9,21355,5560628 7,Estonia,6.8,21682.6,11867,1294455 8,Finland,8.8,216073.0,19997,5375276 9,France,10.1,2228568.0,20621,64933400 10,Germany,4.1,3159750.0,21152,80219695 11,Greece,23.6,176487.9,9048,10816286 12,Hungary,5.1,113903.8,8267,9937628 13,Iceland,3.0,18646.1,22193,315556 14,Ireland,8.4,273238.2,18286,4574888 15,Italy,11.7,1689824.0,16237,59433744 16,Latvia,9.6,25037.7,9257,2070371 17,Lithuania,7.9,38849.4,9364,3043429 18,Luxembourg,6.3,53303.0,28663,512353 19,Malta,4.7,10344.1,17264,417432 20,Netherlands,6.0,708337.0,21189,16655799 21,Norway,4.7,335747.5,27670,4979954 22,Poland,6.2,426547.5,10865,38044565 23,Portugal,11.2,186480.5,10805,10562178 24,Romania,5.9,170393.6,4724,20121641 25,Slovakia,9.7,81226.1,10466,5397036 26,Slovenia,8.0,40357.2,15250,2050189 27,Spain,19.6,1118743.0,15347,46815910 28,Sweden,7.0,463147.5,20955,9482855 29,Switzerland,5.0,605753.7,27692,7954662 30,Turkey,10.9,780224.9,6501,7954662 31,United Kingdom,4.8,2403382.6,17296,63182180 ================================================ FILE: intro_data_science/part_4/data/complete/un_world_data.csv ================================================ country,Region,Surface area (km2),Population in thousands (2017),"Population density (per km2, 2017)","Sex ratio (m per 100 f, 2017)",GDP: Gross domestic product (million current US$),"GDP growth rate (annual %, const. 2005 prices)",GDP per capita (current US$),Economy: Agriculture (% of GVA),Economy: Industry (% of GVA),Economy: Services and other activity (% of GVA),Employment: Agriculture (% of employed),Employment: Industry (% of employed),Employment: Services (% of employed),Unemployment (% of labour force),Labour force participation (female/male pop. %),Agricultural production index (2004-2006=100),Food production index (2004-2006=100),International trade: Exports (million US$),International trade: Imports (million US$),International trade: Balance (million US$),"Balance of payments, current account (million US$)",Population growth rate (average annual %),Urban population (% of total population),Urban population growth rate (average annual %),"Fertility rate, total (live births per woman)","Life expectancy at birth (females/males, years)","Population age distribution (0-14 / 60+ years, %)",International migrant stock (000/% of total pop.),Refugees and others of concern to UNHCR (in thousands),Infant mortality rate (per 1000 live births,Health: Total expenditure (% of GDP),Health: Physicians (per 1000 pop.),Education: Government expenditure (% of GDP),Education: Primary gross enrol. ratio (f/m per 100 pop.),Education: Secondary gross enrol. ratio (f/m per 100 pop.),Education: Tertiary gross enrol. ratio (f/m per 100 pop.),Seats held by women in national parliaments %,Mobile-cellular subscriptions (per 100 inhabitants),Mobile-cellular subscriptions (per 100 inhabitants),Individuals using the Internet (per 100 inhabitants),Threatened species (number),Forested area (% of land area),CO2 emission estimates (million tons/tons per capita),"Energy production, primary (Petajoules)",Energy supply per capita (Gigajoules),"Pop. using improved drinking water (urban/rural, %)","Pop. using improved sanitation facilities (urban/rural, %)",Net Official Development Assist. received (% of GNI) Afghanistan,SouthernAsia,652864,35530,54.4,106.3,20270,-2.4,623.2,23.3,23.3,53.3,61.6,10.0,28.5,8.6,19.3/83.6,125,125,1458,3568,-2110,-5121,3.2,26.7,4.0,5.3,63.5/61.0,43.2/4.1,382.4/1.2,1513.1,68.6,8.2,0.3,3.3,91.1/131.6,39.7/70.7,3.7/13.3,27.7,61.6,8.3,42,2.1,9.8/0.3,63,5,78.2/47.0,45.1/27.0,21.43,-99 Albania,SouthernEurope,28748,2930,106.9,101.9,11541,2.6,3984.2,22.4,26.0,51.7,41.4,18.3,40.3,15.8,40.2/61.0,134,134,1962,4669,-2707,-1222,-0.1,57.4,2.2,1.7,79.9/75.6,17.4/19.0,57.6/2.0,8.8,14.6,5.9,1.3,3.5,111.7/115.5,92.5/98.8,68.1/48.7,22.9,106.4,63.3,130,28.2,5.7/2.0,84,36,94.9/95.2,95.5/90.2,2.96,-99 Armenia,WesternAsia,29743,2930,102.9,88.8,10529,3.0,3489.1,19.0,28.3,52.8,35.0,15.7,49.3,16.6,55.3/74.2,135,135,1776,3230,-1455,-279,0.3,62.7,-0.1,1.6,77.0/70.6,20.0/16.9,191.2/6.3,19.3,13.2,4.5,2.8,2.8,98.5/98.5,89.0/88.1,46.9/41.6,9.9,115.2,58.2,114,11.7,5.5/1.8,48,46,100.0/100.0,96.2/78.2,3.17,-99 Azerbaijan,WesternAsia,86600,9828,118.9,99.3,53049,0.7,5438.7,6.7,49.9,43.4,36.7,14.2,49.1,5.2,62.0/68.8,131,136,9143,8532,611,-222,1.3,54.6,1.6,2.1,74.6/68.6,23.3/10.1,264.2/2.7,623.3,31.4,6.0,3.4,2.6,105.6/107.4,-99,27.5/23.6,16.8,111.3,77.0,97,13.5,37.5/3.9,2459,61,94.7/77.8,91.6/86.6,0.14,-99 Belize,CentralAmerica,22966,375,16.4,99.2,1721,1.2,4789.4,14.6,18.5,66.9,16.1,15.9,67.9,11.5,56.7/83.8,97,97,246,952,-706,-175,2.2,44.0,1.9,2.6,72.7/67.2,31.4/6.2,53.9/15.0,3.1,14.3,5.8,...,6.4,110.4/115.8,81.8/79.8,28.7/17.9,9.4,48.9,41.6,117,60.1,0.5/1.4,9,36,98.9/100.0,93.5/88.2,1.68,-99 Benin,WesternAfrica,114763,11176,99.1,99.5,8476,5.2,779.1,23.2,24.9,51.9,43.2,10.2,46.6,1.0,70.0/73.4,152,158,410,2630,-2220,-885,2.8,44.0,3.7,5.2,61.4/58.5,42.7/5.0,245.4/2.3,0.8,67.7,4.6,0.1,4.4,123.7/134.2,46.8/66.7,8.4/22.4,7.2,85.6,6.8,88,38.7,6.3/0.6,96,17,85.2/72.1,35.6/7.3,5.09,-99 Bhutan,SouthernAsia,38394,808,21.2,113.1,2074,5.2,2677.1,17.2,43.9,38.8,56.6,9.7,33.7,2.4,59.4/73.5,98,98,616,1688,-1072,-579,1.6,38.6,3.7,2.2,68.9/68.6,26.5/7.3,51.1/6.6,-99,30.5,3.6,0.3,7.4,96.9/96.6,87.1/81.4,9.2/12.6,8.5,87.1,39.8,71,72.0,1.0/1.3,75,82,100.0/100.0,77.9/33.1,5.26,-99 Bolivia,SouthAmerica,1098581,11052,10.2,100.2,32998,4.8,3076.8,12.6,31.0,56.5,29.5,21.1,49.4,3.8,64.1/82.6,142,143,6969,8374,-1405,-1854,1.6,68.5,2.3,3.0,70.2/65.3,31.6/9.5,143.0/1.3,0.8,42.9,6.3,0.5,7.3,95.7/98.5,85.7/87.1,.../...,53.1,92.2,45.1,231,50.8,20.4/1.9,962,32,96.7/75.6,60.8/27.5,2.38,-99 Brazil,SouthAmerica,8515767,209288,25.0,96.6,1772591,-3.8,8528.3,5.2,22.7,72.0,15.2,21.5,63.2,12.4,56.0/78.2,135,136,185235,137552,47683,-58882,0.9,85.7,1.2,1.8,78.4/71.0,21.7/12.6,713.6/0.3,41.1,15.8,8.3,1.9,6.0,113.8/116.8,102.2/97.2,59.3/42.4,10.7,126.6,59.1,990,59.2,529.8/2.6,10948,61,100.0/87.0,88.0/51.5,0.06,-99 Burkina Faso,WesternAfrica,272967,19193,70.2,99.5,11065,4.1,611.1,34.5,21.8,43.7,80.0,4.9,15.1,2.9,76.5/90.5,131,134,2019,3699,-1680,-998,3.0,29.9,5.9,5.6,59.3/58.0,45.2/3.9,704.7/3.9,32.7,64.8,5.0,~0.0,4.1,86.1/89.9,32.2/35.1,3.8/7.3,11.0,80.6,11.4,31,19.8,2.8/0.1,123,9,97.5/75.8,50.4/6.7,9.11,-99 Burundi,EasternAfrica,27830,10864,423.1,96.9,2735,-4.1,244.6,36.3,13.9,49.8,91.1,2.6,6.3,1.7,84.6/82.7,109,122,123,625,-502,-375,3.0,12.1,5.7,6.0,58.0/54.2,45.0/4.4,286.8/2.6,103.2,77.8,7.5,...,5.4,124.5/123.1,40.5/44.5,2.4/7.7,36.4,46.2,4.9,61,10.6,0.4/~0.0,56,6,91.1/73.8,43.8/48.6,11.91,-99 Cambodia,South-easternAsia,181035,16005,90.7,95.3,18050,7.0,1158.7,28.2,29.4,42.3,42.4,19.6,38.0,0.3,75.6/87.0,175,177,13204,15313,-2109,-1657,1.6,20.7,2.6,2.7,69.6/65.5,31.3/7.1,74.0/0.5,0.3,29.9,5.7,0.2,1.9,116.2/117.1,.../...,11.8/14.3,20.3,133.0,19.0,255,54.3,6.7/0.4,178,17,100.0/69.1,88.1/30.5,3.97,-99 Cameroon,MiddleAfrica,475650,24054,50.9,100.2,28416,5.8,1217.3,22.7,28.3,49.1,61.8,8.7,29.5,4.6,71.1/81.2,155,160,2130,4899,-2768,-1173,2.7,54.4,3.6,5.0,57.7/55.1,42.7/4.8,382.0/1.6,544.9,67.5,4.1,...,3.0,110.7/123.5,53.5/62.6,15.2/19.7,31.1,71.8,20.7,775,40.3,7.0/0.3,408,14,94.8/52.7,61.8/26.8,2.31,-99 Chile,SouthAmerica,756102,18055,24.3,98.2,240796,2.3,13416.2,3.9,32.8,63.3,9.6,22.9,67.5,6.8,50.7/74.7,111,111,59884,58804,1080,-4761,0.9,89.5,1.1,1.8,81.3/76.2,20.3/16.0,469.4/2.6,3.7,7.4,7.8,...,4.9,100.0/103.3,101.3/100.0,94.4/83.0,15.8,129.5,64.3,197,23.4,82.6/4.7,540,85,99.7/93.3,100.0/90.9,0.02,-99 China,EasternAsia,9600000,1409517,150.1,106.3,11158457,6.9,8109.1,9.2,41.1,49.7,27.0,23.9,49.1,4.6,63.0/77.7,132,133,2118981,1588696,530285,330602,0.5,55.6,3.0,1.6,77.2/74.2,17.7/16.2,978.0/0.1,301.7,11.6,5.5,1.5,-99,104.3/104.0,95.6/93.2,47.3/39.9,23.7,93.2,50.3,1080,22.0,10291.9/7.5,101394,87,97.5/93.0,86.6/63.7,0.00,-99 Colombia,SouthAmerica,1141748,49066,44.2,96.8,292080,3.1,6056.1,6.8,34.0,59.2,13.5,16.6,69.9,10.5,58.0/79.8,114,115,31045,44831,-13786,-18922,1.0,76.4,1.7,1.9,77.4/70.2,23.5/11.6,133.1/0.3,7127.0,17.9,7.2,...,4.5,111.6/115.4,101.5/94.8,59.9/51.5,18.7,115.7,55.9,835,52.8,84.1/1.8,5380,31,96.8/73.8,85.2/67.9,0.47,-99 Congo,MiddleAfrica,342000,5261,15.4,100.1,8493,1.2,1838.1,4.7,70.0,25.3,40.7,25.8,33.5,11.5,67.1/72.6,106,106,2540,9793,-7253,...,2.6,65.4,3.2,4.9,64.1/61.0,42.3/5.2,393.0/8.5,54.9,46.5,5.2,...,...,114.8/107.0,50.6/58.4,8.3/11.1,7.4,111.7,7.6,134,65.4,3.1/0.7,648,24,95.8/40.0,20.0/5.6,1.05,-99 Costa Rica,CentralAmerica,51100,4906,96.1,100.1,52958,3.7,11015.0,5.1,21.5,73.4,11.6,19.1,69.3,8.6,46.9/76.5,127,129,9908,15322,-5414,-2493,1.1,76.8,2.7,1.9,81.7/76.7,21.6/13.6,421.7/8.8,10.1,9.3,9.3,1.2,7.2,109.5/110.1,125.6/120.7,60.9/46.6,35.1,150.7,59.8,340,53.4,7.8/1.6,110,44,99.6/91.9,95.2/92.3,0.22,-99 Cote D'Ivoire,WesternAfrica,322463,24295,76.4,102.7,32076,9.5,1412.9,23.7,28.3,48.1,56.0,5.7,38.4,9.3,52.6/80.9,122,123,10661,8380,2281,-633,2.5,54.2,3.7,5.1,53.2/50.4,42.4/4.8,2175.4/9.6,1023.0,71.6,5.7,...,5.0,88.0/99.2,36.6/51.0,7.3/11.0,11.5,119.3,21.0,249,32.7,11.0/0.5,540,26,93.1/68.8,32.8/10.3,2.22,-99 Dominican Republic,Caribbean,48671,10767,222.8,99.2,67103,7.0,6373.6,6.6,28.0,65.4,13.1,16.9,70.0,14.4,52.3/78.6,136,138,3747,22725,-18978,-1335,1.2,79.0,2.6,2.5,76.4/70.2,29.3/10.2,415.6/3.9,1.3,25.1,4.4,1.5,...,98.7/108.1,81.7/74.1,65.0/35.4,26.8,82.6,51.9,184,40.4,21.5/2.1,28,28,85.4/81.9,86.2/75.7,0.43,-99 Ecuador,SouthAmerica,257217,16625,66.9,99.9,100177,0.2,6205.1,10.1,34.1,55.8,25.7,19.2,55.1,5.8,49.1/79.9,118,119,16798,16189,609,-2201,1.6,63.7,1.9,2.6,78.4/72.8,28.4/10.5,387.5/2.4,133.1,21.1,9.2,1.7,5.0,107.9/107.4,109.2/105.3,45.3/34.6,41.6,79.4,48.9,2358,50.8,43.9/2.8,1247,42,93.4/75.5,87.0/80.7,0.31,-99 Egypt,NorthernAfrica,1002000,97553,98.0,102.3,315917,4.2,3452.3,11.2,36.3,52.5,25.4,25.3,49.3,11.5,23.1/76.2,120,122,22507,58053,-35545,-16786,2.2,43.1,1.7,3.4,73.0/68.7,33.5/7.9,491.6/0.5,256.5,18.9,5.6,0.8,...,103.8/104.1,85.9/86.3,35.6/36.9,14.9,111.0,35.9,156,0.1,201.9/2.2,3509,36,100.0/99.0,96.8/93.1,0.78,-99 El Salvador,CentralAmerica,21041,6378,307.8,88.5,25850,2.5,4219.4,10.8,25.8,63.3,18.6,20.2,61.2,6.4,49.5/79.5,111,117,5335,9855,-4519,-920,0.5,66.7,1.4,2.2,77.1/67.9,27.4/11.6,42.0/0.7,4.7,17.0,6.8,...,3.5,106.8/111.4,79.7/79.2,30.5/27.7,32.1,145.3,26.9,86,13.0,6.3/1.0,86,28,97.5/86.5,82.4/60.0,0.36,-99 Georgia,WesternAsia,69700,3912,56.3,91.4,13965,2.8,3491.4,9.0,24.1,66.9,44.7,11.2,44.1,11.4,57.9/79.0,85,86,2114,7236,-5122,-1775,-1.4,53.6,-0.1,2.0,77.0/68.5,19.2/20.8,168.8/4.2,274.1,11.2,7.4,4.8,2.0,118.2/115.5,103.8/103.6,47.8/39.2,16.0,129.0,45.2,120,40.6,9.0/2.2,61,47,100.0/100.0,95.2/75.9,3.30,-99 Ghana,WesternAfrica,238537,28834,126.7,99.3,37156,3.9,1355.6,19.0,26.9,54.1,41.9,14.2,43.8,5.9,75.7/78.8,144,144,7221,11939,-4718,-2809,2.4,54.0,3.4,4.2,62.6/60.7,38.5/5.3,399.5/1.5,27.3,46.5,3.6,...,6.2,108.7/107.3,61.1/63.1,13.2/19.1,12.7,129.7,23.5,238,40.9,14.5/0.6,410,14,92.6/84.0,20.2/8.6,4.81,-99 Guam,Micronesia,549,164,304.1,102.6,-99,-99,-99,-99,-99,-99,0.2,14.0,85.7,10.7,55.2/68.7,88,88,-99,-99,-99,-99,0.3,94.5,1.4,2.4,81.5/76.4,24.7/14.0,76.1/44.8,-99,9.6,-99,-99,-99,-99,-99,-99,-99,...,73.1,99,46.3,-99,-99,-99,99.5/99.5,89.8/89.8,-99,-99 Guatemala,CentralAmerica,108889,16914,157.8,96.9,63794,4.1,3903.5,10.8,27.2,62.0,32.0,18.5,49.4,2.4,41.7/84.0,158,158,10572,16987,-6415,-96,2.1,51.6,3.4,3.2,75.6/69.2,35.1/6.9,76.4/0.5,1.8,26.9,6.2,...,3.0,99.8/103.8,63.3/67.9,23.5/20.2,12.7,111.5,27.1,290,33.4,18.3/1.1,327,32,98.4/86.8,77.5/49.3,0.66,-99 Haiti,Caribbean,27750,10981,398.4,97.8,8501,1.7,793.7,16.7,38.2,45.1,46.9,12.6,40.6,12.9,62.0/71.7,163,166,984,3316,-2332,-723,1.4,58.6,3.8,3.1,64.4/60.2,33.0/7.3,39.5/0.4,1.9,46.9,7.6,-99,-99,-99,-99,-99,2.6,69.9,12.2,205,3.5,2.9/0.3,136,16,64.9/47.6,33.6/19.2,11.73,-99 Honduras,CentralAmerica,112492,9265,82.8,99.4,20365,3.6,2521.9,13.0,25.1,61.9,29.4,21.6,49.0,5.6,47.6/84.5,122,119,3657,8448,-4791,-1291,1.8,54.7,3.1,2.6,75.4/70.4,31.6/7.0,28.1/0.3,176.2,27.8,8.7,...,5.9,110.1/111.3,77.0/64.8,25.4/18.8,25.8,95.5,20.4,301,42.1,9.5/1.2,105,28,97.4/83.8,86.7/77.7,2.86,-99 India,SouthernAsia,3287263,1339180,450.4,107.6,2116239,7.6,1614.2,17.0,29.7,53.2,44.3,24.5,31.2,3.4,27.0/79.1,143,142,260327,356705,-96378,-22457,1.2,32.7,2.4,2.4,69.1/66.2,27.8/9.4,5241.0/0.4,211.1,41.3,4.7,0.7,3.8,115.1/102.8,74.5/73.5,26.7/27.0,11.8,78.8,26.0,1052,23.7,2238.4/1.7,23103,27,97.1/92.6,62.6/28.5,0.16,-99 Indonesia,South-easternAsia,1910931,263991,145.7,101.4,861934,4.8,3346.5,14.0,41.3,44.7,31.4,22.4,46.2,5.8,51.0/83.7,139,140,144490,135653,8837,-17697,1.2,53.7,2.7,2.4,70.7/66.6,27.4/8.6,328.8/0.1,13.8,25.0,2.8,0.2,3.6,104.4/107.2,86.0/85.7,25.7/22.9,19.8,132.4,22.0,1281,50.6,464.2/1.8,19481,35,94.2/79.5,72.3/47.5,0.00,-99 Iraq,WesternAsia,435052,38275,88.1,102.5,164234,-2.4,4509.0,4.6,58.0,37.4,20.4,21.0,58.6,16.1,15.3/69.9,123,123,27341,45831,-18490,4121,3.2,69.5,3.0,4.6,71.4/67.0,40.4/5.0,353.9/1.0,4736.2,32.1,5.5,0.9,-99,.../...,.../...,.../...,25.3,93.8,17.2,72,1.9,168.4/4.8,6744,58,93.8/70.1,86.4/83.8,0.88,-99 Israel,WesternAsia,22072,8322,384.5,98.7,299413,2.5,37129.4,1.3,21.2,77.5,1.0,17.9,81.0,5.9,58.5/69.0,112,112,60571,65803,-5232,13642,1.6,92.1,1.4,3.0,83.7/80.0,27.9/16.1,2011.7/24.9,44.7,3.4,7.8,3.6,5.8,105.1/104.4,103.0/102.0,75.5/54.6,27.5,133.5,78.9,174,7.5,64.6/8.1,313,119,100.0/100.0,100.0/100.0,-99,-99 Jordan,WesternAsia,89318,9702,109.3,102.6,37517,2.4,4940.1,4.0,27.7,68.4,2.0,17.8,80.2,13.4,14.5/64.5,134,135,7509,19207,-11698,-3332,4.9,83.7,3.8,3.6,75.5/72.2,35.5/5.7,3112.0/41.0,721.4,17.1,7.5,2.6,-99,97.6/97.1,84.8/80.2,47.3/42.5,15.4,179.4,53.4,113,1.1,26.5/3.6,7,47,97.8/92.3,98.6/98.9,5.80,-99 Kenya,EasternAfrica,591958,49700,87.3,98.8,63399,5.6,1376.7,32.0,19.0,49.0,61.9,8.6,29.5,10.8,62.4/72.4,126,126,5688,14109,-8420,-6339,2.7,25.6,4.3,4.1,67.8/63.0,40.5/4.3,1084.4/2.4,582.4,39.4,5.7,0.2,5.3,108.7/109.3,.../...,.../...,19.4,80.7,45.6,480,7.7,14.3/0.3,750,20,81.6/56.8,31.2/29.7,3.93,-99 Kyrgyzstan,CentralAsia,199949,6045,31.5,98.4,6572,3.5,1106.4,15.4,25.9,58.7,29.2,21.0,49.8,7.7,49.7/77.5,108,110,1423,3844,-2421,-721,1.6,35.7,1.6,3.1,74.3/66.4,31.8/7.6,204.4/3.4,8.2,19.6,6.5,1.9,5.5,106.8/107.9,92.8/91.4,53.3/40.8,19.2,132.8,30.2,44,3.4,9.6/1.7,80,27,96.7/86.2,89.1/95.6,12.16,-99 Lao People's Democratic Republic,South-easternAsia,236800,6858,29.7,99.5,12585,7.6,1850.2,23.6,32.8,43.6,78.3,4.0,17.7,1.5,77.9/77.8,193,186,2066,4513,-2447,-2264,1.3,38.6,4.9,2.9,66.8/63.9,32.9/6.3,22.2/0.3,-99,47.3,1.9,0.2,3.3,109.1/113.5,59.3/64.1,16.5/17.3,27.5,53.1,18.2,209,80.5,2.0/0.3,72,12,85.6/69.4,94.5/56.0,4.03,-99 Lebanon,WesternAsia,10452,6082,594.6,100.6,50149,1.5,8571.4,3.2,19.6,77.2,8.2,22.4,69.4,7.0,23.8/70.6,95,95,3402,20409,-17007,-8146,6.0,87.8,3.2,1.7,80.9/77.3,23.1/12.0,1997.8/34.1,1054.2,9.2,6.4,2.4,2.6,88.3/96.6,61.0/61.5,45.7/39.5,3.1,87.1,74.0,87,13.4,24.1/4.3,7,55,99.0/99.0,80.7/80.7,2.04,-99 Lesotho,SouthernAfrica,30355,2233,73.6,94.4,2008,2.8,940.6,7.7,31.3,61.0,39.7,20.0,40.3,27.5,59.7/74.6,101,100,648,1727,-1079,-168,1.3,27.3,3.0,3.3,54.7/50.1,35.4/6.7,6.6/0.3,~0.0,59.8,10.6,...,...,104.0/107.0,62.0/45.7,11.7/8.0,25.0,105.5,16.1,18,1.6,2.5/1.2,31,28,94.6/77.0,37.3/27.6,4.11,-99 Liberia,WesternAfrica,111369,4732,49.1,101.8,2053,0.3,455.9,70.8,11.4,17.9,45.3,11.7,43.0,4.1,57.9/64.1,104,121,641,727,-86,-860,2.6,49.7,3.4,4.8,61.6/59.8,41.8/4.9,113.8/2.5,22.1,59.0,10.0,...,2.8,89.1/98.5,32.5/41.9,9.0/14.2,12.3,81.1,5.9,172,43.7,0.9/0.2,73,19,88.6/62.6,28.0/5.9,61.73,-99 Madagascar,EasternAfrica,587295,25571,44.0,99.5,9739,3.1,401.8,24.9,18.4,56.7,74.2,9.4,16.5,2.3,83.8/89.0,119,120,2256,2965,-709,-622,2.7,35.1,4.7,4.4,66.0/63.0,41.0/4.8,32.1/0.1,0.1,36.8,3.0,0.1,2.1,148.9/148.9,38.1/38.8,4.6/5.0,19.2,46.0,4.2,1324,21.5,3.1/0.1,129,7,81.6/35.3,18.0/8.7,7.05,-99 Malawi,EasternAfrica,118484,18622,197.5,98.2,6420,3.0,372.9,26.1,16.6,57.3,69.9,4.8,25.4,6.8,81.3/81.0,149,150,875,1649,-774,-710,2.9,16.3,3.8,4.9,63.1/58.2,44.0/4.3,215.2/1.2,25.7,66.5,11.4,...,5.6,147.0/144.0,41.0/45.8,0.6/1.0,16.7,35.3,9.3,176,33.6,1.3/0.1,89,6,95.7/89.1,47.3/39.8,16.53,-99 Mali,WesternAfrica,1240192,18542,15.2,100.2,13100,7.6,744.3,39.9,19.6,40.5,56.7,14.7,28.6,8.1,50.5/82.3,154,157,3030,2510,520,-676,2.9,39.9,5.1,6.4,56.9/55.6,47.7/4.0,363.1/2.1,90.8,78.5,7.0,...,3.7,72.1/79.3,36.8/45.6,4.1/9.6,8.8,139.6,10.3,42,3.9,1.4/0.1,55,4,96.5/64.1,37.5/16.1,9.45,-99 Mauritania,WesternAfrica,1030700,4420,4.3,101.6,5023,1.2,1235.0,20.9,41.7,37.4,40.3,9.5,50.2,12.0,29.3/65.5,125,125,1623,2174,-551,-1066,2.9,59.9,3.5,4.9,64.1/61.2,39.9/5.0,138.2/3.4,69.0,68.0,3.8,...,2.9,105.0/100.0,29.5/31.7,3.6/7.1,25.2,89.3,15.2,86,0.2,2.7/0.7,31,13,58.4/57.1,57.5/13.8,5.02,-99 Mexico,CentralAmerica,1964375,129163,66.4,99.2,1140724,2.5,8980.9,3.6,36.0,60.4,13.4,25.2,61.3,4.1,45.5/79.5,120,120,373883,387064,-13181,-33216,1.4,79.2,1.6,2.3,78.9/74.0,26.7/10.1,1193.2/0.9,5.9,18.8,6.3,2.1,5.3,103.2/103.6,93.5/87.7,30.0/29.9,42.6,85.3,57.4,1162,34.0,480.3/3.8,8514,62,97.2/92.1,88.0/74.5,0.03,-99 Moldova,EasternEurope,33846,4051,123.3,92.2,6475,-0.7,1591.4,13.4,20.8,65.8,28.8,30.9,40.3,5.0,39.1/46.5,109,109,2045,4020,-1975,-415,-0.1,45.0,-0.7,1.3,75.2/66.7,15.7/17.6,142.9/3.5,5.5,14.3,10.3,2.5,7.5,91.9/92.9,86.5/85.8,47.4/35.3,22.8,108.0,49.8,35,12.3,4.9/1.2,15,23,96.9/81.4,87.8/67.1,4.49,-99 Mongolia,EasternAsia,1564116,3076,2.0,97.9,11758,2.3,3973.4,14.8,34.1,51.1,28.4,20.7,51.0,6.3,56.8/69.3,147,149,4917,3358,1559,-948,1.9,72.0,2.8,2.8,72.7/64.5,29.7/6.6,17.6/0.6,~0.0,22.8,4.7,2.9,4.6,100.0/101.8,92.4/90.5,79.7/57.7,17.1,105.0,21.4,41,8.1,20.8/7.2,677,84,66.4/59.2,66.4/42.6,2.21,-99 Mozambique,EasternAfrica,799380,29669,37.7,95.5,14806,6.6,529.2,24.6,21.0,54.4,75.0,4.1,21.0,24.1,82.2/75.6,137,137,3352,5295,-1943,-5833,2.9,32.2,3.3,5.4,58.1/54.0,44.8/4.8,222.9/0.8,24.0,67.3,7.0,0.1,6.5,101.2/110.4,31.1/33.8,5.4/7.4,39.6,74.2,9.0,309,48.5,8.4/0.3,779,20,80.6/37.0,42.4/10.1,12.54,-99 Myanmar (Burma),South-easternAsia,676577,53371,81.7,95.5,62601,7.3,1161.5,26.7,34.5,38.7,23.6,14.6,61.7,0.8,74.7/80.8,130,128,11673,15696,-4023,-3921,0.9,34.1,2.5,2.3,68.3/63.7,26.8/9.4,73.3/0.1,1392.1,45.0,2.3,0.6,-99,98.3/101.0,52.0/50.6,14.9/12.1,10.2,76.7,21.8,321,45.3,21.6/0.4,1078,15,92.7/74.4,84.3/77.1,2.23,-99 Namibia,SouthernAfrica,824116,2534,3.1,94.7,11491,5.3,4673.6,6.6,30.6,62.8,29.1,14.6,56.3,24.9,56.4/64.3,88,89,4816,6721,-1905,-1700,2.2,46.7,4.2,3.6,64.3/59.1,36.7/5.5,93.9/3.8,4.8,36.4,-99,...,...,109.5/113.3,.../...,.../...,41.3,102.1,22.3,115,8.5,3.8/1.6,19,31,98.2/84.6,54.5/16.8,1.24,-99 Nepal,SouthernAsia,147181,29305,204.4,94.3,20658,2.7,724.5,31.8,14.9,53.3,72.6,10.9,16.5,3.0,79.7/86.7,139,139,703,5249,-4547,2447,1.2,18.6,3.2,2.3,70.4/67.4,30.9/8.8,518.3/1.8,30.7,32.8,5.8,...,3.7,141.2/130.7,72.2/67.1,15.1/14.8,29.6,96.8,17.6,104,25.4,8.0/0.3,451,19,90.9/91.8,56.0/43.5,5.73,-99 Nicaragua,CentralAmerica,130373,6218,51.7,97.2,12693,4.9,2086.9,18.8,26.8,54.4,24.1,14.5,61.4,6.1,49.6/80.6,130,130,2225,5927,-3701,-1045,1.2,58.8,2.0,2.3,77.5/71.4,29.0/8.4,40.3/0.7,0.6,20.0,9.0,0.9,...,.../...,.../...,.../...,45.7,116.1,19.7,144,25.9,4.9/0.8,91,26,99.3/69.4,76.5/55.7,3.68,-99 Nigeria,WesternAfrica,923768,190886,209.6,102.7,494583,2.7,2714.5,20.9,20.4,58.8,27.9,14.7,57.4,5.4,48.5/64.3,116,117,104084,36533,67551,-15763,2.7,47.8,4.7,5.7,52.6/51.2,44.0/4.5,1199.1/0.7,2437.5,76.3,3.7,...,-99,92.8/94.5,53.5/57.8,8.3/11.8,5.6,82.2,47.4,361,8.1,96.3/0.6,10851,32,80.8/57.3,32.8/25.4,0.52,-99 Pakistan,SouthernAsia,796095,197016,255.6,105.6,266458,5.5,1410.4,25.5,19.0,55.5,42.1,19.8,38.1,5.9,24.8/82.5,135,138,20534,46998,-26464,-1603,2.1,38.8,2.8,3.7,66.8/65.0,34.8/6.7,3629.0/1.9,2739.4,69.8,2.6,0.8,2.6,85.2/99.7,39.2/49.5,9.2/10.6,20.6,66.9,18.0,140,2.0,166.3/0.9,2202,17,93.9/89.9,83.1/51.1,1.32,-99 Palestine,WesternAsia,6020,4921,817.4,102.9,12677,3.5,2715.5,4.8,23.4,71.9,8.7,29.8,61.5,24.2,18.4/69.7,90,90,929,5058,-4128,-1713,2.7,75.3,2.8,4.2,74.8/71.1,39.6/4.6,255.5/5.5,~0.0,20.0,-99,-99,1.3,94.3/94.4,87.0/79.2,54.4/34.5,-99,77.6,57.4,31,1.5,2.8/0.6,9,15,50.7/81.5,93.0/90.2,17.52,-99 Panama,CentralAmerica,75320,4099,55.1,100.4,52132,5.8,13268.1,2.8,27.1,70.0,14.5,19.6,65.9,6.2,50.5/80.4,117,118,636,11697,-11061,-3377,1.7,66.6,2.1,2.6,80.5/74.3,27.4/11.4,184.7/4.7,21.5,15.2,8.0,1.6,3.2,100.9/103.5,78.3/72.9,46.5/31.2,18.3,174.2,51.2,383,62.3,8.8/2.3,31,35,97.7/88.6,83.5/58.0,0.02,-99 Paraguay,SouthAmerica,406752,6811,17.1,102.9,27714,3.1,4174.4,19.0,29.5,51.6,19.6,19.5,61.0,5.5,58.3/84.7,160,166,8494,9753,-1259,-462,1.3,59.7,2.1,2.6,74.9/70.7,29.4/9.4,156.5/2.4,0.2,28.8,9.8,1.3,5.0,104.3/107.6,79.1/74.2,.../...,13.8,105.4,44.4,59,39.4,5.7/0.9,325,38,100.0/94.9,95.5/78.4,0.22,-99 Peru,SouthAmerica,1285216,32166,25.1,99.8,190428,3.3,6069.1,7.6,33.3,59.2,24.4,17.3,58.3,5.3,66.1/82.8,145,148,36040,36185,-145,-9210,1.3,78.6,1.7,2.5,76.8/71.5,27.4/10.4,90.9/0.3,2.5,18.6,5.5,1.1,4.0,101.7/101.7,95.8/95.7,.../...,27.7,109.9,40.9,685,57.9,61.7/2.0,1020,30,91.4/69.2,82.5/53.2,0.18,-99 Philippines,South-easternAsia,300000,104918,351.9,101.3,292449,5.9,2904.2,10.5,31.3,58.2,27.7,16.3,56.1,5.9,50.8/78.9,122,120,56313,85909,-29596,7694,1.6,44.4,1.3,3.0,72.1/65.4,31.7/7.6,211.9/0.2,240.2,22.2,4.7,...,...,116.9/116.8,92.7/84.4,40.3/31.4,29.5,118.1,40.7,783,26.2,105.7/1.1,991,19,93.7/90.3,77.9/70.8,0.15,-99 Puerto Rico,Caribbean,8868,3663,413.0,92.6,102906,-~0.0,27939.0,0.8,50.0,49.1,2.0,17.5,80.5,12.8,34.4/51.7,110,111,-99,-99,-99,-99,-0.2,93.6,-0.2,1.5,83.2/75.2,17.9/20.4,275.0/7.5,-99,6.3,-99,-99,6.4,90.4/89.1,83.9/78.8,98.8/70.1,-99,87.1,79.5,126,55.5,-99,1,16,.../...,99.3/99.3,-99,-99 Rwanda,EasternAfrica,26338,12208,494.9,96.2,8096,6.9,697.3,34.6,15.1,50.3,75.0,7.2,17.8,2.4,86.1/83.3,139,139,622,1778,-1157,-1099,2.5,28.8,6.4,4.2,67.1/63.1,40.1/4.9,441.5/3.8,155.1,44.0,7.5,...,3.6,133.4/131.7,38.2/35.2,6.9/9.1,61.3,70.5,18.0,62,19.2,0.8/0.1,84,8,86.6/71.9,58.5/62.9,13.67,-99 Saint Vincent and the Grenadines,Caribbean,389,110,281.8,101.7,738,1.6,6739.2,7.5,17.2,75.3,22.1,16.1,61.8,19.1,56.4/76.9,115,115,47,335,-288,-210,~0.0,50.6,0.7,2.0,74.9/70.7,23.8/11.7,4.6/4.2,-99,16.5,8.6,...,...,103.2/105.8,105.0/107.9,-99,13.0,103.6,51.8,58,69.2,0.2/1.9,0,29,95.1/95.1,.../...,1.80,-99 Samoa,Polynesia,2842,196,69.4,106.6,774,2.8,4006.0,9.3,24.2,66.6,5.3,14.8,79.8,6.5,23.3/58.2,107,107,56,350,-294,-44,0.8,19.1,-0.2,4.2,77.4/71.1,36.6/8.5,4.9/2.6,~0.0,18.0,7.2,...,...,106.7/106.7,89.5/80.7,.../...,10.0,58.5,25.4,93,60.4,0.2/1.0,2,24,97.5/99.3,93.3/91.1,12.75,-99 Senegal,WesternAfrica,196712,15851,82.3,96.6,13633,6.5,901.1,15.5,24.1,60.4,51.4,20.7,27.9,9.3,45.3/70.5,126,127,2640,5478,-2838,-1348,3.0,43.7,3.6,5.0,67.5/63.8,42.9/4.7,263.2/1.7,17.6,43.9,4.7,...,7.4,86.9/77.6,49.1/50.2,7.8/12.9,42.7,100.0,21.7,123,43.2,8.9/0.6,77,11,92.9/67.3,65.4/33.8,6.49,-99 Sierra Leone,WesternAfrica,72300,7557,104.7,98.1,4483,-20.3,694.8,51.4,17.6,31.1,68.0,6.5,25.5,3.1,65.1/68.8,168,168,466,958,-492,-1317,2.3,39.9,2.7,4.8,50.7/49.6,42.1/4.2,91.2/1.4,0.8,94.4,11.1,...,2.7,128.2/127.0,40.1/46.5,.../...,12.4,89.5,2.5,177,41.3,1.3/0.2,53,11,84.9/47.8,22.8/6.9,21.56,-99 Solomon Islands,Melanesia,28896,611,21.8,103.4,1075,3.2,1841.6,28.2,15.6,56.3,48.1,2.4,49.5,31.4,61.1/73.5,118,118,437,454,-17,-36,2.1,22.3,4.2,4.1,71.1/68.3,38.8/5.4,2.6/0.4,~0.0,30.0,5.1,0.2,...,113.6/115.0,47.0/49.8,-99,2.0,72.7,10.0,245,78.3,0.2/0.4,3,11,93.2/77.2,81.4/15.0,16.47,-99 Somalia,EasternAfrica,637657,14742,23.5,99.3,1559,2.7,144.5,60.2,7.4,32.5,72.0,4.8,23.2,6.6,33.3/75.9,112,112,925,530,394,-99,2.9,39.6,4.1,6.6,56.5/53.3,46.4/4.4,25.3/0.2,1168.4,79.5,-99,~0.0,-99,.../...,.../...,-99,24.2,52.5,1.8,175,10.3,0.6/0.1,129,12,69.6/8.8,52.0/6.3,22.85,-99 South Africa,SouthernAfrica,1221037,56717,46.8,96.4,314571,1.3,5773.0,2.4,28.9,68.7,6.1,26.2,67.7,26.0,46.4/61.1,125,126,74111,74744,-633,-13644,1.4,64.8,1.6,2.6,63.0/56.1,29.0/8.4,3142.5/5.8,1201.9,36.5,8.8,0.8,6.0,97.3/102.2,111.5/88.0,23.3/15.7,42.2,159.3,51.9,581,7.6,489.8/9.1,7102,122,99.6/81.4,69.6/60.5,0.47,-99 South Sudan,EasternAfrica,658841,12576,20.6,100.4,13167,2.0,1067.0,4.6,58.0,37.4,-99,-99,-99,...,.../...,-99,-99,2389,750,1640,-935,3.3,18.8,5.1,5.2,56.0/54.1,41.7/5.1,824.1/6.7,2231.2,77.7,2.7,-99,1.8,53.1/74.9,6.6/12.3,-99,28.5,23.9,17.9,49,-99,1.5/0.1,337,2,66.7/56.9,16.4/4.5,21.07,-99 Suriname,SouthAmerica,163820,563,3.6,100.7,4879,-2.7,8985.3,11.4,27.4,61.1,3.4,22.3,74.3,9.9,40.5/68.6,152,152,1437,1244,193,-808,1.0,66.0,0.8,2.5,74.2/67.8,26.4/10.4,46.8/8.6,~0.0,17.4,5.7,...,-99,121.4/124.2,90.9/71.7,.../...,25.5,180.7,42.8,83,98.3,2.0/3.7,44,62,98.1/88.4,88.4/61.4,0.32,-99 Tajikistan,CentralAsia,142600,8921,63.7,100.9,7853,4.2,925.9,25.0,28.0,47.1,57.2,13.4,29.4,10.8,59.6/77.9,155,172,899,3030,-2132,-472,2.2,26.8,2.6,3.5,73.5/67.7,35.3/5.8,275.1/3.2,21.8,38.9,6.9,1.7,5.2,101.2/99.8,83.1/92.5,24.0/33.6,19.0,98.6,19.0,45,3.0,5.2/0.6,76,14,93.1/66.7,93.8/95.5,4.55,-99 Tanzania,EasternAfrica,947303,57310,64.7,97.8,45628,7.0,877.3,31.1,26.1,42.9,66.9,6.4,26.7,2.7,73.9/83.2,176,180,4742,7876,-3134,-3312,3.1,31.6,5.4,5.2,64.8/60.8,44.9/4.7,261.2/0.5,402.1,44.0,5.6,~0.0,3.5,82.9/80.5,30.8/33.7,2.5/4.9,36.4,75.9,5.4,1082,52.4,11.6/0.2,935,20,77.2/45.5,31.3/8.3,5.85,-99 Thailand,South-easternAsia,513120,69038,135.1,95.2,395168,2.8,5814.8,9.1,35.7,55.1,34.0,22.7,43.3,0.6,62.7/79.8,129,126,213927,195666,18260,32149,0.4,50.4,3.0,1.5,78.4/70.8,17.3/16.9,3913.3/5.8,554.1,11.2,4.1,...,4.1,99.2/106.1,125.3/132.6,57.3/40.5,4.9,125.8,39.3,611,32.0,316.2/4.7,3338,83,97.6/98.0,89.9/96.1,0.02,-99 The Democratic Republic of the Congo,MiddleAfrica,2344858,81340,35.9,99.6,37569,7.0,486.2,19.9,44.2,35.9,65.3,5.9,28.9,3.6,70.4/71.8,104,104,5103,5906,-803,-1546,3.3,42.5,4.0,6.4,59.5/56.7,46.3/4.7,545.7/0.7,2163.3,73.2,4.3,...,2.2,101.8/112.0,33.3/53.6,4.2/9.1,8.9,53.0,3.8,349,67.4,4.7/0.1,1179,16,81.1/31.2,28.5/28.7,8.01,-99 Timor-Leste,South-easternAsia,14919,1296,87.2,103.2,2873,4.3,2425.4,5.2,79.9,14.9,50.8,2.0,47.2,4.3,26.9/55.6,111,117,94,647,-553,238,2.2,32.8,3.8,5.9,69.5/66.1,43.6/5.4,10.8/0.9,~0.0,43.9,1.5,0.1,7.8,136.2/137.5,79.6/74.1,.../...,38.5,117.4,13.4,24,46.9,0.5/0.4,143,7,95.2/60.5,69.0/26.8,8.89,-99 Togo,WesternAfrica,56785,7798,143.4,99.4,4086,5.5,559.4,45.7,19.7,34.6,62.5,8.7,28.7,6.8,81.0/80.7,140,143,715,1716,-1001,-461,2.6,40.0,3.8,4.7,59.8/58.3,41.6/4.6,276.8/3.8,13.8,55.7,5.2,...,5.2,118.5/125.1,.../...,6.4/14.9,17.6,65.0,7.1,80,3.8,2.6/0.4,111,20,91.4/44.2,24.7/2.9,5.51,-99 Turkey,WesternAsia,783562,80745,104.9,97.0,717888,4.0,9125.8,8.6,26.4,65.0,19.6,27.5,52.9,10.8,30.4/71.4,120,122,142606,198602,-55996,-32278,1.6,73.4,2.0,2.1,78.1/71.5,25.0/12.0,2964.9/3.8,3006.3,12.6,5.4,1.7,4.8,102.1/102.8,101.1/103.8,88.3/101.0,14.9,96.0,53.7,388,15.1,346.0/4.5,1303,65,100.0/100.0,98.3/85.5,0.30,-99 Uganda,EasternAfrica,241550,42863,214.5,99.0,25282,5.4,647.7,25.6,21.5,52.9,72.0,7.4,20.5,2.4,82.3/87.7,93,90,2755,3750,-996,-2353,3.4,16.1,5.4,5.9,60.7/56.5,47.7/3.3,749.5/1.9,727.1,60.2,7.2,...,2.2,110.9/108.9,22.1/24.3,4.2/5.4,34.3,50.4,19.2,196,11.0,5.2/0.1,409,12,95.5/75.8,28.5/17.3,6.35,-99 Ukraine,EasternEurope,603500,44223,76.3,86.0,90615,-9.9,2021.6,14.0,26.3,59.7,15.7,24.6,59.7,8.8,52.3/67.5,137,137,36369,39184,-2815,-189,-0.5,69.7,-0.3,1.5,76.0/66.1,15.5/23.2,4834.9/10.8,1644.8,8.8,7.1,3.0,5.9,105.1/102.8,98.2/100.2,88.4/76.5,12.3,144.0,49.3,102,16.6,227.3/5.1,3203,98,95.5/97.8,97.4/92.6,1.63,-99 United States,NorthernAmerica,9833517,324460,35.5,98.0,18036648,2.6,56053.8,1.0,19.7,79.3,1.5,17.2,81.3,4.9,55.7/68.1,111,113,1453167,2249661,-796494,-462961,0.7,81.6,1.0,1.9,81.2/76.5,18.9/21.5,46627.1/14.5,616.5,6.0,17.1,2.6,5.4,100.0/100.3,98.5/96.7,99.6/72.8,19.1,117.6,74.6,1513,33.9,5254.3/16.2,83887,289,99.4/98.2,100.0/100.0,-99,-99 Vanuatu,Melanesia,12189,276,22.7,102.4,737,-1.0,2783.0,26.7,8.4,64.9,61.4,6.8,31.8,5.3,61.7/80.5,123,122,50,416,-366,-82,2.3,26.1,3.4,3.4,73.6/69.4,35.9/6.7,3.2/1.2,~0.0,24.3,5.0,0.2,5.5,118.7/120.6,56.4/53.4,.../...,0.0,66.2,22.4,137,36.1,0.2/0.6,1,12,98.9/92.9,65.1/55.4,12.32,-99 Vietnam,South-easternAsia,330967,95541,308.1,98.0,193241,6.7,2067.9,18.9,37.0,44.2,41.8,22.9,35.2,2.2,73.9/83.3,136,134,176632,174111,2520,906,1.1,33.6,3.0,2.0,80.3/70.7,23.1/11.1,72.8/0.1,11.0,19.3,7.1,1.2,5.7,108.4/109.3,-99,28.9/28.8,26.7,130.6,52.7,616,47.2,166.9/1.8,2977,30,99.1/96.9,94.4/69.7,1.73,-99 Virgin Islands,Caribbean,347,105,299.7,91.1,-99,-99,-99,-99,-99,-99,10.9,24.0,65.2,9.0,52.5/70.7,109,109,-99,-99,-99,-99,-0.2,95.3,0.3,2.3,81.5/76.7,20.1/25.3,56.7/53.4,-99,9.3,-99,-99,-99,-99,-99,-99,-99,...,54.8,58,50.6,-99,-99,-99,100.0/100.0,96.4/96.4,-99,-99 Yemen,WesternAsia,527968,28250,53.5,102.1,29688,-28.1,1106.4,14.7,36.9,48.4,32.9,17.9,49.2,16.1,26.2/73.7,136,137,570,6861,-6291,-3026,2.6,34.6,4.0,4.4,65.6/62.8,39.9/4.6,344.1/1.3,3371.4,47.2,5.6,0.3,...,88.9/105.7,39.5/57.4,6.1/13.7,0.0,68.0,25.1,298,1.0,22.7/0.9,668,12,72.0/46.5,92.5/34.1,2.99,-99 Zambia,EasternAfrica,752612,17094,23.0,98.5,21255,2.9,1311.1,8.2,32.3,59.5,54.8,9.9,35.3,7.4,69.9/80.9,179,180,6505,7442,-937,-768,3.0,40.9,4.3,5.2,61.9/57.5,44.8/3.7,127.9/0.8,55.3,53.8,5.0,0.2,...,104.0/103.3,-99,3.4/4.5,18.0,74.5,21.0,88,65.6,4.5/0.3,374,26,85.6/51.3,55.6/35.7,3.96,-99 Zimbabwe,EasternAfrica,390757,16530,42.7,95.0,13893,1.1,890.4,13.0,30.5,56.5,67.5,7.3,25.2,5.0,78.0/87.5,99,98,2832,5212,-2379,-1521,2.3,32.4,2.3,4.0,59.0/56.1,41.2/4.2,398.9/2.6,308.6,46.5,6.0,0.1,8.4,99.1/100.8,47.1/48.1,8.0/8.9,32.6,84.8,16.4,89,37.2,12.0/0.8,482,30,97.0/67.3,49.3/30.8,6.00,-99 ================================================ FILE: intro_data_science/part_4/ds_part_4.ipynb ================================================ { "metadata": { "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" }, "orig_nbformat": 2, "kernelspec": { "name": "python3", "display_name": "Python 3", "language": "python" } }, "nbformat": 4, "nbformat_minor": 2, "cells": [ { "source": [ "# A Brief Introduction to Pandas\n", "### Part 2" ], "cell_type": "markdown", "metadata": {} }, { "source": [ "## 3.1 Selection\n", "Using .loc(), .iloc()\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.loc.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "import pandas as pd\n", "import matplotlib.pyplot as plt\n", "import numpy as np\n", "from scipy import stats" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Read in the complete version of the europe data, using the first column as the index\n", "eur_data_final = pd.read_csv('./data/complete/eur_data_final.csv', index_col=0)\n", "eur_data_final.head()" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Data from the eur_data_final df, represented as a python dictionary\n", "countries_dict = {\n", " 15: {\n", " 'country': 'Italy', \n", " 'unemp_rate': 11.7, \n", " 'gdp': 1689824, \n", " 'median_income': 16237, \n", " 'total_pop': 59433744\n", " }\n", "}" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# With vanilla python, how do we get the word 'Italy' from a dictionary?" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# How do we do this with a dataframe?" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# We can also get multiple columns" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Or an entire row/entry" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Or multiple rows and columns" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# We can also use python's index slicing syntax" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Select by column value (Pandas is smart!)\n", "# DF must be indexed by country name" ] }, { "source": [ "### Exercise - Use .loc() to create a new dataframe with all countries from Cypress to France (alphabetically) with gdp and total_pop columns." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] }, { "source": [ "### Exercise - What countries have a higher unemployment rate than Slovenia and have a lowercase 't' in their name?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Select slovenia unemployment values\n", "\n", "# Generate comparison query\n", "\n", "# Generate 'contains' query\n", "\n", "# Make selection using queries\n" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Explore .iloc()\n", "# https://stackoverflow.com/questions/31593201/how-are-iloc-and-loc-different\n" ] }, { "source": [ "## 4.1 MultiIndexes (hierarchical indexes)\n", "Pandas also supports multindexes, which allow users to index by multiple values or groups of values.\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.MultiIndex.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "parent_array = ['bar', 'bar', 'baz', 'baz', 'foo', 'foo', 'qux', 'qux']\n", "child_array = ['one', 'two', 'one', 'two', 'one', 'two', 'one', 'two']\n", "# Add arrays to one array" ] }, { "source": [ "We want a multidimensional array of random numbers. Numpy for the win!" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Set the seed for number generation\n", "np.random.seed(42)\n", "# Create multidimensional array of pseudorandom numbers with shape 8,4" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Convert md array to dataframe\n", "multi_df = pd.DataFrame(md_array)\n", "\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.RangeIndex.html\n", "multi_df.index" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Add multindex\n", "multi_df = pd.DataFrame(md_array, index=arrays)\n", "multi_df.index" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Update column names of multi_df\n", "col_names = ['var1', 'var2', 'var3', 'var4']\n", "multi_df.columns = col_names" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Select all columns and rows for 'bar'" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Select all rows for index foo and column var1\n", "# Using loc, fancy indexing\n", "\n", "# # Using loc, bracket notation\n", "\n", "# # Using loc, dot notation\n", "\n", "# # Without loc\n" ] }, { "source": [ "## 4.2 Groupby\n", "From the documentation: \"A groupby operation involves some combination of splitting the object, applying a function, and combining the results.\"\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.groupby.html\n", "\n", "This is tied closely to the split-apply-combine strategy: https://pandas.pydata.org/pandas-docs/stable/user_guide/groupby.html. This was approach outlined by Hadley Wickcham in this paper: https://www.jstatsoft.org/article/view/v040i01.\n" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Read in the UN world data\n", "un_data = pd.read_csv('./data/complete/un_world_data.csv')" ] }, { "source": [ "This data is wide! Let's get rid of some of the columns." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# This data is wide! Let's get rid of some of the columns.\n", "columns_to_keep = [\n", " 'country',\n", " 'Region',\n", " 'Surface area (km2)',\n", " 'GDP: Gross domestic product (million current US$)', \n", " 'Population in thousands (2017)', \n", " 'Population density (per km2, 2017)'\n", " ]" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Rename the columns \n", "columns = {\n", " \"Surface area (km2)\": \"\", \n", " \"GDP: Gross domestic product (million current US$)\": \"\",\n", " \"Population in thousands (2017)\": \"\",\n", " \"Population density (per km2, 2017)\": ''\n", " }" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Group the data by region\n", "un_region = un_data.groupby('Region', as_index=False)\n", "# The groupby object is iterable" ] }, { "source": [ "Note: generally speaking, you want to avoid iteration with Pandas. It's best to leverage the power of vectorized operations. If you find yourself looping through a dataframe or a series, you might be doing unecessary work. https://towardsdatascience.com/you-dont-always-have-to-loop-through-rows-in-pandas-22a970b347ac" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Select Caribbean group\n", "carib = un_region.get_group('Caribbean')\n", "\n", "# Get the head and tail" ] }, { "source": [ "### Exercise - Get the average surface area of all the countries in CentralAmerica" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] }, { "source": [ "We can do vectorized operations on each group object." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# .min(), .max(), .mean(), .count()" ] }, { "source": [ "Let's take a look at aggregating the data using the .agg() method: https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.agg.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "\n", "# Get the mean of each column for each region" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Get mean, median, and sum" ] }, { "source": [ "We can also pass in other functions or define our own. These are referred to as higher-order functions, i.e. functions that take in other functions as arguments. \n", "\n" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Example of higher-order function\n", "def do_calculation(val, func):\n", " return func(val)" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Example of filtering" ] }, { "source": [ "Let's use the statistics module from scipy to calculate some new values: https://docs.scipy.org/doc/scipy/reference/stats.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# stats.tsem(), stats.tstd(), stats.skew()" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Use a lambda function\n", "# https://www.geeksforgeeks.org/python-lambda-anonymous-functions-filter-map-reduce/" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Get the max of each column in each region" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Select a single column" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Get sum and mean of surface area, mean of population for each region" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Define custom column names" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# .filter()\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.DataFrameGroupBy.filter.html\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/groupby.html" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Filter by population" ] }, { "source": [ "### Exercise - Get standard deviation of gdp for each region using np.std for all regions with a population density over 100" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] }, { "source": [ "### Exercise - Generate corr plot for the UN data" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Select columns\n", "\n", "# Make subplot and figure\n", "\n", "# Generate correlation matrix\n", "\n", "# Generate matplotlib plot\n", "\n", "# Add colorbar to figure\n", "\n", "# Set tick labels" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] } ] } ================================================ FILE: intro_data_science/part_4/ds_part_4_complete.ipynb ================================================ { "metadata": { "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.2-final" }, "orig_nbformat": 2, "kernelspec": { "name": "python3", "display_name": "Python 3", "language": "python" } }, "nbformat": 4, "nbformat_minor": 2, "cells": [ { "source": [ "# A Brief Introduction to Pandas\n", "### Part 2" ], "cell_type": "markdown", "metadata": {} }, { "source": [ "## 3.1 Selection\n", "Using .loc(), .iloc()\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.loc.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 3, "metadata": {}, "outputs": [], "source": [ "import pandas as pd\n", "import matplotlib.pyplot as plt\n", "import numpy as np\n", "from scipy import stats" ] }, { "cell_type": "code", "execution_count": 201, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "0 Austria 6.0 356237.6 23071 8401940\n", "1 Belgium 7.8 424660.3 21335 11000638\n", "2 Bulgaria 7.6 48128.6 6742 7364570\n", "3 Croatia 13.1 46639.5 8985 4284889\n", "4 Cyprus 13.0 18490.2 16173 840407" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
0Austria6.0356237.6230718401940
1Belgium7.8424660.32133511000638
2Bulgaria7.648128.667427364570
3Croatia13.146639.589854284889
4Cyprus13.018490.216173840407
\n
" }, "metadata": {}, "execution_count": 201 } ], "source": [ "# Read in the complete version of the europe data, using the first column as the index\n", "eur_data_final = pd.read_csv('./data/complete/eur_data_final.csv', index_col=0)\n", "eur_data_final.head()" ] }, { "cell_type": "code", "execution_count": 132, "metadata": {}, "outputs": [], "source": [ "# Data from the eur_data_final df, represented as a python dictionary\n", "countries_dict = {\n", " 15: {\n", " 'country': 'Italy', \n", " 'unemp_rate': 11.7, \n", " 'gdp': 1689824, \n", " 'median_income': 16237, \n", " 'total_pop': 59433744\n", " }\n", "}" ] }, { "cell_type": "code", "execution_count": 133, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "'Italy'" ] }, "metadata": {}, "execution_count": 133 } ], "source": [ "# With vanilla python, how do we get the word 'Italy' from a dictionary?\n", "countries_dict[15]['country']" ] }, { "cell_type": "code", "execution_count": 134, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "'Italy'" ] }, "metadata": {}, "execution_count": 134 } ], "source": [ "# How do we do this with a dataframe?\n", "eur_data_final.loc[15, 'country']" ] }, { "cell_type": "code", "execution_count": 136, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "country Italy\n", "unemp_rate 11.7\n", "gdp 1689824.0\n", "median_income 16237\n", "Name: 15, dtype: object" ] }, "metadata": {}, "execution_count": 136 } ], "source": [ "# We can also get multiple columns\n", "eur_data_final.loc[15, ['country', 'gdp']]" ] }, { "cell_type": "code", "execution_count": 9, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "country Hungary\n", "unemp_rate 5.1\n", "gdp 113903.8\n", "median_income 8267\n", "total_pop 9937628\n", "Name: 12, dtype: object" ] }, "metadata": {}, "execution_count": 9 } ], "source": [ "# Or an entire row/entry\n", "eur_data_final.loc[12]" ] }, { "cell_type": "code", "execution_count": 10, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country gdp\n", "9 France 2228568.0\n", "10 Germany 3159750.0" ], "text/html": "
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countrygdp
9France2228568.0
10Germany3159750.0
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" }, "metadata": {}, "execution_count": 10 } ], "source": [ "# Or multiple rows and columns\n", "eur_data_final.loc[[9,10], ['country','gdp']]" ] }, { "cell_type": "code", "execution_count": 137, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income\n", "9 France 10.1 2228568.0 20621\n", "10 Germany 4.1 3159750.0 21152\n", "11 Greece 23.6 176487.9 9048\n", "12 Hungary 5.1 113903.8 8267\n", "13 Iceland 3.0 18646.1 22193\n", "14 Ireland 8.4 273238.2 18286" ], "text/html": "
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countryunemp_rategdpmedian_income
9France10.12228568.020621
10Germany4.13159750.021152
11Greece23.6176487.99048
12Hungary5.1113903.88267
13Iceland3.018646.122193
14Ireland8.4273238.218286
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" }, "metadata": {}, "execution_count": 137 } ], "source": [ "# We can also use python's index slicing syntax\n", "eur_data_final.loc[9:14, 'country':'median_income']" ] }, { "cell_type": "code", "execution_count": 11, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "unemp_rate 8.0\n", "gdp 40357.2\n", "median_income 15250.0\n", "total_pop 2050189.0\n", "Name: Slovenia, dtype: float64" ] }, "metadata": {}, "execution_count": 11 } ], "source": [ "# Select by column value (Pandas is smart!)\n", "# DF must be indexed by country name\n", "eur_data_country_index = eur_data_final.sort_values('country').set_index('country')\n", "eur_data_country_index.loc['Slovenia']" ] }, { "source": [ "### Exercise - Use .loc() to create a new dataframe with all countries from Cypress to France (alphabetically) with gdp and total_pop columns." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 144, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " gdp total_pop\n", "country \n", "Cyprus 18490.2 840407\n", "Czechia 176370.1 10436560\n", "Denmark 282089.9 5560628\n", "Estonia 21682.6 1294455\n", "Finland 216073.0 5375276\n", "France 2228568.0 64933400" ], "text/html": "
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gdptotal_pop
country
Cyprus18490.2840407
Czechia176370.110436560
Denmark282089.95560628
Estonia21682.61294455
Finland216073.05375276
France2228568.064933400
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" }, "metadata": {}, "execution_count": 144 } ], "source": [ "eur_data_country_index.loc['Cypress': 'France', ['gdp', 'total_pop']]" ] }, { "source": [ "### Exercise - What countries have a higher unemployment rate than Slovenia and have a lowercase 't' in their name?" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 20, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country unemp_rate gdp median_income total_pop\n", "3 Croatia 13.1 46639.5 8985 4284889\n", "15 Italy 11.7 1689824.0 16237 59433744\n", "16 Latvia 9.6 25037.7 9257 2070371\n", "23 Portugal 11.2 186480.5 10805 10562178" ], "text/html": "
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countryunemp_rategdpmedian_incometotal_pop
3Croatia13.146639.589854284889
15Italy11.71689824.01623759433744
16Latvia9.625037.792572070371
23Portugal11.2186480.51080510562178
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" }, "metadata": {}, "execution_count": 20 } ], "source": [ "# Select slovenia unemployment values\n", "slovenia_unemployment = eur_data_country_index.loc['Slovenia', 'unemp_rate']\n", "# Generate comparison query\n", "gt_slov = eur_data_final.unemp_rate > slovenia_unemployment\n", "# Generate 'contains' query\n", "t_names = eur_data_final.country.str.contains('t')\n", "# Make selection using queries\n", "eur_data_final[gt_slov & t_names]" ] }, { "cell_type": "code", "execution_count": 14, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "country Austria\n", "unemp_rate 6.0\n", "gdp 356237.6\n", "median_income 23071\n", "total_pop 8401940\n", "Name: 0, dtype: object" ] }, "metadata": {}, "execution_count": 14 } ], "source": [ "# Explore .iloc()\n", "# https://stackoverflow.com/questions/31593201/how-are-iloc-and-loc-different\n", "eur_data_final.iloc[0]" ] }, { "source": [ "## 4.1 MultiIndexes (hierarchical indexes)\n", "Pandas also supports multindexes, which allow users to index by multiple values or groups of values.\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.MultiIndex.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 5, "metadata": {}, "outputs": [], "source": [ "parent_array = ['bar', 'bar', 'baz', 'baz', 'foo', 'foo', 'qux', 'qux']\n", "child_array = ['one', 'two', 'one', 'two', 'one', 'two', 'one', 'two']\n", "arrays = [parent_array, child_array]" ] }, { "source": [ "We want a multidimensional array of random numbers. Numpy for the win!" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 7, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "array([[6, 3, 7, 4],\n", " [6, 9, 2, 6],\n", " [7, 4, 3, 7],\n", " [7, 2, 5, 4],\n", " [1, 7, 5, 1],\n", " [4, 0, 9, 5],\n", " [8, 0, 9, 2],\n", " [6, 3, 8, 2]])" ] }, "metadata": {}, "execution_count": 7 } ], "source": [ "# Set the seed for number generation\n", "np.random.seed(42)\n", "# Create multidimensional array with shape 8,4\n", "md_array = np.random.randint(10,size=(8,4))\n", "md_array" ] }, { "cell_type": "code", "execution_count": 10, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "RangeIndex(start=0, stop=8, step=1)" ] }, "metadata": {}, "execution_count": 10 } ], "source": [ "# Convert md array to dataframe\n", "multi_df = pd.DataFrame(md_array)\n", "\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.RangeIndex.html\n", "multi_df.index" ] }, { "cell_type": "code", "execution_count": 12, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "MultiIndex([('bar', 'one'),\n", " ('bar', 'two'),\n", " ('baz', 'one'),\n", " ('baz', 'two'),\n", " ('foo', 'one'),\n", " ('foo', 'two'),\n", " ('qux', 'one'),\n", " ('qux', 'two')],\n", " )" ] }, "metadata": {}, "execution_count": 12 } ], "source": [ "# Add multindex\n", "multi_df = pd.DataFrame(md_array, index=arrays)\n", "multi_df.index" ] }, { "cell_type": "code", "execution_count": 14, "metadata": {}, "outputs": [], "source": [ "# Update column names of multi_df\n", "multi_df.columns = ['var1', 'var2', 'var3', 'var4']" ] }, { "cell_type": "code", "execution_count": 18, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " var1 var2 var3 var4\n", "one 6 3 7 4\n", "two 6 9 2 6" ], "text/html": "
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var1var2var3var4
one6374
two6926
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" }, "metadata": {}, "execution_count": 18 } ], "source": [ "# Select all columns and rows for 'bar'\n", "multi_df.loc['bar']" ] }, { "cell_type": "code", "execution_count": 34, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "one 1\n", "two 4\n", "Name: var1, dtype: int64" ] }, "metadata": {}, "execution_count": 34 } ], "source": [ "# Select all rows for index foo and column var1\n", "# Using loc, fancy indexing\n", "multi_df.loc['foo', 'var1']\n", "# # Using loc, bracket notation\n", "# multi_df.loc['foo']['var1']\n", "# # Using loc, dot notation\n", "# multi_df.loc['foo'].var1\n", "# # Without loc\n", "# multi_df.var1.foo.one" ] }, { "source": [ "## 4.2 Groupby\n", "From the documentation: \"A groupby operation involves some combination of splitting the object, applying a function, and combining the results.\"\n", "https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.groupby.html\n", "\n", "This is tied closely to the split-apply-combine strategy: https://pandas.pydata.org/pandas-docs/stable/user_guide/groupby.html. This was approach outlined by Hadley Wickcham in this paper: https://www.jstatsoft.org/article/view/v040i01.\n" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 204, "metadata": {}, "outputs": [], "source": [ "# Read in the UN world data\n", "un_data = pd.read_csv('./data/complete/un_world_data.csv')" ] }, { "source": [ "This data is wide! Let's get rid of some of the columns." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 38, "metadata": {}, "outputs": [], "source": [ "# This data is wide! Let's get rid of some of the columns.\n", "columns_to_keep = [\n", " 'country',\n", " 'Region',\n", " 'Surface area (km2)',\n", " 'GDP: Gross domestic product (million current US$)', \n", " 'Population in thousands (2017)', \n", " 'Population density (per km2, 2017)'\n", " ]\n", "un_data = un_data[columns_to_keep]" ] }, { "cell_type": "code", "execution_count": 43, "metadata": {}, "outputs": [], "source": [ "# Rename the columns \n", "columns = {\n", " \"Surface area (km2)\": \"surface_area\", \n", " \"GDP: Gross domestic product (million current US$)\": \"gdp\",\n", " \"Population in thousands (2017)\": 'population',\n", " \"Population density (per km2, 2017)\": 'population_density'\n", " }\n", "\n", "un_data = un_data.rename(columns=columns)" ] }, { "cell_type": "code", "execution_count": 202, "metadata": {}, "outputs": [], "source": [ "# Group the data by region\n", "un_region = un_data.groupby('Region', as_index=False)\n", "# The groupby object is iterable\n", "for region, region_df in un_region:\n", " print(region)\n", " print(region_df)" ] }, { "source": [ "Note: generally speaking, you want to avoid iteration with Pandas. It's best to leverage the power of vectorized operations. If you find yourself looping through a dataframe or a series, you might be doing unecessary work. https://towardsdatascience.com/you-dont-always-have-to-loop-through-rows-in-pandas-22a970b347ac" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 203, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country Region surface_area gdp \\\n", "19 Dominican Republic Caribbean 48671 67103 \n", "27 Haiti Caribbean 27750 8501 \n", "59 Puerto Rico Caribbean 8868 102906 \n", "61 Saint Vincent and the Grenadines Caribbean 389 738 \n", "82 Virgin Islands Caribbean 347 -99 \n", "\n", " population population_density \n", "19 10767 222.8 \n", "27 10981 398.4 \n", "59 3663 413.0 \n", "61 110 281.8 \n", "82 105 299.7 " ], "text/html": "
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countryRegionsurface_areagdppopulationpopulation_density
19Dominican RepublicCaribbean486716710310767222.8
27HaitiCaribbean27750850110981398.4
59Puerto RicoCaribbean88681029063663413.0
61Saint Vincent and the GrenadinesCaribbean389738110281.8
82Virgin IslandsCaribbean347-99105299.7
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" }, "metadata": {}, "execution_count": 203 } ], "source": [ "# Select Caribbean group\n", "carib = un_region.get_group('Caribbean')\n", "\n", "# Get the head and tail\n", "carib.head()\n", "carib.tail()" ] }, { "source": [ "### Exercise - Get the average surface area of all the countries in CentralAmerica" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 46, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "310819.5" ] }, "metadata": {}, "execution_count": 46 } ], "source": [ "central_america = un_region.get_group('CentralAmerica')\n", "central_america['surface_area'].mean()" ] }, { "source": [ "We can do vectorized operations on each group object." ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 76, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " Region surface_area gdp population \\\n", "0 Caribbean 1.720500e+04 3.582980e+04 5125.200000 \n", "1 CentralAmerica 3.108195e+05 1.712796e+05 22164.750000 \n", "2 CentralAsia 1.712745e+05 7.212500e+03 7483.000000 \n", "3 EasternAfrica 4.816671e+05 1.883158e+04 25645.750000 \n", "4 EasternAsia 5.582058e+06 5.585108e+06 706296.500000 \n", "5 EasternEurope 3.186730e+05 4.854500e+04 24137.000000 \n", "6 Melanesia 2.054250e+04 9.060000e+02 443.500000 \n", "7 Micronesia 5.490000e+02 -9.900000e+01 164.000000 \n", "8 MiddleAfrica 1.054169e+06 2.482600e+04 36885.000000 \n", "9 NorthernAfrica 1.002000e+06 3.159170e+05 97553.000000 \n", "10 NorthernAmerica 9.833517e+06 1.803665e+07 324460.000000 \n", "11 Polynesia 2.842000e+03 7.740000e+02 196.000000 \n", "12 South-easternAsia 5.205436e+05 2.298626e+05 76377.250000 \n", "13 SouthAmerica 1.703150e+06 3.327079e+05 42953.250000 \n", "14 SouthernAfrica 6.918360e+05 1.093567e+05 20494.666667 \n", "15 SouthernAsia 9.843594e+05 4.851398e+05 320367.800000 \n", "16 SouthernEurope 2.874800e+04 1.154100e+04 2930.000000 \n", "17 WesternAfrica 4.164142e+05 5.688491e+04 30298.545455 \n", "18 WesternAsia 2.060487e+05 1.389109e+05 19296.700000 \n", "\n", " population_density \n", "0 323.140000 \n", "1 104.262500 \n", "2 47.600000 \n", "3 139.458333 \n", "4 76.050000 \n", "5 99.800000 \n", "6 22.250000 \n", "7 304.100000 \n", "8 34.066667 \n", "9 98.000000 \n", "10 35.500000 \n", "11 69.400000 \n", "12 153.762500 \n", "13 27.050000 \n", "14 41.166667 \n", "15 197.200000 \n", "16 106.900000 \n", "17 89.181818 \n", "18 243.040000 " ], "text/html": "
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Regionsurface_areagdppopulationpopulation_density
0Caribbean1.720500e+043.582980e+045125.200000323.140000
1CentralAmerica3.108195e+051.712796e+0522164.750000104.262500
2CentralAsia1.712745e+057.212500e+037483.00000047.600000
3EasternAfrica4.816671e+051.883158e+0425645.750000139.458333
4EasternAsia5.582058e+065.585108e+06706296.50000076.050000
5EasternEurope3.186730e+054.854500e+0424137.00000099.800000
6Melanesia2.054250e+049.060000e+02443.50000022.250000
7Micronesia5.490000e+02-9.900000e+01164.000000304.100000
8MiddleAfrica1.054169e+062.482600e+0436885.00000034.066667
9NorthernAfrica1.002000e+063.159170e+0597553.00000098.000000
10NorthernAmerica9.833517e+061.803665e+07324460.00000035.500000
11Polynesia2.842000e+037.740000e+02196.00000069.400000
12South-easternAsia5.205436e+052.298626e+0576377.250000153.762500
13SouthAmerica1.703150e+063.327079e+0542953.25000027.050000
14SouthernAfrica6.918360e+051.093567e+0520494.66666741.166667
15SouthernAsia9.843594e+054.851398e+05320367.800000197.200000
16SouthernEurope2.874800e+041.154100e+042930.000000106.900000
17WesternAfrica4.164142e+055.688491e+0430298.54545589.181818
18WesternAsia2.060487e+051.389109e+0519296.700000243.040000
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" }, "metadata": {}, "execution_count": 76 } ], "source": [ "# .min(), .max(), .mean(), .count()\n", "un_region.min()\n", "un_region.max()\n", "un_region.mean()\n", "un_region.count()" ] }, { "source": [ "Let's take a look at aggregating the data using the .agg() method: https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.agg.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 85, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " surface_area gdp population \\\n", " mean mean mean \n", "Region \n", "Caribbean 1.720500e+04 3.582980e+04 5125.200000 \n", "CentralAmerica 3.108195e+05 1.712796e+05 22164.750000 \n", "CentralAsia 1.712745e+05 7.212500e+03 7483.000000 \n", "EasternAfrica 4.816671e+05 1.883158e+04 25645.750000 \n", "EasternAsia 5.582058e+06 5.585108e+06 706296.500000 \n", "EasternEurope 3.186730e+05 4.854500e+04 24137.000000 \n", "Melanesia 2.054250e+04 9.060000e+02 443.500000 \n", "Micronesia 5.490000e+02 -9.900000e+01 164.000000 \n", "MiddleAfrica 1.054169e+06 2.482600e+04 36885.000000 \n", "NorthernAfrica 1.002000e+06 3.159170e+05 97553.000000 \n", "NorthernAmerica 9.833517e+06 1.803665e+07 324460.000000 \n", "Polynesia 2.842000e+03 7.740000e+02 196.000000 \n", "South-easternAsia 5.205436e+05 2.298626e+05 76377.250000 \n", "SouthAmerica 1.703150e+06 3.327079e+05 42953.250000 \n", "SouthernAfrica 6.918360e+05 1.093567e+05 20494.666667 \n", "SouthernAsia 9.843594e+05 4.851398e+05 320367.800000 \n", "SouthernEurope 2.874800e+04 1.154100e+04 2930.000000 \n", "WesternAfrica 4.164142e+05 5.688491e+04 30298.545455 \n", "WesternAsia 2.060487e+05 1.389109e+05 19296.700000 \n", "\n", " population_density \n", " mean \n", "Region \n", "Caribbean 323.140000 \n", "CentralAmerica 104.262500 \n", "CentralAsia 47.600000 \n", "EasternAfrica 139.458333 \n", "EasternAsia 76.050000 \n", "EasternEurope 99.800000 \n", "Melanesia 22.250000 \n", "Micronesia 304.100000 \n", "MiddleAfrica 34.066667 \n", "NorthernAfrica 98.000000 \n", "NorthernAmerica 35.500000 \n", "Polynesia 69.400000 \n", "South-easternAsia 153.762500 \n", "SouthAmerica 27.050000 \n", "SouthernAfrica 41.166667 \n", "SouthernAsia 197.200000 \n", "SouthernEurope 106.900000 \n", "WesternAfrica 89.181818 \n", "WesternAsia 243.040000 " ], "text/html": "
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surface_areagdppopulationpopulation_density
meanmeanmeanmean
Region
Caribbean1.720500e+043.582980e+045125.200000323.140000
CentralAmerica3.108195e+051.712796e+0522164.750000104.262500
CentralAsia1.712745e+057.212500e+037483.00000047.600000
EasternAfrica4.816671e+051.883158e+0425645.750000139.458333
EasternAsia5.582058e+065.585108e+06706296.50000076.050000
EasternEurope3.186730e+054.854500e+0424137.00000099.800000
Melanesia2.054250e+049.060000e+02443.50000022.250000
Micronesia5.490000e+02-9.900000e+01164.000000304.100000
MiddleAfrica1.054169e+062.482600e+0436885.00000034.066667
NorthernAfrica1.002000e+063.159170e+0597553.00000098.000000
NorthernAmerica9.833517e+061.803665e+07324460.00000035.500000
Polynesia2.842000e+037.740000e+02196.00000069.400000
South-easternAsia5.205436e+052.298626e+0576377.250000153.762500
SouthAmerica1.703150e+063.327079e+0542953.25000027.050000
SouthernAfrica6.918360e+051.093567e+0520494.66666741.166667
SouthernAsia9.843594e+054.851398e+05320367.800000197.200000
SouthernEurope2.874800e+041.154100e+042930.000000106.900000
WesternAfrica4.164142e+055.688491e+0430298.54545589.181818
WesternAsia2.060487e+051.389109e+0519296.700000243.040000
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" }, "metadata": {}, "execution_count": 85 } ], "source": [ "\n", "# Get the mean of each column for each region\n", "un_region.agg(['mean'])" ] }, { "cell_type": "code", "execution_count": 86, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " surface_area gdp \\\n", " mean median sum mean \n", "Region \n", "Caribbean 1.720500e+04 8868.0 86025 3.582980e+04 \n", "CentralAmerica 3.108195e+05 92104.5 2486556 1.712796e+05 \n", "CentralAsia 1.712745e+05 171274.5 342549 7.212500e+03 \n", "EasternAfrica 4.816671e+05 589626.5 5780005 1.883158e+04 \n", "EasternAsia 5.582058e+06 5582058.0 11164116 5.585108e+06 \n", "EasternEurope 3.186730e+05 318673.0 637346 4.854500e+04 \n", "Melanesia 2.054250e+04 20542.5 41085 9.060000e+02 \n", "Micronesia 5.490000e+02 549.0 549 -9.900000e+01 \n", "MiddleAfrica 1.054169e+06 475650.0 3162508 2.482600e+04 \n", "NorthernAfrica 1.002000e+06 1002000.0 1002000 3.159170e+05 \n", "NorthernAmerica 9.833517e+06 9833517.0 9833517 1.803665e+07 \n", "Polynesia 2.842000e+03 2842.0 2842 7.740000e+02 \n", "South-easternAsia 5.205436e+05 315483.5 4164349 2.298626e+05 \n", "SouthAmerica 1.703150e+06 927341.5 13625203 3.327079e+05 \n", "SouthernAfrica 6.918360e+05 824116.0 2075508 1.093567e+05 \n", "SouthernAsia 9.843594e+05 652864.0 4921797 4.851398e+05 \n", "SouthernEurope 2.874800e+04 28748.0 28748 1.154100e+04 \n", "WesternAfrica 4.164142e+05 238537.0 4580556 5.688491e+04 \n", "WesternAsia 2.060487e+05 78150.0 2060487 1.389109e+05 \n", "\n", " population \\\n", " median sum mean median sum \n", "Region \n", "Caribbean 8501.0 179149 5125.200000 3663.0 25626 \n", "CentralAmerica 38991.0 1370237 22164.750000 6298.0 177318 \n", "CentralAsia 7212.5 14425 7483.000000 7483.0 14966 \n", "EasternAfrica 13530.0 225979 25645.750000 17858.0 307749 \n", "EasternAsia 5585107.5 11170215 706296.500000 706296.5 1412593 \n", "EasternEurope 48545.0 97090 24137.000000 24137.0 48274 \n", "Melanesia 906.0 1812 443.500000 443.5 887 \n", "Micronesia -99.0 -99 164.000000 164.0 164 \n", "MiddleAfrica 28416.0 74478 36885.000000 24054.0 110655 \n", "NorthernAfrica 315917.0 315917 97553.000000 97553.0 97553 \n", "NorthernAmerica 18036648.0 18036648 324460.000000 324460.0 324460 \n", "Polynesia 774.0 774 196.000000 196.0 196 \n", "South-easternAsia 127921.0 1838901 76377.250000 61204.5 611018 \n", "SouthAmerica 145302.5 2661663 42953.250000 17340.0 343626 \n", "SouthernAfrica 11491.0 328070 20494.666667 2534.0 61484 \n", "SouthernAsia 20658.0 2425699 320367.800000 35530.0 1601839 \n", "SouthernEurope 11541.0 11541 2930.000000 2930.0 2930 \n", "WesternAfrica 11065.0 625734 30298.545455 15851.0 333284 \n", "WesternAsia 43833.0 1389109 19296.700000 9012.0 192967 \n", "\n", " population_density \n", " mean median sum \n", "Region \n", "Caribbean 323.140000 299.70 1615.7 \n", "CentralAmerica 104.262500 74.60 834.1 \n", "CentralAsia 47.600000 47.60 95.2 \n", "EasternAfrica 139.458333 54.35 1673.5 \n", "EasternAsia 76.050000 76.05 152.1 \n", "EasternEurope 99.800000 99.80 199.6 \n", "Melanesia 22.250000 22.25 44.5 \n", "Micronesia 304.100000 304.10 304.1 \n", "MiddleAfrica 34.066667 35.90 102.2 \n", "NorthernAfrica 98.000000 98.00 98.0 \n", "NorthernAmerica 35.500000 35.50 35.5 \n", "Polynesia 69.400000 69.40 69.4 \n", "South-easternAsia 153.762500 112.90 1230.1 \n", "SouthAmerica 27.050000 24.65 216.4 \n", "SouthernAfrica 41.166667 46.80 123.5 \n", "SouthernAsia 197.200000 204.40 986.0 \n", "SouthernEurope 106.900000 106.90 106.9 \n", "WesternAfrica 89.181818 82.30 981.0 \n", "WesternAsia 243.040000 107.10 2430.4 " ], "text/html": "
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surface_areagdppopulationpopulation_density
meanmediansummeanmediansummeanmediansummeanmediansum
Region
Caribbean1.720500e+048868.0860253.582980e+048501.01791495125.2000003663.025626323.140000299.701615.7
CentralAmerica3.108195e+0592104.524865561.712796e+0538991.0137023722164.7500006298.0177318104.26250074.60834.1
CentralAsia1.712745e+05171274.53425497.212500e+037212.5144257483.0000007483.01496647.60000047.6095.2
EasternAfrica4.816671e+05589626.557800051.883158e+0413530.022597925645.75000017858.0307749139.45833354.351673.5
EasternAsia5.582058e+065582058.0111641165.585108e+065585107.511170215706296.500000706296.5141259376.05000076.05152.1
EasternEurope3.186730e+05318673.06373464.854500e+0448545.09709024137.00000024137.04827499.80000099.80199.6
Melanesia2.054250e+0420542.5410859.060000e+02906.01812443.500000443.588722.25000022.2544.5
Micronesia5.490000e+02549.0549-9.900000e+01-99.0-99164.000000164.0164304.100000304.10304.1
MiddleAfrica1.054169e+06475650.031625082.482600e+0428416.07447836885.00000024054.011065534.06666735.90102.2
NorthernAfrica1.002000e+061002000.010020003.159170e+05315917.031591797553.00000097553.09755398.00000098.0098.0
NorthernAmerica9.833517e+069833517.098335171.803665e+0718036648.018036648324460.000000324460.032446035.50000035.5035.5
Polynesia2.842000e+032842.028427.740000e+02774.0774196.000000196.019669.40000069.4069.4
South-easternAsia5.205436e+05315483.541643492.298626e+05127921.0183890176377.25000061204.5611018153.762500112.901230.1
SouthAmerica1.703150e+06927341.5136252033.327079e+05145302.5266166342953.25000017340.034362627.05000024.65216.4
SouthernAfrica6.918360e+05824116.020755081.093567e+0511491.032807020494.6666672534.06148441.16666746.80123.5
SouthernAsia9.843594e+05652864.049217974.851398e+0520658.02425699320367.80000035530.01601839197.200000204.40986.0
SouthernEurope2.874800e+0428748.0287481.154100e+0411541.0115412930.0000002930.02930106.900000106.90106.9
WesternAfrica4.164142e+05238537.045805565.688491e+0411065.062573430298.54545515851.033328489.18181882.30981.0
WesternAsia2.060487e+0578150.020604871.389109e+0543833.0138910919296.7000009012.0192967243.040000107.102430.4
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" }, "metadata": {}, "execution_count": 86 } ], "source": [ "# Get mean, median, and sum\n", "un_region.agg(['mean', 'median', 'sum'])" ] }, { "source": [ "We can also pass in other functions or define our own. These are referred to as higher-order functions, i.e. functions that take in other functions as arguments. \n", "\n" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 101, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "6" ] }, "metadata": {}, "execution_count": 101 } ], "source": [ "# Example of higher-order function\n", "def add_two(val):\n", " return val + 2\n", "\n", "def do_calculation(val, func):\n", " return func(val)\n", "\n", "def subtract_nine(val):\n", " return val - 9\n", "\n", "do_calculation(12, add_two)\n", "do_calculation(15, subtract_nine)" ] }, { "cell_type": "code", "execution_count": 96, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "[1, 2, 6, 11]" ] }, "metadata": {}, "execution_count": 96 } ], "source": [ "# Example of filtering\n", "def is_nine(val):\n", " return val is not 9\n", "\n", "values = [1, 2, 6, 9, 9, 11]\n", "res = list(filter(is_nine, values))" ] }, { "source": [ "Let's use the statistics module from scipy to calculate some new values: https://docs.scipy.org/doc/scipy/reference/stats.html" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 100, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " surface_area gdp \\\n", " tsem tstd skew tsem \n", "Region \n", "Caribbean 9.321432e+03 2.084335e+04 0.685299 2.091220e+04 \n", "CentralAmerica 2.366682e+05 6.693987e+05 2.250712 1.387055e+05 \n", "CentralAsia 2.867450e+04 4.055187e+04 0.000000 6.405000e+02 \n", "EasternAfrica 9.050907e+04 3.135326e+05 -0.263652 5.318552e+03 \n", "EasternAsia 4.017942e+06 5.682228e+06 0.000000 5.573349e+06 \n", "EasternEurope 2.848270e+05 4.028062e+05 0.000000 4.207000e+04 \n", "Melanesia 8.353500e+03 1.181363e+04 0.000000 1.690000e+02 \n", "Micronesia NaN NaN 0.000000 NaN \n", "MiddleAfrica 6.464966e+05 1.119765e+06 0.695791 8.583308e+03 \n", "NorthernAfrica NaN NaN 0.000000 NaN \n", "NorthernAmerica NaN NaN 0.000000 NaN \n", "Polynesia NaN NaN 0.000000 NaN \n", "South-easternAsia 2.110175e+05 5.968477e+05 1.783458 1.038262e+05 \n", "SouthAmerica 9.847489e+05 2.785290e+06 2.162869 2.090794e+05 \n", "SouthernAfrica 3.500259e+05 6.062627e+05 -0.381757 1.026437e+05 \n", "SouthernAsia 5.934819e+05 1.327066e+06 1.284080 4.107010e+05 \n", "SouthernEurope NaN NaN 0.000000 NaN \n", "WesternAfrica 1.298416e+05 4.306358e+05 0.995896 4.390537e+04 \n", "WesternAsia 8.687679e+04 2.747285e+05 1.157696 7.048297e+04 \n", "\n", " population \\\n", " tstd skew tsem tstd \n", "Region \n", "Caribbean 4.676111e+04 0.607884 2435.292270 5445.479061 \n", "CentralAmerica 3.923185e+05 2.253862 15379.223415 43499.012664 \n", "CentralAsia 9.058038e+02 0.000000 1438.000000 2033.639103 \n", "EasternAfrica 1.842400e+04 1.434065 4595.949529 15920.836188 \n", "EasternAsia 7.881906e+06 0.000000 703220.500000 994503.968439 \n", "EasternEurope 5.949596e+04 0.000000 20086.000000 28405.893614 \n", "Melanesia 2.390021e+02 0.000000 167.500000 236.880772 \n", "Micronesia NaN 0.000000 NaN NaN \n", "MiddleAfrica 1.486673e+04 -0.417756 22879.972909 39629.275555 \n", "NorthernAfrica NaN 0.000000 NaN NaN \n", "NorthernAmerica NaN 0.000000 NaN NaN \n", "Polynesia NaN 0.000000 NaN NaN \n", "South-easternAsia 2.936648e+05 1.333305 30201.355172 85422.332172 \n", "SouthAmerica 5.913659e+05 2.125620 24368.804859 68925.388662 \n", "SouthernAfrica 1.777841e+05 0.704844 18111.375103 31369.821873 \n", "SouthernAsia 9.183553e+05 1.449172 257017.757855 574709.177989 \n", "SouthernEurope NaN 0.000000 NaN NaN \n", "WesternAfrica 1.456176e+05 2.814527 16239.477886 53860.254939 \n", "WesternAsia 2.228867e+05 1.993615 7749.004611 24504.504171 \n", "\n", " population_density \n", " skew tsem tstd skew \n", "Region \n", "Caribbean 0.215651 36.100853 80.723962 0.030881 \n", "CentralAmerica 2.214198 32.501153 91.927144 1.489869 \n", "CentralAsia 0.000000 16.100000 22.768838 0.000000 \n", "EasternAfrica 0.939269 47.199301 163.503175 1.306365 \n", "EasternAsia 0.000000 74.050000 104.722514 0.000000 \n", "EasternEurope 0.000000 23.500000 33.234019 0.000000 \n", "Melanesia 0.000000 0.450000 0.636396 0.000000 \n", "Micronesia 0.000000 NaN NaN 0.000000 \n", "MiddleAfrica 0.532459 10.288883 17.820868 -0.186994 \n", "NorthernAfrica 0.000000 NaN NaN 0.000000 \n", "NorthernAmerica 0.000000 NaN NaN 0.000000 \n", "Polynesia 0.000000 NaN NaN 0.000000 \n", "South-easternAsia 1.382405 40.638603 114.943327 0.866714 \n", "SouthAmerica 2.057515 7.109526 20.108776 0.932068 \n", "SouthernAfrica 0.707034 20.545586 35.585999 -0.283531 \n", "SouthernAsia 1.436007 77.116950 172.438743 0.439830 \n", "SouthernEurope 0.000000 NaN NaN 0.000000 \n", "WesternAfrica 2.732611 17.592038 58.346188 0.460914 \n", "WesternAsia 1.776751 84.348314 266.732788 1.290031 " ], "text/html": "
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surface_areagdppopulationpopulation_density
tsemtstdskewtsemtstdskewtsemtstdskewtsemtstdskew
Region
Caribbean9.321432e+032.084335e+040.6852992.091220e+044.676111e+040.6078842435.2922705445.4790610.21565136.10085380.7239620.030881
CentralAmerica2.366682e+056.693987e+052.2507121.387055e+053.923185e+052.25386215379.22341543499.0126642.21419832.50115391.9271441.489869
CentralAsia2.867450e+044.055187e+040.0000006.405000e+029.058038e+020.0000001438.0000002033.6391030.00000016.10000022.7688380.000000
EasternAfrica9.050907e+043.135326e+05-0.2636525.318552e+031.842400e+041.4340654595.94952915920.8361880.93926947.199301163.5031751.306365
EasternAsia4.017942e+065.682228e+060.0000005.573349e+067.881906e+060.000000703220.500000994503.9684390.00000074.050000104.7225140.000000
EasternEurope2.848270e+054.028062e+050.0000004.207000e+045.949596e+040.00000020086.00000028405.8936140.00000023.50000033.2340190.000000
Melanesia8.353500e+031.181363e+040.0000001.690000e+022.390021e+020.000000167.500000236.8807720.0000000.4500000.6363960.000000
MicronesiaNaNNaN0.000000NaNNaN0.000000NaNNaN0.000000NaNNaN0.000000
MiddleAfrica6.464966e+051.119765e+060.6957918.583308e+031.486673e+04-0.41775622879.97290939629.2755550.53245910.28888317.820868-0.186994
NorthernAfricaNaNNaN0.000000NaNNaN0.000000NaNNaN0.000000NaNNaN0.000000
NorthernAmericaNaNNaN0.000000NaNNaN0.000000NaNNaN0.000000NaNNaN0.000000
PolynesiaNaNNaN0.000000NaNNaN0.000000NaNNaN0.000000NaNNaN0.000000
South-easternAsia2.110175e+055.968477e+051.7834581.038262e+052.936648e+051.33330530201.35517285422.3321721.38240540.638603114.9433270.866714
SouthAmerica9.847489e+052.785290e+062.1628692.090794e+055.913659e+052.12562024368.80485968925.3886622.0575157.10952620.1087760.932068
SouthernAfrica3.500259e+056.062627e+05-0.3817571.026437e+051.777841e+050.70484418111.37510331369.8218730.70703420.54558635.585999-0.283531
SouthernAsia5.934819e+051.327066e+061.2840804.107010e+059.183553e+051.449172257017.757855574709.1779891.43600777.116950172.4387430.439830
SouthernEuropeNaNNaN0.000000NaNNaN0.000000NaNNaN0.000000NaNNaN0.000000
WesternAfrica1.298416e+054.306358e+050.9958964.390537e+041.456176e+052.81452716239.47788653860.2549392.73261117.59203858.3461880.460914
WesternAsia8.687679e+042.747285e+051.1576967.048297e+042.228867e+051.9936157749.00461124504.5041711.77675184.348314266.7327881.290031
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" }, "metadata": {}, "execution_count": 100 } ], "source": [ "# stats.tsem(), stats.tstd(), stats.skew()\n", "un_region.agg([stats.tsem, stats.tstd, stats.skew])" ] }, { "cell_type": "code", "execution_count": 102, "metadata": {}, "outputs": [], "source": [ "# Use a lambda function\n", "# https://www.geeksforgeeks.org/python-lambda-anonymous-functions-filter-map-reduce/\n", "\n", "get_max = lambda x : x.max()\n", "def get_max_alt(x):\n", " return x.max()" ] }, { "cell_type": "code", "execution_count": 107, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country surface_area \\\n", " get_max_alt get_max_alt \n", "Region \n", "Caribbean Virgin Islands 48671 \n", "CentralAmerica Panama 1964375 \n", "CentralAsia Tajikistan 199949 \n", "EasternAfrica Zimbabwe 947303 \n", "EasternAsia Mongolia 9600000 \n", "EasternEurope Ukraine 603500 \n", "Melanesia Vanuatu 28896 \n", "Micronesia Guam 549 \n", "MiddleAfrica The Democratic Republic of the Congo 2344858 \n", "NorthernAfrica Egypt 1002000 \n", "NorthernAmerica United States 9833517 \n", "Polynesia Samoa 2842 \n", "South-easternAsia Vietnam 1910931 \n", "SouthAmerica Suriname 8515767 \n", "SouthernAfrica South Africa 1221037 \n", "SouthernAsia Pakistan 3287263 \n", "SouthernEurope Albania 28748 \n", "WesternAfrica Togo 1240192 \n", "WesternAsia Yemen 783562 \n", "\n", " gdp population population_density \n", " get_max_alt get_max_alt get_max_alt \n", "Region \n", "Caribbean 102906 10981 413.0 \n", "CentralAmerica 1140724 129163 307.8 \n", "CentralAsia 7853 8921 63.7 \n", "EasternAfrica 63399 57310 494.9 \n", "EasternAsia 11158457 1409517 150.1 \n", "EasternEurope 90615 44223 123.3 \n", "Melanesia 1075 611 22.7 \n", "Micronesia -99 164 304.1 \n", "MiddleAfrica 37569 81340 50.9 \n", "NorthernAfrica 315917 97553 98.0 \n", "NorthernAmerica 18036648 324460 35.5 \n", "Polynesia 774 196 69.4 \n", "South-easternAsia 861934 263991 351.9 \n", "SouthAmerica 1772591 209288 66.9 \n", "SouthernAfrica 314571 56717 73.6 \n", "SouthernAsia 2116239 1339180 450.4 \n", "SouthernEurope 11541 2930 106.9 \n", "WesternAfrica 494583 190886 209.6 \n", "WesternAsia 717888 80745 817.4 " ], "text/html": "
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countrysurface_areagdppopulationpopulation_density
get_max_altget_max_altget_max_altget_max_altget_max_alt
Region
CaribbeanVirgin Islands4867110290610981413.0
CentralAmericaPanama19643751140724129163307.8
CentralAsiaTajikistan1999497853892163.7
EasternAfricaZimbabwe9473036339957310494.9
EasternAsiaMongolia9600000111584571409517150.1
EasternEuropeUkraine6035009061544223123.3
MelanesiaVanuatu28896107561122.7
MicronesiaGuam549-99164304.1
MiddleAfricaThe Democratic Republic of the Congo2344858375698134050.9
NorthernAfricaEgypt10020003159179755398.0
NorthernAmericaUnited States98335171803664832446035.5
PolynesiaSamoa284277419669.4
South-easternAsiaVietnam1910931861934263991351.9
SouthAmericaSuriname8515767177259120928866.9
SouthernAfricaSouth Africa12210373145715671773.6
SouthernAsiaPakistan328726321162391339180450.4
SouthernEuropeAlbania28748115412930106.9
WesternAfricaTogo1240192494583190886209.6
WesternAsiaYemen78356271788880745817.4
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" }, "metadata": {}, "execution_count": 107 } ], "source": [ "# Get the max of each column in each region\n", "un_region.agg([get_max_alt])" ] }, { "cell_type": "code", "execution_count": 127, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " get_max_alt\n", "Region \n", "Caribbean 48671\n", "CentralAmerica 1964375\n", "CentralAsia 199949\n", "EasternAfrica 947303\n", "EasternAsia 9600000\n", "EasternEurope 603500\n", "Melanesia 28896\n", "Micronesia 549\n", "MiddleAfrica 2344858\n", "NorthernAfrica 1002000\n", "NorthernAmerica 9833517\n", "Polynesia 2842\n", "South-easternAsia 1910931\n", "SouthAmerica 8515767\n", "SouthernAfrica 1221037\n", "SouthernAsia 3287263\n", "SouthernEurope 28748\n", "WesternAfrica 1240192\n", "WesternAsia 783562" ], "text/html": "
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get_max_alt
Region
Caribbean48671
CentralAmerica1964375
CentralAsia199949
EasternAfrica947303
EasternAsia9600000
EasternEurope603500
Melanesia28896
Micronesia549
MiddleAfrica2344858
NorthernAfrica1002000
NorthernAmerica9833517
Polynesia2842
South-easternAsia1910931
SouthAmerica8515767
SouthernAfrica1221037
SouthernAsia3287263
SouthernEurope28748
WesternAfrica1240192
WesternAsia783562
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" }, "metadata": {}, "execution_count": 127 } ], "source": [ "# Select a single column\n", "un_region['surface_area'].agg([get_max_alt])" ] }, { "cell_type": "code", "execution_count": 159, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " Region surface_area population\n", " sum mean mean\n", "0 Caribbean 86025 1.720500e+04 5125.200000\n", "1 CentralAmerica 2486556 3.108195e+05 22164.750000\n", "2 CentralAsia 342549 1.712745e+05 7483.000000\n", "3 EasternAfrica 5780005 4.816671e+05 25645.750000\n", "4 EasternAsia 11164116 5.582058e+06 706296.500000\n", "5 EasternEurope 637346 3.186730e+05 24137.000000\n", "6 Melanesia 41085 2.054250e+04 443.500000\n", "7 Micronesia 549 5.490000e+02 164.000000\n", "8 MiddleAfrica 3162508 1.054169e+06 36885.000000\n", "9 NorthernAfrica 1002000 1.002000e+06 97553.000000\n", "10 NorthernAmerica 9833517 9.833517e+06 324460.000000\n", "11 Polynesia 2842 2.842000e+03 196.000000\n", "12 South-easternAsia 4164349 5.205436e+05 76377.250000\n", "13 SouthAmerica 13625203 1.703150e+06 42953.250000\n", "14 SouthernAfrica 2075508 6.918360e+05 20494.666667\n", "15 SouthernAsia 4921797 9.843594e+05 320367.800000\n", "16 SouthernEurope 28748 2.874800e+04 2930.000000\n", "17 WesternAfrica 4580556 4.164142e+05 30298.545455\n", "18 WesternAsia 2060487 2.060487e+05 19296.700000" ], "text/html": "
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Regionsurface_areapopulation
summeanmean
0Caribbean860251.720500e+045125.200000
1CentralAmerica24865563.108195e+0522164.750000
2CentralAsia3425491.712745e+057483.000000
3EasternAfrica57800054.816671e+0525645.750000
4EasternAsia111641165.582058e+06706296.500000
5EasternEurope6373463.186730e+0524137.000000
6Melanesia410852.054250e+04443.500000
7Micronesia5495.490000e+02164.000000
8MiddleAfrica31625081.054169e+0636885.000000
9NorthernAfrica10020001.002000e+0697553.000000
10NorthernAmerica98335179.833517e+06324460.000000
11Polynesia28422.842000e+03196.000000
12South-easternAsia41643495.205436e+0576377.250000
13SouthAmerica136252031.703150e+0642953.250000
14SouthernAfrica20755086.918360e+0520494.666667
15SouthernAsia49217979.843594e+05320367.800000
16SouthernEurope287482.874800e+042930.000000
17WesternAfrica45805564.164142e+0530298.545455
18WesternAsia20604872.060487e+0519296.700000
\n
" }, "metadata": {}, "execution_count": 159 } ], "source": [ "# Get sum and mean of surface area, mean of population for each region\n", "un_region.agg({'surface_area':['sum', 'mean'], 'population': 'mean'})" ] }, { "cell_type": "code", "execution_count": 160, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " Region sum_surface_area mean_population\n", "0 Caribbean 86025 5125.200000\n", "1 CentralAmerica 2486556 22164.750000\n", "2 CentralAsia 342549 7483.000000\n", "3 EasternAfrica 5780005 25645.750000\n", "4 EasternAsia 11164116 706296.500000\n", "5 EasternEurope 637346 24137.000000\n", "6 Melanesia 41085 443.500000\n", "7 Micronesia 549 164.000000\n", "8 MiddleAfrica 3162508 36885.000000\n", "9 NorthernAfrica 1002000 97553.000000\n", "10 NorthernAmerica 9833517 324460.000000\n", "11 Polynesia 2842 196.000000\n", "12 South-easternAsia 4164349 76377.250000\n", "13 SouthAmerica 13625203 42953.250000\n", "14 SouthernAfrica 2075508 20494.666667\n", "15 SouthernAsia 4921797 320367.800000\n", "16 SouthernEurope 28748 2930.000000\n", "17 WesternAfrica 4580556 30298.545455\n", "18 WesternAsia 2060487 19296.700000" ], "text/html": "
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Regionsum_surface_areamean_population
0Caribbean860255125.200000
1CentralAmerica248655622164.750000
2CentralAsia3425497483.000000
3EasternAfrica578000525645.750000
4EasternAsia11164116706296.500000
5EasternEurope63734624137.000000
6Melanesia41085443.500000
7Micronesia549164.000000
8MiddleAfrica316250836885.000000
9NorthernAfrica100200097553.000000
10NorthernAmerica9833517324460.000000
11Polynesia2842196.000000
12South-easternAsia416434976377.250000
13SouthAmerica1362520342953.250000
14SouthernAfrica207550820494.666667
15SouthernAsia4921797320367.800000
16SouthernEurope287482930.000000
17WesternAfrica458055630298.545455
18WesternAsia206048719296.700000
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" }, "metadata": {}, "execution_count": 160 } ], "source": [ "# Define custom column names\n", "un_region.agg(sum_surface_area=('surface_area','sum'), mean_population=('population', 'mean'))" ] }, { "cell_type": "code", "execution_count": 167, "metadata": {}, "outputs": [], "source": [ "def inspect(df):\n", " print(df['population_density'] > 100)" ] }, { "cell_type": "code", "execution_count": 170, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country Region surface_area gdp \\\n", "1 Albania SouthernEurope 28748 11541 \n", "19 Dominican Republic Caribbean 48671 67103 \n", "25 Guam Micronesia 549 -99 \n", "27 Haiti Caribbean 27750 8501 \n", "59 Puerto Rico Caribbean 8868 102906 \n", "61 Saint Vincent and the Grenadines Caribbean 389 738 \n", "82 Virgin Islands Caribbean 347 -99 \n", "\n", " population population_density \n", "1 2930 106.9 \n", "19 10767 222.8 \n", "25 164 304.1 \n", "27 10981 398.4 \n", "59 3663 413.0 \n", "61 110 281.8 \n", "82 105 299.7 " ], "text/html": "
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countryRegionsurface_areagdppopulationpopulation_density
1AlbaniaSouthernEurope28748115412930106.9
19Dominican RepublicCaribbean486716710310767222.8
25GuamMicronesia549-99164304.1
27HaitiCaribbean27750850110981398.4
59Puerto RicoCaribbean88681029063663413.0
61Saint Vincent and the GrenadinesCaribbean389738110281.8
82Virgin IslandsCaribbean347-99105299.7
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" }, "metadata": {}, "execution_count": 170 } ], "source": [ "# .filter()\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.DataFrameGroupBy.filter.html\n", "# https://pandas.pydata.org/pandas-docs/stable/reference/groupby.html\n", "un_region.filter(lambda x: (x['population_density'] > 100).all())\n", "# un_region.filter(inspect)" ] }, { "cell_type": "code", "execution_count": 97, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ " country Region surface_area gdp population \\\n", "10 Burundi EasternAfrica 27830 2735 10864 \n", "21 Egypt NorthernAfrica 1002000 315917 97553 \n", "34 Kenya EasternAfrica 591958 63399 49700 \n", "40 Madagascar EasternAfrica 587295 9739 25571 \n", "41 Malawi EasternAfrica 118484 6420 18622 \n", "47 Mozambique EasternAfrica 799380 14806 29669 \n", "60 Rwanda EasternAfrica 26338 8096 12208 \n", "66 Somalia EasternAfrica 637657 1559 14742 \n", "68 South Sudan EasternAfrica 658841 13167 12576 \n", "71 Tanzania EasternAfrica 947303 45628 57310 \n", "77 Uganda EasternAfrica 241550 25282 42863 \n", "79 United States NorthernAmerica 9833517 18036648 324460 \n", "84 Zambia EasternAfrica 752612 21255 17094 \n", "85 Zimbabwe EasternAfrica 390757 13893 16530 \n", "\n", " population_density \n", "10 423.1 \n", "21 98.0 \n", "34 87.3 \n", "40 44.0 \n", "41 197.5 \n", "47 37.7 \n", "60 494.9 \n", "66 23.5 \n", "68 20.6 \n", "71 64.7 \n", "77 214.5 \n", "79 35.5 \n", "84 23.0 \n", "85 42.7 " ], "text/html": "
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countryRegionsurface_areagdppopulationpopulation_density
10BurundiEasternAfrica27830273510864423.1
21EgyptNorthernAfrica10020003159179755398.0
34KenyaEasternAfrica591958633994970087.3
40MadagascarEasternAfrica58729597392557144.0
41MalawiEasternAfrica118484642018622197.5
47MozambiqueEasternAfrica799380148062966937.7
60RwandaEasternAfrica26338809612208494.9
66SomaliaEasternAfrica63765715591474223.5
68South SudanEasternAfrica658841131671257620.6
71TanzaniaEasternAfrica947303456285731064.7
77UgandaEasternAfrica2415502528242863214.5
79United StatesNorthernAmerica98335171803664832446035.5
84ZambiaEasternAfrica752612212551709423.0
85ZimbabweEasternAfrica390757138931653042.7
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" }, "metadata": {}, "execution_count": 97 } ], "source": [ "un_region.filter(lambda x: (x.population > 10000).all())" ] }, { "source": [ "### Exercise - Get standard deviation of gdp for each region using np.std for all regions with a population density over 100" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 196, "metadata": {}, "outputs": [ { "output_type": "execute_result", "data": { "text/plain": [ "gdp 27227.285714\n", "dtype: float64" ] }, "metadata": {}, "execution_count": 196 } ], "source": [ "un_region.filter(lambda x: (x.population_density > 100).all()).agg({'gdp': np.mean})" ] }, { "source": [ "### Exercise - Generate corr plot for the UN data" ], "cell_type": "markdown", "metadata": {} }, { "cell_type": "code", "execution_count": 200, "metadata": {}, "outputs": [ { "output_type": "display_data", "data": { "text/plain": "
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\n" }, "metadata": { "needs_background": "light" } } ], "source": [ "# Select columns\n", "cols = un_data.columns\n", "\n", "# Make subplot and figure\n", "fig, ax = plt.subplots(1, 1)\n", "\n", "# Generate correlation matrix\n", "corr_data = un_data.corr()\n", "\n", "# Generate matplotlib plot\n", "cax = ax.matshow(corr_data) \n", "\n", "# Add colorbar to figure\n", "fig.colorbar(cax)\n", "\n", "# Set tick labels\n", "ax.set_xticklabels(cols)\n", "ax.set_yticklabels(cols)\n", "\n", "fig.tight_layout()" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] } ] } ================================================ FILE: samtools/binder/environment.yml ================================================ channels: - conda-forge - bioconda - defaults dependencies: - bash_kernel - samtools ================================================ FILE: samtools/data/subset.bed ================================================ 1 1 100 2 1 100 3 1 100 ================================================ FILE: samtools/index.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "\n", "## Intro to SAM/BAM format\n", "SAM (Sequence Alignment/Map) format is one of the most common file formats produced by many different pieces of alignment software, both for long and short read sequence data. It is a tab delineated text file, with 11 mandatory fields (listed below), plus header lines denoted with an `@` at the start of the line.\n", "\n", "SAM files are human readable, but can be quite large. An alternate format is the Binary Alignment/Map (BAM) file, which is binary compressed and not human readable, but is more compact and easy to work with. Most pipelines will use BAM format over SAM.\n", "\n", "\n", "## SAMtools\n", "[SAMtools](http://www.htslib.org/doc/samtools.html) is a suite of programs that are extremely useful for processing mapped reads and for downstream analysis. It has a ton of functions (which you can check out on the manual page), but we will go through several of the most common uses.\n", "\n", "### General pipeline\n", "Once you've obtained your mapped reads in BAM/SAM format (from BWA, bowtie, minimap, etc.), there are several steps needed before starting downstream analysis.\n", "\n", "*Filter*: generally this means removing unmapped reads from your file, which we will discuss below.\n", "\n", "*Sort*: sort the mapped reads by contig/scaffold and by coordinates. This can be done using `samtools sort`:\n", "\n", "`samtools sort -o data/file.sorted.bam data/file.bam`" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "*Index*: creates a searchable index of your sorted BAM file, which is required for some programs." ] }, { "cell_type": "code", "execution_count": null, "metadata": { "collapsed": true }, "outputs": [], "source": [ "samtools index data/file.sorted.bam" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "One of the most useful tools for the first processing of mapped reads is `samtools view`, which allows you to view the contents of a BAM/SAM file in SAM format:" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "collapsed": true }, "outputs": [], "source": [ "samtools view data/file.sorted.bam | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "\n", "| **Column**| **Description** |\n", "|-----|---|\n", "| 1 | Read name |\n", "| 2 | Bitwise flag |\n", "| 3 | Reference name |\n", "| 4 | Leftmost mapping position |\n", "| 5 | MAPQ quality score |\n", "| 6 | CIGAR string |\n", "| 7 | Name of 2nd read in pair |\n", "| 8 | Position of 2nd read in pair |\n", "| 9 | Length of mapping segment |\n", "| 10 | Sequence of segment |\n", "| 11 | Phred33 quality score at each position |\n" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "\n", "### Viewing specific regions\n", "By default `samtools view` prints all alignments, but you can specify a specific chromosome or subregion to only print alignments in that window:\n", "\n" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools view data/file.sorted.bam 1 | head\n", "samtools view data/file.sorted.bam 1:100-200 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "If you have numerous regions of interest, you can format them as a BED file and pass that to `samtools view`. This can be slow with large BAM files, as it does not does not use the index.\n" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "cat data/subset.bed" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools view -h -o data/file.subset.sam -L data/subset.bed data/file.sorted.bam" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "The `-o` option specifies an output file, rather than printing to screen. The `-h` option is important to remember, as it adds a header to the output. This is important, as many programs require a header to parse BAM/SAM files!" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Converting between formats\n", "By default `samtools view` outputs in SAM format, so converting from BAM --> SAM is as easy as running `samtools view -h -o outfile.sam file.bam`.\n", "\n", "For SAM --> BAM, include the `-b` option:\n", "`samtools view -b -h -o outfile.bam file.sam`\n", "\n" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### SAM flags\n", "The second column in a BAM/SAM file is the *bitwise flag*. The flag value is an integer, which is the sum of a series of decimal values that give information about how a read is mapped\n", "\n", "| **Integer**| **Description** |\n", "|-----|---|\n", "| 1 | read is paired |\n", "| 2 | read mapped in proper pair |\n", "| 4 | read unmapped |\n", "| 8 | mate is unmapped |\n", "| 16 | read on reverse strand |\n", "| 32 | mate on reverse strand |\n", "| 64 | first read in pair |\n", "| 128 | second read in pair |\n", "| 256 | not primary alignment |\n", "| 512 | alignment fails quality checks |\n", "| 1024 | PCR or optical duplicate |\n", "| 2048 | supplementary alignment |\n", "\n", "So e.g., for a paired-end mapping data set, a flag = **99** (1+2+32+64) means the read is mapped along with its mate (1 and 2) and in the proper orientation (32 and 64)." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Filtering reads\n", "Probably the most important flag to remember is **4**, which means the read is **unmapped**. Unmapped reads are most often filtered out. You can filter reads containing a given flag using the `-f` (only take reads that match given flags) and `-F` (only take reads that do **NOT** match given flag) options in `samtools view`.\n", "\n", "So to remove unmapped reads, you would run:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools view -F 4 data/file.sorted.bam | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This removes any read that contains the 4 flag (e.g. 77, 141, etc.). You can filter on any other criteria using flags as well, e.g. only gets reads that map in proper pair:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools view -f 2 data/file.sorted.bam | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "(Note this uses `-f`, not `-F`!)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## Downstream analysis\n", "\n", "### Calculating coverage\n", "One of the most common things you will want to know about your mapped reads is their coverage and depth, as this can impact your confidence in the assembly, the validity of your SNP calls, etc. There are many approaches you can take to calculate depth, several of which you can do with SAMtools. \n", "\n", "`samtools coverage`: for each contig/scaffold in the BAM/SAM file, outputs several useful summary stats as a table:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools coverage data/file.sorted.bam" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Like with `samtools view`, can also specify coordinates:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools coverage data/file.sorted.bam -r 1" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "As a quick way to visualize coverage, you can use the `-m` option create a histogram of coverage over a contig:\n" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools coverage -m data/file.sorted.bam -r 1:1-1000" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "You might also want to look at per-base coverage rather than the average. For this you can use `samtools depth`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools depth -a data/file.sorted.bam -r 1:1-1000 | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This outputs a three column list, where the 1st column is the contig name, the 2nd is the position, and the 3rd is the depth over that base. This list is convenient for importing to programs like R, where you can plot e.g. a histogram showing the distribution of per-base depth, or distribution of depth over a contig." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Stats\n", "Another useful function built into SAMtools is `samtools stats`, which gives some quick summary statistics about your mapping reads. The amount of information it generates is somewhat overkill in most cases, so we will just look at the summary:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools stats data/file.sorted.bam | grep ^SN | cut -f 2-" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## Other tricks\n", "\n", "### BAM to FASTQ/A\n", "If you want to extract the sequence info from the reads you can use `samtools fastq` or `samtools fasta`:\n" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools fastq data/file.sorted.bam | head" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "You can also output the pairs to different files.\n", "\n", "### Merge BAM files\n", "You can combine multiple sorted BAM/SAM files, which can be useful if you have done multiple rounds of mapping:\n", "\n", "`samtools merge file.bam file2.bam ...`\n", "\n", "Unless otherwise specified, the headers will also be merged.\n" ] }, { "cell_type": "code", "execution_count": null, "metadata": { "collapsed": true }, "outputs": [], "source": [] } ], "metadata": { "kernelspec": { "display_name": "Bash", "language": "bash", "name": "bash" }, "language_info": { "codemirror_mode": "shell", "file_extension": ".sh", "mimetype": "text/x-sh", "name": "bash" } }, "nbformat": 4, "nbformat_minor": 2 } ================================================ FILE: samtools/introduction.md ================================================ ## Intro to SAM/BAM format SAM (Sequence Alignment/Map) format is one of the most common file formats produced by many different pieces of alignment software, both for long and short read sequence data. It is a tab delineated text file, with 11 mandatory fields (listed below), plus header lines denoted with an `@` at the start of the line. SAM files are human readable, but can be quite large. An alternate format is the Binary Alignment/Map (BAM) file, which is binary compressed and not human readable, but is more compact and easy to work with. Most pipelines will use BAM format over SAM. ## SAMtools [SAMtools](http://www.htslib.org/doc/samtools.html) is a suite of programs that are extremely useful for processing mapped reads and for downstream analysis. It has a ton of functions (which you can check out on the manual page), but we will go through several of the most common uses. ### General pipeline Once you've obtained your mapped reads in BAM/SAM format (from BWA, bowtie, minimap, etc.), there are several steps needed before starting downstream analysis. *Filter*: generally this means removing unmapped reads from your file, which we will discuss below. *Sort*: sort the mapped reads by contig/scaffold and by coordinates. This can be done using `samtools sort`: `samtools sort -o file.sorted.bam file.bam` *Index*: creates a searchable index of your sorted BAM file, which is required for some programs. `samtools index file.sorted.bam` ___ One of the most useful tools for the first processing of mapped reads is `samtools view`, which allows you to view the contents of a BAM/SAM file in SAM format: `samtools view data/file.sorted.bam | head` | **Column**| **Description** | |-----|---| | 1 | Read name | | 2 | Bitwise flag | | 3 | Reference name | | 4 | Leftmost mapping position | | 5 | MAPQ quality score | | 6 | CIGAR string | | 7 | Name of 2nd read in pair | | 8 | Position of 2nd read in pair | | 9 | Length of mapping segment | | 10 | Sequence of segment | | 11 | Phred33 quality score at each position | ### Viewing specific regions By default `samtools view` prints all alignments, but you can specify a specific chromosome or subregion to only print alignments in that window: `samtools view data/file.sorted.bam 1 | head` `samtools view data/file.sorted.bam 1:1-1000 | head` If you have numerous regions of interest, you can format them as a BED file and pass that to `samtools view`. This can be slow with large BAM files, as it does not does not use the index. `samtools view -h -o file.subset.bam -L subset.bed file.bam` The `-o` option specifies an output file, rather than printing to screen. The `-h` option is important to remember, as it adds a header to the output. This is important, as many programs require a header to parse BAM/SAM files! ### Converting between formats By default `samtools view` outputs in SAM format, so converting from BAM --> SAM is as easy as running `samtools view -h -o outfile.sam file.bam`. For SAM --> BAM, include the `-b` option: `samtools view -b -h -o outfile.bam file.sam` ### SAM flags The second column in a BAM/SAM file is the *bitwise flag*. The flag value is an integer, which is the sum of a series of decimal values that give information about how a read is mapped | **Integer**| **Description** | |-----|---| | 1 | read is paired | | 2 | read mapped in proper pair | | 4 | read unmapped | | 8 | mate is unmapped | | 16 | read on reverse strand | | 32 | mate on reverse strand | | 64 | first read in pair | | 128 | second read in pair | | 256 | not primary alignment | | 512 | alignment fails quality checks | | 1024 | PCR or optical duplicate | | 2048 | supplementary alignment | So e.g., for a paired-end mapping data set, a flag = **99** (1+2+32+64) means the read is mapped along with its mate (1 and 2) and in the proper orientation (32 and 64). ### Filtering reads Probably the most important flag to remember is **4**, which means the read is **unmapped**. Unmapped reads are most often filtered out. You can filter reads containing a given flag using the `-f` (only take reads that match given flags) and `-F` (only take reads that do **NOT** match given flag) options in `samtools view`. So to remove unmapped reads, you would run: `samtools view -F 4 -h file.sorted.bam | head` This removes any read that contains the 4 flag (e.g. 77, 141, etc.). You can filter on any other criteria using flags as well, e.g. only gets reads that map in proper pair: `samtools view -f 2 -h file.sorted.bam` (Note this uses `-f`, not `-F`!) ## Downstream analysis ### Calculating coverage One of the most common things you will want to know about your mapped reads is their coverage and depth, as this can impact your confidence in the assembly, the validity of your SNP calls, etc. There are many approaches you can take to calculate depth, several of which you can do with SAMtools. `samtools coverage`: for each contig/scaffold in the BAM/SAM file, outputs several useful summary stats as a table: `samtools coverage file.sorted.bam` Like with `samtools view`, can also specify coordinates: `samtools coverage file.sorted.bam -r 1` As a quick way to visualize coverage, you can use the `-m` option create a histogram of coverage over a contig: `samtools coverage -m ERR1013163.sorted.bam -r 1:1-1000` You might also want to look at per-base coverage rather than the average. For this you can use `samtools depth`: `samtools depth -a file.sorted.bam -r 1:1-1000 | head` This outputs a three column list, where the 1st column is the contig name, the 2nd is the position, and the 3rd is the depth over that base. This list is convenient for importing to programs like R, where you can plot e.g. a histogram showing the distribution of per-base depth, or distribution of depth over a contig. ### Stats/flagstats Another useful function built into SAMtools is `samtools stats`, which gives some quick summary statistics about your mapping reads. The amount of information it generates is somewhat overkill in most cases, so we will just look at the summary: `samtools stats ERR1013163.sorted.subset.bam | grep ^SN | cut -f 2-` ## Other tricks ### BAM to FASTQ/A If you want to extract the sequence info from the reads you can use `samtools fastq` or `samtools fasta`: `samtools fastq file.bam | head` You can also output the pairs to different files. ### Merge BAM files You can combine multiple sorted BAM/SAM files, which can be useful if you have done multiple rounds of mapping: `samtools merge file.bam file2.bam ...` Unless otherwise specified, the headers will also be merged. ================================================ FILE: singularity/images/singularity_container.drawio ================================================ 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================================================ FILE: singularity/part1.html ================================================ Title ================================================ FILE: singularity/part1.md ================================================ class: middle, center # Singularity ## Finding & Using Containers ### FAS Bioinformatics Workshop #### April 27, 2021 #### Your Host: Nathan Weeks --- # Roadmap ### Part 1 (this presentation) * What are (Singularity) containers? * Finding container images * Using Singularity on Cannon ### Part 2 (next week) * Building Singularity container images * Common pitfalls * Useful tips & tricks --- ### Prerequisites (to follow along with the exercises)... 1. Log into Cannon (either via [SSH](https://docs.rc.fas.harvard.edu/kb/terminal-access/), or a web browser using the [FASRC VDI portal](https://docs.rc.fas.harvard.edu/kb/virtual-desktop/) ([FASRC VPN](https://docs.rc.fas.harvard.edu/kb/vpn-setup/) connection required) 2. Launch an [interactive job](https://docs.rc.fas.harvard.edu/kb/running-jobs/#Interactive_jobs_and_salloc) on a compute node: ``` salloc -p test,shared -t 2:00:00 --mem=4g ``` *NOTE:* the `singularity` *command is not available on Cannon login nodes* --- # What is a *container*? A set of one or more processes (*running programs*) that share (at least) a different root file system ("/"; usually provided by a *container image*) than processes running outside of the container on the same host operating system kernel (*typically Linux*). --- # What is Singularity? * An open-source container platform for Linux - https://github.com/hpcng/singularity - [Old beta for macOS](https://sylabs.io/singularity-desktop-macos/) (don't bother) * Started in 2015 by Greg Kurtzer from Lawrence Berkeley National Laboratory; now commercially developed/supported by [Sylabs](https://sylabs.io/) * Provides software tooling for both building *container images* and creating *containers* in which commands are run * Used: - Primarily for HPC clusters - Cannon: [FASRC VDI portal](https://docs.rc.fas.harvard.edu/kb/virtual-desktop/) (Open OnDemand) to run JupyterLab, RStudio Server, and other interactive apps - Workflow management systems, such as [Snakemake](https://snakemake.readthedocs.io/en/stable/snakefiles/deployment.html#running-jobs-in-containers), [NextFlow](https://www.nextflow.io/docs/latest/singularity.html), and [Galaxy](https://docs.galaxyproject.org/en/master/admin/special_topics/mulled_containers.html) - [Open Science Grid](https://support.opensciencegrid.org/support/solutions/articles/12000024676-docker-and-singularity-containers) --- # Singularity Image * A Singularity *image* is a file containing a (compressed, read-only [SquashFS](https://en.wikipedia.org/wiki/SquashFS)) file system, usually containing: * A base (Linux) operating system (e.g., Ubuntu, CentOS, Alpine) * Target software (e.g., NCBI BLAST+) and all software dependencies * Typical file extension: *.sif* ([Singularity Image Format]()); older (pre Singularity 3.x) may use ".simg" * Since it's a *file*, can be copied to other hosts (e.g., `scp`), or archived/shared (e.g., alongside code and research data) ![singularity image](images/singularity_image.jpeg) --- # Singularity Container * A Singularity *container* runs user processes with a software environment reflecting the (read-only) file system within the Singularity image file, plus select (generally-writable) directories from the host that are *bind-mounted* onto this file system. * On Cannon, this includes **/n/** (network) file systems, like **/n/holyscratch01** and **/n/home* ** --- # Why use containers? --- # Why use containers? * Save time - (Re)creating a complex software environment on a different host can be difficult * Portability - A container image can run on another (Linux) host, or... - Easily shared with other users on the same system (e.g., on Cannon with other members of the same lab) * Reproducibility - Guarantees software environment (including exact versions) recorded & reproduced faithfully #### SEE ALSO * [Introduction to Singularity - Use Cases](https://sylabs.io/guides/3.7/user-guide/introduction.html#use-cases) --- ## Example: [Trinity](https://trinityrnaseq.github.io/) de novo transcriptome assembler * Difficult to install; many software dependencies - Bespoke environment modules for a couple older versions exist on Cannon (see `module-query trinityrnaseq`) * "Official" *container image* encapsulates Trinity & dependencies (see the [Dockerfile](https://github.com/trinityrnaseq/trinityrnaseq/blob/v2.12.0/Docker/Dockerfile) container image "recipe") - Can be used on Cannon
--- ## Running a command in a Singularity container [singularity exec](https://sylabs.io/guides/3.7/user-guide/quick_start.html#executing-commands) is the most common mechanism to run a command in singularity container, both for batch and interactive jobs: ``` singularity exec [...options...] singularity_image.sif command [...command arguments...] ``` e.g.: ``` $ image=/n/singularity_images/informatics/braker2/braker2_2.1.6.sif $ singularity exec ${image} which braker.pl /usr/local/bin/braker.pl $ singularity exec ${image} braker.pl --version braker.pl version 2.1.6 ``` *NOTE: It is good practice to use the `singularity exec --cleanenv` option; this will be discussed in more detail in part 2* --- ## Running a command in a Singularity container [singularity shell](https://sylabs.io/guides/3.7/user-guide/quick_start.html#shell) is used to get a "shell" in the container (almost like logging into a virtual machine) for interactive exploration and (short) interactive work: ``` $ singularity shell /n/singularity_images/informatics/maker/maker:3.01.03-repbase.sif Singularity> type maker maker is /usr/local/bin/maker Singularity> cat /etc/os-release PRETTY_NAME="Debian GNU/Linux 8 (jessie)" NAME="Debian GNU/Linux" VERSION_ID="8" VERSION="8 (jessie)" ID=debian HOME_URL="http://www.debian.org/" SUPPORT_URL="http://www.debian.org/support" BUG_REPORT_URL="https://bugs.debian.org/" ``` *The shell prompt changes to `Singularity>` to indicate the shell is in a container* --- ## Your Turn 1. Start a shell in an NCBI BLAST container using the `singularity shell` command: ``` singularity shell /n/singularity_images/informatics/ncbi-blast/ncbi-blast:2.10.0.sif ``` 2. Answer the following questions: 1. What is the base operating system of the container image? (*hint*: `cat /etc/os-release`) 2. Where is the location of the `blastn` executable? (*hint*: `type blastn` or `which blastn` or `command -v blastn`) --- ## Finding Container Images - Container Registries * Container images are typically hosted in a *container registry* - Hosting service for container *repositories*, analogous to git repositories * Many container registries have bad (or no) search interfaces, and may not be your first stop when looking for container images - Notable possible exception: NVIDIA GPU Accelerated Container Registry (NGC) for GPU-accelerated container images --- ## Singularity container registries 1. (*Update: now defunct?*) Singularity Hub (https://singularity-hub.org/) - The first Singularity container registry - Requires(d) exessive privileges to your GitHub account to be able to search / use 2. Sylabs Cloud Library (https://cloud.sylabs.io/library) - [singularity search](https://sylabs.io/guides/3.7/user-guide/cli/singularity_search.html) command can be used to search---but is not too useful (except for custom-built images) --- ### Container Registries - Docker / OCI (Open Container Image) Singularity can build SIF images from Docker / OCI images in container other registries. The most popular: * DockerHub - https://hub.docker.com/ - The original and most popular (public) container registry - [Image "pull" limits](https://www.docker.com/increase-rate-limits) - 100 anonymous image pulls per 6 hours per public IP address - 200 anonymous images pulls per (free) Docker account (see [singularity remote login](https://sylabs.io/guides/3.7/user-guide/cli/singularity_remote_login.html) to authenticate) * Quay - https://quay.io/search - *Caveat: looks like search will required RedHat SSO on July 1, 2021...* --- ## Other OCI Container Registries * GitHub Container Registry - https://docs.github.com/en/packages/guides/about-github-container-registry - Newish, but looks promising for continuous integration / automated builds of GitHub-hosted projects * GitLab Container Registry - https://docs.gitlab.com/ee/user/packages/container_registry/ - More mature than GitHub Container Registry; images hosted per-project / repository * NVIDIA GPU Accelerated Container Registry (NGC) - https://ngc.nvidia.com/ - singularity `--nv` option to use host GPU in container (see Singularity [GPU Support (NVIDIA CUDA & AMD ROCm](https://sylabs.io/guides/3.7/user-guide/gpu.html)) --- ## Other OCI Container Registries Mainly for paying customers: * Azure Container Registry - https://azure.microsoft.com/en-us/services/container-registry/ - Hosts some "native" Singularity images * Amazon Elastic Container Registry (ECR) - https://gallery.ecr.aws/ * Oracle Container Registry - https://container-registry.oracle.com/ --- # BioContainers * [Bioconda](https://bioconda.github.io/) is a bioinformatics-focused channel of software packages for the [conda](https://docs.rc.fas.harvard.edu/kb/python/) package manager - See earlier [conda tutorial](https://github.com/harvardinformatics/bioinformatics-coffee-hour/blob/master/taste-of-conda/index.ipynb) * [BioContainers](https://biocontainers.pro/) provides container images for (mostly) [Bioconda](https://bioconda.github.io/) packages (including dependencies) * Can search for BioContainers images: - [BioContainers registry](https://biocontainers.pro/registry) - [Bioconda Package Index](https://bioconda.github.io/conda-package_index.html) - Click on a package name > "container" link > tag > Fetch Tag (download icon) --- ## Generating a Singularity image from a Docker image Many container registries assume [Docker](https://docker.com), and suggest syntax like: ``` docker pull registry/user/repository:tag ``` To adapt to Singularity, replace with: ``` singularity pull docker://registry/user/repository:tag ``` ### Example ``` singularity pull --disable-cache docker://quay.io/biocontainers/samtools:1.12--h9aed4be_1 ``` * *The `--disable-cache` option prevents image layers from being cached in ${HOME}/.singularity/cache* --- ## Finding container images for bioinformatics Prefer "official" container images provided by the project; e.g. * Check docs, or for existence of Dockerfile in git repo * Examples: - [Trinity](https://github.com/trinityrnaseq/trinityrnaseq/wiki/Trinity-in-Docker#running-trinity-using-singularity) ``` curl -O https://data.broadinstitute.org/Trinity/TRINITY_SINGULARITY/\ trinityrnaseq.v2.12.0.simg ``` - FAS Informatics [Best Practices for De Novo Transcriptome Assembly with Trinity](https://informatics.fas.harvard.edu/best-practices-for-de-novo-transcriptome-assembly-with-trinity.html) illustrates optimized use on Cannon - [QIIME 2](https://docs.qiime2.org/2021.2/install/virtual/docker/) - `singularity pull --disable-cache docker://quay.io/qiime2/core:2021.2` - [MultiQC](https://github.com/ewels/MultiQC) - https://github.com/ewels/MultiQC - Links to: https://hub.docker.com/r/ewels/multiqc - `singularity pull --disable-cache docker://ewels/multiqc:1.10.1` * Existence of Dockerfile doesn't mean an image is avialable in a container registry (like Docker Hub) - e.g., [Augustus](https://github.com/Gaius-Augustus/Augustus) provides a Dockerfile, but needs to be built --- # CernVM-FS (CVMFS) * https://cernvm.cern.ch/fs/ * Singularity images of Biocontainers (maintained by the Galaxy Project) available at: ``` /cvmfs/singularity.galaxyproject.org/FIRST_LETTER/SECOND_LETTER/\ PACKAGE_NAME:VERSION--CONDA_BUILD ``` ex. ``` $ singularity exec --cleanenv \ /cvmfs/singularity.galaxyproject.org/b/l/blast:2.11.0--pl526he19e7b1_0 \ blastn -version WARNING: Skipping mount /var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container blastn: 2.11.0+ Package: blast 2.11.0, build Mar 12 2021 10:19:58 ``` * `/etc/resolv.conf` warning is a known issue: https://github.com/bioconda/bioconda-recipes/issues/11583 * https://docs.rc.fas.harvard.edu/kb/singularity-on-the-cluster/#BioContainers --- # CVMFS Caveats * Delay (up to a minute or so) when auto-mounting `/cvmfs/singularity.galaxyproject.org` on a given compute node * Further delay when fetching a container images ## Recommendations * Don't use for a [large number of jobs](https://docs.rc.fas.harvard.edu/kb/submitting-large-numbers-of-jobs/) * Copy frequently-used containers to [high-performance shared storage](https://docs.rc.fas.harvard.edu/kb/cluster-storage/#Networked_High-performance_Shared_Scratch_Storage) --- ## Exercise - Biocontainers & CVMFS 1. Choose a Biocontainers image, using your favorite interface: * [Bioconda package index](https://bioconda.github.io/conda-package_index.html) * [BioContainers Registry](https://biocontainers.pro/registry) `ls /cvmfs/singularity.galaxyproject.org/FIRST_LETTER/SECOND_LETTER/` 2. Find the container image in CVMFS, and execute a command in the container ``` singularity exec --cleanenv \ /cvmfs/singularity.galaxyproject.org/b/l/blast:2.11.0--pl526he19e7b1_0 \ blastn -version ``` 3. Copy & paste the `singularity exec` command into the chat --- # Miscellanea - Pipelines of Containers Processes in a Singularity container behave the same as processes on the host outside of a container w.r.t. I/O (including stdin/stdout/stderr). As such, they can be used with (unix) pipes: ``` singularity pull --disable-cache docker://quay.io/biocontainers/samtools:1.12--h9aed4be_1 singularity pull --disable-cache docker://quay.io/biocontainers/bwa-mem2:2.2.1--h9a82719_1 singularity exec --cleanenv bwa-mem2_2.2.1--h9a82719_1.sif bwa-mem2 index reference.fa.gz singularity exec --cleanenv bwa-mem2_2.2.1--h9a82719_1.sif \ bwa-mem2 mem reference.fa.gz left.fastq.gz right.fastq.gz | singularity exec --cleanenv samtools_1.12--h9aed4be_1.sif \ samtools sort --output-fmt bam > output.bam ``` ### SEE ALSO For a more detailed tutorial on Unix pipes, see [harvardinformatics/bioinformatics-coffee-hour/unix-pipes](https://github.com/harvardinformatics/bioinformatics-coffee-hour/blob/master/unix-pipes/index.ipynb) --- ## Links to other useful resources * Singularity User Guide - https://sylabs.io/guides/latest/user-guide/ * Software Carpentries - Introduction to Singularity (alpha) - https://carpentries-incubator.github.io/singularity-introduction * Creating and running software containers with Singularity (originally NIH) - https://singulari)ty-tutorial.github.io/ * Singularity Containers for Bioinformatics (Pawsey Supercomputing Centre) - https://pawseysc.github.io/containers-bioinformatics-workshop/ * BioContainers Registry: searching for bioinformatics tools, packages and containers - https://dx.doi.org/10.1021/acs.jproteome.0c00904 ================================================ FILE: snakemake/LICENSE ================================================ BSD 3-Clause License Copyright (c) 2019, C. Titus Brown All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the name of the copyright holder nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. ================================================ FILE: snakemake/README.md ================================================ # 2020-snakemake-Harvard-informatics-coffee-hour-demo Intro to workflows for efficient automated data analysis, using snakemake. See [the tutorial](tutorial.md) for more information! ================================================ FILE: snakemake/Snakefile ================================================ #my first Snakefile ================================================ FILE: snakemake/binder/README.md ================================================ # binder directory Configuration files for [mybinder](https://mybinder.org). ================================================ FILE: snakemake/binder/environment.yml ================================================ channels: - conda-forge - bioconda - defaults dependencies: - bash_kernel - snakemake>=5.2, <5.11 - fastqc - multiqc ================================================ FILE: snakemake/data/.gitignore ================================================ * !.gitignore ================================================ FILE: snakemake/index.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "## Intro to workflows for data analysis, using snakemake\n", "### Authors: Meghan Correa and Ming Tang\n", "### Bioinformatics Coffee Hour - May 26, 2020\n", "\n", "Modified from https://github.com/ctb/2019-snakemake-ucdavis\n", "\n", "No license; the below content is under CC0. (Do with it what you will, and I hope it's useful!)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Introduction and thoughts\n", "\n", "Hi! I'm Meghan Correa, I a member of the Software Operations team at FAS informatics. I support data analysis for the Bauer core among other things.\n", "\n", "This 25min teaser meant to show you how to get started using snakemake.\n", "\n", "Note, the only way you'll really learn to do all of this is by applying it to your own research and spending time on it :)." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Why use a workflow management tool?\n", "\n", "* Dependency management\n", "* Reentrancy - start back up where you left off\n", "* Reusable\n", "* Documented\n", "* Portable\n", "\n", "There are many to choose from:\n", "https://github.com/pditommaso/awesome-pipeline\n", "\n", "FAS Informatics choose to use snakemake for the post sequencing pipeline and other workflows mainly because it is written in python and is popular in the bioinformatics community." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Tasks we are going to do\n", "\n", "Inside the `data` folder, there are 4 fastq.gz files. We will use `Snakemake` do `fastqc` on each fastq files and compile multiple html files into a single one using `multiQC`." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## Software we're going to use\n", "\n", "We're going to be using [conda](https://conda.io/en/latest/) and [snakemake](https://snakemake.readthedocs.io/en/stable/), a well as packages from [bioconda](https://bioconda.github.io). If you wanted to run all of this on your own computer, you'll need to follow the bioconda install instructions.\n", "\n", "We'll be implementing a short read quality check pipeline, using [fastqc](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) and [multiqc](https://multiqc.info/). No worries if you don't know what any of this means, it's not super critical to the snakemake side of things :)\n", "\n", "You can see the full set of installed software requirements for python in `environment.yml` file and installs from apt repository in `apt.txt` in the binder folder of the repository.\n", "\n", "**Note we have installed all the required tools in the binder container**" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## Download some data\n", "\n", "Execute the below cell to fetch some data." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "curl -L https://github.com/ctb/2019-snakemake-ucdavis/raw/9db09bc0b6a3469f8a0d4996d4b2995bf36e5d27/data/0Hour_001_1.fq.gz > data/0Hour_001_1.fq.gz\n", "curl -L https://github.com/ctb/2019-snakemake-ucdavis/raw/9db09bc0b6a3469f8a0d4996d4b2995bf36e5d27/data/6Hour_001_1.fq.gz > data/6Hour_001_1.fq.gz\n", "curl -L https://github.com/ctb/2019-snakemake-ucdavis/raw/9db09bc0b6a3469f8a0d4996d4b2995bf36e5d27/data/0Hour_001_2.fq.gz > data/0Hour_001_2.fq.gz\n", "curl -L https://github.com/ctb/2019-snakemake-ucdavis/raw/9db09bc0b6a3469f8a0d4996d4b2995bf36e5d27/data/6Hour_001_2.fq.gz > data/6Hour_001_2.fq.gz" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## Running snakemake!\n", "\n", "### Getting started - your first Snakefile\n", "\n", "Open the `Snakemake` file with a text editor and paste the below into it:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule fastqc_a_file:\n", " shell:\n", " \"fastqc data/0Hour_001_1.fq.gz\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "(I suggest copy/pasting this into the Snakefile.)\n", "\n", "remember to click `File` --> `Save File`." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "and you should see:\n", "```\n", "Building DAG of jobs...\n", "Using shell: /bin/bash\n", "Provided cores: 1\n", "Rules claiming more threads will be scaled down.\n", "...\n", "Approx 95% complete for 0Hour_001_1.fq.gz\n", "Analysis complete for 0Hour_001_1.fq.gz\n", "[Wed Feb 27 13:09:51 2019]\n", "Finished job 0.\n", "1 of 1 steps (100%) done\n", "Complete log: /home/jovyan/.snakemake/log/2019-02-27T130941.260352.snakemake.log\n", "```" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "and there will be two new files,\n", "\n", "Points to make:\n", "* the snakemake configuration file is by default called `Snakefile`" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Updating the Snakefile to track inputs and outputs\n", "\n", "At the moment this is basically just a shell script with extra syntax... what's the point?\n", "\n", "Well, shell scripts will rerun the command every time you run the file, even if there's no reason to do so because the file hasn't changed but with snakemake, you can annotate the rule with input and output files!\n", "\n", "**Digression:** This is particularly important for large or long workflows, where you're dealing with 10s to 100s of files that may take hours to days to process! It can be hard to figure out which files to rerun, but (spoiler alert) snakemake can really help you do this!\n", "\n", "Change your snakefile to look like this:\n" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule fastqc_a_file:\n", " input:\n", " \"data/0Hour_001_1.fq.gz\"\n", " output:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/0Hour_001_1_fastqc.zip\"\n", " shell:\n", " \"fastqc data/0Hour_001_1.fq.gz\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "here, we've annotated the rule with the required\n", "**input** file, as well as the expected **output** files.\n", "\n", "Question: how do we know what the output files are?\n", "\n", "Now run:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "and you should see:\n", "```\n", "Building DAG of jobs...\n", "Nothing to be done.\n", "Complete log: /home/jovyan/.snakemake/log/2019-02-27T132031.813143.snakemake.log\n", "```" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "What happened??\n", "\n", "snakemake looked at the file, saw that the output files existed, and figured out that it didn't need to do anything!\n", "\n", "### Forcibly re-running things\n", "\n", "You can tell snakemake to run all the rules no matter what with `-F`:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake -F" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "You can also remove an output file and it will automatically re-run:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rm data/*.html\n", "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "note that you don't need to remove *all* the output files to rerun a command - just remove *one* of them.\n", "\n", "You can *also* update the timestamp on an *input* file, and snakemake will figure out that the output file is older than the input file, and rerun things." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "touch data/*.fq.gz\n", "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Another usefule snakemake option is the --dryrun option, this will show you what snakemake would do but won't actually run any of the rules. I highly recommend running this to check that was will be run is what you expect." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake --dryrun" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Multiple rules\n", "\n", "Let's add a rule to run fastqc on a second file:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule fastqc_a_file:\n", " input:\n", " \"data/0Hour_001_1.fq.gz\"\n", " output:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/0Hour_001_1_fastqc.zip\"\n", " shell:\n", " \"fastqc data/0Hour_001_1.fq.gz\"\n", "\n", "rule fastqc_a_file2:\n", " input:\n", " \"data/6Hour_001_1.fq.gz\"\n", " output:\n", " \"data/6Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.zip\"\n", " shell:\n", " \"fastqc data/6Hour_001_1.fq.gz\"" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Now, if you run this, the Right Thing won't happen: snakemake will do nothing. Why?\n", "\n", "Well, snakemake only runs the *first* rule in a Snakefile, by default. You can give a rule name on the command line, if you like, **or** you can tell snakemake what output file(s) you want. " ] }, { "cell_type": "raw", "metadata": {}, "source": [ "snakemake data/0Hour_001_1_fastqc.html data/6Hour_001_1_fastqc.html" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "But the conventional way to do this in snakemake is to add an \"all\" rule as the first rule, let's try it. \n", "\n", "\n", "### A first refactoring: adding a better default rule\n", "\n", "Let's start refactoring (cleaning up) this Snakefile.\n", "\n", "First, let's add a rule at the top:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule all:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"data/0Hour_001_1.fq.gz\"\n", " output:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/0Hour_001_1_fastqc.zip\"\n", " shell:\n", " \"fastqc data/0Hour_001_1.fq.gz\"\n", "\n", "rule fastqc_a_file2:\n", " input:\n", " \"data/6Hour_001_1.fq.gz\"\n", " output:\n", " \"data/6Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.zip\"\n", " shell:\n", " \"fastqc data/6Hour_001_1.fq.gz\"" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This is a blank rule that gathers together all of the various files you want produced, and says \"hey, snakemake, I depend on all of these files for my input - make them for me!\"\n", "\n", "Let's run snakemake again and now you should see the second fastqc command run, with the appropriate output files!\n", "\n", "Note that snakemake only runs the second rule, because it looks at the output files and sees that the first file you wanted, `0Hour_001_1_fastqc.html` already exists!\n", "\n", "This is a blank rule that gathers together all of the various files you want produced, and says \"hey, snakemake, I depend on all of these files for my input - make them for me!\"\n", "\n", "### A second refactoring: doing a bit of templating\n", "\n", "There's a lot of repetition in each of these rules. Let's collapse it down a little bit by replacing the filename in the fastqc command with a magic variable, `{input}`." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule all:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"data/0Hour_001_1.fq.gz\"\n", " output:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/0Hour_001_1_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"\n", "\n", "rule fastqc_a_file2:\n", " input:\n", " \"data/6Hour_001_1.fq.gz\"\n", " output:\n", " \"data/6Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This all works as before, but now the rule is a bit more generic and will work with any input file. Sort of.\n", "\n", "### Refactoring 3: templating output files, too\n", "\n", "The output filenames ALSO depend on the input file names in some way - specifically, fastqc replace part of the filename with `_fastqc.html` and `_fastqc.zip` to make its two output files.\n", "\n", "Let's rewrite the rule using some snakemake pattern matching:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule all:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"\n", "\n", "rule fastqc_a_file2:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "What we've done here is tell snakemake that anytime we say we *want* a file that ends with `_fastqc.html`, it should look for a file that ends in `.fq.gz` and then run `fastqc` on it." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "If you look at the rule above, they are now identical rules! \n", "\n", "Let's remove one, to get a trimmer, leaner, and above all *functional* snakefile:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule all:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note that the variable name in input and output does not have to be \"filename\", it can be anything as long as you're consistant. So let's run snakemake again." ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rm data/*.html\n", "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Building out the workflow\n", "\n", "So, we've gotten fastqc sorted out. What's next?\n", "\n", "Let's add in a new rule - multiqc, to summarize our fastqc results.\n", "\n", "multiqc takes a directory name under which there are one or more fastqc reports, and then summarizes them.\n", "\n", "Running it on the command line," ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "multiqc data" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "you can see that it creates two outputs, `multiqc_report.html` and the directory `multiqc_data/` which contains a bunch of files. Let's create a snakemake rule for this; add:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule all:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"\n", "\n", "rule run_multiqc:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\",\n", " output:\n", " \"multiqc_report.html\",\n", " directory(\"multiqc_data\")\n", " shell:\n", " \"multiqc data/\"" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This ...doesn't really do what we want, for a few reasons.\n", "\n", "First of all, the output of run_multiqc is not specified in the all rule so snakemake doesn't look for a rule to create this. \n", "\n", "Second of all, `multiqc_report.html` already exists, so snakemake won't run the rule. \n", "\n", "Let's fix the first two things first:\n", "\n", "* add `multiqc_report.html` to the inputs for the first all.\n", "* then remove `multiqc_report.html` and re-run snakemake.\n", "\n", "Your snakefile should look like:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rule all:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\",\n", " \"multiqc_report.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"\n", "\n", "rule run_multiqc:\n", " input:\n", " \"data/0Hour_001_1_fastqc.html\",\n", " \"data/6Hour_001_1_fastqc.html\",\n", " output:\n", " \"multiqc_report.html\",\n", " directory(\"multiqc_data\")\n", " shell:\n", " \"multiqc data/\"" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rm multiqc_report.html\n", "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Yay, that seems to work!\n", "\n", "Points to make:\n", "\n", "* other than the first rule, rules can be in any order\n", "* the rule name doesn't really matter, it's mostly for debugging. It just needs to be \"boring\" (text, underscores, etc. only)\n", "\n", "\n", "But providing input files explicitly to the multiqc rule is not great because those are the same files we have in the all rule and to add new files we'd now have to add them to two places. " ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "We can fix the first issue by using **variables**. \n", "\n", "To use variables, let's make a Python list at the very top, containing all of our expected output files from fastqc:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "fastqc_output = [\"data/0Hour_001_1_fastqc.html\", \"data/6Hour_001_1_fastqc.html\",\n", " \"data/0Hour_001_2_fastqc.html\", \"data/6Hour_001_2_fastqc.html\"]" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "and modify the `all` and `multiqc` rules to contain this list. The final snakefile looks like this:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "fastqc_output = [\"data/0Hour_001_1_fastqc.html\", \"data/6Hour_001_1_fastqc.html\",\n", " \"data/0Hour_001_2_fastqc.html\", \"data/6Hour_001_2_fastqc.html\"]\n", "\n", "rule all:\n", " input:\n", " fastqc_output,\n", " \"multiqc_report.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"\n", "\n", "rule run_multiqc:\n", " input:\n", " fastqc_output\n", " output:\n", " \"multiqc_report.html\",\n", " directory(\"multiqc_data\")\n", " shell:\n", " \"multiqc data/\"" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Refactoring this to make it slightly more concise --\n", "\n", "We've got one more redundancy in this file - the `fastqc_output` is listed in the `all` rule, but you don't need it there! Why?\n", "\n", "Well, `multiqc_report.html` is already in the all rule, and the multiqc rule depends on `fastqc_output`, so `fastqc_output` already needs to be created to satisfy the all rule, so... specifying it in the all rule is redundant! And you can remove it!\n", "\n", "(It's not a big deal and I usually leave it in. But I wanted to talk about dependencies!)\n", "\n", "The Snakefile now looks like this:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "fastqc_output = [\"data/0Hour_001_1_fastqc.html\", \"data/6Hour_001_1_fastqc.html\",\n", " \"data/0Hour_001_2_fastqc.html\", \"data/6Hour_001_2_fastqc.html\"]\n", "\n", "rule all:\n", " input:\n", " \"multiqc_report.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " shell:\n", " \"fastqc {input}\"\n", "\n", "rule run_multiqc:\n", " input:\n", " fastqc_output\n", " output:\n", " \"multiqc_report.html\",\n", " directory(\"multiqc_data\")\n", " shell:\n", " \"multiqc data/\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "and we can rerun it from scratch by doing:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "rm data/*.html multiqc_report.html\n", "snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Recap\n", "\n", "So, we've put all this work into making this snakefile with its input rules and its output rules... and there are a lot of advantages to our current approach already! Let's list a few of them --\n", "\n", "* we've completely automated our analysis!\n", "* we can easily add new data files into fastqc and multiqc!\n", "* we can rerun things easily, and (even better) by default only things that *need* to be run will be run.\n", "* the snakefile is actually pretty reusable - we could drop this into a new project, and, with little effort, run all of these things on new data!" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## More advanced snakemake" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Running things in parallel\n", "\n", "You can use `snakemake --cores 4` to run four jobs in parallel. If you are running on a cluster then --cores becomes the number of jobs that can be submitted at a time. \n", "\n", "### Specifying software required for a rule\n", "\n", "You can specify software on a per-rule basis! This is really helpful when you have incompatible software requirements for different rules, or want to run on a cluster, or just want to pin your snakemake workflow to a specific version.\n", "\n", "For example, if you create a file `env_fastqc.yml` with the following content," ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "channels:\n", " - bioconda\n", " - defaults\n", " - conda-forge\n", "dependencies:\n", " - fastqc==0.11.8" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "and then change the fastqc rule to look like this:" ] }, { "cell_type": "raw", "metadata": {}, "source": [ "rule fastqc_a_file:\n", " input:\n", " \"{filename}.fq.gz\"\n", " output:\n", " \"{filename}_fastqc.html\",\n", " \"{filename}_fastqc.zip\"\n", " conda:\n", " \"env_fastqc.yml\"\n", " shell:\n", " \"fastqc {input}\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "you can now run snakemake like so," ] }, { "cell_type": "raw", "metadata": {}, "source": [ "snakemake --use-conda" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "and for that rule, snakemake will install just that software, with the specified version.\n", "\n", "This aids in reproducibility, in addition to the practical advantages of isolating software installs from each other.\n", "\n", "(You can also do this with docker and singularity containers, too!)\n", "\n", "### Running on a cluster\n", "\n", "You can specify a cluster submit command:\n", "\n", "snakemake --cluster sbatch -j 32\n", "\n", "You can also specify per rule cluster config in a file (example below):\n", "\n", "snakemake --cluster sbatch --cluster-config cluster-config.json -j 32 \n" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "{\n", " \"default\":{\n", " \"time\":\"12:00:00\",\n", " \"nodes\":1,\n", " \"cores\":24,\n", " \"mem\":100,\n", " \"partition\":\"shared\",\n", " \"job-name\":\"{rule}\",\n", " \"output\":\"log/{rule}-%j.out\",\n", " \"error\":\"log/{rule}-%j.err\"\n", " },\n", " \"fastqc\":{\n", " \"time\":\"4-12:00:00\",\n", " \"mem\":32000\n", " },\n", " \"multiqc\":{\n", " \"mem\":64000\n", " }\n", "}" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Or you can use snakemake profiles and bundle cluster config files in a directory:\n", "\n", "snakemake --profile slurm -j 32\n", "\n", "https://snakemake.readthedocs.io/en/v5.18.1/executing/cli.html#profiles" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Adding in some Python...\n", "\n", "You can add in some Python to load in the input files, like so:" ] }, { "cell_type": "raw", "metadata": {}, "source": [ "import glob, sys\n", "fastqc_input = glob.glob('data/?Hour_00?_?.fq.gz')\n", "\n", "fastqc_output = []\n", "for filename in fastqc_input:\n", " new_filename = filename.split('.')[0] + '_fastqc.html'\n", " fastqc_output.append(new_filename)\n", "\n", "rule all:\n", " input:\n", " \"multiqc_report.html\"\n", "\n", "rule clean:\n", " shell:\n", " \"rm -f {fastqc_output} multiqc_report.html\"\n", "\n", "rule fastqc_a_file:\n", " input:\n", " \"{arglebarf}.fq.gz\"\n", " output:\n", " \"{arglebarf}_fastqc.html\",\n", " \"{arglebarf}_fastqc.zip\"\n", " conda:\n", " \"env_fastqc.yml\"\n", " shell:\n", " \"fastqc {input}\"\n", "\n", "\n", "rule run_multiqc:\n", " input:\n", " fastqc_output\n", " output:\n", " \"multiqc_report.html\",\n", " directory(\"multiqc_data\")\n", " shell:\n", " \"multiqc data/\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Go forth and workflow!" ] } ], "metadata": { "kernelspec": { "display_name": "Bash", "language": "bash", "name": "bash" }, "language_info": { "codemirror_mode": "shell", "file_extension": ".sh", "mimetype": "text/x-sh", "name": "bash" } }, "nbformat": 4, "nbformat_minor": 4 } ================================================ FILE: taste-of-conda/binder/environment.yml ================================================ dependencies: - bash_kernel ================================================ FILE: taste-of-conda/index.ipynb ================================================ {"metadata":{"kernelspec":{"display_name":"Bash","language":"bash","name":"bash"},"language_info":{"codemirror_mode":"shell","file_extension":".sh","mimetype":"text/x-sh","name":"bash"}},"nbformat_minor":4,"nbformat":4,"cells":[{"cell_type":"markdown","source":"# A Taste of Conda\n## Bioinformatics Coffee Hour - April 28, 2020\n#### Your Host: Nathan Weeks","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"## Problems\n#### How do I install scientific software on _INSERT LINUX SERVER OR CLUSTER NAME HERE_ as an unprivileged user?\n##### (and how do I get the same software on my workstation?)\n#### How do I create a reproducible software environment for data analysis?","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"### Possible solutions\n* [Environment modules](https://docs.rc.fas.harvard.edu/kb/modules-intro/)\n - (+) Maintained by FAS RC staff\n - (+) Easy to use\n - (-) New / updated software requests: submit ticket (wait...)\n - (-) Not reproducible outside of Cannon (mostly)\n* language-specific (e.g., [pip](https://pip.pypa.io/) (Python))\n* [Singularity containers](https://docs.rc.fas.harvard.edu/kb/singularity-on-the-cluster/) (future topic...)","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"### Conda is...\n> Package, dependency and environment management for any language--Python, R, Ruby, Lua, Scala, Java, JavaScript, C/ C++, FORTRAN, and more. (source: https://docs.conda.io)","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"### Where can I get conda... for my workstation?\n* [Anaconda Python](https://www.anaconda.com/distribution/)\n - 100s of bundled scientific packages\n - Anaconda Navigator (GUI for launching apps & installing packages)\n![Anaconda Navigator](https://docs.anaconda.com/_images/nav-defaults.png)","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"* [Miniconda](https://docs.conda.io/en/latest/miniconda.html)\n - Minimal python+conda environment\n - Can be installed in your home directory on a Linux cluster (not necessary on Cannon...)","metadata":{"slideshow":{"slide_type":"fragment"}}},{"cell_type":"markdown","source":"#### Cannon\nFind latest version with `module-query Anaconda3`, or searching the [FAS RC Application Portal](https://portal.rc.fas.harvard.edu/p3/build-reports/Anaconda3)\n```\n$ module load Anaconda3/2019.10\n```\n\n*For this lesson, we'll use the conda bundled with this Jupyter Lab Binder image*","metadata":{"slideshow":{"slide_type":"subslide"}}},{"cell_type":"markdown","source":"## Channel\n* Package set maintained by an organization\n* Main ones a computational biologist will use:\n","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"#### 1. [defaults](https://anaconda.org/anaconda/repo)\n - Maintained by Anaconda, Inc.","metadata":{"slideshow":{"slide_type":"fragment"}}},{"cell_type":"markdown","source":"#### 2. [conda-forge](https://conda-forge.org/)\n - Large community-led package collection\n - *Well-curated, up-to-date*","metadata":{"slideshow":{"slide_type":"fragment"}}},{"cell_type":"markdown","source":"#### 3. [bioconda](https://bioconda.github.io)\n - Specializes in bioinformatics software","metadata":{"slideshow":{"slide_type":"fragment"}}},{"cell_type":"markdown","source":"## Where to find Conda packages\n- [anaconda.org](https://anaconda.org/)\n- [Bioconda recipe index](https://bioconda.github.io/conda-recipe_index.html)","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"## An interactive walkthrough...","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"---","metadata":{"slideshow":{"slide_type":"slide"}}},{"cell_type":"markdown","source":"#### Getting help\nInvoke `conda` with the `-h` or `--help` option to display a list of subcommands.\n- Displays usage for subcommands (e.g., `conda list -h`)","metadata":{}},{"cell_type":"code","source":"conda --help","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"## Setting conda channels for bioinformatics\n\nbioconda packages may have dependencies on packages in the *conda-forge* and *defaults* channels.\nWe can specify channels to search as command-line arguments for conda operations, e.g.:\n\n`conda search -c conda-forge -c bioconda bwa`\n\nHowever, it is convenient to configure a default list of channels.\nPer [the bioconda documentation](https://bioconda.github.io/user/install.html#set-up-channels):","metadata":{}},{"cell_type":"code","source":"conda config --add channels defaults\nconda config --add channels bioconda\nconda config --add channels conda-forge","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"---\n*Pro tip:* Set `channel_priority` to `strict` to [speed up conda package searches](https://docs.conda.io/projects/conda/en/latest/user-guide/concepts/conda-performance.html#set-strict-channel-priority)","metadata":{}},{"cell_type":"code","source":"conda config --set channel_priority strict","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"*Note:* strict channel priority will be the [default in conda 5.0](https://docs.conda.io/projects/conda/en/latest/user-guide/tasks/manage-channels.html#strict-channel-priority)","metadata":{}},{"cell_type":"markdown","source":"We can verify the list of channels:","metadata":{}},{"cell_type":"code","source":"conda info","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"#### Searching for packages with conda","metadata":{}},{"cell_type":"markdown","source":"Use `conda search` to search for packages by name.\n\nE.g., to search for the package `bwa` (exact match)","metadata":{}},{"cell_type":"code","source":"conda search bwa","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"The `*` character can be used as a wildcard.\n\nE.g., to search for all packages *beginning* with the string \"bwa\":","metadata":{}},{"cell_type":"code","source":"conda search 'bwa*'","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"### Environments","metadata":{}},{"cell_type":"markdown","source":"Conda packages can be installed into separate *environments* (directories containing separate sets of conda packages).\n\nconda is installed into a \"base\" environment.","metadata":{}},{"cell_type":"code","source":"conda env list","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"#### Listing packages installed in an environment\nWe can see what packages are already installed in our current environment using `conda list`","metadata":{}},{"cell_type":"code","source":"conda list","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"*Never install packages into the base environment*. Always create a new environment.\n- base environment is read-only on Cannon","metadata":{}},{"cell_type":"markdown","source":"#### Creating a new environment","metadata":{}},{"cell_type":"code","source":"conda create -h","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"The `-n/--name ENVIRONMENT` option creates a \"named\" conda environment in your \"envs\" directory (by default `${HOME}/.conda/envs`).\n\nLet's create an environment called *bwa*:","metadata":{}},{"cell_type":"code","source":"conda create -y -n bwa","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"Verify the new environment was created:","metadata":{}},{"cell_type":"code","source":"conda env list","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"---","metadata":{}},{"cell_type":"markdown","source":"*Pro tip (Jupyter notebooks & conda via environment module)*: activate the _base_ environment before issuing any subsequent `conda activate` / `conda deactivate` commands ([gory details...](https://github.com/conda/conda/issues/7980))","metadata":{}},{"cell_type":"code","source":"source activate","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"*Note: currently slightly-incompatible with Jupyter notebooks; will cause subsequent commands to display error exit status `: 1`*","metadata":{}},{"cell_type":"markdown","source":"---","metadata":{}},{"cell_type":"markdown","source":"Before we activate the new *bwa* environment, let's check our PATH environment variable (list of directories your shell searches for commands)","metadata":{}},{"cell_type":"code","source":"echo ${PATH}","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"Activate the new \"bwa\" environment.\nSubsequent \nNotice that the `/srv/conda/envs/bwa/bin` directory was prepended to your PATH.\nIn addition, in an interactive shell, the shell prompt would be prefixed with `(bwa)`.","metadata":{}},{"cell_type":"code","source":"conda activate bwa\necho ${PATH}","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"code","source":"conda install -y bwa","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"In addition to named environments, we can also create an environment in an arbitrary directory using the `-p PATH` option. This can be useful for installing software in a shared directory that is accessible by your lab/group.\n\nSuppose we want to install samtools at `/srv/shiny-server/sample-apps/samtools` (*Note: this directory is just for illustration*).\nFurthermore, suppose we need an old version of samtools (0.1.19).\nWe'll select the version using the `=` operator.\n\n","metadata":{}},{"cell_type":"code","source":"conda create -y -p /srv/shiny-server/sample-apps/samtools samtools=0.1.19 ","metadata":{"scrolled":true,"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"Note the directory structure in `/srv/shiny-server/sample-apps/samtools`:","metadata":{}},{"cell_type":"code","source":"ls /srv/shiny-server/sample-apps/samtools","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"`conda list -p` treats that directory as a conda environment, and lists installed packages:","metadata":{}},{"cell_type":"code","source":"conda list -p /srv/shiny-server/sample-apps/samtools","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"### Nested (aka \"stacked\") environments","metadata":{}},{"cell_type":"markdown","source":"Normally, when a conda environment is activated, it replaces the previous environment.\nWe can use the `--stack` option to instead nest the environment so that we have access to packages in both.","metadata":{}},{"cell_type":"code","source":"conda activate --stack /srv/shiny-server/sample-apps/samtools","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"`conda env list` shows only the most recently-activated directory (on the top of the environment \"stack\"):","metadata":{}},{"cell_type":"code","source":"conda env list","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"But both `samtools` and `bwa` are in our PATH:","metadata":{}},{"cell_type":"code","source":"type samtools; type bwa","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"To deactivate the current environment, use `conda deactivate`.\n\nAfter this is executed, we're back to the *bwa* environment (only).\nIf executed a second time, we would be back to the *base* environment.","metadata":{}},{"cell_type":"code","source":"conda deactivate\nconda env list","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"### Sharing environments","metadata":{}},{"cell_type":"markdown","source":"OK, we used *bwa* to cure COVID-19. Way to go!\n\nNow for the important part (in academia): publish our work.\n\nWe'll use `conda env export` to export our current conda environment (*bwa*) to a [YAML](https://en.wikipedia.org/wiki/YAML) file that will record and can be used to recreate our (conda) environment:","metadata":{}},{"cell_type":"code","source":"conda env export","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"Redirect this output to a file to save it (let's call the file `environment.yaml`)","metadata":{}},{"cell_type":"code","source":"conda env export > environment.yml\ncat environment.yml","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"To show that this suffices to recreate the *bwa* environment, we'll delete our current bwa environment... ","metadata":{}},{"cell_type":"code","source":"conda deactivate\nconda env remove -n bwa\nconda env list","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"...and recreate the environment using `conda env create`:","metadata":{}},{"cell_type":"code","source":"conda env create -f environment.yml\nconda env list","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"And there's our *bwa* environment:","metadata":{}},{"cell_type":"code","source":"conda list -n bwa","metadata":{"trusted":true},"execution_count":null,"outputs":[]},{"cell_type":"markdown","source":"### Your turn!\nSearch for a package at [bioconda.io]([Bioconda recipe index](https://bioconda.github.io/conda-recipe_index.html)), [Anaconda.org](https://anaconda.org), or using `conda search`, and try to install it into a new environment:","metadata":{}},{"cell_type":"code","source":"conda create -y -n myenv PACKAGE1 [PACKAGE2...]","metadata":{},"execution_count":null,"outputs":[]},{"cell_type":"code","source":"conda activate myenv","metadata":{},"execution_count":null,"outputs":[]}]} ================================================ FILE: tidyverse/part1/binder/Dockerfile ================================================ FROM rocker/binder:3.6.2 ARG NB_USER ARG NB_UID USER root COPY ./tidyverse/part1/ ${HOME} RUN chown -R ${NB_USER} ${HOME} USER ${NB_USER} ================================================ FILE: tidyverse/part1/index.Rmd ================================================ --- title: "Tidyverse Tibbles and Bits" subtitle: "Bioinformatics Coffee Hour" date: "Mar 2, 2021" author: "Danielle Khost; Brian Arnold" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` # Introduction One of the reasons R is so useful is because many people have built extensions for R in the form of R packages that you can install. These packages may do broad statistical analyses or analyze specific data types (e.g. comparative phylogenetics). This lesson will introduce some data manipulation tools within the [tidyverse](https://www.tidyverse.org), which contains several R packages. These packages include: * tidyr to convert long vs. wide form objects (not covered here) * dplyr to transform and filter data objects, as well as to summarize their contents For an overview of how to manipulate data with tidyverse, this [cheat sheet](https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf) is particularly helpful. To install new packages you have never used before in R, use the command `install.packages()`: ```{r} # NOTE that tidyverse is already installed, this is just to illustrate installation # install.packages("tidyverse") ``` For packages that are already installed, we need to load them every session (i.e. if you restart R). It's good practice to load all packages at the top of your R script. More generally, the [tidyverse style guide](http://style.tidyverse.org) offers some good advice on coding style to help make your code easier to read and write. ```{r} library(tidyverse) ``` # Data tables: wide format vs long format When using tidyverse, you will usually want your data to be "tidy". Tidy data is data where: 1. Every column is variable. 2. Every row is an observation. 3. Every cell is a single value. This is also referred to as **"long format"** (in contrast to "wide format"). Most of the functions in tidyverse assume that your data is in long format; if it isn't, you will want to pre-process it before doing further analysis! Let's load in some data to use: ```{r} housing<-read.csv("https://raw.githubusercontent.com/datasets/house-prices-us/master/data/cities-month.csv", stringsAsFactors=F, strip.white = T) housing=housing[c(1:(length(housing)-3),length(housing))] View(housing) ``` ### Tibbles The tidyverse uses its own version of the data frame (from base R) that is similar but has several properties that make it superior. This object is a **tibble**. Let's make a data frame called 'df1' and change it into a tibble to see what it looks like. ```{r} df1<-data.frame(label=c("rep1", "rep2", "rep3", "rep4"), data=c(23, 34, 15, 19)) tbdf1 <- as_tibble(df1) tbdf1 class(tbdf1) ``` You see here that just printing the tibble to screen displays the data types in each of the columns and the dimensions. Although not apparent with this small dataset, another very handy feature of tibbles is that by default they will only print out the first 10 rows and as many columns as fit in your window. Many packages will accept tibbles instead of data frames with no problems, but if you come across an older package that requires a data frame, it is easy to revert with the `as.data.frame()` function. #### Side Note: %>% One important piece of syntax is the %>% operator, which acts like a Unix pipe in R. This means you can pass the output of one command to another in linear fashion, as opposed to having to use either nested operations or temporary objects. This makes your code much easier to read! --- If we look at the dataset we just downloaded, we can see that it is not in proper long, tidy format. ```{r, echo = TRUE} View(housing) ``` Let's polish it using some tidyr functions so that it is easier to work with. ## Going from wide to long: pivot_longer() The pivot_longer() function takes a tibble (or data frame) and lengthens it, i.e. increases the number of rows and decreases number of columns: ```{r, echo = TRUE} housing_clean <- housing %>% as_tibble %>% pivot_longer(cols=c(-Date, -National.US), names_to="location", values_to="local_index") View(housing_clean) ``` The **cols** argument describes what columns need to be reshaped, i.e. which need to be converted into columns. For us, we want to keep the US national average and the date as columns. We give it a list using the **c** ombine function, and by putting an **-** in front of the column names, we tell it to reshape every column *except* Date and National.US The **names_to** argument gives a name for the new variable (i.e. column) that stores the data that formerly was in the column names. In our case, we make a variable called "location" that holds all the city names. The **values_to** argument gives a name for the new variable (i.e. column) that stores the data that was in the cell values, which for us was the local index values in each city. ## Turning a column into multiple columns: separate() The separate() function separates a single character column into several, splitting on a given regular expression. In the following command we run the function twice, separating two different columns: ```{r, echo = TRUE} housing_clean2 <- housing_clean %>% separate(Date, into=c("year", "month"), extra="drop", remove=F) %>% separate(location, into=c("state", "city"),extra="merge") View(housing_clean2) ``` The first argument is the name of the column in the tibble that we are separating ("location" for the first command, "Date" for the second). The **into** argument gives the names (as a character vector) of the columns that we are separating into, i.e. what the names of the new columns will be. Note that the number of elements in the vector determine how many columns the target column will be split into (in this case, two). The **extra** argument tells the function what to do with the "pieces" that are leftover after splitting. "Merge" tells the function to add the leftover pieces back to the final column; "drop" discards them. To better understand this, we should look at how separate() splits a character column. You might be wondering how separate() "knows" to split our columns on a period ("."). This is because by default, separate() splits on every non-alphanumeric character (e.g. space, period, dash, etc.) given a character vector. This is controlled by the **sep** argument, which we did not included in our code so separate() just uses the default (remember to get the whole list of arguments for a function, you can type ?functionname, e.g. ?separate). Finally, the **remove** argument determines whether the original column is retained or not (defaults to TRUE). So for our data, the original "location" column is discarded and we keep only the split columns, while the original "Date" column is retained along with its children. Now let's put it all together. We could do things one function at a time, as above, but it is much cleaner and easier to read if we create our final, cleaned dataset in a single pipeline: ```{r, echo = TRUE} housing_clean <- housing %>% as_tibble %>% pivot_longer(cols=c(-Date, -National.US), names_to="location", values_to="local_index") %>% separate(Date, c("year", "month"), extra="drop", remove=F) %>% separate(location, c("state", "city"),extra="merge") View(housing_clean) ``` We can observe the differences between housing and housing_clean to see what's being done here, and a more in-depth description of what these functions do can be found in the cheat sheet. ## Subsetting and Manipulating Data with dplyr Dplyr is a package included in tidyverse, and is useful for manipulating our data. Each action gets its own (verb) function -- so for example filtering data by rows is done with the filter() function. All of these functions have a very similar syntax to tidyr functions. arrange(), rename(), select(), mutate() are used to sort data, rename columns, and update/create columns We'll use the housing dataset to look at how these functions work. arrange() sorts by one or more columns, with subsequent columns used to break ties in earlier columns. E.g., ```{r} housing_clean %>% arrange(year, month) housing_clean %>% arrange(city, year) housing_clean %>% arrange(month,state) housing_clean %>% arrange(desc(year)) ``` rename() renames a column: ```{r} housing_clean %>% arrange(year, month, state, city) %>% rename(national_index = National.US) ``` select() selects columns to keep. Note that we can simultaneously rename and reorder columns: ```{r} housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) ``` distinct() can be used to identify all unique values in your call set: ```{r} housing_clean %>% select(state,city) %>% distinct ``` mutate() is an especially useful function that can do a variety of things, usually in combination with other functions. It can create new columns or update existing ones, while keeping everthing else unchanged. For instance, say we want to change our "month" column from the integer value to the three letter abbreviation: ```{r} housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) %>% mutate(month = month.abb[as.integer(month)]) ``` R has several built-in constants, one of which is this "month.abb" function, which has the three letter abbreviations of the months in the Gregorian calendar. What this mutate() call is doing is passing the values of the month column as integers to the month.abb function, which then automatically converts them to their appropriate abbreviations. Let's look at some other things mutate() can do. We can include several mutate calls at once: ```{r} housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) %>% mutate(month = month.abb[as.integer(month)], city=sub(".", "_", city, fixed=TRUE), rel_index=local_index/national_index) ``` This command uses mutate() three times: 1) The first changes the months, as above 2) In the second mutate() call, we are pairing mutate() with the sub() function, which substitutes characters in a string; we are telling substitute to replace all "." characters with a "_" character for the "city" character vector (i.e. our city column) 3) In the third mutate() call, we are using mutate to create an entirely new column called "rel_index", whose value is local_index / national_index Now that we've gone through all the dplyr functions, let's update our nice clean housing dataset. ```{r} housing_clean <- housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) %>% mutate(month = month.abb[as.integer(month)], city=sub(".", "_", city, fixed=TRUE), rel_index=local_index/national_index) ``` ## Filtering functions Sometimes we need to filter datasets, and we can do this by selecting rows that meet logical conditions. We set these up with logical tests. The filter() function can be used to select rows meeting these conditions: ```{r} housing_clean %>% filter(city == "Boston") ``` We can give filter many logical tests, all separated by commas. These are linked by logical and (all must be true to select that row): ```{r} housing_clean %>% filter(city=="Boston", month=="Aug") ``` If we want to use other booleans, just put them in one statement, e.g.: ```{r} housing_clean %>% filter(city=="Boston", month == "Aug" | month == "Jan") ``` Note that we can combine filters and the other statements to get useful subsets, for example let's say we want to see three years when the relative index in Boston was the highest (as of Jan): ```{r} housing_clean %>% filter(city=="Boston", month=="Jan") %>% arrange(desc(rel_index)) %>% select(year,rel_index) %>% head(n=3) ``` Finally, many times you want to filter out missing data (typically indicated by `NA` in R), prior to conducting any analyses. To do this, we an use the `is.na()` fuction, which will return a vector with TRUE if NA is present, and FALSE otherwise. For example, let's identify all rows that are missing the local_index in housing_clean. ```{r} housing_clean %>% filter(is.na(local_index)) ``` To get the inverse for this (or any logical statement), simply add a `!` to the front. ```{r} housing_clean %>% filter(!is.na(local_index)) ``` ================================================ FILE: tidyverse/part1_winter2021/binder/Dockerfile ================================================ FROM rocker/binder:3.6.2 ARG NB_USER ARG NB_UID USER root COPY ./tidyverse/part1/ ${HOME} RUN chown -R ${NB_USER} ${HOME} USER ${NB_USER} ================================================ FILE: tidyverse/part1_winter2021/index.Rmd ================================================ --- title: "Tidyverse Tibbles and Bits" subtitle: "Bioinformatics Coffee Hour" date: "Mar 2, 2021" author: "Danielle Khost; Brian Arnold" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` # Introduction One of the reasons R is so useful is because many people have built extensions for R in the form of R packages that you can install. These packages may do broad statistical analyses or analyze specific data types (e.g. comparative phylogenetics). This lesson will introduce some data manipulation tools within the [tidyverse](https://www.tidyverse.org), which contains several R packages. These packages include: * tidyr to convert long vs. wide form objects (not covered here) * dplyr to transform and filter data objects, as well as to summarize their contents For an overview of how to manipulate data with tidyverse, this [cheat sheet](https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf) is particularly helpful. To install new packages you have never used before in R, use the command `install.packages()`: ```{r} # NOTE that tidyverse is already installed, this is just to illustrate installation # install.packages("tidyverse") ``` For packages that are already installed, we need to load them every session (i.e. if you restart R). It's good practice to load all packages at the top of your R script. More generally, the [tidyverse style guide](http://style.tidyverse.org) offers some good advice on coding style to help make your code easier to read and write. ```{r} library(tidyverse) ``` # Data tables: wide format vs long format When using tidyverse, you will usually want your data to be "tidy". Tidy data is data where: 1. Every column is variable. 2. Every row is an observation. 3. Every cell is a single value. This is also referred to as **"long format"** (in contrast to "wide format"). Most of the functions in tidyverse assume that your data is in long format; if it isn't, you will want to pre-process it before doing further analysis! Let's load in some data to use: ```{r} housing<-read.csv("https://raw.githubusercontent.com/datasets/house-prices-us/master/data/cities-month.csv", stringsAsFactors=F, strip.white = T) housing=housing[c(1:(length(housing)-3),length(housing))] View(housing) ``` ### Tibbles The tidyverse uses its own version of the data frame (from base R) that is similar but has several properties that make it superior. This object is a **tibble**. Let's make a data frame called 'df1' and change it into a tibble to see what it looks like. ```{r} df1<-data.frame(label=c("rep1", "rep2", "rep3", "rep4"), data=c(23, 34, 15, 19)) tbdf1 <- as_tibble(df1) tbdf1 class(tbdf1) ``` You see here that just printing the tibble to screen displays the data types in each of the columns and the dimensions. Although not apparent with this small dataset, another very handy feature of tibbles is that by default they will only print out the first 10 rows and as many columns as fit in your window. Many packages will accept tibbles instead of data frames with no problems, but if you come across an older package that requires a data frame, it is easy to revert with the `as.data.frame()` function. #### Side Note: %>% One important piece of syntax is the %>% operator, which acts like a Unix pipe in R. This means you can pass the output of one command to another in linear fashion, as opposed to having to use either nested operations or temporary objects. This makes your code much easier to read! --- If we look at the dataset we just downloaded, we can see that it is not in proper long, tidy format. ```{r, echo = TRUE} View(housing) ``` Let's polish it using some tidyr functions so that it is easier to work with. ## Going from wide to long: pivot_longer() The pivot_longer() function takes a tibble (or data frame) and lengthens it, i.e. increases the number of rows and decreases number of columns: ```{r, echo = TRUE} housing_clean <- housing %>% as_tibble %>% pivot_longer(cols=c(-Date, -National.US), names_to="location", values_to="local_index") View(housing_clean) ``` The **cols** argument describes what columns need to be reshaped, i.e. which need to be converted into columns. For us, we want to keep the US national average and the date as columns. We give it a list using the **c** ombine function, and by putting an **-** in front of the column names, we tell it to reshape every column *except* Date and National.US The **names_to** argument gives a name for the new variable (i.e. column) that stores the data that formerly was in the column names. In our case, we make a variable called "location" that holds all the city names. The **values_to** argument gives a name for the new variable (i.e. column) that stores the data that was in the cell values, which for us was the local index values in each city. ## Turning a column into multiple columns: separate() The separate() function separates a single character column into several, splitting on a given regular expression. In the following command we run the function twice, separating two different columns: ```{r, echo = TRUE} housing_clean2 <- housing_clean %>% separate(Date, into=c("year", "month"), extra="drop", remove=F) %>% separate(location, into=c("state", "city"),extra="merge") View(housing_clean2) ``` The first argument is the name of the column in the tibble that we are separating ("location" for the first command, "Date" for the second). The **into** argument gives the names (as a character vector) of the columns that we are separating into, i.e. what the names of the new columns will be. Note that the number of elements in the vector determine how many columns the target column will be split into (in this case, two). The **extra** argument tells the function what to do with the "pieces" that are leftover after splitting. "Merge" tells the function to add the leftover pieces back to the final column; "drop" discards them. To better understand this, we should look at how separate() splits a character column. You might be wondering how separate() "knows" to split our columns on a period ("."). This is because by default, separate() splits on every non-alphanumeric character (e.g. space, period, dash, etc.) given a character vector. This is controlled by the **sep** argument, which we did not included in our code so separate() just uses the default (remember to get the whole list of arguments for a function, you can type ?functionname, e.g. ?separate). Finally, the **remove** argument determines whether the original column is retained or not (defaults to TRUE). So for our data, the original "location" column is discarded and we keep only the split columns, while the original "Date" column is retained along with its children. Now let's put it all together. We could do things one function at a time, as above, but it is much cleaner and easier to read if we create our final, cleaned dataset in a single pipeline: ```{r, echo = TRUE} housing_clean <- housing %>% as_tibble %>% pivot_longer(cols=c(-Date, -National.US), names_to="location", values_to="local_index") %>% separate(Date, c("year", "month"), extra="drop", remove=F) %>% separate(location, c("state", "city"),extra="merge") View(housing_clean) ``` We can observe the differences between housing and housing_clean to see what's being done here, and a more in-depth description of what these functions do can be found in the cheat sheet. ## Subsetting and Manipulating Data with dplyr Dplyr is a package included in tidyverse, and is useful for manipulating our data. Each action gets its own (verb) function -- so for example filtering data by rows is done with the filter() function. All of these functions have a very similar syntax to tidyr functions. arrange(), rename(), select(), mutate() are used to sort data, rename columns, and update/create columns We'll use the housing dataset to look at how these functions work. arrange() sorts by one or more columns, with subsequent columns used to break ties in earlier columns. E.g., ```{r} housing_clean %>% arrange(year, month) housing_clean %>% arrange(city, year) housing_clean %>% arrange(month,state) housing_clean %>% arrange(desc(year)) ``` rename() renames a column: ```{r} housing_clean %>% arrange(year, month, state, city) %>% rename(national_index = National.US) ``` select() selects columns to keep. Note that we can simultaneously rename and reorder columns: ```{r} housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) ``` distinct() can be used to identify all unique values in your call set: ```{r} housing_clean %>% select(state,city) %>% distinct ``` mutate() is an especially useful function that can do a variety of things, usually in combination with other functions. It can create new columns or update existing ones, while keeping everthing else unchanged. For instance, say we want to change our "month" column from the integer value to the three letter abbreviation: ```{r} housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) %>% mutate(month = month.abb[as.integer(month)]) ``` R has several built-in constants, one of which is this "month.abb" function, which has the three letter abbreviations of the months in the Gregorian calendar. What this mutate() call is doing is passing the values of the month column as integers to the month.abb function, which then automatically converts them to their appropriate abbreviations. Let's look at some other things mutate() can do. We can include several mutate calls at once: ```{r} housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) %>% mutate(month = month.abb[as.integer(month)], city=sub(".", "_", city, fixed=TRUE), rel_index=local_index/national_index) ``` This command uses mutate() three times: 1) The first changes the months, as above 2) In the second mutate() call, we are pairing mutate() with the sub() function, which substitutes characters in a string; we are telling substitute to replace all "." characters with a "_" character for the "city" character vector (i.e. our city column) 3) In the third mutate() call, we are using mutate to create an entirely new column called "rel_index", whose value is local_index / national_index Now that we've gone through all the dplyr functions, let's update our nice clean housing dataset. ```{r} housing_clean <- housing_clean %>% arrange(year, month, state, city) %>% select(year, month, city, state, local_index, national_index = National.US) %>% mutate(month = month.abb[as.integer(month)], city=sub(".", "_", city, fixed=TRUE), rel_index=local_index/national_index) ``` ## Filtering functions Sometimes we need to filter datasets, and we can do this by selecting rows that meet logical conditions. We set these up with logical tests. The filter() function can be used to select rows meeting these conditions: ```{r} housing_clean %>% filter(city == "Boston") ``` We can give filter many logical tests, all separated by commas. These are linked by logical and (all must be true to select that row): ```{r} housing_clean %>% filter(city=="Boston", month=="Aug") ``` If we want to use other booleans, just put them in one statement, e.g.: ```{r} housing_clean %>% filter(city=="Boston", month == "Aug" | month == "Jan") ``` Note that we can combine filters and the other statements to get useful subsets, for example let's say we want to see three years when the relative index in Boston was the highest (as of Jan): ```{r} housing_clean %>% filter(city=="Boston", month=="Jan") %>% arrange(desc(rel_index)) %>% select(year,rel_index) %>% head(n=3) ``` Finally, many times you want to filter out missing data (typically indicated by `NA` in R), prior to conducting any analyses. To do this, we an use the `is.na()` fuction, which will return a vector with TRUE if NA is present, and FALSE otherwise. For example, let's identify all rows that are missing the local_index in housing_clean. ```{r} housing_clean %>% filter(is.na(local_index)) ``` To get the inverse for this (or any logical statement), simply add a `!` to the front. ```{r} housing_clean %>% filter(!is.na(local_index)) ``` ================================================ FILE: tidyverse/part2/binder/Dockerfile ================================================ FROM rocker/binder:3.6.2 ARG NB_USER ARG NB_UID USER root COPY ./tidyverse/part2/ ${HOME} RUN chown -R ${NB_USER} ${HOME} USER ${NB_USER} ================================================ FILE: tidyverse/part2/index.Rmd ================================================ --- title: "Tidyverse Tibbles and Bits" subtitle: "Bioinformatics Coffee Hour" date: "May 11 2020" author: "Brian Arnold" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` ### Making data tidy with tidyr The goal of tidyr is to convert between 'wide' data and 'long' data. Long data is tidy data: each row is an observation, each column is a variable. Wide data has many columns for the same variable, one for each level of a classification variable. For example, we will use some data on M&Ms to illustrate the differences between these two different formats and why you should care about knowing how to convert between them. ```{r} library(tidyverse) mms_wide <-read.table("http://www.randomservices.org/random/data/MM.txt", header=TRUE,stringsAsFactors=F) mms_wide$BagID = seq(1:length(mms_wide$Red)) ``` mms_wide is in wide format: there are many columns for the various colors. This data would be more tidy if instead we had 1.) a column representing color as a variable that could take on one of the 6 values and 2.) a corresponding column that contained the count data for each of these colors. We can use the pivot_longer() function to convert from wide format to long format: ```{r} mms_long <- mms_wide %>% as_tibble %>% pivot_longer(cols=c(-Weight, -BagID), names_to="color", values_to="count") %>% arrange(BagID,color) ``` We need to select which columns to use for this operation. One way to do this would be to list all the columns. An alternative way (that we used here) would be to list all the columns you DO NOT want to use by preceding their names with "-". However, say we inherited our data table in long format but would like it in wide format, we may also convert our data from long back to (the original) wide format using pivot_wider(): ```{r} mms_wide2 <- mms_long %>% pivot_wider(names_from="color", values_from="count") ``` While it may sound pointless at first to transform our data table in a way that makes it have either more columns or rows (at least it did to me!), it actually is extremely useful for analyzing features of these data using relatively little code. To illustrate, let's use the 'summarize' function from dplyr. Say we wanted to know the total number of M&Ms in the dataset. In wide format, we'd have to go across all the columns, one for each color. In long format, these data are now in a single column named 'count': ```{r} mms_long %>% summarize(total_mms=sum(count)) ``` However, it gets even more interesting when we also use the group_by() function (also from dplyr), which is able to communicate to the summarize function to tell it how to summarize the data. For instance, group_by is able to analyze our data as a function of the "color" variable we created when we converted to long format. Here, we can see how many observations there are per color. ```{r} mms_long %>% group_by(color) %>% summarize( mms_per_color=sum(count) ) ``` Note 1: the summarize function outputs a new tibble, which contains a new column we created (named 'mms_per_color') that contains the summed count data. You can continue to do calculations on this tibble (for example, calculate the variance of colors), but you cannot call your original tibble again within the same set of pipes. Note 2: we could have also done this by hand with the data in wide format, summing all the values underneath a column that corresponds to 'Red', 'Blue', etc... but it would be much more tedious and involve more code. Using less code is preferable because it can get difficult to read and understand code that is unecessarily long, especially if it involves many complex procedures. Writing clean code can also help the author understand their own code faster when they come back to it after not having thought about it for months (or however long it takes reviews to come back from a journal!). Instead of summing over all bags of M&Ms for a each color, we can use the mean() function within summarize() to get the mean number of M&Ms for a each color. ```{r} mms_long %>% group_by(color) %>% summarize( mean_mms_per_color=mean(count) ) ``` Let's instead combine the group_by function with mutate. Here, mutate() creates a new column for the current tibble, instead of the summarize() function above which creates an entirely new tibble. Let's compute the fraction of each color in each bag and store this in a new column: ```{r} mms_long %>% group_by(BagID) %>% mutate( percent = 100*(count/sum(count)) ) ``` Note there is some subtle stuff going on here. The group_by function communicates to the mutate function. Here we use mutate on a grouped tibble (by BagID), so operations within the mutate function like sum() work **within the grouping**. So sum(count) computes the group-wise sum, which means it is easy to get frequencies or percents. To more easily visualize these data (all percentages for a particular BagID on the same line/row), lets convert it to wide format just for printing to screen: ```{r} mms_long %>% group_by(BagID) %>% mutate(percent = 100*(count/sum(count))) %>% pivot_wider(names_from=color, values_from=percent) ``` We can then use this insight to explore the data. For example, let's get the 5 bags of M&Ms with the highest percentage of red candies, conditioning on a weight of at least 47g: ```{r} mms_long %>% group_by(BagID) %>% mutate(percent = 100*(count/sum(count))) %>% select(-count) %>% filter(color == "Red", Weight > 47) %>% arrange(desc(percent)) %>% head(n=5) ``` ## Bonus material Let's do a problem to help commit these concepts to memory (and to get used to thinking of which functions we need based on how a problem/question is phrased). We will reload the housing dataset from before, and processes it in a few ways that I'll skip for the moment: ```{r} housing<-read.csv("https://raw.githubusercontent.com/datasets/house-prices-us/master/data/cities-month.csv", stringsAsFactors=F, strip.white = T) housing=housing[c(1:(length(housing)-3),length(housing))] housing_clean <- housing %>% as_tibble %>% pivot_longer(cols=c(-Date, -National.US), names_to="location", values_to="local_index") %>% separate(location, c("state", "city"),extra="merge") %>% separate(Date, c("year", "month"), extra="drop", remove=F) %>% select(year, month, city, state, local_index, national_index=National.US) %>% arrange(year, month, state, city) %>% mutate(year = as.integer(year), month = month.abb[as.integer(month)], city = sub(".", "_", city, fixed=TRUE), rel_index = local_index/national_index) ``` ### Exercises with summarize and group_by ### Problem Using the housing_clean dataset, find the three cities with the highest relative index in February, averaged across the years. Get rid of missing data for this analysis too! 1.) This question is asking us to analyze **cities**, so we should probably group by that variable. 2.) It only wants us to consider February, so we should probably filter on that. While we're doing that, we might as well filter on missing data too using !is.na(). 3.) We want to study the *averaged* relative index for each city, so we can probably put that variable into the summarize function with mean(). 4.) It also asks us for the cities with the 3 highest mean relative indices, so lets just arrange our results by the mean relative index and peek at the first 3 ```{r, echo=TRUE} housing_clean %>% filter(month=="Feb", !is.na(rel_index)) %>% group_by(city) %>% summarize(mean_rel_index = mean(rel_index)) %>% arrange(desc(mean_rel_index)) %>% head(n=3) ``` Let's understand how this works more by breaking it :). Let's switch around the functions to see why their order is important. Let's use the filter function second, after grouping by city: ```{r, echo=TRUE} housing_clean %>% group_by(city) %>% filter(month=="Feb", !is.na(rel_index)) %>% summarize(mean_rel_index = mean(rel_index)) %>% arrange(desc(mean_rel_index)) %>% head(n=3) ``` We get the same results! How about if we move the filter statement down again, making it the third operation: ```{r, echo=TRUE} housing_clean %>% group_by(city) %>% summarize(mean_rel_index = mean(rel_index)) %>% filter(month=="Feb", !is.na(rel_index)) %>% arrange(desc(mean_rel_index)) %>% head(n=3) ``` This breaks because at the point summarize() is used, we've created a new tibble with two columns: city and mean_rel_index, which is an average across all months and all years for each city (because we grouped by city). Since we had not filtered on the particular month of February by that part of the code, the mean is now calculated for all data so these numbers will be different than before (and not what the question asked for!). Then, when we try to filter on the month of February, our tibble at that point no onger has a column corresponding to months! This breaking emphasizes that we cannot just selecting a bunch of operations that we want to do and expect R to figure everything out. Because of the use of pipes and the flow of information, the sequence of operations matters here. We must use logic and our understanding of these functions (that summarize produces a new tibble) to figure out what order of operations is needed to get what we want. Try running each line of the code, starting with the first 2 lines, then the first 3 lines etc to see exactly where the code breaks. This will give us an idea of how to fix our code! ================================================ FILE: tidyverse/part2_winter2021/binder/Dockerfile ================================================ FROM rocker/binder:3.6.2 ARG NB_USER ARG NB_UID USER root COPY ./tidyverse/part2_winter2021/ ${HOME} RUN chown -R ${NB_USER} ${HOME} USER ${NB_USER} ================================================ FILE: tidyverse/part2_winter2021/mask-use-by-county.csv ================================================ COUNTYFP,NEVER,RARELY,SOMETIMES,FREQUENTLY,ALWAYS 01001,0.053,0.074,0.134,0.295,0.444 01003,0.083,0.059,0.098,0.323,0.436 01005,0.067,0.121,0.12,0.201,0.491 01007,0.02,0.034,0.096,0.278,0.572 01009,0.053,0.114,0.18,0.194,0.459 01011,0.031,0.04,0.144,0.286,0.5 01013,0.102,0.053,0.257,0.137,0.451 01015,0.152,0.108,0.13,0.167,0.442 01017,0.117,0.037,0.15,0.136,0.56 01019,0.135,0.027,0.161,0.158,0.52 01021,0.06,0.07,0.058,0.194,0.618 01023,0.049,0.038,0.126,0.219,0.568 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56003,0.182,0.187,0.12,0.257,0.253 56005,0.216,0.164,0.131,0.201,0.289 56007,0.044,0.137,0.087,0.278,0.454 56009,0.138,0.113,0.121,0.293,0.335 56011,0.145,0.115,0.143,0.192,0.404 56013,0.16,0.062,0.06,0.294,0.424 56015,0.201,0.169,0.111,0.223,0.296 56017,0.208,0.093,0.068,0.307,0.324 56019,0.183,0.208,0.103,0.216,0.29 56021,0.143,0.127,0.1,0.221,0.409 56023,0.131,0.183,0.128,0.235,0.324 56025,0.1,0.084,0.094,0.325,0.398 56027,0.169,0.191,0.177,0.222,0.241 56029,0.189,0.153,0.191,0.205,0.262 56031,0.149,0.149,0.123,0.16,0.418 56033,0.17,0.251,0.099,0.203,0.278 56035,0.223,0.111,0.061,0.231,0.374 56037,0.061,0.295,0.23,0.146,0.268 56039,0.095,0.157,0.16,0.247,0.34 56041,0.098,0.278,0.154,0.207,0.264 56043,0.204,0.155,0.069,0.285,0.287 56045,0.142,0.129,0.148,0.207,0.374 ================================================ FILE: tidyverse/part2_winter2021/tidyverse_part2_winter2021.Rmd ================================================ --- title: "Tidyverse Tibbles and Bits" subtitle: "Bioinformatics Coffee Hour" date: "Mar 9, 2021" author: "Brian Arnold; Danielle Khost" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` This is the second session covering tidyverse commands. We will show you how to use some new functions, but we will also use some of the functions we previously used last week which will be good for extra practice. If you missed the previous session, that should not be a problem. From last time, we looked at this [cheat sheet](https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf) to occasionally guide us. It's typical to load all the libraries you need at the top of your code. ```{r} library(tidyverse) ``` ------ For this week, rather than use an example dataset, we are going to use several publically available datasets from the NYT github repository on the COVID-19 pandemic. This is because "wild-caught" datasets are often much messier than example datasets, so it will help us become more familiar with how you would actually go about processing data. We are going to keep our analysis purely descriptive, as it would be irresponsible to make any conclusive claims after fiddling around with R for only an hour! ## Dataset 1: mask useage Let's load in the first data set on mask usage in the US, which is in the form of a comma separated list. This data is from a NYT survey where they asked the question: "How often do you wear a mask in public when you expect to be within six feet of another person?" (As a note, this survey was done several months ago, so these numbers are not the most up-to-date.) ```{r} <<<<<<< HEAD mask_use_wide <- read_delim(file="mask-use-by-county.csv", delim=",") ======= mask_use_wide <- read_delim(file="https://raw.githubusercontent.com/nytimes/covid-19-data/bde13b021e99c6b4a63fb66a6144e889cc635e31/mask-use/mask-use-by-county.csv", delim=",") >>>>>>> bfb375bc22239322d6e6089bdac1ac5be52372c1 mask_use_wide ``` It looks like for all the counties are represented with a 5-digit FIPS code, which is not exactly ideal because we don't know what these numbers mean. We'll get the names in a moment by combining these data with another file that also has these FIPS codes AND county names. We can thus match these data tables based on shared FIPS codes to combine them. ## Dataset 2: US case counts In addition to the mask data, let's also load in data on COVID-19 case counts across the US, also supplied by the NYT, and combine it with our mask use data. Unlike the previous data that we loaded in from our local file system, these data we will directly download because they are so large. This will also show how you can specify web addresses instead of file names for read_delim(). This file is also comma separated, so we will let read_delim() know this. ```{r, include=FALSE} cases <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/master/us-counties.csv", delim=",") %>% arrange(fips) ``` <<<<<<< HEAD This is a lot of data. For ~3000 US counties, there's an estimate of cumulative COVID-19 cases and deaths for each day since last March (it's now March again!!!). For now, let's just look at the start of this month. ======= This is a lot of data. For ~3000 US counties, there's an estimate of cumulative COVID-19 cases and deaths for each day since last March (it's now March again!!!). For now, let's just look at the start of this month. >>>>>>> bfb375bc22239322d6e6089bdac1ac5be52372c1 ```{r, include=FALSE} cases_latest <- cases %>% filter(date == "2021-03-01") %>% arrange(fips) ``` As a refresher, we are using the filter() function to select only the rows that match the string "2021-03-01" in the date column, then using the arrange() function to sort the counties by FIPS code. ----- ## Dataset 3: census data Let's load in some additional data on population size by county, which are obtained from www.census.gov. ```{r, include=FALSE} pop_sizes <- read_delim(file="co-est2019-annres.csv.xz", delim=",") ``` When I'm working with larger files and I want to make sure they looks like I expect them to, instead of opening up the file and scrolling through all the lines, checking each by eye, I typically use various commands to just get an idea of what it looks like before proceeding. Oftentimes, this is sufficient; we don't need to check every line. These are just a few commands I might use to look at the first and last few lines, how many rows there are overall, and also get an idea of how much missing data there might be: ```{r} head(pop_sizes) nrow(pop_sizes) sum(!is.na(pop_sizes$'2019')) tail(pop_sizes) ``` Ok so these data are a little messy and need to be cleaned up. From these commands, we can see that the county names are all preceded with a ".", something we'll deal with in a moment. The number of rows (i.e. the number of counties) in the table is very similar to what we expect (3,142 according to wikipedia), but maybe larger by 7 rows or so. We can see that the entire US was included in this table, which is not a county so we should remove that. Looking at the bottom of the table, we see some footnotes were left in. These are also contributing to the extra number of rows and should absolutely be removed. Let's remove these rows at the beginning and end with the **slice()** function, which pulls out rows from a data frame/tibble based on their ordinal position. It keeps all the columns, in contrast to the select() function that we discussed last week. ```{r, include=FALSE} pop_sizes <- pop_sizes %>% dplyr::slice(2:3143) ``` With this command, we are slicing out the middle of the data frame to remove the row with data from the whole US, as well as the footnotes at the bottom. The 'Geographic Area' column has two pieces of info in it: county and state. We should split this column into two columns so that we can separately access the info. For instance, later on we will do an analysis not by county but by state, summing up across a state's counties, and this requires having this information in it's own separate column. To separate this info into 2 seperate columns, we will use the separate() function: ```{r, include=FALSE} pop_sizes <- pop_sizes %>% separate(col = 'Geographic Area', into = c("County", "State"), sep = ", ") ``` Note that unlike last time we are specifying what we want to separate on with the "sep=" argument, which is a comma followed by a white space. Let's get rid of extra characters in the new 'County' column. We don't need the "." that precedes each name, and it's pointless that each individual county name is followed by "County"... we know they're counties based on the name of the column. We can use the mutate() function to add new rows with new names, but if the name of the new row we want is the same as an existing one, it just replaces it! ```{r, include=FALSE} pop_sizes <- pop_sizes %>% mutate(County = str_remove(string = County,pattern = ".")) %>% mutate(County = str_remove(string = County,pattern = " County")) ``` We are combining the mutate() function with **str_remove()** which, as the name suggests, will remove a given string that matches a pattern from a character column, the County column in this case. This table includes population size data for quite a few years. Let's just get the most recent population estimate, selecting the 2019 column with the select() function, which as mentioned above pulls out specific columns: ```{r, include=FALSE} pop_sizes <- pop_sizes %>% select(County, State, "2019") ``` As we did above for the data sets we used last week, we can also combine all of these individual commands into one compact command that might look something like this: ```{r, include=FALSE} pop_sizes <- read_delim(file="co-est2019-annres.csv.xz", delim=",") %>% slice(2:3143) %>% separate(col = 'Geographic Area', into = c("County", "State"), sep = ", ") %>% mutate(County = str_remove(string = County,pattern = ".")) %>% mutate(County = str_remove(string = County,pattern = " County")) %>% select(County, State, "2019") ``` We can take a quick peek at these population size data, now that they're cleaned, using the summary() function, which shows there's a ton of variability in county sizes! ```{r} summary(pop_sizes) ``` ## Merging data sets with inner_join() Let's combine these data on population sizes with our previous data on case counts and mask usage, as it may reveal interesting dynamics that vary by county size. We will first use the join command on the case count data and the county population size data, joining by BOTH county and state. There are many different types of joining functions and it can get fairly confusing, so today we will focus just on **inner_join**. ```{r, include=FALSE} cases_popsize <- inner_join(x=cases_latest, y=pop_sizes, by=c("county"="County", "state"="State")) ``` The "x=" and "y=" arguments specify which datasets we want to merge. Inner_join() returns all rows in dataset x that has matching values in dataset y, and all the columns in both x and y. The "by=" arugment tells what variables the function should merge by, as a character vector. In other words we tell the function what columns we want to match: it compares the values in the columns in x and y, and if they match it merges them. We are matching the "county" coumn in cases_latest with the "County" column in pop_sizes, and the "state" and "State" columns in the same. Note that because the column names (i.e. variable names) are slightly different between datasets, we have to specify which we are matching using an "="; if they had the same names, we could just list them. ## More merging Now let's merge this table that has case counts and population size with the mask data! For now, let's not add in all the mask data. Let's only incorporate the the proportion of people who are "ALWAYS" masked for each county. ```{r, include=FALSE} cases_popsize_masked <- inner_join(x=cases_popsize, y=select(mask_use_wide, c(COUNTYFP, ALWAYS)), by=c("fips" = "COUNTYFP")) ``` Note that we are nesting the select() function within our inner_join() call, allowing us to subset the mask useage dataset without making an entirely new data frame. Looking at this data table, for each county we now have cases, deaths, population size, and the proportion of people who are always masked. You may also notice the population size data had a column entitled "2019" for size estimates during that year. Let's make this more informative and change it to "pop_size", and rename the ALWAYS column so that we know it's referring to always masked. ```{r, include=FALSE} cases_popsize_masked <- cases_popsize_masked %>% rename("pop_size" = "2019", "AlwaysMasked" = ALWAYS) ``` ## Visualizing data Let's quickly probe these data just to get an idea of what they look like. The main goal here is to teach you how to use R/tidyverse commands to quickly and easily explore your data. Again, due to the context of these data, let's keep any results as fairly descriptive observations. We can't say anything conclusive without more complicated analyses that we'll leave to the public health officials. Let's assume these case count data are accurately measuring the number of people who are getting coronavirus infections (which is obviously an underestimate, as many cases can be asymptomatic!). Assuming this, what fraction of a county's population is testing positive, and how does this fraction vary with population size? Let's use the mutate() function to add a new column with number of cases normalized by population size: ```{r} cases_popsize_masked <- cases_popsize_masked %>% mutate(FracPos = cases/pop_size) ``` Do counties with large populations have a higher proportion of case counts? Let's use a simple plotting function to plot population size on the x axis and proportion of cases on y axis. Since we will look at the proportion of cases by dividing the data in the case counts column by the data in the population size column, let's just make a new column called 'FracPos' that contains this information We can make a quick plot of these data using a base R (i.e. not tidyverse) plotting function: ```{r} cases_popsize_masked <- cases_popsize_masked %>% mutate(FracPos = cases/pop_size) plot(x=cases_popsize_masked$pop_size, y=cases_popsize_masked$FracPos, log="x", ylab="Fraction of cases", xlab="pop size") # DON'T RUN CORRELATION ANALYSES WITH HETEROSCEDASTICITY ``` Looks interesting. Counties with larger population sizes may look like they have higher proportion of cases, but it's a little complicated because there's a lot of variability for counties with smaller population sizes. One thing we might be interested in are those outliers with very high rates of positive cases. What counties are these, and in what states? We can easily check this with the following: ```{r} cases_popsize_masked %>% filter(FracPos > 0.08) %>% arrange(desc(FracPos)) ``` Let's add another column for death rate, and visualize the relationship between the fraction of positive cases and the death rate: ```{r} cases_popsize_masked <- cases_popsize_masked %>% mutate(FracDeaths = deaths/cases) plot(x=cases_popsize_masked$FracPos, y=cases_popsize_masked$FracDeaths, log="", xlab="FracCases", ylab="FracDeaths") ``` What are these counties that have been severely impacted by deaths? Is this just noise from counties with small sizes and/or small case counts? ```{r} cases_popsize_masked %>% filter(FracDeaths > 0.06) %>% arrange(desc(FracDeaths)) ``` # Using group_by() and summarize() functions to evaluate data The use of the group_by() and summarize() functions allows us to quickly get extremely informative information from our data. We'll show how they're useful in many ways, but first we need to do a little more reshaping of our data. Let's go back to our original mask use data table, not the combined one. What if we wanted to quickly get the overall mean values of ALWAYS, ... , NEVER across all the counties? We could use the original data in wide format, and calculate the mean for each of these columns. We could also use the simple summary() function which outputs a table: ```{r} mean(mask_use_wide$NEVER) mean(mask_use_wide$RARELY) mean(mask_use_wide$SOMETIMES) mean(mask_use_wide$FREQUENTLY) mean(mask_use_wide$ALWAYS) summary(mask_use_wide) ``` You can do it with way, but it is kind of clunky, and it wouldn't be practical if your dataset has too many variables. It is easier to first convert the data in long format: ```{r, include=FALSE} mask_use_long <- mask_use_wide %>% pivot_longer(cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") %>% rename("fips" = COUNTYFP) %>% arrange(fips) ``` As a reminder, the "cols=" argument specifies which columns we are reshaping into rows (all *except* the "COUNTYFP" column), the "names_to=" argument specifies the name of the column that will hold the names of the reshaped columns, and the "values_to=" argument gives the name of the column that will hold the values of the reshaped columns. We can now use the **group_by()** function, which takes our existing dataset and converts it to a grouped dataset based on a given variable, and combine it with the **summarize()** function (different from summary used above!) to get mean for each group: ```{r} mask_use_long %>% group_by(MaskUseResponse) %>% summarize(mean(MaskUseProportion)) ``` ## More data analysis and visualization Let's explore another example of group_by() and summarize() using our tibble that has all our combined information. Instead of focusing on counties as our unit of analysis, we can use group_by() to easily switch to a state level analysis by telling the summarize() function to perform analyses for each state, combining all the rows that have the same value under the "state" column. When we combine the group_by() function with summarize(), we can easily do some pretty powerful analyses that would take a lot of effort using other programs. ```{r} cases_popsize_masked %>% group_by(state) %>% summarize(MeanMasked = mean(AlwaysMasked)) %>% arrange(desc(MeanMasked)) ``` This is potentially a bad analysis because we are calculating a mean for a state based on it's counties, and some of these counties may be represented by fewer people. So if we truly wanted the average behavior of a state, we should weight each county by its population size. Why should a county with a size of 400 contribute equally to the mean as a county of size 40,000? The following code calculates a custom mean value, where each county is weighted by its population size: ```{r} cases_popsize_masked %>% group_by(state) %>% summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>% arrange(desc(WeightedMeanMasked)) ``` Did this weighting by county size actually make a difference? Let's store these analyses as 'x' and 'y' and compare them: ```{r} x <- cases_popsize_masked %>% group_by(state) %>% summarize(MeanMasked = mean(AlwaysMasked)) %>% arrange(state) y <- cases_popsize_masked %>% group_by(state) %>% summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>% arrange(state) hist(x$MeanMasked - y$WeightedMeanMasked) ``` It looks like on average, AlwaysMasked estimates that don't take pop_size into account are systematically lower than those that do. When we don't take county size into account, we effectively give more weight to smaller counties. # Summary: ## New tidyverse functions used today: - slice() to select specific rows by index number - inner_join() to merge datasets based on given variable(s) - mutate() with str_remove to get rid of characters or words we don't want, there are many similar functions in the stringr package, which has its own cheat sheet - group_by() to categorize our data by the values in a particular column (here, by state) - summarize() to calculate simple but very informative statistics, such as mean and variance ================================================ FILE: tidyverse/part2_winter2021/tidyverse_part2_winter2021.nb.html ================================================ R Notebook

NEW FXNS: slice(), inner_join(), summarize(), group_by()

This is the second session covering tidyverse commands. We will show you how to use some new functions, but we will also use some of the functions we previously used last week which will be good for extra practice. If you missed the previous session, that should not be a problem.

From last time, we looked at this cheat sheet to occasionally guide us.

It’s typical to load all the libraries you need at the top of your code.

library(tidyverse)

For this week, rather than use an example dataset, we are going to use several publically available datasets from the NYT github repository on the COVID-19 pandemic. This is because “wild-caught” datasets are often much messier than example datasets, so it will help us become more familiar with how you would actually go about processing data. We are going to keep our analysis purely descriptive, as it would be irresponsible to make any conclusive claims after fiddling around with R for only an hour!

Dataset 1: mask useage

Let’s load in the first data set on mask usage in the US, which is in the form of a comma separated list. This data is from a NYT survey where they asked the question: “How often do you wear a mask in public when you expect to be within six feet of another person?” (As a note, this survey was done several months ago, so these numbers are not the most up-to-date.)

mask_use_wide <- read_delim(file="~/Documents/GitHub/bioinformatics-coffee-hour/tidyverse/part2_winter2021/mask-use-by-county.csv", delim=",")
Parsed with column specification:
cols(
  COUNTYFP = col_character(),
  NEVER = col_double(),
  RARELY = col_double(),
  SOMETIMES = col_double(),
  FREQUENTLY = col_double(),
  ALWAYS = col_double()
)
mask_use

It looks like for all the counties are represented with a 5-digit FIPS code, which is not exactly ideal because we don’t know what these numbers mean. We’ll get the names in a moment by combining these data with another file that also has these FIPS codes AND county names. We can thus match these data tables based on shared FIPS codes to combine them.

Dataset 2: US case counts

In addition to the mask data, let’s also load in data on COVID-19 case counts across the US, also supplied by the NYT, and combine it with our mask use data. Unlike the previous data that we loaded in from our local file system, these data we will directly download because they are so large. This will also show how you can specify web addresses instead of file names for read_delim(). This file is also comma separated, so we will let read_delim() know this.

This is a lot of data. For ~3000 US counties, there’s an estimate of cumulative COVID-19 cases and deaths for each day since late January (it’s now March again!!!). For now, let’s just look at the start of this month.

As a refresher, we are using the filter() function to select only the rows that match the string “2021-03-01” in the date column, then using the arrange() function to sort the counties by FIPS code.


Dataset 3: census data

Let’s load in some additional data on population size by county, which are obtained from www.census.gov.

When I’m working with larger files and I want to make sure they looks like I expect them to, instead of opening up the file and scrolling through all the lines, checking each by eye, I typically use various commands to just get an idea of what it looks like before proceeding. Oftentimes, this is sufficient; we don’t need to check every line.

These are just a few commands I might use to look at the first and last few lines, how many rows there are overall, and also get an idea of how much missing data there might be:

head(pop_sizes) 
nrow(pop_sizes) 
[1] 3149
sum(!is.na(pop_sizes$'2019')) # how many counties have size estimates for 2019? it's even closer to the 3142 we expect!
[1] 3143
tail(pop_sizes)

Ok so these data are a little messy and need to be cleaned up.

From these commands, we can see that the entire US was included in this table, which is not a county. We should remove that. Also, county names are all preceded with a “.”, something we’ll deal with in a moment. The number of rows in the table is very similar to what we expect (3,142 according to wikipedia), but maybe larger by 7 rows or so. The row containing the entire US contributes to this excess of rows beyond our expectation.

Looking at the bottom of the table, we see some footnotes were left in. These are probably contributing to the extra number of rows and should absolutely be removed.

Let’s remove these rows at the beginning and end with the slice() function, which pulls out rows from a data frame/tibble based on their ordinal position. It keeps all the columns, in contrast to the select() function that we discussed last week.

With this command, we are slicing out the middle of the data frame to remove the row with data from the whole US, as well as the footnotes at the bottom.

The ‘Geographic Area’ column has two pieces of info in it: county and state. We should split this column into two columns so that we can separately access the info. For instance, later on we will do an analysis not by county but by state, summing up across a state’s counties, and this requires having this information in it’s own separate column. To separate this info into 2 seperate columns, we will use the separate() function:

Note that unlike last time we are specifying what we want to separate on with the “sep=” argument, which is a comma followed by a white space.

Let’s get rid of extra characters in the new ‘County’ column. We don’t need the “.” that precedes each name, and it’s pointless that each individual county name is followed by “County”… we know they’re counties based on the name of the column. We can use the mutate() function to add new rows with new names, but if the name of the new row we want is the same as an existing one, it just replaces it!

We are combining the mutate() function with str_remove() which, as the name suggests, will remove a given pattern from a character column, the County column in this case.

This table includes population size data for quite a few years. Let’s just get the most recent population estimate, selecting the 2019 column with the select() function, which as mentioned above pulls out specific columns:

As we did above for the data sets we used last week, we can also combine all of these individual commands into one compact command that might look something like this:

We can take a quick peek at these population size data, now that they’re cleaned, using the summary() function, which shows there’s a ton of variability in county sizes!

summary(pop_sizes)
    County             State                2019         
 Length:3142        Length:3142        Min.   :      86  
 Class :character   Class :character   1st Qu.:   10902  
 Mode  :character   Mode  :character   Median :   25726  
                                       Mean   :  104468  
                                       3rd Qu.:   68073  
                                       Max.   :10039107  

Merging data sets with inner_join()

Let’s combine these data on population sizes with our previous data on case counts and mask usage, as it may reveal interesting dynamics that vary by county size.

We will first use the join command on the case count data and the county population size data, joining by BOTH county and state. There are many different types of joining functions and it can get fairly confusing, so today we will focus just on inner_join.

The “x=” and “y=” arguments specify which datasets we want to merge. Inner_join() returns all rows in dataset x that has matching values in dataset y, and all the columns in both x and y.

The “by=” arugment tells what variables the function should merge by, as a character vector. In other words we tell the function what columns we want to match: it compares the values in the columns in x and y, and if they match it merges them. We are matching the “county” coumn in cases_latest with the “County” column in pop_sizes, and the “state” and “State” columns in the same.

Note that because the column names (i.e. variable names) are slightly different between datasets, we have to specify which we are matching using an “=”; if they had the same names, we could just list them.

More merging

Now let’s merge this table that has case counts and population size with the mask data! For now, let’s not add in all the mask data. Let’s only incorporate the the proportion of people who are “ALWAYS” masked for each county.

Note that we are nesting the select() function within our inner_join() call, allowing us to subset the mask useage dataset without making an entirely new data frame.

Looking at this data table, for each county we now have cases, deaths, population size, and the proportion of people who are always masked.

You may also notice the population size data had a column entitled “2019” for size estimates during that year. Let’s make this more informative and change it to “pop_size”, and rename the ALWAYS column so that we know it’s referring to always masked.

Visualizing data

Let’s quickly probe these data just to get an idea of what they look like. The main goal here is to teach you how to use R/tidyverse commands to quickly and easily explore your data. Again, due to the context of these data, let’s keep any results as fairly descriptive observations. We can’t say anything conclusive without more complicated analyses that we’ll leave to the public health officials.

Let’s assume these case count data are accurately measuring the number of people who are getting coronavirus infections (which is obviously an underestimate, as many cases can be asymptomatic!). Assuming this, what fraction of a county’s population is testing positive, and how does this fraction vary with population size? Let’s use the mutate() function to add a new column with number of cases normalized by population size:

cases_popsize_masked <- cases_popsize_masked %>%
  mutate(FracPos = cases/pop_size)

Do counties with large populations have a higher proportion of case counts? Let’s use a simple plotting function to plot population size on the x axis and proportion of cases on y axis. Since we will look at the proportion of cases by dividing the data in the case counts column by the data in the population size column, let’s just make a new column called ‘FracPos’ that contains this information

We can make a quick plot of these data using a base R (i.e. not tidyverse) plotting function:

cases_popsize_masked <- cases_popsize_masked %>%
  mutate(FracPos = cases/pop_size)
plot(x=cases_popsize_masked$pop_size,
     y=cases_popsize_masked$FracPos,
     log="x",
     ylab="Fraction of cases",
     xlab="pop size")

# DON'T RUN CORRELATION ANALYSES WITH HETEROSCEDASTICITY

Looks interesting. Counties with larger population sizes may look like they have higher proportion of cases, but it’s a little complicated because there’s a lot of variability for counties with smaller population sizes.

One thing we might be interested in are those outliers with very high rates of positive cases. What counties are theses, and in what states? We can very easily check this with the following:

cases_popsize_masked %>%
  filter(FracPos > 0.08) %>%
  arrange(desc(FracPos))

Let’s add another column for death rate, and visualize the relationship between the fraction of positive cases and the death rate:

cases_popsize_masked <- cases_popsize_masked %>%
  mutate(FracDeaths = deaths/cases)
plot(x=cases_popsize_masked$FracPos,
     y=cases_popsize_masked$FracDeaths,
     log="",
     xlab="FracCases",
     ylab="FracDeaths")

What are these counties that have been severely impacted by deaths? Is this just noise from counties with small sizes and/or small case counts?

cases_popsize_masked %>%
  filter(FracDeaths > 0.06) %>%
  arrange(desc(FracDeaths))

Using group_by() and summarize() functions to evaluate data

The use of the group_by() and summarize() functions allows us to quickly get extremely informative information from our data.

We’ll show how they’re useful in many ways, but first we need to do a little more reshaping of our data.

Let’s go back to our original mask use data table, not the combined one. What if we wanted to quickly get the overall mean values of ALWAYS, … , NEVER across all the counties? We could use the original data in wide format, and calculate the mean for each of these columns. We could also use the simple summary() function which outputs a table:

mean(mask_use_wide$NEVER)
[1] 0.07993953
mean(mask_use_wide$RARELY)
[1] 0.08291852
mean(mask_use_wide$SOMETIMES)
[1] 0.121318
mean(mask_use_wide$FREQUENTLY)
[1] 0.2077247
mean(mask_use_wide$ALWAYS)
[1] 0.5080936
summary(mask_use_wide)
   COUNTYFP             NEVER             RARELY          SOMETIMES        FREQUENTLY    
 Length:3142        Min.   :0.00000   Min.   :0.00000   Min.   :0.0010   Min.   :0.0290  
 Class :character   1st Qu.:0.03400   1st Qu.:0.04000   1st Qu.:0.0790   1st Qu.:0.1640  
 Mode  :character   Median :0.06800   Median :0.07300   Median :0.1150   Median :0.2040  
                    Mean   :0.07994   Mean   :0.08292   Mean   :0.1213   Mean   :0.2077  
                    3rd Qu.:0.11300   3rd Qu.:0.11500   3rd Qu.:0.1560   3rd Qu.:0.2470  
                    Max.   :0.43200   Max.   :0.38400   Max.   :0.4220   Max.   :0.5490  
     ALWAYS      
 Min.   :0.1150  
 1st Qu.:0.3932  
 Median :0.4970  
 Mean   :0.5081  
 3rd Qu.:0.6138  
 Max.   :0.8890  

You can do it with way, but it is kind of clunky, and it wouldn’t be practical if your dataset has too many variables. It is easier to first convert the data in long format:

As a reminder, the “cols=” argument specifies which columns we are reshaping into rows (all except the “COUNTYFP” column), the “names_to=” argument specifies the name of the column that will hold the names of the reshaped columns, and the “values_to=” argument gives the name of the column that will hold the values of the reshaped columns.

We can now use the group_by() function, which takes our existing dataset and converts it to a grouped dataset based on a given variable, and combine it with the summarize() function (different from summary used above!) to get mean for each group:

mask_use_long %>% 
  group_by(MaskUseResponse) %>% 
  summarize(mean(MaskUseProportion))

More data analysis and visualization

Let’s explore another example of group_by() and summarize() using our tibble that has all our combined information.

Instead of focusing on counties as our unit of analysis, we can use group_by() to easily switch to a state level analysis by telling the summarize() function to perform analyses for each state, combining all the rows that have the same value under the “state” column. When we combine the group_by() function with summarize(), we can easily do some pretty powerful analyses that would take a lot of effort using other programs.

cases_popsize_masked %>%
  group_by(state) %>%
  summarize(MeanMasked = mean(AlwaysMasked)) %>%
  arrange(desc(MeanMasked))

This is potentially a bad analysis because we are calculating a mean for a state based on it’s counties, and some of these counties may be represented by fewer people. So if we truly wanted the average behavior of a state, we should weight each county by its population size. Why should a county with a size of 400 contribute equally to the mean as a county of size 40,000?

The following code calculates a custom mean value, where each county is weighted by its population size:

cases_popsize_masked %>%
  group_by(state) %>%
  summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>%
  arrange(desc(WeightedMeanMasked))

Did this weighting by county size actually make a difference? Let’s store these analyses as ‘x’ and ‘y’ and compare them:

x <- cases_popsize_masked %>%
  group_by(state) %>%
  summarize(MeanMasked = mean(AlwaysMasked)) %>%
  arrange(state)
y <- cases_popsize_masked %>%
  group_by(state) %>%
  summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>%
  arrange(state)
hist(x$MeanMasked - y$WeightedMeanMasked)

It looks like on average, AlwaysMasked estimates that don’t take pop_size into account are systematically lower than those that do. When we don’t take county size into account, we effectively give more weight to smaller counties.

Summary:

New tidyverse functions used today:

  • slice() to select specific rows by index number
  • inner_join() to merge datasets based on given variable(s)
  • mutate() with str_remove to get rid of characters or words we don’t want, there are many similar functions in the stringr package, which has its own cheat sheet
  • group_by() to categorize our data by the values in a particular column (here, by state)
  • summarize() to calculate simple but very informative statistics, such as mean and variance
---
title: "R Notebook"
output: html_notebook
---

# NEW FXNS: slice(), inner_join(), summarize(), group_by()

```{r setup, include=FALSE}
knitr::opts_chunk$set(echo = TRUE)
```


This is the second session covering tidyverse commands. We will show you how to use some new functions, but we will also use some of the functions we previously used last week which will be good for extra practice. If you missed the previous session, that should not be a problem.

From last time, we looked at this [cheat sheet](https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf) to occasionally guide us.

It's typical to load all the libraries you need at the top of your code.
```{r}
library(tidyverse)
```

------
For this week, rather than use an example dataset, we are going to use several publically available datasets from the NYT github repository on the COVID-19 pandemic. This is because "wild-caught" datasets are often much messier than example datasets, so it will help us become more familiar with how you would actually go about processing data. We are going to keep our analysis purely descriptive, as it would be irresponsible to make any conclusive claims after fiddling around with R for only an hour!


## Dataset 1: mask useage

Let's load in the first data set on mask usage in the US, which is in the form of a comma separated list. This data is from a NYT survey where they asked the question: "How often do you wear a mask in public when you expect to be within six feet of another person?" (As a note, this survey was done several months ago, so these numbers are not the most up-to-date.) 

```{r}
mask_use_wide <- read_delim(file="~/Documents/GitHub/bioinformatics-coffee-hour/tidyverse/part2_winter2021/mask-use-by-county.csv", delim=",")
mask_use
```


It looks like for all the counties are represented with a 5-digit FIPS code, which is not exactly ideal because we don't know what these numbers mean. We'll get the names in a moment by combining these data with another file that also has these FIPS codes AND county names. We can thus match these data tables based on shared FIPS codes to combine them.


## Dataset 2: US case counts

In addition to the mask data, let's also load in data on COVID-19 case counts across the US, also supplied by the NYT, and combine it with our mask use data. Unlike the previous data that we loaded in from our local file system, these data we will directly download because they are so large. This will also show how you can specify web addresses instead of file names for read_delim(). This file is also comma separated, so we will let read_delim() know this.

```{r, include=FALSE}
cases <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/master/us-counties.csv", delim=",") %>%
  arrange(fips)
```

This is a lot of data. For ~3000 US counties, there's an estimate of cumulative COVID-19 cases and deaths for each day since late January (it's now March again!!!). For now, let's just look at the start of this month. 

```{r, include=FALSE}
cases_latest <- cases %>% 
  filter(date == "2021-03-01") %>%
  arrange(fips)
```

As a refresher, we are using the filter() function to select only the rows that match the string "2021-03-01" in the date column, then using the arrange() function to sort the counties by FIPS code.

-----

## Dataset 3: census data

Let's load in some additional data on population size by county, which are obtained from www.census.gov.

```{r, include=FALSE}
pop_sizes <- read_delim(file="~/Documents/GitHub/bioinformatics-coffee-hour/tidyverse/part2_winter2021/co-est2019-annres.csv.xz", delim=",")
```

When I'm working with larger files and I want to make sure they looks like I expect them to, instead of opening up the file and scrolling through all the lines, checking each by eye, I typically use various commands to just get an idea of what it looks like before proceeding. Oftentimes, this is sufficient; we don't need to check every line.

These are just a few commands I might use to look at the first and last few lines, how many rows there are overall, and also get an idea of how much missing data there might be:
```{r}
head(pop_sizes) 
nrow(pop_sizes) 
sum(!is.na(pop_sizes$'2019')) # how many counties have size estimates for 2019? it's even closer to the 3142 we expect!
tail(pop_sizes)
```

Ok so these data are a little messy and need to be cleaned up.

From these commands, we can see that the entire US was included in this table, which is not a county. We should remove that. Also, county names are all preceded with a ".", something we'll deal with in a moment. The number of rows in the table is very similar to what we expect (3,142 according to wikipedia), but maybe larger by 7 rows or so. The row containing the entire US contributes to this excess of rows beyond our expectation. 

Looking at the bottom of the table, we see some footnotes were left in. These are probably contributing to the extra number of rows and should absolutely be removed.

Let's remove these rows at the beginning and end with the **slice()** function, which pulls out rows from a data frame/tibble based on their ordinal position. It keeps all the columns, in contrast to the select() function that we discussed last week.

```{r, include=FALSE}
pop_sizes <- pop_sizes %>% dplyr::slice(2:3143) 
```

With this command, we are slicing out the middle of the data frame to remove the row with data from the whole US, as well as the footnotes at the bottom.

The 'Geographic Area' column has two pieces of info in it: county and state. We should split this column into two columns so that we can separately access the info. For instance, later on we will do an analysis not by county but by state, summing up across a state's counties, and this requires having this information in it's own separate column. To separate this info into 2 seperate columns, we will use the separate() function:

```{r, include=FALSE}
pop_sizes <- pop_sizes %>% separate(col = 'Geographic Area',
                                    into = c("County", "State"),
                                    sep = ", ")
```

Note that unlike last time we are specifying what we want to separate on with the "sep=" argument, which is a comma followed by a white space.


Let's get rid of extra characters in the new 'County' column. We don't need the "." that precedes each name, and it's pointless that each individual county name is followed by "County"... we know they're counties based on the name of the column. We can use the mutate() function to add new rows with new names, but if the name of the new row we want is the same as an existing one, it just replaces it!

```{r, include=FALSE}
pop_sizes <- pop_sizes %>% 
  mutate(County = str_remove(string = County,pattern = ".")) %>%
  mutate(County = str_remove(string = County,pattern = " County"))
```

We are combining the mutate() function with **str_remove()** which, as the name suggests, will remove a given pattern from a character column, the County column in this case.


This table includes population size data for quite a few years. Let's just get the most recent population estimate, selecting the 2019 column with the select() function, which as mentioned above pulls out specific columns:

```{r, include=FALSE}
pop_sizes <- pop_sizes %>%
  select(County, State, "2019")
```


As we did above for the data sets we used last week, we can also combine all of these individual commands into one compact command that might look something like this:
```{r, include=FALSE}
pop_sizes <- read_delim(file="~/Documents/GitHub/bioinformatics-coffee-hour/tidyverse/part2_winter2021/co-est2019-annres.csv.xz", delim=",") %>% 
  slice(2:3143) %>%
  separate(col = 'Geographic Area', into = c("County", "State"), sep = ", ") %>%
  mutate(County = str_remove(string = County,pattern = ".")) %>%
  mutate(County = str_remove(string = County,pattern = " County")) %>%
  select(County, State, "2019")
```


We can take a quick peek at these population size data, now that they're cleaned, using the summary() function, which shows there's a ton of variability in county sizes!
```{r}
summary(pop_sizes)
```



## Merging data sets with inner_join()

Let's combine these data on population sizes with our previous data on case counts and mask usage, as it may reveal interesting dynamics that vary by county size.

We will first use the join command on the case count data and the county population size data, joining by BOTH county and state. There are many different types of joining functions and it can get fairly confusing, so today we will focus just on **inner_join**. 

```{r, include=FALSE}
cases_popsize <- inner_join(x=cases_latest, y=pop_sizes, 
                                  by=c("county"="County", "state"="State"))
```

The "x=" and "y=" arguments specify which datasets we want to merge. Inner_join() returns all rows in dataset x that has matching values in dataset y, and all the columns in both x and y.

The "by=" arugment tells what variables the function should merge by, as a character vector. In other words we tell the function what columns we want to match: it compares the values in the columns in x and y, and if they match it merges them. We are matching the "county" coumn in cases_latest with the "County" column in pop_sizes, and the "state" and "State" columns in the same.

Note that because the column names (i.e. variable names) are slightly different between datasets, we have to specify which we are matching using an "="; if they had the same names, we could just list them. 


## More merging 

Now let's merge this table that has case counts and population size with the mask data! For now, let's not add in all the mask data. Let's only incorporate the the proportion of people who are "ALWAYS" masked for each county.

```{r, include=FALSE}
cases_popsize_masked <- inner_join(x=cases_popsize, 
                            y=select(mask_use_wide, c(COUNTYFP, ALWAYS)),
                            by=c("fips" = "COUNTYFP"))
```

Note that we are nesting the select() function within our inner_join() call, allowing us to subset the mask useage dataset without making an entirely new data frame.


Looking at this data table, for each county we now have cases, deaths, population size, and the proportion of people who are always masked.

You may also notice the population size data had a column entitled "2019" for size estimates during that year. Let's make this more informative and change it to "pop_size", and rename the ALWAYS column so that we know it's referring to always masked.

```{r, include=FALSE}
cases_popsize_masked <- cases_popsize_masked %>%
  rename("pop_size" = "2019", "AlwaysMasked" = ALWAYS)
```


## Visualizing data

Let's quickly probe these data just to get an idea of what they look like. The main goal here is to teach you how to use R/tidyverse commands to quickly and easily explore your data. Again, due to the context of these data, let's keep any results as fairly descriptive observations. We can't say anything conclusive without more complicated analyses that we'll leave to the public health officials.

Let's assume these case count data are accurately measuring the number of people who are getting coronavirus infections (which is obviously an underestimate, as many cases can be asymptomatic!). Assuming this, what fraction of a county's population is testing positive, and how does this fraction vary with population size? Let's use the mutate() function to add a new column with number of cases normalized by population size:

```{r}
cases_popsize_masked <- cases_popsize_masked %>%
  mutate(FracPos = cases/pop_size)
```


Do counties with large populations have a higher proportion of case counts? Let's use a simple plotting function to plot population size on the x axis and proportion of cases on y axis. Since we will look at the proportion of cases by dividing the data in the case counts column by the data in the population size column, let's just make a new column called 'FracPos' that contains this information

We can make a quick plot of these data using a base R (i.e. not tidyverse) plotting function:
```{r}
cases_popsize_masked <- cases_popsize_masked %>%
  mutate(FracPos = cases/pop_size)
plot(x=cases_popsize_masked$pop_size,
     y=cases_popsize_masked$FracPos,
     log="x",
     ylab="Fraction of cases",
     xlab="pop size")
# DON'T RUN CORRELATION ANALYSES WITH HETEROSCEDASTICITY
```

Looks interesting. Counties with larger population sizes may look like they have higher proportion of cases, but it's a little complicated because there's a lot of variability for counties with smaller population sizes. 


One thing we might be interested in are those outliers with very high rates of positive cases. What counties are theses, and in what states? We can very easily check this with the following:

```{r}
cases_popsize_masked %>%
  filter(FracPos > 0.08) %>%
  arrange(desc(FracPos))
```


Let's add another column for death rate, and visualize the relationship between the fraction of positive cases and the death rate:
```{r}
cases_popsize_masked <- cases_popsize_masked %>%
  mutate(FracDeaths = deaths/cases)
plot(x=cases_popsize_masked$FracPos,
     y=cases_popsize_masked$FracDeaths,
     log="",
     xlab="FracCases",
     ylab="FracDeaths")
```


What are these counties that have been severely impacted by deaths? Is this just noise from counties with small sizes and/or small case counts?

```{r}
cases_popsize_masked %>%
  filter(FracDeaths > 0.06) %>%
  arrange(desc(FracDeaths))
```


# Using group_by() and summarize() functions to evaluate data

The use of the group_by() and summarize() functions allows us to quickly get extremely informative information from our data. 

We'll show how they're useful in many ways, but first we need to do a little more reshaping of our data.

Let's go back to our original mask use data table, not the combined one. What if we wanted to quickly get the overall mean values of ALWAYS, ... , NEVER across all the counties? We could use the original data in wide format, and calculate the mean for each of these columns. We could also use the simple summary() function which outputs a table:

```{r}
mean(mask_use_wide$NEVER)
mean(mask_use_wide$RARELY)
mean(mask_use_wide$SOMETIMES)
mean(mask_use_wide$FREQUENTLY)
mean(mask_use_wide$ALWAYS)
summary(mask_use_wide)
```

You can do it with way, but it is kind of clunky, and it wouldn't be practical if your dataset has too many variables. It is easier to first convert the data in long format:

```{r, include=FALSE}
mask_use_long <- mask_use %>%
  pivot_longer(cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") %>%
  rename("fips" = COUNTYFP) %>%
  arrange(fips)
```

As a reminder, the "cols=" argument specifies which columns we are reshaping into rows (all *except* the "COUNTYFP" column), the "names_to=" argument specifies the name of the column that will hold the names of the reshaped columns, and the "values_to=" argument gives the name of the column that will hold the values of the reshaped columns.

We can now use the **group_by()** function, which takes our existing dataset and converts it to a grouped dataset based on a given variable, and combine it with the **summarize()** function (different from summary used above!) to get mean for each group:

```{r}
mask_use_long %>% 
  group_by(MaskUseResponse) %>% 
  summarize(mean(MaskUseProportion))
```


## More data analysis and visualization

Let's explore another example of group_by() and summarize() using our tibble that has all our combined information.

Instead of focusing on counties as our unit of analysis, we can use group_by() to easily switch to a state level analysis by telling the summarize() function to perform analyses for each state, combining all the rows that have the same value under the "state" column. When we combine the group_by() function with summarize(), we can easily do some pretty powerful analyses that would take a lot of effort using other programs.

```{r}
cases_popsize_masked %>%
  group_by(state) %>%
  summarize(MeanMasked = mean(AlwaysMasked)) %>%
  arrange(desc(MeanMasked))
```


This is potentially a bad analysis because we are calculating a mean for a state based on it's counties, and some of these counties may be represented by fewer people. So if we truly wanted the average behavior of a state, we should weight each county by its population size. Why should a county with a size of 400 contribute equally to the mean as a county of size 40,000?

The following code calculates a custom mean value, where each county is weighted by its population size:

```{r}
cases_popsize_masked %>%
  group_by(state) %>%
  summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>%
  arrange(desc(WeightedMeanMasked))
```


Did this weighting by county size actually make a difference? Let's store these analyses as 'x' and 'y' and compare them:
```{r}
x <- cases_popsize_masked %>%
  group_by(state) %>%
  summarize(MeanMasked = mean(AlwaysMasked)) %>%
  arrange(state)
y <- cases_popsize_masked %>%
  group_by(state) %>%
  summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>%
  arrange(state)
hist(x$MeanMasked - y$WeightedMeanMasked)
```

It looks like on average, AlwaysMasked estimates that don't take pop_size into account are systematically lower than those that do. When we don't take county size into account, we effectively give more weight to smaller counties. 


# Summary:
## New tidyverse functions used today:
- slice() to select specific rows by index number
- inner_join() to merge datasets based on given variable(s)
- mutate() with str_remove to get rid of characters or words we don't want, there are many similar functions in the stringr package, which has its own cheat sheet
- group_by() to categorize our data by the values in a particular column (here, by state)
- summarize() to calculate simple but very informative statistics, such as mean and variance

================================================ FILE: tidyverse_covid_data/part1/binder/Dockerfile ================================================ FROM rocker/binder:3.6.3 COPY --chown=rstudio:rstudio ./tidyverse_covid_data/part1/ ${HOME} ================================================ FILE: tidyverse_covid_data/part1/tidyverse_1.R ================================================ # always make sure you're in the correct working directory for loading/saving files! setwd("./") # previous workshop: basic R # today's workshop: tidyverse, a collection of packages # see cheat sheet here: https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf # At the top of your code, always load required packages, install if necessary #install.packages("tidyverse") library(tidyverse) # within "tidyverse", the data table used is called a "tibble" # load data using read_delim() function, which crates a tibble. mask_use <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/bde13b021e99c6b4a63fb66a6144e889cc635e31/mask-use/mask-use-by-county.csv", delim=",") mask_use # oftentimes it's useful to perform various sanity checks on your data to make sure they look like you expect sum(mask_use[1,2:6]) sum(mask_use[2,2:6]) sum(mask_use[3,2:6]) # Data tables: wide format vs long format # long format is tidy, where every column is a variable, every row is an observation # mask data looks like it's in WIDE format, let's convert it to LONG format using pivot_longer(), see cheat sheet! mask_use_long <- pivot_longer(data = mask_use, cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") # stringing functions together: pivot_longer() to convert to long format -> rename() to change column name -> arrange() to sort based on column # option 1: do each operation on a separate line mask_use_long <- pivot_longer(data = mask_use, cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") mask_use_long2 <- rename(mask_use_long, "fips" = COUNTYFP) mask_use_long3 <- arrange(mask_use_long2, "fips") # option 2: do all operations on a single line (not preferred method) mask_use_long <- pivot_longer(data = arrange(rename(mask_use, "fips" = COUNTYFP), "fips"), cols = -"fips", names_to = "MaskUseResponse", values_to = "MaskUseProportion") # option 3: the tidyverse way using the %>% pipe operator mask_use_long <- mask_use %>% pivot_longer(cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") %>% rename("fips" = COUNTYFP) %>% arrange(fips) # Let's load in case count data, also from NYT github repository cases <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/ccc8c7988a089fed287a9005e5335d8716d8db57/us-counties.csv", delim=",") %>% arrange(fips) # too much data! let's look at a particular date, August 3rd, by selecting rows using the filter() function cases_latest <- cases %>% filter(date == "2020-08-03") %>% arrange(fips) # let's combine mask data with case count data! type '?join' in console to see options # the different functions depend on how we want to treat missing data... do we have missing data? or "NA"s? cases_latest %>% filter(is.na(fips)) %>% nrow mask_use_long %>% filter(is.na(fips)) %>% nrow cases_NAs <- cases_latest %>% filter(is.na(fips)) %>% arrange() # NYC is missing FIPS number 36061! let's fix this particular cell using mutate() function, which add new columns or modifies existing ones (as we do here) cases_latest <- cases_latest %>% mutate(fips = if_else(county == "New York City", true = "36061", false = fips)) # Now that NYC FIPS data is entered, let's combine the tables with inner_join(): cases_masks <- inner_join(x=cases_latest, y=mask_use_long, by="fips") # data under "date" column all the same, not informative, and we don't need FIPS codes # we can remove columns with the select() function cases_masks <- cases_masks %>% dplyr::select(-date, -fips) # simple commands to get an idea of what the data look like! # Which counties use masks most frequently? cases_masks %>% filter(MaskUseResponse == "ALWAYS") %>% arrange(desc(MaskUseProportion), county) %>% head(n=20) # Which counties use masks least frequently? cases_masks %>% filter(MaskUseResponse == "NEVER") %>% arrange(desc(MaskUseProportion), county) %>% head(n=10) # For the counties with the most cases, how frequently are people ALWAYS wearing masks? cases_masks %>% filter(MaskUseResponse == "ALWAYS") %>% arrange(desc(cases), county) %>% head(n=10) # Summary # Key tidyverse functions used today: # - read_delim() to read files in and convert them to a tibble, a special kind of data table # - pivot_longer()/pivot_wider() to convert data between long and wide format # - rename() to change the names of particular columns # - arrange() to sort the tibble according to values in particular columns # - filter() to select particular rows that meet specific conditions # - mutate(), with if_else() to modify particular cells in a table! # - select() to select specific columns to keep ================================================ FILE: tidyverse_covid_data/part1/tidyverse_1.Rmd ================================================ --- title: "Untitled" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` ```{r} setwd("./") ``` The previous workshop gave a basic introduction to data structures in R. Today we will talk about the 'tidyverse' package, which is a collection of packages that contain many useful functions with intuitive names that are used to clean and process data sets. You don't need to use the tidyverse paclages to necessarily analyze your data in R, but it has rapidly become the industry standard. Tidyverse has many functions, especially when you see them all in a [cheat sheet](https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf). Don't worry about necessarily memorizing them all. We'll go through several of the more important ones, and as you analyze data yourselves, you can quickly find what functions may be useful based on how they're categorized here. Alternatively, google searches that include 'tidyverse' in the search may also be fast and informative. The Tidyverse package is already installed in this environment we're working in, but when you use R studio on your own computers, you'll need to install these libraries (if they aren't already installed), and then load them using the install.packages() and library() functions, respectivey: ```{r} #install.packages("tidyverse") library(tidyverse) ``` Many of the tidyverse functions operate on a data table. At the end of the previous workshop, we introduced the 'data frame', which is essentially a table with rows and columns, and the columns have informative names. When we use the functions within tidyverse, we will instead use a 'tibble', which is a table that is very similar to a data frame but has a few differences that I won't discuss in detail. Just know that when you see the word "tibble", that's basically a data table. For these next two workshops on tidyverse, we will grab data from different sources. Downloading, cleaning, and combining a data sets are very common tasks for all sorts of scientists, and these skills will be valuable for you regardless of what kind of data you want to analyze. Let's load in the first data set on mask usage in the US, recently provided by the New York Times. Let's take a peek at the raw file to get an idea of what it looks like using the head command on terminal. As you can see, it looks like a bunch of information separated by commas. Let's read in these data with the read_delim() function and let R know that our data are separated, or delimited, by commas. ```{r} mask_use <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/bde13b021e99c6b4a63fb66a6144e889cc635e31/mask-use/mask-use-by-county.csv", delim=",") mask_use ``` From the NYT github repo re. these data, they asked the question: "How often do you wear a mask in public when you expect to be within six feet of another person?" It looks like for all the counties are represented with a 5-digit FIPS code, which is not exactly ideal because we don't know what these numbers mean. We'll get the names in a moment by combining these data with another file that also has these FIPS codes AND county names. We can thus match these data tables based on shared FIPS codes to combine them. When I download other peoples data, I typically like to do many sanity checks on them to make sure they make sense. Even if you trust your source, it's always good to verify. One question that immediately came to my mind is whether these values sum to 1 for each row, as it seems like they should. We can quick check this for a few rows using the bracket notation to access particular parts of the table ```{r} sum(mask_use[1,2:6]) sum(mask_use[2,2:6]) sum(mask_use[3,2:6]) ``` # Data tables: wide format vs long format Oftentimes, it is ideal to make our data "tidy". Tidy data is data where: 1. Every column is variable. 2. Every row is an observation. 3. Every cell is a single value. When we look at this mask use data, is each column it's own variable? It doesn't seem like it. The most basic unit of analysis here is a person within a county. This person was asked a question, and they replied with one of five responses. So, we could define a single categorical variable "MaskUseResponse" that takes on one of these five values measuring approximate frequency. This could be a new column, entitled "MaskUseResponse", and in the rows beneath would be one of these five responses. Then, we could have another column that, for each of these responses, has the value of how many people wore masks that frequently. Here we are gathering the data across five columns into two columns. This used to be done with a function called gather(), which you may encounter frequently, but we'll use its newer version: pivot_longer(). (SEE CHEAT SHEET!) Also, see [here](https://en.wikipedia.org/wiki/Wide_and_narrow_data) for another simple example wide vs long format. ```{r} mask_use_long <- pivot_longer(data = mask_use, cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") ``` As you can see, the tibble is much longer and each county is represented by 5 rows, once for each value of the MaskUseResponse categorical variable. If we wanted to go from this long format back to wide format, we could use the spread() function, or its newer version pivot_wider(), to spread out these two columns into the 5 original columns, but we will not do this today. I'd also like to note that long format is not more correct or better than wide format. It completely depends on what analyses you're doing and both may be acceptable. If you load in your data set, you don't NEED to convert it to long format, but in the next workshop session I'll show you how converting to long format can potentially allow you to do some powerful stuff. Let's say right after we convert these data to long format, we want to polish it by renaming one of the columns, "COUNTYFP", to "fips", and arrange the table such that it is sorted by this column. To do this, we will use the rename() and arrange() functions, respectively. In R, we can easily do multiple operations in a row, but like all problems in coding, there are multiple, technically correct ways of doing something: ```{r, include=FALSE} # do each operation on a separate line mask_use_long <- pivot_longer(data = mask_use, cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") mask_use_long <- rename(mask_use_long, "fips" = COUNTYFP) mask_use_long <- arrange(mask_use_long, "fips") # most dense: do all operations on a single line mask_use_long <- pivot_longer(data = arrange(rename(mask_use, "fips" = COUNTYFP), "fips"), cols = -"fips", names_to = "MaskUseResponse", values_to = "MaskUseProportion") ``` When we want to do multiple operations in a row, it is generally considered better to string together multiple commands using the pipe operator in tidyverse, which is specified by %>%. This piping together of commands allows us to do many complicated things while keeping our code very easy to read. ```{r, include=FALSE} mask_use_long <- mask_use %>% pivot_longer(cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") %>% rename("fips" = COUNTYFP) %>% arrange(fips) ``` In addition to the mask data, let's also load in data on covid19 case counts across the US, also supplied by the NYT, and combine it with our mask use data. Unlike the previous data that we loaded in from our local file system, these data we will directly download because they are so large. This will also show how you can specify web addresses instead of file names for read_delim(). This file is also comma separated, so we will let read_delim() know this. ```{r, include=FALSE} cases <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/ccc8c7988a089fed287a9005e5335d8716d8db57/us-counties.csv", delim=",") %>% arrange(fips) ``` This is a lot of data. For some 3 thousand counties, there's an estimate of cumulative covid19 cases and deaths for each day since late January (it's now early August). For now, let's just look at the most recent date: 2020-08-03 in the date column. We can use the filter() function to filter our data table by row ```{r, include=FALSE} cases_latest <- cases %>% filter(date == "2020-08-03") %>% arrange(fips) ``` You'll notice that these data have both a county name and a county FIPS number, whereas the mask data only had an FIPS number. We can combine these two tables in order to get an actual county name and state for the mask data. There are several flavors of join functions (type ?join) that combine data sets based on values in a column. Here, we want to match 2 tables, 'cases_latest' and 'mask_use_long', based on FIPS number, such that rows in either table that have a matching FIPS number are combined. Which join function we choose depends on how we want to deal with missing data. For instance, what if one table doesn't have an FIPS code? If we use inner_join(), we are more conservative and the joined/combined table doesn't include any rows where either 'cases_latest' OR 'mask_use_long' had missing data. If we use left_join(), we include all the rows in the 'left' table (specified as x), and if there isn't a FIPS code in the 'right' table (specified as y), then we just fill in the gaps with NAs as needed. The other join functions follow a similar logic and you can see them in the help page. Since the outcome of using a join function depends on missing data, let's take a quick peek at how complete our data are. ```{r} cases_latest %>% filter(is.na(fips)) %>% nrow mask_use_long %>% filter(is.na(fips)) %>% nrow ``` Ok so it looks like there are 29 rows in the case count data that don't have FIPS codes... do we care about them? Let's take a peek: ```{r} cases_NAs <- cases_latest %>% filter(is.na(fips)) %>% arrange() ``` Oof, the FIPS number for New York City, perhaps one of the most important counties given the context, is missing in the case count data, yet it appears to be present in the mask use data (using a FIPS code of 36061 for Manhattan). In cases like this, we can modify the 'fips' column in the case count data to include the number 36061. Specifically, we can use the mutate() function, which allows us to change columns, and supply it with an if_else() function so that it ONLY changes values in the fips column (from NA to 36061) if the value for county in the same row is "New York City": ```{r} cases_latest <- cases_latest %>% mutate(fips = if_else(county == "New York City", true = "36061", false = fips)) ``` Now that we've modified our data table to incorporate New York City, let's use inner_join() so that we know each county has BOTH case counts and mask data. ```{r, include=FALSE} cases_masks <- inner_join(x=cases_latest, y=mask_use_long, by="fips") ``` Another thing you will notice is that there are only one cases and deaths value for each county, but these get replicated 5 times because we have 5 mask use responses for each county. This kind of looks awkward, but it's not necessarily bad to have it this way as long as we analyze our data appropriately and take this into account as we do below. Again, depending on the analysis, we could also just keep these data in wide format so that the data don't get duplicated. Since these data are all from the same date, we don't need this column anymore as it's not informative. Also, we can get rid of the county FIPS code column since we only used that information to combine the cases data and the mask data. Now that we've done that, we don't need that information either ```{r, include=FALSE} cases_masks <- cases_masks %>% dplyr::select(-date, -fips) ``` Let's use some basic tidyverse functions to superficially explore these data, just scratching the surface. Which counties use masks most frequently? ```{r} cases_masks %>% filter(MaskUseResponse == "ALWAYS") %>% arrange(desc(MaskUseProportion), county) %>% head(n=20) ``` Which counties use masks least frequently? ```{r} cases_masks %>% filter(MaskUseResponse == "NEVER") %>% arrange(desc(MaskUseProportion), county) %>% head(n=10) ``` For the counties with the most cases, how frequently are people ALWAYS wearing masks? ```{r} cases_masks %>% filter(MaskUseResponse == "ALWAYS") %>% arrange(desc(cases), county) %>% head(n=10) ``` # Summary Key tidyverse functions used today: - read_delim() to read files in and convert them to a tibble, a special kind of data table - pivot_longer()/pivot_wider() to convert data between long and wide format - rename() to change the names of particular columns - arrange() to sort the tibble according to values in particular columns - filter() to select particular rows that meet specific conditions - mutate(), with if_else() to modify particular cells in a table! - select() to select specific columns to keep This is just taking quick peeks at these data. In the next session, we'll review and learn some more tidyverse tools to manipulate data, but we'll also learn some easy-to-use functions that allow us to analyze these data in more complex ways, using relatively little code! END OF WORKSHOP, PERSONAL NOTES: What is mask use behavior like in places with many cases? Let's first quickly peek at the distributions for cases and mask-wearing ```{r} cases_masks %>% ggplot(aes(cases)) + geom_histogram() cases_masks %>% filter(MaskUseResponse == "ALWAYS") %>% ggplot(aes(MaskUseProportion)) + geom_histogram() ``` ```{r} mask_wearers <- cases_masks %>% filter(MaskUseResponse == "ALWAYS") %>% arrange(desc(MaskUseProportion), county) %>% head(n=50) mean(mask_wearers$cases) live_free_or_die <- cases_masks %>% filter(MaskUseResponse == "NEVER") %>% arrange(desc(MaskUseProportion), county) %>% head(n=50) mean(live_free_or_die$cases) ``` ================================================ FILE: tidyverse_covid_data/part2/binder/Dockerfile ================================================ FROM rocker/binder:3.6.3 COPY --chown=rstudio:rstudio ./tidyverse_covid_data/part2/ ${HOME} ================================================ FILE: tidyverse_covid_data/part2/mask-use-by-county.csv ================================================ 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56033,0.17,0.251,0.099,0.203,0.278 56035,0.223,0.111,0.061,0.231,0.374 56037,0.061,0.295,0.23,0.146,0.268 56039,0.095,0.157,0.16,0.247,0.34 56041,0.098,0.278,0.154,0.207,0.264 56043,0.204,0.155,0.069,0.285,0.287 56045,0.142,0.129,0.148,0.207,0.374 ================================================ FILE: tidyverse_covid_data/part2/tidyverse_2.R ================================================ # This is the second session covering tidyverse commands. # From last time, we looked at this cheat sheet: https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf # Let's quickly reload the two data sets we used last time and clean them up. # load mask data mask_use_wide <- read_delim(file="mask-use-by-county.csv", delim=",") mask_use_long <- mask_use_wide %>% pivot_longer(cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") %>% rename("fips" = COUNTYFP) %>% arrange(fips) # load case count data, filter based on most recent date cases <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/master/us-counties.csv", delim=",") cases_latest <- cases %>% filter(date == "2020-08-03") %>% dplyr::select(-date) %>% arrange(fips) # These data from last time are all at the county level, which vary considerably by size. # Let's load in some additional data on population size by county, obtained from www.census.gov. pop_sizes <- read_delim(file="co-est2019-annres.csv.xz", delim=",") # Always double check file to make sure it looks alright! head(pop_sizes) nrow(pop_sizes) sum(!is.na(pop_sizes$'2019')) # how many counties have size estimates for 2019? it's even closer to the 3142 we expect! tail(pop_sizes) # These data are a little messy and need to be cleaned up! Let's do this with tidyverse commands. # Let's remove rows that we don't need using the slice() function pop_sizes <- pop_sizes %>% dplyr::slice(2:3143) # this also gets rid of the notes at the bottom # The 'Geographic Area' column has two pieces of info in it: county and state. Let's split this into 2 separate columnds using the separate() function. pop_sizes <- pop_sizes %>% separate(col = 'Geographic Area', into = c("County", "State"), sep = ", ") # Let's get rid of extra characters in the new 'County' column. We can use the mutate() function along with another function called str_remove(). pop_sizes <- pop_sizes %>% mutate(County = str_remove(string = County,pattern = ".")) %>% mutate(County = str_remove(string = County,pattern = " County")) # Let's use only the most recent estimate of county population size using the select() function. pop_sizes <- pop_sizes %>% dplyr::select(County, State, "2019") # OPTIONAL: we can combine all of our cleaning/filtering commands into one chain of commands to send to others: pop_sizes <- read_delim(file="co-est2019-annres.csv", delim=",") %>% dplyr::slice(2:3143) %>% separate(col = 'Geographic Area', into = c("County", "State"), sep = ", ") %>% mutate(County = str_remove(string = County,pattern = ".")) %>% mutate(County = str_remove(string = County,pattern = " County")) %>% dplyr::select(County, State, "2019") # The summary() function gives us a quick peek at these data. NOTE: summary() is very different from the summarize() function that we'll cover below. summary(pop_sizes) # Combining our previous data on case counts with county size cases_popsize <- inner_join(x=cases_latest, y=pop_sizes, by=c("county"="County", "state"="State")) # Combining these data with mask usage data cases_popsize_masked <- inner_join(x=cases_popsize, y=dplyr::select(mask_use_wide, c(COUNTYFP, ALWAYS)), by=c("fips" = "COUNTYFP")) # Renaming a coupe of columns to something more informative: cases_popsize_masked <- cases_popsize_masked %>% rename("pop_size" = "2019", "AlwaysMasked" = ALWAYS) ## # Visualizing data and finding outliers ## # What is the relationship between the fraction of positive cases and the population size of the county? Do counties with large populations have a higher proportion of case counts? # We will make our first plot the the "base" R function plot() cases_popsize_masked <- cases_popsize_masked %>% mutate(FracPos = cases/pop_size) plot(x=cases_popsize_masked$pop_size, y=cases_popsize_masked$FracPos, log="x", ylab="Fraction of cases", xlab="pop size") # DON'T RUN CORRELATION ANALYSES WITH HETEROSCEDASTICITY # What are those outliers with high rates of positive cases? What counties/states are these? cases_popsize_masked %>% filter(FracPos > 0.08) %>% arrange(desc(FracPos)) # Let's add another column for death rate, and visualize the relationship between the fraction of positive cases and the death rate: cases_popsize_masked <- cases_popsize_masked %>% mutate(FracDeaths = deaths/cases) plot(x=cases_popsize_masked$FracPos, y=cases_popsize_masked$FracDeaths, log="", xlab="FracCases", ylab="FracDeaths") # What are these counties that have been severely impacted by deaths rates? Is this just noise from small population sizes? cases_popsize_masked %>% filter(FracDeaths > 0.15) %>% arrange(desc(FracDeaths)) ## # group_by() and summarize() functions are extremely easy and powerful ## # The use of the group_by() and summarize() functions allows us to quickly get extremely informative information from our data. # One way to get the mean values for the different mask use frequency categories: mean(mask_use_wide$NEVER) mean(mask_use_wide$RARELY) mean(mask_use_wide$SOMETIMES) mean(mask_use_wide$FREQUENTLY) mean(mask_use_wide$ALWAYS) summary(mask_use_wide) # Alternatively, we can convert our data into long format (which we did above) and use the group_by() and summarize() functions to quickly do useful analyses: mask_use_long %>% group_by(MaskUseResponse) %>% summarize(mean(MaskUseProportion)) # Looking at the cheat sheet, you will see summarize can take a variety of functions. # Instead of analyzing data by county, we can easily switch to a state-level analysis using group_by() and sumamrize() cases_popsize_masked %>% group_by(state) %>% summarize(MeanMasked = mean(AlwaysMasked)) %>% arrange(desc(MeanMasked)) # Calculating the mean value for a state using counties of very different sizes is bad; mean() treats each county equally, but in reality they vary tremendously in size # Let's compute a custom weighted mean cases_popsize_masked %>% group_by(state) %>% summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>% arrange(desc(WeightedMeanMasked)) # Did this weighting by county size actually make a difference? Let's store these analyses as 'x' and 'y' and compare them: x <- cases_popsize_masked %>% group_by(state) %>% summarize(MeanMasked = mean(AlwaysMasked)) %>% arrange(state) y <- cases_popsize_masked %>% group_by(state) %>% summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>% arrange(state) hist(x$MeanMasked - y$WeightedMeanMasked) # The histogram suggests smaller counties have, on average, lower values for "AlwaysMasked"? Ploe the variables to verify: plot(x=cases_popsize_masked$pop_size, y=cases_popsize_masked$AlwaysMasked, log="x", xlab="pop size", ylab="AlwaysMasked") # Summary: ## New tidyverse functions used today: - slice() to select specific rows by index number - separate() to take a column with multiple pieces of information and split it into multiple columns - mutate() with str_remove to get rid of characters or words we don't want, there are many similar functions in the stringr package, which has its own cheat sheet - select() to - group_by() to categorize our data by the values in a particular column (here, by state) - summarize() to calculate simple but very informative statistics, such as mean and variance ================================================ FILE: tidyverse_covid_data/part2/tidyverse_2.Rmd ================================================ --- title: "Untitled" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) ``` This is the second session covering tidyverse commands. We will show you how to use some new functions, but we will also use some of the functions we previously used last week which will be good for extra practice. If you missed the previous session, that should not be a problem. From last time, we looked at this [cheat sheet](https://rstudio.com/wp-content/uploads/2015/02/data-wrangling-cheatsheet.pdf) to occasionally guide us. It's typical to load all the libraries you need at the top of your code. ```{r} library(tidyverse) ``` Let's quickly reload the two data sets we used last time and clean them up. As a reminder, we converted the mask use data from wide format into long format, but let's keep the data in both versions today. ```{r, include=FALSE} # load mask data mask_use_wide <- read_delim(file="mask-use-by-county.csv", delim=",") mask_use_long <- mask_use_wide %>% pivot_longer(cols = -COUNTYFP, names_to = "MaskUseResponse", values_to = "MaskUseProportion") %>% rename("fips" = COUNTYFP) %>% arrange(fips) # load case count data, filter based on most recent date cases <- read_delim(file="https://github.com/nytimes/covid-19-data/raw/master/us-counties.csv", delim=",") cases_latest <- cases %>% filter(date == "2020-08-03") %>% dplyr::select(-date) %>% arrange(fips) ``` These data from last time are all at the county level, which vary considerably by size. So for instance if we wanted to know the fraction of positive cases in a state, or if we wanted to know what mask-wearing behavior was in a particular state, we should really take this variability in county size into account. It's also useful to see which counties have been hit particularly hard, since any positive cases may be expected if the county is quite large. Let's load in some additional data on population size by county, which I obtained from www.census.gov. ```{r, include=FALSE} pop_sizes <- read_delim(file="co-est2019-annres.csv.xz", delim=",") ``` When I'm working with larger files and I want to make sure they looks like I expect them to, instead of opening up the file and scrolling through all the lines, checking each by eye, I typically use various commands to just get an idea of what it looks like before proceeding. Oftentimes, this is sufficient; we don't need to check every line. These are just a few commands I might use to look at the first and last few lines, how many rows there are overall, and also get an idea of how much missing data there might be: ```{r} head(pop_sizes) nrow(pop_sizes) sum(!is.na(pop_sizes$'2019')) # how many counties have size estimates for 2019? it's even closer to the 3142 we expect! tail(pop_sizes) ``` Ok so these data are a little messy and need to be cleaned up. From these commands, we can see that the entire US was included in this table, which is not a county. We should remove that. Also, county names are all preceded with a ".", something we'll deal with in a moment. The number of rows in the table is very similar to what we expect (3,142 according to wikipedia), but maybe larger by 7 rows or so. The row containing the entire US contributes to this excess of rows beyond our expectation. Looking at the number of entries for the year 2019, they're extremely close to what we expect. Looking at the bottom of the table, we see some footnotes were left in. These are probably contributing to the extra number of rows and should absolutely be removed. Let's remove these rows at the beginning and end with the slice() function (unlike the select() function that removes columns). ```{r, include=FALSE} pop_sizes <- pop_sizes %>% dplyr::slice(2:3143) # this also gets rid of the notes at the bottom ``` As you can see in the cheat sheet, there are many ways to select rows, even at random! We won't do this, but this could be useful if you wanted to set up an analysis on a smaller subset of your data to make sure everything runs correctly but quickly. Then, after everything is set up, run your analyses on the entire data, which could take a while with huge data sets. The 'Geographic Area' column has two pieces of info in it: county and state. We should split this column into two columns so that we can separately access the info. For instance, later on we will do an analysis not by county but by state, summing up across a state's counties, and this requires having this information in it's own separate column. To separate this info into 2 seperate columns, we will use the separate() function: ```{r, include=FALSE} pop_sizes <- pop_sizes %>% separate(col = 'Geographic Area', into = c("County", "State"), sep = ", ") ``` This illustrates how even though we split our data up by commas when we loaded it in above, we can further split columns based on specific characters. Above, we asked the read_delim() function to split by commas, so you would think this column/field would also get separated, but when you look at the raw data file you can see that data from each column is contained within quotes, and this info within quotes is separated by commas. And just so you know, there's a related function called unite() that does just the opposite: combining columns into a single column. Let's get rid of extra characters in the new 'County' column. We don't need the "." that precedes each name, and it's pointless that each individual county name is followed by "County"... we know they're counties based on the name of the column. We can use the mutate() function to add new rows with new names, but if the name of the new row we want is the same as an existing one, it just replaces it! ```{r, include=FALSE} pop_sizes <- pop_sizes %>% mutate(County = str_remove(string = County,pattern = ".")) %>% mutate(County = str_remove(string = County,pattern = " County")) ``` This table includes population size data for quite a few years. Let's just get the most recent population estimate, selecting the 2019 column with the select() function: ```{r, include=FALSE} pop_sizes <- pop_sizes %>% dplyr::select(County, State, "2019") ``` As we did above for the data sets we used last week, we can also combine all of these individual commands into one compact command that might look something like this: ```{r, include=FALSE} pop_sizes <- read_delim(file="co-est2019-annres.csv", delim=",") %>% dplyr::slice(2:3143) %>% separate(col = 'Geographic Area', into = c("County", "State"), sep = ", ") %>% mutate(County = str_remove(string = County,pattern = ".")) %>% mutate(County = str_remove(string = County,pattern = " County")) %>% dplyr::select(County, State, "2019") ``` We can take a quick peek at these population size data, now that they're cleaned, using the summary() function, which shows there's a ton of variability in county sizes! ```{r} summary(pop_sizes) ``` Let's combine these data on population sizes with our previous data on case counts and mask usage, as it may reveal interesting dynamics that vary by county size. We will first use the join command on the case count data and the county population size data, joining by BOTH county and state. Why can't we just join by county? ```{r, include=FALSE} cases_popsize <- inner_join(x=cases_latest, y=pop_sizes, by=c("county"="County", "state"="State")) ``` Using this table with case counts and population size, let's add in the mask data. For now, let's not add in all the mask data. Let's only incorporate the the proportion of people who are "ALWAYS" masked for each county ```{r, include=FALSE} cases_popsize_masked <- inner_join(x=cases_popsize, y=dplyr::select(mask_use_wide, c(COUNTYFP, ALWAYS)), by=c("fips" = "COUNTYFP")) ``` Looking at this data table, for each county we now have cases, deaths, population size, and the proportion of people who are always masked. You may also notice the population size data had a column entitled "2019" for size estimates during that year. Let's make this more informative and change it to "pop_size", and rename the ALWAYS column so that we know it's referring to always masked. ```{r, include=FALSE} cases_popsize_masked <- cases_popsize_masked %>% rename("pop_size" = "2019", "AlwaysMasked" = ALWAYS) ``` # Visualizing data and finding outliers Let's quickly probe these data just to get an idea of what they look like. The main goal here is to teach you how to use R/tidyverse commands to quickly and easily explore your data. Again, due to the context of these data, let's keep any results as descriptive observations. We can't say anything conclusive without more complicated analyses that we'll leave to the public health officials. Let's assume these case count data are accurately measuring the number of people who are getting coronavirus infections (which is a horrible assumption because TONS of younger people may be completely asymptomatic and never get tested). Assuming this, what fraction of a county's population is testing positive, and how does this fraction vary with population size? ```{r} cases_popsize_masked <- cases_popsize_masked %>% mutate(FracPos = cases/pop_size) ``` Do counties with large populations have a higher proportion of case counts? Let's use a simple plotting function to plot population size on the x axis and proportion of cases on y axis. Since we will look at the proportion of cases by dividing the data in the case counts column by the data in the population size column, let's just make a new column called 'FracPos' that contains this information We can make a quick plot of these data using a base R (i.e. not tidyverse) plotting function: ```{r} cases_popsize_masked <- cases_popsize_masked %>% mutate(FracPos = cases/pop_size) plot(x=cases_popsize_masked$pop_size, y=cases_popsize_masked$FracPos, log="x", ylab="Fraction of cases", xlab="pop size") # DON'T RUN CORRELATION ANALYSES WITH HETEROSCEDASTICITY ``` Looks interesting. Counties with larger population sizes may look like they have higher proportion of cases, but it's a little complicated because there's a lot of variability for counties with smaller population sizes. One thing that might catch our eye are those outliers with very high rates of positive cases? What counties are theses, and in what states? We can very easily check this with the following: ```{r} cases_popsize_masked %>% filter(FracPos > 0.08) %>% arrange(desc(FracPos)) ``` Let's add another column for death rate, and visualize the relationship between the fraction of positive cases and the death rate: ```{r} cases_popsize_masked <- cases_popsize_masked %>% mutate(FracDeaths = deaths/cases) plot(x=cases_popsize_masked$FracPos, y=cases_popsize_masked$FracDeaths, log="", xlab="FracCases", ylab="FracDeaths") ``` What are these counties that have been severely impacted by deaths? Is this something to be concerned about, or is this just noise from counties with small sizes and/or small case counts? ```{r} cases_popsize_masked %>% filter(FracDeaths > 0.15) %>% arrange(desc(FracDeaths)) ``` # group_by() and summarize() functions are extremely easy and powerful The use of the group_by() and summarize() functions allows us to quickly get extremely informative information from our data. We'll show how they're useful in many ways, but I'd like to start with showing the importance of changing your data from long format to wide format. Let's go back to our original mask use data table, not the combined one. What if we wanted to quickly get the overall mean values of ALWAYS, ... , NEVER across all the counties? We could use the original data in wide format, and calculate the mean for each of these columns. We could also use the simple summary() function which outputs a table: ```{r} mean(mask_use_wide$NEVER) mean(mask_use_wide$RARELY) mean(mask_use_wide$SOMETIMES) mean(mask_use_wide$FREQUENTLY) mean(mask_use_wide$ALWAYS) summary(mask_use_wide) ``` There's nothing wrong with doing it this way, but it's not very elegant and would get more difficult if our data were more complicated. Also, the summary function outputs a table that is not the easiest to analyze. Another way of calculating these values is to convert our mask data into long format where these mask-wearing frequency categories (NEVER, ... , ALWAYS) are different values of a categorical variable (MaskUseResponse), and we tell R that we want to do an analysis separately for each of these different categorical variable values, where here this analysis is just calculating the mean. In other words, for all the rows with values of NEVER for MaskUseResponse, calculate a mean separately, and repeat for all the other possible MaskUseResponse values. We tell R to treat these different categorical variable values separately using group_by(), and the summarize() function can then use these groupings to perform analyses, here calculating the mean. ```{r} mask_use_long %>% group_by(MaskUseResponse) %>% summarize(mean(MaskUseProportion)) ``` As you can see, this also outputs a tibble, which we can use for other downstream analyses. Looking at the cheat sheet, you will see summarize can take a variety of functions. Let's explore another example of group_by() and summarize() using our tibble that has all our combined information. Instead of focusing on counties as our unit of analysis, we can use group_by() to easily switch to a state level analysis by telling the summarize() function to perform analyses for each state, combining all the rows that have the same value under the "state" column. When we combine the group_by() function with summarize(), we can easily do some pretty powerful analyses that would take a lot of effort using other programs. ```{r} cases_popsize_masked %>% group_by(state) %>% summarize(MeanMasked = mean(AlwaysMasked)) %>% arrange(desc(MeanMasked)) ``` You can comment out the line containing the group_by() statement to see it's effect. Note that this creates a new tibble, and that a new column is created called "MeanMasked" that calculates the mean as a function of whatever variable was put into group_by(). So what is happening here is because we gave "state" to group_by(), R goes through all the rows of AlwaysMasked and if they have the same value for "state", from different counties from the same state, it uses all that data for the state to calculate a mean. I'd like to point out that this is potentially a bad analysis because we are calculating a mean for a state based on it's counties, and some of these counties may be represented by fewer people. So if we truly wanted the average behavior of a state, we should weight each county by its population size. Why should a county with a size of 400 contribute equally to the mean as a county of size 40,000? The following code calculates a custom mean value, where each county is weighted by its population size: ```{r} cases_popsize_masked %>% group_by(state) %>% summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>% arrange(desc(WeightedMeanMasked)) ``` See the element-wise vector math figure provided in the github repository for a graphical explanation of what's being done here: Did this weighting by county size actually make a difference? Let's store these analyses as 'x' and 'y' and compare them: ```{r} x <- cases_popsize_masked %>% group_by(state) %>% summarize(MeanMasked = mean(AlwaysMasked)) %>% arrange(state) y <- cases_popsize_masked %>% group_by(state) %>% summarize(WeightedMeanMasked = sum(AlwaysMasked*pop_size)/sum(pop_size)) %>% arrange(state) hist(x$MeanMasked - y$WeightedMeanMasked) ``` It looks like on average, AlwaysMasked estimates that don't take pop_size into account are systematically lower than those that do. When we don't take county size into account, we effectively give more weight to smaller counties. Thus, if by doing this we produce lower estimates of AlwaysMasked by state, this suggests that smaller counties in general have lower values for AlwaysMasked. Is this true? ```{r} plot(x=cases_popsize_masked$pop_size, y=cases_popsize_masked$AlwaysMasked, log="x", xlab="pop size", ylab="AlwaysMasked") ``` # Summary: ## New tidyverse functions used today: - slice() to select specific rows by index number - separate() to take a column with multiple pieces of information and split it into multiple columns - mutate() with str_remove to get rid of characters or words we don't want, there are many similar functions in the stringr package, which has its own cheat sheet - select() to - group_by() to categorize our data by the values in a particular column (here, by state) - summarize() to calculate simple but very informative statistics, such as mean and variance ================================================ FILE: unix-pipes/binder/environment.yml ================================================ channels: - conda-forge - bioconda - defaults dependencies: - bash_kernel - bwa - fastp - samtools - time ================================================ FILE: unix-pipes/index.ipynb ================================================ { "cells": [ { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "## Unix Pipes\n", "### Bioinformatics Coffee Hour - June 16, 2020\n", "#### Your Host: Nathan Weeks" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "## What this lesson will cover\n", "* Unix pipes in shell scripts to improve efficiency of simple bioinformatics workflows\n", "* _Example:_ Short-read (FASTQ) alignment to BAM " ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "subslide" } }, "source": [ "### Software Utilities Used" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "fragment" } }, "source": [ "#### curl\n", " https://curl.haxx.se/\n", "command line tool and library for transferring data with URLs" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "fragment" } }, "source": [ "#### gzip\n", " https://www.gnu.org/software/gzip/\n", "popular data compression program" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "subslide" } }, "source": [ "#### fastp\n", " https://github.com/OpenGene/fastp\n", "all-in-one FASTQ preprocessor (QC/adapters/trimming/filtering/splitting/merging...)" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "fragment" } }, "source": [ "#### bwa\n", " http://bio-bwa.sourceforge.net/\n", "software package for mapping low-divergent sequences against a large reference genome" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "fragment" } }, "source": [ "#### samtools\n", " https://github.com/samtools/samtools\n", "tools... for manipulating [Reading/writing/editing/indexing/viewing] next-generation sequencing data [SAM/BAM/CRAM format]" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "## Motivation\n", "Bioinformatics workflows are usually *big*...\n", " - big input files\n", " - many workflow **stages**\n", " + each workflow stage usually takes as input a file(s) from the previous stage, and produces output file(s) for the next stage\n", " + each stage may use a different software tool" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "## Example: 1/2 of a variant-calling pipeline\n", "\n", "\n", "*source: Brian Arnold, https://github.com/harvardinformatics/shortRead_mapping_variantCalling/ *" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "### Definition\n", "
\n", "
file
\n", "
An object that can be written to, or read from, or both.
\n", "
\n", "\n", "*source: POSIX.1-2018*" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "#### Unix processes start with 3 open \"files\" (connected to a terminal in an interactive shell)\n", "
\n", "
standard input (\"stdin\")
\n", "
An input stream usually intended to be used for primary data input.
\n", "
standard output (\"stdout\")
\n", "
An output stream usually intended to be used for primary data output.
\n", "
standard error (\"stderr\")
\n", "
An output stream usually intended to be used for diagnostic messages.
\n", "
\n", "\n", "![process](images/process.svg)" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "
\n", "
filter
\n", "
A command whose operation consists of reading data from standard input or a list of input files and writing data to standard output.\n", " Typically, its function is to perform some transformation on the data stream.
\n", "
\n", "\n", "source: POSIX.1-2018_\n", "\n", "_Many unix programs act as filters_" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "fragment" } }, "source": [ "## Example: grep\n", "\n", "When given only a pattern argument (no file arguments), grep operates as a _filter_, reading input from stdin, and writing output to stdout (_enter CTRL-D to indicate end of input_)\n", "\n", "```\n", "$ grep PATTERN\n", "```" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "fragment" } }, "source": [ "### Redirect stdout to a file\n", "\n", "To write grep's output to a file (instead of a terminal), use the shell redirection operator (`>`)\n", "\n", "```\n", "$ grep PATTERN > matched.txt\n", "```" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "### Introducing Pipes\n", "Pipes are a form of [Interprocess Communication](https://en.wikipedia.org/wiki/Inter-process_communication) with the following properties:\n", "* One-way commmunication channel between two _processes_\n", " - **process**: a running program\n", "* Data written (sequentially) by one process (usually to its stdout) is read (sequentially) by another (usually from its stdin)\n", "* Operating system kernel blocks writer (process in a \"sleep\" state) until data is read by reader\n", " + Similarly, reader blocks until writer writes data" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "## Pipes in Programming Languages \n", "* The (Unix/Linux/POSIX) shell command language (understood by bash, zsh, sh, and others) has a construct called a pipe (`|` operator)\n", "* Many other programming languages allow use of pipes in some fashion:\n", " - AWK: `command | getline [var]` to read, `print ... > command` to \n", " - Python: [subprocess.Popen()](https://docs.python.org/3/library/subprocess.html#replacing-shell-pipeline)\n", " - R: `pipe()`\n", " + Not to be confused with the pipe `%>%` operator from the magrittr package" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "### (shell) Pipeline\n", "* A sequence of commands separated by the `|` (pipe) operator\n", "* standard output of the command on the left of the `|` is connected to the standard input of the command to the right of the `|`" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "## Why use pipes?\n", "\n", "1. I/O efficiency\n", " - Data flows between processes without being written to disk\n", "2. Concurrency\n", " - Processes in a pipeline execute concurrently\n", " - e.g., utilizing multicore processors" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "subslide" } }, "source": [ "### Example: Sequential Laundry\n", "![laundry1](images/laundry1.gif)\n", "*source: https://cs.stanford.edu/people/eroberts/courses/soco/projects/risc/pipelining/index.html*" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "subslide" } }, "source": [ "### Example: Pipelined Laundry\n", "![laundry2](images/laundry2.gif)\n", "*source: https://cs.stanford.edu/people/eroberts/courses/soco/projects/risc/pipelining/index.html*" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "---\n", "## Examples\n", "---" ] }, { "cell_type": "markdown", "metadata": { "slideshow": { "slide_type": "slide" } }, "source": [ "#### Example 1: download FASTA file and compress on-the-fly" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "mkdir -p input\n", "GENOME_FASTA_URL=https://github.com/harvardinformatics/shortRead_mapping_variantCalling/raw/b120a823c23b1eaf1cfb95ea5d5ca0ce26a50c32/data/genome/Tgut_subseg_renamed.fa\n", "curl -sL ${GENOME_FASTA_URL} | gzip -v -9 > input/Tgut_subseg_renamed.fa.gz\n", "ls -lh input/Tgut_subseg_renamed.fa.gz" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "##### Explanation: \n", "* `curl` downloads the FASTA file from the specified URL and writes it to its stdout.\n", "* `gzip`, when not given a file argument, reads (uncompressed) data from stdin and writes compressed data to stdout.\n", " - The `gzip -v` option prints the compression ratio to stderr_, the `-9` option specififies maximal compression (==more run time, but better compression)\n", "* The shell output redirection operator (`>`) writes the stdout of `gzip` to a file (Tgut_subseg_renamed.fa.gz)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "#### Example 2: Print the length of each sequence in the compressed FASTA file\n", "Our FASTA file has one sequence per line. [AWK](https://en.wikipedia.org/wiki/AWK) can generate a tab-separated list of sequence ID and sequence length (\n", "\n", "_Problem:_ AWK cannot read a gzip file directly.\n", "\n", "_Solution:_ Combine `gzip` options`-d` (decompress) and `-c` (write to stdout), and pipe to awk's stdin:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "gzip -dc input/Tgut_subseg_renamed.fa.gz |\n", " awk '/^>/ { if (len) print len; len = 0; printf(\"%s\\t\", substr($1,2)) }\n", " !/^>/ { len+=length }\n", " END { printf(\"%i\\n\", len) }'" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Fetch sequence reads to align:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "(cd input && curl -LO 'https://github.com/harvardinformatics/shortRead_mapping_variantCalling/raw/5530f1991da66af82d0213bc3492da8a431a1a92/data/fastq/ERR1013163_[1-2].fastq.gz')" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Next, index the genome file so `bwa` can align reads to it:\n", "\n", "_Note: `bwa` can index (gzip-)compressed reference sequences_" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "bwa index input/Tgut_subseg_renamed.fa.gz" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Sequence alignment workflow (sequential)\n", "\n", "* Output of each stage written as file(s)\n", "* Command in each stage must be run to completion before next stage is executed" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "set -o xtrace\n", "mkdir -p orig\n", "time (\n", "cd orig\n", "ln -sf ../input\n", "# trim reads, generate HTML report\n", "fastp --in1 input/ERR1013163_1.fastq.gz --in2 input/ERR1013163_2.fastq.gz --out1 ERR1013163_1.fastq-trimmed.gz --out2 ERR1013163_2.fastq-trimmed.gz\n", "# align reads\n", "bwa mem input/Tgut_subseg_renamed.fa.gz ERR1013163_1.fastq-trimmed.gz ERR1013163_2.fastq-trimmed.gz > ERR1013163.sam\n", "# sort alignments by read name\n", "samtools sort -n --output-fmt bam -o ERR1013163.bam ERR1013163.sam \n", "# fill in mate coordinates and insert size in BAM records\n", "samtools fixmate -m ERR1013163.bam ERR1013163-fixmate.bam\n", "# sort by alignment coordinate\n", "samtools sort -o ERR1013163-fixmate-sort.bam ERR1013163-fixmate.bam\n", "# mark duplicate alignments\n", "samtools markdup -f markdup.stats --output-fmt-option level=9 ERR1013163-fixmate-sort.bam out.bam\n", "# index resulting bam file \n", "samtools index out.bam\n", "samtools stats out.bam > out.bam.stats.txt\n", ")\n" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Check files created :" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "ls -lh orig" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## A special \"file\": /dev/stdin\n", "\n", "* Some utilities either accept an optional file argument (e.g., gzip, awk, most other unix utilities), reading from stdin if no input file is specified\n", "* Some utilities accept \"-\" in place of either an input or output file argument to mean stdin (or stdout) respectively\n", "* Other utilities require an actual file argument; e.g. `bwa mem`:\n", "```\n", " bwa mem [options] db.prefix reads.fq [mates.fq]\n", "```\n", " - **reads.fq** is required. However, `bwa mem` reads **reads.fq** sequentially, and can conceptually use a pipe.\n", " \n", "```\n", " fastp reads.fq | bwa mem db.prefix /dev/stdin\n", "```\n", " \n", "**Note: this works only if the utility reads (or writes) the file sequentially**" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Sequence alignment workflow (pipeline)\n", "\n", "* Process in each stage writes output directly to input of another process(es)\n", "* Processes execute concurrently" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "mkdir -p ex-pipe\n", "time (\n", "cd ex-pipe\n", "ln -s ../input\n", "# fastp supports interleaved (https://github.com/OpenGene/fastp#output-to-stdout) output\n", "fastp --in1 input/ERR1013163_1.fastq.gz --in2 input/ERR1013163_2.fastq.gz --stdout 2>/dev/null |\n", " # bwa mem requires a FASTQ file operand (will not take reads from stdin).\n", " # /dev/stdin is a special file that represents a process's standard input\n", " bwa mem input/Tgut_subseg_renamed.fa.gz /dev/stdin 2> /dev/null |\n", " # sort input SAM records by read name (\"-n\"), write uncompressed BAM ((compression) level=0) to stdout\n", " # (otherwise, samtools will detect input format & use same output format)\n", " samtools sort -n --output-fmt bam --output-fmt-option level=0 |\n", " # Usage: samtools fixmate \n", " # samtools allows \"-\" to be used in place of \n", " samtools fixmate -m - - |\n", " # samtools sort reads from stdin and writes to stdout by default\n", " samtools sort |\n", " samtools markdup -f markdup.stats --output-fmt-option level=9 - - |\n", " # samtools index requires an input bam file argument; use /dev/stdin\n", " tee out.bam >(samtools index /dev/stdin out.bam.bai) |\n", " # samtools stats reads from stdin and writes to stdout by default\n", " samtools stats > out.bam.stats.txt\n", ")" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Much less data written to / read from disk:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "ls -lh ex-pipe" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Let's take a closer look at the last stages of the pipeline.\n", "\n", "Two additional tools are being introduced: the [tee](https://en.wikipedia.org/wiki/Tee_(command)) command, and [process substitution](https://en.wikipedia.org/wiki/Process_substitution) ( `>(...)` )\n", "\n", "### tee\n", "\n", "`tee` duplicates its stdin to one or more files in addition to stdout:\n", "\n", "![tee](https://upload.wikimedia.org/wikipedia/commons/2/24/Tee.svg)\n", "\n", "*source: Wikipedia*" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "```\n", "... |\n", " samtools markdup -f markdup.stats --output-fmt-option level=9 - - |\n", " tee out.bam >(samtools index /dev/stdin out.bam.bai) |\n", " samtools stats > out.bam.stats.txt\n", "```\n", "![pipe](images/pipe.svg)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# The End!\n", "## Questions, comments...?" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "---\n", "## Bonus material\n", "---" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### pipefail\n", "By convention, processes return an exit status of 0 to indicate success, and > 0 to indicate failure.\n", "\n", "By default, the exit status of a pipeline is the exit status of the last command in the pipeline:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "samtools sort foo/bar.sam | head\n", "echo \"exit status: $?\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "This is inconvenient when trying to determine if a (Slurm) job script ran successfully, or if it's desired to terminate the script if any command fails (e.g., using `set -o errexit`).\n", "\n", "The `pipefail` option causes the exit status of a pipeline to be the exit status of the last command in the pipeline to have a non-zero exit status:" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "set -o pipefail\n", "samtools sort foo/bar.sam | head\n", "echo \"exit status: $?\"" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## Other Terms\n", "\n", "### Regular File\n", "> A file that is a _**randomly accessible**_ sequence of bytes.\n", "```\n", "$ ls -lh ERR1013163_1.fastq.gz\n", "-rw-r--r-- 1 jovyan root 3.8M Jun 10 16:00 ERR1013163_1.fastq.gz\n", "\n", "### FIFO (aka \"named pipe\")\n", "**F**irst **I**n **F**irst **O**ut\n", "\n", "> A type of file with the property that data written to such a file is read on a first-in-first-out basis.\n", "\n", "_In other words, data are written / read **sequentially**_\n", "\n", "### FIFO Example" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "set -o xtrace\n", "mkfifo test.fifo\n", "printf 'line1\\nline2\\nline3\\n' > test.fifo &\n", "sleep 5\n", "jobs\n", "sleep 5\n", "cat test.fifo" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### Process Substitution\n", "`command1 >(command2)` is loosely analogous to:\n", "```\n", "mkfifo tmp.fifo\n", "command1 > tmp.fifo &\n", "command2 < tmp.fifo\n", "rm tmp.fifo\n", "```" ] } ], "metadata": { "kernelspec": { "display_name": "Bash", "language": "bash", "name": "bash" }, "language_info": { "codemirror_mode": "shell", "file_extension": ".sh", "mimetype": "text/x-sh", "name": "bash" } }, "nbformat": 4, "nbformat_minor": 4 }